Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells

Xiao-hui Liao,Rui-ting Long,Jun-bo Peng,Li-li Huang,Gui-ping Jiang,Yue-juan Liao,Hang Sun,Yu-dong Hu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

Preparation and properties of the green-emitting phosphors NaCa0.98-xMgxPO4:

Bo Yue,Juan Gu,Guangfu Yin,Zhongbing Huang,Xiaoming Liao,Yadong Yao,Yunqing Kang,Panli You 한국물리학회 2010 Current Applied Physics Vol.10 No.4

In this study, a series of the green-emitting phosphors NaCa0.98-xMgxPO4:Eu2þ 0:02 was prepared through high temperature solid-state reaction. The structure and spectroscopic properties of these samples were characterized by XRD, FT-IR, reflectance spectra and photoluminescence (PL) spectra, respectively. The effect of Mg2+ co-doping concentration on the PL intensity of as-prepared samples was also investigated. The enhanced intensities of excitation and emission band of Eu2+ and a slight red-shift of the emission peak were observed due to Mg2+ co-doping. When the x value is 0.08, the phosphors possessed the maximum emission intensity monitored at 360 nm and the emission spectrum shows a single band centered at about 500 nm. The obtained phosphors had strong absorption in near-UV zone (350–400 nm), which can match the emission of near-UV light-emitting diodes. The prepared material is potential green phosphors for white LEDs.

STUDY ON TRESS-STRAIN RELATIONSHIP ON LOESS BASED ON TWIN SHEAR UNIFIED DAMAGE CONSTITUTIVE MODEL

BO HAN,HANGZHOU LI,HONG-JIAN LIAO,ZHENGHUA XIAO 한국소성가공학회 2008 한국소성가공학회 컨퍼런스 Vol.2008 No.10

DC component Mitigation Utilizing BP-PI and Repetitive Control in Transformless Grid-Connected Inverters

Bo Long,Yong Liao,YuFei Dai,LiJun Huang,Xin Lu,Yong Chen,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Transformerless grid-connected inverters, due to their advantages in high efficiency, small volume and light weight, gain more research and interest in recent years. Due to the asymmetrical driving signal in pulse-width-modulation (PWM) caused by time-delay, zero-drift of the current sensors and imparities of the power transistors, etc. Output of the grid current contains dc component. As a result, power quality of the grid is degraded. In this paper, a dc component suppression scheme with recursive integral PI controller are introduced, according to the change tendency of tracking error, the coefficients of PI controller are regulated by BP neural network in real time, and the global optimization requirements are met. Sliding-Window-Double-Iteration-Method (SWDIM) is utilized for fast dc current extraction. Simulation results from a 2-kW three-phase grid-connected inverter confirm the effectiveness of the proposed scheme.

Numerator-Denominator Model Based H<SUB>∞</SUB> Robust Control for LCL Filter-Based Grid-Connected Inverters under Weak Grid Conditions

Bo Long,Chu Wang,ZiLin Zhu,Xin Lu,LiJun Huang,YuFei Dai,Yong Liao,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In the grid-connected inverter controller system, conventional PI or PR controller decrease their lowfrequency gain and bandwidth to maintain the stability of the system. However, this degrades the disturbance rejection and current tracking capability. To solve this problem, this paper gives an H∞ robust control scheme based on Numerator Denominator Model method, which has the advantages of simplified current controller design procedure, can place the poles of closed-loop system at the desired place. By properly selecting the weight function, the proposed control scheme can not only achieve good robustness in disturbance rejection capability, but also gives a straight forward to design the dynamic performance. Simulation and experimental comparison results between PR and H∞ controller is demonstrated. The results prove that the proposed control scheme shows obviously improvements on the robustness of inverter and meanwhile does not lose advantages on the dynamic performance.

The Control of Collaboration for Comprehensive Transportation Corridor

LIAO Yong,ZHANG Yan,CHEN Bo-li,LV Ming 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Regional comprehensive corridor takes on the characteristics of heavy transport demand and supply, and consists of many travel modes; meanwhile, competition is severe among different trip modes. In order to avoid vicious competition so that transportation market is disturbed, multi-objective and nonlinear programming model is built up, which is based on the maximization of unity of traveler. The objective of model is minimization of distance between practical capacity coefficient and ideal capacity coefficient. Multi-objective is transferred into single objective by TOPSIS method before model is solved. The transformed objective function is multi-peak function, so niche GA is introduced into solving the problem to obtain all of optimal solution. K-means clustering is adapted to classify the set of optimal solution. The centre of gravity of each cluster is taken as value of decision variables. At last a numeric example demonstrates the validity and feasibility.

Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703

Xiaolin Liao,Yongjie Lu,Junbao Yang,Tao Kuang,Lilin Jiang,Yanjun Wang,Huiqun Kang,Bo Jiang,Xiaoli Zhou,Sheng He 한국유전학회 2017 Genes & Genomics Vol.39 No.10

Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of sitespecific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.

In vitro evaluation of 9-(2-phosphonylmethoxyethyl)adenine ester analogues, a series of anti-HBV structures with improved plasma stability and liver release

Sha Liao,Shi-Yong Fan,Qin Liu,Chang-Kun L,Jia Chen,Jing-Lai Li,Zhi-Wei Zhang,Zhen-Qing Zhang,Bo-Hua Zhong,Jian-Wei Xie 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.11

Chronic hepatitis B virus (HBV) infection maylead to liver cirrhosis and hepatocellular carcinoma, butfew drugs are available for its treatment. Acyclic nucleosidephosphonates (ANPs) have remarkable antivirusactivities but are not easily absorbed from the gastrointestinaltract and accumulate in the kidneys, resulting innephrotoxicity. Therefore, there is a need to find effectiveliver site-specific prodrugs. The dipivaloyloxymethyl esterof 9-(2-phosphonylmethoxyethyl)adenine (PMEA)—adefovirdipivoxil (ADV)—is a first-line therapy drug forchronic hepatitis B with a low therapeutic index because ofrenal toxicity and low hepatic uptake. In this study, a seriesof PMEA derivatives were synthesized to enhance plasmastability and liver release. The metabolic stability of ADV(Chemical I) and its two analogues (Chemicals II and III)was evaluated in rat plasma and liver homogenate in vitro. An ion-pair reverse-phase HPLC–UV method and a hybridion trap and high-resolution time-of-flight mass spectrometry(LC-IT-TOF-MS) were used to evaluate the degradationrate of the analogues and to identify their intermediatemetabolites, respectively. Chemicals I and II were hydrolyzedby cleavage of the C–O bond to give monoesters. Sufficient enzymatic activation in the liver homogenatethrough a relatively simple metabolic pathway, in additionto a favorable stability profile in rat plasma, made ChemicalII an optimal candidate. Next, six analogues based onthe structure of Chemical II were synthesized and evaluatedin plasma and liver homogenate. Compared toChemical II, these compounds generated less active PMEAlevels in rat liver homogenate. Therefore, chemical modificationof Chemical II may lead to new promising PMEAderivatives with enhanced plasma stability and liveractivation.

Characterization and expression analysis of inositolphosphorylceramide synthase family genes in rice (Oryza sativa L.)

Pengfei Liao,Jinqiu Huang,Puguo Tong,Wang Nie,Xin Yan,Yingmei Feng,Hui Peng,Xiaojue Peng,Shao-Bo Li 한국유전학회 2017 Genes & Genomics Vol.39 No.5

Genes encoding inositolphosphorylceramide synthase (IPCS), which play essential roles in sphingolipid biosynthesis, have been known to be widely distributed in plants. However, reports on identification, expression, and function analysis of plant IPCS family genes are very limited. In this study, a total of three OsIPCS genes were identified in the rice database, and their full-length coding regions were amplified by RT-PCR. These genes had 12–13 exons, and they encoded proteins of 313–326 amino acids with molecular mass of 35.8–37.6 kDa and isoelectric point of 10.99–12.61. Importantly, two typical and conserved motifs (D3 and D4) in plants were found in all the three putative OsIPCS proteins, indicating high similarity of OsIPCSs to the other plant IPCS proteins, which were confirmed by phylogenetic analysis. The transcripts of OsIPCS genes appeared in different rice organs including seedling roots, stems and leaves, and young panicles, but each gene showed a unique organ-specific expression pattern. Meanwhile, we found that all the three OsIPCS genes showed responses to stresses like drought, cold and salt, but their expression patterns were also to some extent different under stress treatments. Especially, under cold stress, OsIPCS1 and OsIPCS2 were significantly up-regulated in roots and stems but down-regulated in leaves, while OsIPCS3 was significantly up-regulated in all the measured organs. Actually, putative abiotic-stress regulatory elements were identified in the promoters of these genes. Our data suggest that OsIPCS genes should play important roles during rice growth and adversity adaptation.

An Adaptive Learning System based on Cloud Computing : Implementation and Evaluation of BDS

Yi Liao,Lei Huang,Hang Zhou,Bo Li 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.8

In this paper, we report the design and implementation of an adaptive individualized e-learning environment based on cloud computing named BDS (Beijing Digital School). With its five-layer architecture and its use of cloud computing technology, BDS possesses the characteristics of both a massive access system and an adaptive e-learning environment. This paper focuses on investigating three distributed solutions proposed for load balancing: an active clustering algorithm, a random algorithm and a honey bee foraging algorithm. An experiment comparing 2 groups of students was conducted to evaluate the impact of the proposed environment on learning performance, and the results were analyzed to determine the most effective strategy.