Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

高麗初期 鄕吏職의 由來에 對한 小考 : 鄕吏職名의 變化를 中心으로

趙榮濟 부산대사학회 1980 역사와 세계 Vol.- No.4

榮山江 河口 댐 建設前後의 海洋環境 比較硏究

曺炷煥,朴榮濟 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

In order to find out the effect of the enclosure at Yeongsan River Estuary on the surrounding aquatic envionments, a study on the physical, chemical and biological changes in this area had been carried out during from February, 1979 to November, 1985. Since November, 1981 when the dam was completed, there have been the considerable changes in the enclosed estuary in salinity, dissolved oxygen density, COD, nutrients, suspended particulate matter(SPM), transparency and biological compositions, with reduced water flow and appearance of immovable water masses. The relationship between the density of SPM and the transparency was calculated as S=86.4982-71.4837T(r=-0.8610) in 1979, but this was changed after the enclosure of the tam to S=22.0384-6.1865(r=-0.7488) in 1984. The fishes collected during the investigation are consisted of 12 species incuding Fugu vermicularis porphyreus, Epinephelus septemfasciatus and Hippocampus coronatus, which are newly found from this area. New establishments of clear water-algal species in this area such as Codium fragile, Hizikia fusiforme, Sargassum fulvellum and Undaria pinnatifida implies that a great change of marine flora have been occurred after the enclosure of the dam. benthic animals are 11 species including Mytilus edulis, Chlamys farreri, Musculus senhausia and Hemicentrotus pulcherrimus, which are newly inhabited in this area. In paricular M. edulis attached abundantly to the shore, and this implies that there was a great deal of ecological change after the enclosure of dam in this area.

凍結前處理가 굴의 冷凍變性 防止에 미치는 效果

趙權玉,趙永濟 釜山水産大學校 1978 釜山水産大學 硏究報告 Vol.18 No.1

凍굴의 凍結變性을 抑制하여 解凍時流出되는 drip量을 減少시키기 위하여 굴을 凍結前에 16種類의 添加物 溶液에 10分間 浸漬處理하여 凍結貯藏한 후 解凍하였을 때의 free drip量, expressible drip量, drip中의 全窒素量, DNA量, VBN量, 그리고 解凍後의 遊離水量 및 加熱 튀김減量 等을 定量하였다. 1) 試料의 個體에 따른 drip流出量은 個體가 작은 것이 큰 것보다 많았으며 處理液의 濃度가 높을수록 drip量이 적었다. 그리고 混合處理區가 單獨處理區 보다 drip流出量이 적었다. 2) drip中의 全窒素量은 大體로 drip流出量에 比例하였으며 混合處理液의 效果가 컸었다. 3) pH는 鹽處理區가 약간 低下하였고 燐酸鹽處理區는 7.0이상이었다. 4) 處理液의 濃度別 및 種類別에 따른 DNA의 變化는 drip流出量과 비슷한 경향을 나타내었다. 5) VBN은 食鹽處理區에서는 增加하였고 燐酸鹽處理區에서는 多少 減少하였으며 drip 流出量과는 반드시 比例하는 것은 아니었다. 6) Sucrose와 燐酸鹽處理區가 遊離水 流出抑制效果가 多少있었다. 7) 加熱튀김減量은 食鹽을 除外한 全處理區 모두 處理液의 濃度가 높을수록 적었다. It is well known that the frozen foodstuffs exude much drip on thawing. This undesirable drip loss is considered to be a measure of quality estimation and of the several factors affecting the drip loss, maintaining freshness of materials may be more important than any others. In the present paper, therefore, the amount of drip and its quality changes were tested in order to find out effect of pretreatment as means of maintaining freshness with several salt solutions on the denaturation of frozen oysters. After shelling, oysters divided into two groups as small and large were washed with fresh water and treated with solutions of sodium chloride, sucrose, poly-mixer(pyro-+tri-;1:1), mixed phosphates(ortho-+meta-+para-; 1:1:1) and these mixture, and then refrigerated at -30℃ by semi-air blast freezer. The total nitrgen, DNA, VBN, released water content and weight loss by cooking as well as the amount of free and expressible drips were also determined during 90 days storae at -25℃. As results of this experiments, the small oysters exude more drip than the large ones. The amount of total drip decreased as the concentration of solutions increased and it is similar to the total nitrogen and DNA in the drip. The amount of VBN increased in the sodium chloride treatment contrary to decrease in the case of phosphate. On the other hand, the pH of drip dropped slightly in treatment with sodium chloride and rose in case of phosphate. The water holding capacity of oysters was counterproportional to the drip loss and the effect of solutions used in treatment. The weight loss by cooking decreased in the all treatments except only one as the concentration of the solutions increased but sodium chloride exhibited adverse effect. It could be concluded from this study that pretreatments with solutions of some salts or sucrose would effective in maintaining the quality of frozen oysters.

玉田 M₃號墳 發掘調査 槪報

崔榮濟,金貞禮,朴升圭 嶺南考古學會 1988 嶺南考古學 Vol.- No.5

玉田古墳의 編年硏究

趙榮濟 嶺南考古學會 1996 嶺南考古學 Vol.- No.18

일 장기 수용시설 노인들의 인지기능장애와 우울증상의 유병율

조맹제,함봉진,이부영,김성윤,신영민,이창인 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 수용시설의 노인들에서 인지기능장애와 우울증상의 유병율을 조사하기 위한 것이다. 방 법 : 한국의 한 수용시설인 꽃동네에 거주하는 60세 이상 노인 169명을 대상으로 한국판 Mini-Mental State Examination(MMSE-K). Short Portable Mental State Questionnaire(SPMSQ), 축약판 Geriatric Depression Scale(SGDS). 그리고 the Center for Epidemiologic Studies Depression (CES-D)을 적용하였다. 결 과 : 전체 169명중 153명(남자=72명, 여자=79명, 그리고 누락=2명)이 검사를 완성하였다. 연령의 범위는 60에서 102세였고, 평균연령은 74.17±8.27(남자71.76±8.14. 여자76.14±7.78)세였다. 교육기간의 범위는 무학에서 16년이었고, 평균교육기간은 3.04±3.93(남자 4.13±4.23, 여자 2.01±3.38)년이었다. 인지기능장애의 유병율은 MMSE-K≤23의 기준으로 56.2%, SPMSQ≤6의 기준으로 26.1% 로 평가되었다. 비교적 분명한 인지기능장애의 기준으로 MMSE-K≤19를 적용하였을 경우 유병율은 25.5%로 이는 SPMSQ≤6을 기준으로 하여 얻은 결과와 유사한 수준이었다. 우울증상의 유병율은 SGDS≥10의 기준으로 19%, CES-D≥25의 기준으로 23.5%로 평가되었다. 비교적 가벼운 우울증상까지 선별하기 위하여 SGDS≥8을 기준으로 할 경우 유병율은 39.2%로 이는 CES-D≥20을 기준으로 하여 얻은 결과와 일치하는 수준이었다. MMSE-K와 SPMSQ, SGDS와 CES-D, MMES-K와CES-D, MMES-K와 SGDS, 그리 고·SPMSQ와 SGDS 사이에 의미있는 상관관계가 관찰되었다. 결 론 : 수용시설 노인들에서 인지기능장애와 우울증상의 유병율은 매우 높은 수준이며, 이러한 결과는 수용시설 노인들에 대한 보다 집중적의 의료서비스가 필요함을 시사한다. Objectives : This study was to investigate the prevalence of cognitive impairment and depressive symptomatology among the long-term institutionalized elderly people. Methods : Mini-Mental State Examination-Korean version(MMSE-K), Short Portable Mental State Questionnaire(SPMSQ), Short version of Geriatric Depression Scale(SGDS), and the Center for Epidemiologic Studies Depression(CES-D) were administerd to 169 elderly residents in an institution, 'Got Dong Rae'. Results : One hundred fifty-three(males=72, females=79, and missing=2) completed the entire examination. Age ranged from 60 to 102 years and the mean of age was 74.17±8.27(males 71.76±8.14, females 76.14±7.78) years. Duration of education ranged from 0 to 16 years and the mean of duration of education was 3.04±3.93(males 4.13±4.23, females 2.01±3.38) years. The prevalence of cognitive impairment was estimated as 56.2% by MMSE-K≤23 and 26.1% by SPMSQ≤6. The prevalence of relatively definite cognitive impairment defined by MMSE-K≤19 was 25.5%, which was similar to the result achieved by using SPMSQ≤6. The prevalence of depressive symptomatology was estimated as 19% by SGDS≥10 and 23.5% by CES-D≥25. In the case of using SGDS≥8 as cutoff-point to screen mild as well as severe depressive symptomatology, the prevalence was 39.2%. It was identical to the result achieved by using CES-D≥20. Significant correlations were observed between MMSE-K and SPMSQ, between SGDS and CES-D, between MMSE-K and CES-D, between MMSE-K and SGDS, and between SPMSQ and SGDS. Conclusion : The prevalencies of cognitive impairment and depressive symptomatology were high in institutionalized elderly people. This results suggests that more intensive mental health care is needed for long-term institutionalized elderly people.

西部慶南 先史文化 地表調査 報告(I) : 河東郡 玉宗面

趙榮濟 부산대사학회 1983 역사와 세계 Vol.- No.7

관상동맥내 스텐트 삽입후 발생한 관상동맥류 2례

제인수,허민영,문치숙,이재호,정수룡,조길현,이영민,김대경,김두일,김동수 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Coronary artery aneurysms are detected with increasing frequency because of the routine use of coronary angiography in the diagnosis and management of coronary artery disease. Although some coronary artery aneurysms are congenital or inflammatory, most are atherosclerotic. The prognosis of atherosclerotic coronary artery aneurysm may depend on the morphology of the aneurysm or the presence of coexisting obstructive disease. Coronary artery aneurysms have been also reported to occur after ballon angioplasty, directional athrectomy, laser angioplasty, and stent implantation with a frequency of 2% to 4%. The long-term outcome of post-intervention coronary artery aneurysm is unknown. We report two cases of the development of a coronary aneurysm 6 months after stent implantation as a treatment of severe obsructive coronary artery disease.