복합재를 이용한 구조물의 균열보수 대책에 관한 연구

李晟熙,韓秀贊,韓秉基,具秉俊 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

Recently, composite materials have widely become available to engineers. This paper briefly explains the applications of these materials and shows how these materials can be used to repair structures with damages such as crack, notch, etc. Before the fatigue test, notched and repaired specimens with thin laminate composite are prepared. Also, test apparatus is designed to perform four point fatigue test of specimens. From the experimental result, it was found that present repair technique could be used to enhance the life of damaged structures.

A Natural Product, Chios Gum Mastic, Induces the Death of HL-60 Cells via Apoptosis and Cell Cycle Arrest

Byung-Chan Koo,Duck-Han Kim,In-Ryoung Kim,Gyoo-Cheon Kim,Hyun-Ho Kwak,Bong-Soo Park KOREAN ACADAMY OF ORAL BIOLOGY 2011 International Journal of Oral Biology Vol.36 No.1

Chios gum mastic (CGM) is produced from Pistiacia lentiscus L var chia, which grows only on Chios Island in Greece. CGM is a kind of resin extracted from the stem and leaves, has been used for many centuries in many Mediterranean countries as a dietary supplement and folk medicine for stomach and duodenal ulcers. CGM is known to induce cell cycle arrest and apoptosis in some cancer cells. This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis following CGM treatment of HL-60 cells. The viability of the HL-60 cells was assessed using the MTT assay. Hoechst staining and DNA electrophoresis were employed to detect HL-60 cells undergoing apoptosis. Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses were also employed. CGM treatment of HL-60 cells was found to result in a dose- and time-dependent decrease in cell viability and apoptotic cell death. Tested HL-60 cells showed a variety of apoptotic manifestations and induced the downregulation of G1 cell cycle-related proteins. Taken collectively, our present findings demonstrate that CGM strongly induces G1 cell cycle arrest via the modulation of cell cycle-related proteins, and also apoptosis via proteasome, mitochondrial and caspase cascades in HL-60 cells. Hence, we provide evidence that a natural product, CGM could be considered as a novel therapeutic for human leukemia.

A Synthetic Chenodeoxycholic Acid Derivative, HS-1200-induced Apoptosis of RBL-2H3 Cells

Byung-Chan Koo,Jeong-Soo Kim,Hyun-Ho Kwak,In-Ryoung Kim,Seung-Eun Lee,Gyoo-Cheon Kim,Hongsuk Suh1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.1

Bile acids and synthetic bile acid derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. Although synthetic chenodeoxycholic acid (CDCA) derivatives have been demonstrated to induce apoptosis of various cancer cells, there is no report on their effect on RBL-2H3 basophilic leukemia cell line to date. Therefore, this study was undertaken to examine in vitro effects of cytotoxicity and growth inhibition, and the molecular mechanism underlying induction of apoptosis in RBL-2H3 cells treated with a synthetic CDCA derivative, HS-1200. The viability and the growth inhibition of RBL-2H3 cells were assessed by MTT assay and clonogenic assay respectively. The Hoechst staining and DNA electrophoresis were conducted to observe RBL-2H3 cells undergoing apoptosis. RBL-2H3 cells were treated with HS-1200, and Western blotting, immunocytochemistry, confocal microscopy, DNA hypoploidy assay, MMP activity and proteasome activity were performed. HS-1200 treatment of RBL-2H3 cells resulted in a time- and does-dependent decrease of cell viability and a does-dependent inhibition of cell growth, and induced apoptotic cell death. Furthermore, HS-1200 treatment result in the alteration of G1 cell cycle-related proteins. And tested RBL-2H3 cells showed several lines of apoptotic manifestation.We presented data indicating that HS-1200 induces apoptois via the proteasome, mitochondria and caspase pathway, and induces the alteration of the G1 cell cycle-related proteins in RBL-2H3 cells. Therefore our data provide the possibility that HS-1200 could be as a novel therapeutic strategy in the allergy treatment.

Pseudomonas aeruginosa로부터 FPLC를 이용한 protease IV의 분리와 그것의 사람구강편평상피암종 세포에 미치는 세포독성효과

Byung-Chan Koo,Mu-Hyung Lee,Hyun-Ho Kwak,Bong-Soo Park,Gyoo-Cheon Kim 대한구강해부학회 2007 대한구강해부학회지 Vol.31 No.1

Oral squamous carcinoma (OSC) cell is responsib1e for the majority of malignancies in the oral cavity. The majority of oral OSC could be treated by a wide surgic외 resection and/or radiation therapy but showed the lowest 5- year survival rate among the major '- cancers. After reports on regression of cancer in humans and animals infected with microbial pathogens date back more than 100 years. much effort has been spent over the years in developing wild-type or attenuated bacterial and purified bacterial proteins for the treatment of cancer. Pseudomonas aeruginosa has extracell띠ar virulence factors such as exotoxin A. exoenzyme S. elastase. protease IV (PIV) and alkaline protease. Pseudomonas aerugino.sa has 아en repoπ.ed to induce apoptosis in epithelial cells. endothelial ceJ1s and macrophage. My laboratory found that the extraceJ1uar products of Pseudo.monas aerugino.있 (EPPA) have a preferential cytotoxic effect on human or외 squamous carcinoma cells compared with human osteosarcoma cells such as HOS and MG63. In order to find the key agent which has such a effect. 1 and collaborators have extracted many kinds of proteins by various methods and studied its cytotoxic effect on OSC cells, 1 obseπed many peaks of proteins from EPPA through FPLC 때d performed to extract 26 kDa PIV from them Because the pattern of PIV peak was distinct from other peaks. it was convenient to extract PIV through FPLC without using the gene c10lÙng‘ When 1 perform protein e1ectrophoresis with extracted PIV. no other bands found except 26 kDa PIV band. suggesting that extracted PIV was high1y pure‘ AJthough the cytotoxic effect of extracted PIV was lower than exotoxin-A and Las B elastase from Pseudo.mo.nas aerulIino.sa. PIV 1ed OSC cells to apoptosis bv means of Hoechst stain

폐경 호르몬요법에서 경구용 Levonorgestrel 투여 1년 후 유방 치밀도에 대한 효과

위찬우 ( Chan Woo Wee ),강민정 ( Min Jung Kang ),서경용 ( Kyoung Yong Seo ),최연현 ( Yeon Hyeon Choe ),민용기 ( Yong Ki Min ),이동윤 ( Dong Yun Lee ),최두석 ( Doo Seok Choi ),윤병구 ( Byung Koo Yoon ) 대한폐경학회 2011 대한폐경학회지 Vol.17 No.1

연구목적: 한국 폐경여성에서 levonorgestrel (LNG)를 이용한 호르몬 치료의 유방 치밀도에 대한 영향을 평가하였다. 연구재료 및 방법: 후향적 연구로서, 이전에 호르몬치료 기왕력이 없는 58명을 대상으로 하였다. 23명에게 estadiol valerate (E)+LNG를 이용해 주기적으로 치료하였고, 25명의 비치료군을 대조군, 10명의 conjugated equine estrogen (CEE)+medroxyprogesterone acetate (MPA) 치료군을 양성 대조군으로 두었다. 이후 기저치와 1년 후 유방촬영을 시행하여 치밀도를 비교하였다. 치료 전후의 X선 유방촬영술상 유방 치밀도의 변화는 J-image 프로그램을 이용하여 평가하였다. 자료는 chi-square 분석 및 Fisher`s exact 분석법, 그리고 nalysis of variance (ANOVA)을 이용하여 분석하였다. 결 과: 세 군 간의 기저 임상적 특성에 차이는 없었다. 1년의 호르몬요법 후, breast imaging reporting and data system (BI-RADS) 기준에 따른 변화를 보았을 때 CEE+MPA 사용군 (77.8% [7/9])이, E + LNG 사용군 (12.5% [2/16])보다 유의하게 증가하였으나 (P=0.001), E+LNG 사용군과 비치료군 (5.3% [1/19])사이에 유의한 차이는 없었다 (P=0.446). 치밀도가 3단계인 환자만을 대상으로 한 분석에서도 E+LNG 사용군은 비치료군과 유방 치밀도 변화에 차이가 없었다. J-image 프로그램을 이용한 분석 결과도 유사한 결과를 보였다. 또한 회귀 분석 결과, 기저 치밀도와 호르몬요법후 치밀 면적의 변화 사이에는 상관 관계가 나타나지 않았다. 결론: 한국 폐경 여성에서 LNG를 이용한 호르몬요법은 유방 치밀도에 영향을 미치지 않는다. 향후 유방암 위험에 대한 추가 연구가 필요하다고 사료된다. Objectives: We evaluated changes in mammographic density (MMGD) after hormone therapy (HT) using levonorgestrel (LNG) in Korean postmenopausal women. Methods: In this retrospective study, 58 healthy postmenopausal women with no history of prior HT were included. Twenty-three women were given a cyclic regimen of estradiol valerate (E) 2 mg/day+LNG 0.075 mg/day and 25 untreated women served as a negative control and 10 women treated with continuous combined regimen of conjugated equine estrogen (CEE) 0.625 mg/day+medroxyprogesterone acetate (MPA) 2.5 mg/day as a positive control. Mammography was obtained at baseline and 1 year later, and MMGD was assessed. In addition, the change in the proportion of the dense area after HT was assessed using the J-image program. Data were analyzed with analysis of variance and chi-square or Fisher`s exact test. Results: There were no significant differences in baseline characteristics among three groups. After 1 year of HT, increase of BI-RADS grade in CEE+MPA group (7/9, 77.8%) was higher than in E+LNG group (2/16, 12.5%; P=0.001). But there was no difference between untreated group (1/19, 5.3%) and E+LNG group (2/16, 12.5%; P=0.446). In subgroup analysis of women with BI-RADS grade 3 at baseline, no significant difference. Changes in the proportion of dense areas assessed by the J-image program showed similar results. Regression analysis revealed that there was no correlation between baseline dense area and changes after HT in E+LNG group. Conclusion: HT using LNG did not influence MMGD in Korean postmenopausal women. Further study on breast cancer risk may be needed. (J Korean Soc Menopause 2011;17:27-33)

Direct Alcohol-Fueled Low-Temperature Solid Oxide Fuel Cells: A Review

Yang, Byung Chan,Koo, Junmo,Shin, Jeong Woo,Go, Dohyun,Shim, Joon Hyung,An, Jihwan JOHN WILEY AND SONS LTD 2019 Energy technology Vol.7 No.1

급성 심근경색증 환자에서 조기에 적절한 재관류 요법을 시행받지못한 요인

유병수(Byung Su Yoo),윤정한(Jung Han Yoon),박금수(Keum Soo Park),여경구(Kyung Koo Yoh),조윤경(Yun Kyung Cho),안승찬(Seung Chan Ahn),이용규(Yong Kyu Lee),송광선(Kwang Seon Song),최경훈(Kyung Hoon Choe),이부수(Boo Soo Lee),황성오(Sung 대한내과학회 1995 대한내과학회지 Vol.48 No.6

N/A Objectives: Early application of reperfusion therapy such as thrombolytic agent or direct PTCA is utmost important to amximize the efficacy of reperfusion therapy in patients with aute myocardial infarction. However, early adequate reperfusion thearpy was given only in 15% to 36% pf patients with acute myocardial infarction and substantial patients were not benefited from reperfusion therapy, Therefore, we performed these study to evaluate the reasons for which patients with acute myocardial infarction cannot receive an adequate reperfusion therapy. Methods: We analyzed the initial electrocardiogram and various time delays from chest pain onset time, first hospital arrival time, transfer time, ER arrival time, and door to reperfusion time in 138 patients with acute myocardial infarction from Jan. 1991 to Oct. 1993 admitted to Wonju Christian Hospital. Patients were grouped as reperfusion group(n=55) who had reperfusion therapy such as thrombolytic trial or direct PTCA and conservative grou who had not received reperfusion therapy(n= 83). Results: 1) Eighty-three cases(60.1%) did not received an adequate reperfusion therapy. 2) Hospital arrival time of patients was 237±162min and 786±615min in reperfusion and conservative group(p<0.05) respectively. Patient time delay was 103±98min and 439±511min, first hospital time delay, 93±78min and 333±482min, and transfer time, 81±59min and 105±64min in reperfusion and conservative group respectively(p<0.05). Only 4.8% of patients was transferred from first hospital after reperfusion therapy. 3) Patient time delay was the most common reason of not receiving reperfusion therapy, and time delay and lack of adequate reperfusion therapy at the first hospital inspite of early arrival were the second place. Other reasons were contraindication to lytic therapy(4 cases), definite Q wave MI(11 cases), inconclusive EKG(4 cases), and delay in therapy(4 cases). Conclusion: The most common reason of not receiving reperfusion therapy was patient delay in seeking medical care(45.8%) and time delay at first hospital in 22 cases(26.5%). To maximize the effectiveness of reperfusion therapy, it is important to shorten hospital arrival time delay and widespread use of reperfusion therapy at first hospital is recommeded.

논문 : 횡성지역 다양한 지질환경에서 지하수 중 우라늄 및 라돈-222 산출특성

정찬호 ( Chan Ho Jeong ),양재하 ( Jae Ha Yang ),이유진 ( Yu Jin Lee ),이용천 ( Yong Cheon Lee ),최현영 ( Hyeon Young Choi ),김문수 ( Moon Su Kim ),김현구 ( Hyun Koo Kim ),김태승 ( Tae Seong Kim ),조병욱 ( Byung Uk Jo ) 대한지질공학회 2015 지질공학 Vol.25 No.4

지하수내 자연방사성물질인 우라늄과 라돈-222의 산출과 지질특성과의 연관성을 알아보기 위해 단층대를 포함한 화강암, 편마암, 복운모 화강암 등 다양한 지질이 분포하는 횡성지역을 연구대상지역으로 하였다. 이 연구를 위하여 지하수 시료 38점, 지표수 시료 4점을 채취하여 화학성분 분석, 우라늄과 라돈-222의 함량을 분석하였다. 1차 분석결과를 바탕으로 우라늄과 라돈-222의 함량이 미국 EPA 권고기준을 초과한 지하수 16점에 대해서는 2차 분석을 실시하였다. 지하수내 자연방사성물질 산출과 지질과의 상관성을 알아보기 위하여 33개지점에 대한 지표방사능 세기를 측정하였다. 지하수내 우라늄의 농도는 0.02~49.3 μg/L의 범위를, 라돈-222의 농도는 20~906 Bq/L 범위로 미국 EPA 권고기준치인 30 μg/L와 148 Bq/L을 초과한 시료는 각각 4점과 35점이다. 지하수의 화학적 특성은 Ca(Na)-HCO3 유형에서Ca(Na)-NO3(HCO3+Cl) 유형 범위까지 분포한다. pH는 5.71~8.66의 범위로 중간 값은 중성 또는 약알카리성의 특성을 보였다. 고함량 우라늄 및 라돈-222의 산출은 편마암-화강암 지질경계부과 화강암내에서 주로 확인되었으며, 우라늄과 라돈의 산출에 대한 상관관계는 뚜렷하지 않다. 불활성 기체인 라돈은 암석내 기원지로부터 주변부에 발달된 단열을 따라서 확산되어 순환하는 지하수에 용해되는 것으로 보이며, 지하수의 유동과 화학성분과의 상관성은 찾아보기 어렵다. 지하수내 우라늄 용해에 유리한 조건은 중성 또는 약알칼리성 환경과 산화 환경이면서 높은 중탄산 함량의 지화학적 조건에서 주로 우라닐 또는 우라닐탄산염 형태로 존재하는 것으로 해석된다. Groundwaters in granite, gneiss, and two-mica granite formations, including faults, in the Hoengseong area are examined to determine the relationship between their uranium and radon-222 contents and rock types. The chemical compositions of 38 groundwater samples and four surface water samples collected in the study area were analyzed. Sixteen of the samples showing high uranium and radon-222 contents were repeatedly analyzed. Surface radioactivities were measured at 30 points. The uranium and radon-222 concentrations in the groundwater samples were in the ranges of 0.02-49.3 μg/L and 20-906 Bq/L, respectively. Four samples for uranium and 35 samples for radon had concentrations exceeding the alternative maximum contaminant level of the US EPA. The chemical compositions of groundwaters indicated Ca(Na)-HCO3 and Ca(Na)-NO3(HCO3+Cl) types. The pH values ranged from 5.71 to 8.66. High uranium and radon-222 contents in the groundwaters occurred mainly at the boundary between granite and gneiss, and in the granite area. The occurrence of uranium did not show any distinct relationship to that of radon-222. The radon-222, an inert gas, appeared to be dissolved in the groundwater of the aquifer after wide diffusion along rock fractures, having been derived from the decay of uranium in underground rocks. The results in this study indicate that groundwater of neutral or weakly alkaline pH, under oxidizing conditions and with a high bicarbonate content is favorable for the dissolution of uranium and uranium complexes such as uranyl or uranyl-carbonate.

다양한 지질환경에서 지하수의 수리화학 및 자연방사성물질 산출특성

정찬호 ( Chan Ho Jeong ),이유진 ( Yu Jin Lee ),이용천 ( Yong Cheon Lee ),김문수 ( Moon Su Kim ),김현구 ( Hyun Koo Kim ),김태승 ( Tae Seong Kim ),조병욱 ( Byung Uk Jo ),최현영 ( Hyeon Young Choi ) 대한지질공학회 2016 지질공학 Vol.26 No.4

화강암, 화강편마암, 변성퇴적암류와 같이 다양한 지질환경에서 지하수내 자연방사성물질인 우라늄과 Rn-222의 산출특성에 대한 지하수의 수리화학적 영향, 지질과의 상관성, 단층대의 영향 등에 대해서 알아보고자 하였다. 이 연구를 위하여 영동지역을 대상으로 2차례에 걸쳐 지하수 49점, 지표수 4점을 채취하였다. 지하수내 우라늄과 지표 암석과의 상관성을 알아보기 위해 감마스펙트로메트리를 이용하여 40지점에서 지표방사능을 측정하였다. 지하수 화학적 유형은 Ca-HCO₃, Na-HCO₃,, Ca-HCO₃(SO₄+NO₃)등 3가지 유형을 보인다. 환경부 권고치인 우라늄 30 μg/L를 초과하는 지하수는 총 49지점 중 2점이며, Rn-222의 경우 미국 EPA 기준치인 148 Bq/L를 초과하는 지하수는 총 40지점중 11점이다. 초과하는 지하수는 주로 화강편마암과 흑운모편마암 지질과 지질경계부에 분포한다. 지표방사능 세기와 지하수내 우라늄함량과는 뚜렷한 상관관계를 보여지 않는다. 아울러 N45°E 방향의 주향이동단층인 영동단층은 82°의 고경도로 상반에 해당되는 화강암 및 화강편마암지역에서 고함량의 우라늄과 Rn-222가 산출되며, 하반에 해당되는 퇴적암지역에는 고함량의 지하수가 확인되지 않는데, 이와 같은 뚜렷한 차이는 지질의 영향과 더불어 단층대가 방사성물질의 이동 및 확산을 차단시키는 역할에도 원인이 있을 것으로 추정된다. The purpose of this study is to analyze the relationship of hydrochemistry, geology, fault with occurrence of uranium and radon-222 from the groundwater in the Yeongdong area. In this study, 49 groundwater and 4 surface water samples collected in the study area were collected on two separate occasions. The surface radioactivities were measured at 40 points to know the relationship between the occurrence of uranium in groundwater and surface geology. The chemical composition of groundwater showed three types : Ca-HCO₃, Na-HCO₃ and Ca-HCO₃(SO₄, NO₃). Two groundwater of 49 samples exceeded the maximum contaminant levels of uranium, 30 μg/L, proposed by the Ministry of Environment of Korea and 11 groundwater of 40 samples for Rn-222 concentrations exceeded the 148 Bq/L maximum contaminant level of US EPA. Most of unsuitable groundwater are located in the geological boundary related with the biotite gneiss and the surface radioactivities of rock samples showed no relationship with groundwater geochemical constituents. The strike-slip fault, Youngdong fault, is N45˚E direction and the high concentrations of uranium in upper part of fault, consisted of granite and granitic gneiss are detected but in lower part, consisted of metamorphic sedimentary rock are not detected. It suggests that the natural radioactive concentrations are related with the geologic characteristics and the migration and diffusion of natural radioactive materials are affected by the fault.

미만성 신피질 석회화증 : 1예보고

고병희,조온구,함창곡,박찬현,최윤영 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

Nephrocalcinosis refers to the pathologic deposition of calcium salts in the renal parenchyma. According to its location, it is classified medullary and cortical nephrocalcinosis. The less common cortical nephrocalcinosis results mainly form acute cortical necorsis and chronic glomerulonephritis. We report a case of increased cortical echogenicity on sonograms and increased cortical attenuation on computed tomograms by diffuse cortical nephrocalcinosis secondary to chronic glomerulonephritis.