Fast and robust misalignment correction of Fourier ptychographic microscopy for full field of view reconstruction

Zhou, Ao,Wang, Wei,Chen, Ni,Lam, Edmund Y.,Lee, Byoungho,Situ, Guohaiz The Optical Society 2018 Optics express Vol.26 No.18

Host factor TNK2 is required for influenza virus infection

Zhou Ao,Zhang Wenhua,Wang Baoxin 한국유전학회 2023 Genes & Genomics Vol.45 No.6

Background Host factors are required for Influenza virus infection and have great potential to become antiviral target. Objective Here we demonstrate the role of TNK2 in influenza virus infection. CRISPR/Cas9 induced TNK2 deletion in A549 cells. Methods CRISPR/Cas9-mediated deletion of TNK2. Western blotting and qPCR was used to measure the expression of TNK2 and other proteins. Results CRISPR/Cas9-mediated deletion of TNK2 decreased the replication of influenza virus and significantly inhibited the ex-pression of viral proteins and TNK2 inhibitors (XMD8-87 and AIM-100) reduced the expression of influenza M2, while over-expression of TNK2 weakened the resistance of TNK2-knockout cells to influenza virus infection. Furthermore, a decrease of nuclear import of IAV in the infected TNK2 mutant cells was observed in 3 h post-infection. Interestingly, TNK2 deletion enhanced the colocalization of LC3 with autophagic receptor p62 and led to the attenuation of influenza virus-caused accumulation of autophagosomes in TNK2 mutant cells. Further, confocal microscopy visualization result showed that influenza viral matrix 2 (M2) was colocalized with Lamp1 in the infected TNK2 mutant cells in early infection, while almost no colocalization between M2 and Lamp1 was observed in IAV-infected wild-type cells. Moreover, TNK2 depletion also affected the trafficking of early endosome and the movement of influenza viral NP and M2. Conclusion Our results identified TNK2 as a critical host factor for influenza viral M2 protein trafficking, suggesting that TNK2 will be an attractive target for the development of antivirals therapeutics.

Effects of Fermented Garlic Powder on Production Performance, Egg Quality, Blood Profiles and Fatty Acids Composition of Egg Yolk in Laying Hens

Ao, X.,Yoo, J.S.,Lee, J.H.,Jang, H.D.,Wang, J.P.,Zhou, T.X.,Kim, I.H. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.6

The effects of fermented garlic powder on production performance, egg quality, blood profiles and fatty acid composition of egg yolk in laying hens were studied in a 35-d experiment. Two-hundred and forty (ISA brown) layers aged 41 weeks were randomly allocated into the following four treatments: i) CON (basal diet); ii) G1 (CON+fermented garlic powder 1.0%); iii) G2 (CON+fermented garlic powder 2.0%) and iv) G3 (CON+fermented garlic powder 3.0%). There were no differences (p>0.05) among treatments in egg production, egg weight, eggshell breaking strength and eggshell thickness throughout the whole experimental period. However, yolk height was increased significantly (p<0.05) by the addition of fermented garlic powder during the 5th week while yolk color was greater (p<0.05) in G2 and G3 than in CON and G1 in the 5th week. Compared with CON, Haugh unit was increased (p<0.05) in response to fermented garlic powder treatments during the 5th week. No significant effects on total protein, albumin and IgG were observed in response to any of the treatments over the experimental period (p>0.05). There was a significant (p<0.05) reduction in plasma cholesterol concentration when the dietary level of fermented garlic powder was increased from 0.0 to 3.0%. The levels of saturated fatty acids (SFA) were significantly decreased (p<0.05) in response to G2 and G3 while monounsaturated fatty acids (MUFA) were higher (p<0.05) in G2 and G3 treatment groups than in CON and G1. Compared with other treatments, polyunsaturated fatty acids (PUFA) and PUFA:SFA ratio were higher (p<0.05) in G3. In conclusion, this study demonstrated that addition of fermented garlic powder reduced plasma cholesterol concentration and did not cause adverse effects on production performance. Moreover, addition of 3.0% garlic powder decreased SFA but increased PUFA and PUFA:SFA ratio in egg yolk.

Influence of Fermented Red Ginseng Extract on Broilers and Laying Hens

Ao, X.,Zhou, T.X.,Kim, H.J.,Hong, S.M.,Kim, I.H. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7

The aim of this study was to evaluate the potential of fermented red ginseng extract (FRGE) as feed additive in broilers and laying hens. In broilers, 480 Arbor Acre male broilers were randomly allotted to 4 treatments with 6 replications per treatment and 20 chicks per pen. The experiment lasted 5 weeks and dietary treatments were as follows: i) CON, basal diet; ii) FRGE1, basal diet+1 g/kg fermented red ginseng extract; iii) FRGE2, basal diet+2 g/kg fermented red ginseng extract and iv) FRGE3 basal diet+4 g/kg fermented red ginseng extract. Throughout the experiment, no effects were observed (p>0.05) in performance in response to FRGE. At the end of the experiment, FRGE administration improved (p<0.05) the lymphocyte level compared with CON. The relative weight of bursa of fabricius and spleen were increased (p<0.05) by the inclusion of FRGE3. Besides, redness ($a^*$) value for the breast meat was higher (p<0.05) in FRGE1 and FRGE3 treatments than that in CON. In laying hens, 240 ISA brown layers at 35 weeks of age were used in this 8-week trial. Dietary treatments were the same as in the broilers trial with 10 replicates per treatment and 6 layers per replicate. During the entire experiment, there were no significant differences (p>0.05) in performance or egg quality among all the treatments. However, the layers fed diets supplemented with FRGE had higher lymphocyte level (p<0.05) compared with those fed CON. In conclusion, the dietary supplementation with FRGE did not influence performance but improved the lymphocyte level in both broilers and laying hens.

Influence of Injection System Parameters on Combustion Noise of a Small Power Non-Road Diesel Engine

Zhou Xinfeng,Ma Zhihao,Liu Yuna,Li Ao 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.5

Combustion noise accounts for a large proportion of diesel engine noise. On the premise of not increasing the cost of diesel engine, the injection system parameters such as dynamic fuel supply advance angle and needle valve opening pressure are optimized, and the influence of the above parameters on the in-cylinder pressure, rate of pressure rise, in-cylinder pressure level (CPL) spectrum and high frequency pressure oscillation is analyzed. The study shows that reducing the dynamic fuel supply advance angle can effectively reduce the combustion noise, and appropriately increasing the needle valve opening pressure can also reduce the combustion noise. After the optimization of injection parameters, the combustion noise of the diesel engine with an electronically controlled VE pump-tube-nozzle injection system is significantly reduced compared with the prototype engine, and can reach the same level of combustion noise as the diesel engine with the common rail system without deterioration of fuel economy and emission performance. The experimental study can provide technical reference for diesel engines in the selection of injection system parameters and the optimization of combustion noise in the improvement stage.

Preparation and property evaluation of a temperature-resistant Zr-crosslinked fracturing fluid

Ming Zhou,Jinfeng Zhang,Zhonghua Zuo,Mao Liao,Peng’ao Peng 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-

Compared with the traditional water-based fracturingfluid thickener guanidine gum, the price ofsynthetic polymer is relatively low, and the temperature resistance, shear resistance and viscoelasticityof the formed fracturingfluid are better. Therefore, the fracturingfluid formed by the crosslinking ofHPAM and organic metal crosslinker will become one of the hot spots in the research of water-basedfracturingfluid. In this paper, zirconium oxychloride, acetic acid and triethanolamine were used as rawmaterials to prepare organic Zr-crosslinked agent. The polymer gel was formed by crosslinking theorganic Zr-crosslinked agent with the acrylamide copolymer KY-5S solution, and the viscosity of the gelwith different concentrations was investigated. The performance of the fracturingfluid was evaluatedthrough laboratory experiments. The results showed that the viscosity of the fracturingfluid remainedabove 100 mPa s at the shear rate of 170 s 1 and after 2 h of shear at 140 C. When the temperature was20 C, the shear stress was 0.1 Pa, and the frequency f varied from 0.1 to 10 Hz, the elastic modulus offracturingfluid was at least 11.6 Pa, and the viscous modulus was at least 8.54 Pa. At the temperature of90 C, when the addition of glue breaker was 0.01 wt%, the longest breaking time was 3 h, and themaximum viscosity of breaking liquid was 4.05 mPa s. The damage rate of core permeability of gluebreaker was about 13%. When the temperature was 25 C and the shear rate was 0–170 s-1, the viscosity offracturingfluid remained above 200 mPa s.

Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing

Ming Yao,Jiali Zhou,Yicheng Zhu,Yinxin Zhang,Xia Lv,Ruixue Sun,Ao Shen,Haitao Ren,Liying Cui,Hongzhi Guan,Honglong Wu 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.4

Background and Purpose Encephalitis caused by Listeria monocytogenes (L. monocyto¬genes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal flu¬id (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens. Methods This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis. Results Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocy¬togenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and se¬quenced reads corresponding to L. monocytogenes in all three cases. Conclusions This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.

D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation

Bo Qiao,Bin He,Ao Zhou,Yuling Li,Xiaojun Chen,Qiming Yang,Beike Chen,Bo Qiao,Dianming Jiang 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.2

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (DRADA16- RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD). METHODS: Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo. RESULTS: Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/ D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/ D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity. CONCLUSION: This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.

Microstructure and cutting performance of CrTiAlN coating for high-speed dry milling

LU, Li,WANG, Qi-min,CHEN, Bing-zhou,AO, Yong-cui,YU, Dong-hai,WANG, Cheng-yong,WU, Shang-hua,KIM, Kwang Ho Elsevier 2014 Transactions of Nonferrous Metals Society of China Vol.24 No.6

<P>Using a closed field unbalanced magnetron sputtering system, the cemented carbide end mills were coated with a CrTiAIN hard coating, which consisted of a Cr adhesive layer, a CrN interlayer and a CrTiAIN top layer. The microstructure and mechanical properties of the coating were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), micro indentation and scratch test. The cutting performance of the coated end mills were conducted by high-speed dry milling hardened steel (P20, HRC 45). The results indicates that the coating is composed of (Cr, Ti, Al)N columnar grains with nanolayers. The coating exhibits good adhesion to cemented carbide substrate and high microhardness of around 30 GPa. The coated end mills show significant improvement on tool life and much lower cutting force as compared to the uncoated ones. And the related mechanisms were discussed.</P>

Classification of soybeans from different habitats based on metabolomic–transcriptomic integration

Wang Jinghui,Zheng Qiyou,Wang Chenxu,Zhou Ao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Soybeans are a significant agricultural product in China, with certain geographical locations often yielding higher quality, and thus more expensive, soybean crops. In this study, metabolomics and transcriptomics analyses were con ducted on soybean samples from nine regions in Heilongjiang and Liaoning Provinces using untargeted liquid chro matography–mass spectrometry (LC–MS) and Illumina sequencing technologies. The primary objective was to devise an effective and unbiased method for determining the geographical origin of each soybean variety to mitigate potential fraudulent practices. Through multidimensional and unidimensional analyses, successful identification of dif ferentially expressed metabolites (DEMs) and differentially expressed genes (DEGs) was achieved, yielding statisti cally significant outcomes. Integration of the metabolomics and transcriptomics datasets facilitated the construction of a correlation network model capable of distinguishing soybeans originating from different geographical locations, leading to the identification of significant biomarkers exemplifying noteworthy distinctions. To validate the feasibil ity of this method in practical applications, partial least squares discriminant analysis was employed to differentiate soybean samples from the nine regions. The results convincingly showcased the applicability and reliability of this approach in accurately pinpointing the geographical origin of soybeans. Distinguishing itself from prior research in soybean traceability, this study incorporates an integrated analysis of metabolomics and transcriptomics data, thereby unveiling biomarkers that offer a more precise differentiation of soybean traits across distinct regions, thereby bridging a critical research gap within the soybean traceability domain. This innovative dual-data integration analysis methodology is poised to enhance the accuracy of soybean traceability tools and lay a new foundation for future agricultural product identification research.