Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus

Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.

Ziwuliuzhu Acupuncture Modulates Clock mRNA, Bmal1 mRNA and Melatonin in Insomnia Rats

Huang Ao,Xiao Gefang,Chen Yiliu,Hu Zuying,Lee Pin-Hsuan,Huang Yusen,Zhuang Zifeng,Zhang Yuling,Qing Peng,Zhao Canghuan 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.3

Background: In clinics, Ziwuliuzhu acupuncture is widely considered an effective method of treating insomnia; however, there is currently limited information available regarding its possible mechanisms. Although the method of Ziwuliuzhu acupuncture possesses a unique rhythmic pattern. Objectives: In this study, we have creatively combined the traditional Chinese medicine of Ziwuliuzhu with a modern biological rhythm to investigate the internal mechanism of insomnia. Methods: Pathological tissue from the hypothalamus was analyzed using hematoxylin– eosin staining. The level of TNF (tumor necrosis factor)-α in the SCN (suprachiasmatic nucleus) area of the hypothalamus was detected in situ using the TUNEL fluorescence staining assay. The concentration of hypothalamic melatonin was detected using the enzyme-linked immunosorbent assay (ELISA). The mRNA expression of Clock and Bmal1 was measured using RT-qPCR. Results: In the Ziwuliuzhu acupuncture groups, the structural damage in the hypothalamic neurons was alleviated compared to the model group and the expression of inflammatory factors was reduced. The mRNA expression levels of Clock and Bmal1 were significantly increased (p < 0.05). The concentration of melatonin was significantly increased (p < 0.001). Although there were no significant differences between the treatment groups (diazepam group, Nazi group, Najia group, and routine group) (p > 0.05). Conclusion: Ziwuliuzhu acupuncture alleviated neuronal damage and modulated the inflammatory reaction in the hypothalamus of rats with insomnia. Moreover, Ziwuliuzhu acupuncture increased the expression levels of Clock and Bmal1 mRNA, and MT content. This study has potentially highlighted one of

Identification and comparative profiling of microRNAs in wild-type Xanthoceras sorbifolia and its double flower mutant

Yan Ao,Yanwei Wang,Lei Chen,Tao Wang,Haiyan Yu,Zhixiang Zhang 한국유전학회 2012 Genes & Genomics Vol.34 No.5

MicroRNAs are small, non-coding RNAs that regulate gene expression post-transcriptionally and have crucial roles in plant biological and metabolic processes. To date, there has been no report concerning miRNAs in Xanthoceras sorbifolia, one of the most important woody energy plants for biofuel production. To study the roles of miRNAs during floral organ differentiation and development in X. sorbifolia, two small RNA libraries were constructed from the flower buds of simple flowers (wild-type) and double flowers (mutant). Each library was sequenced individually and generated over 33 million raw reads in total. Forty-three conserved miRNAs belonging to 30families and 10 novel miRNAs were identified. The expressions of a selected group of conserved and novel miRNAs were validated by qRT-PCR. Target gene prediction identified 230 target genes for most of the conserved and novel miRNAs. Fifteen miRNAs were demonstrated to be differentially ex-pressed between wild-type and mutant. This is the first study to identify conserved and novel miRNAs and profile their expression changes during floral organ differentiation and development in X. sorbifolia.

Fast and robust misalignment correction of Fourier ptychographic microscopy for full field of view reconstruction

Zhou, Ao,Wang, Wei,Chen, Ni,Lam, Edmund Y.,Lee, Byoungho,Situ, Guohaiz The Optical Society 2018 Optics express Vol.26 No.18

Tensile Properties of Aluminum Alloy Bolt-Sphere Joints under Elevated Temperatures

Hongbo Liu,Ao Gu,Zhi-Hua Chen 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2

Aluminum alloy bolt-sphere joint is one of the major nodes in aluminum alloy space grid structures. It has been applied in many big projects. In this study, computation methods of mechanical properties and bearing capacity of aluminum alloy bolt-sphere joints in fire are proposed by combining experimental research and theoretical analysis. Results demonstrate that: 1) The tensile strength of aluminum alloy bolt-sphere joints in fire is related to the tensile strength of stainless-steel bolt and the tensile strength seal plate joint. 2) The stainless-steel bolt develops two failure modes, namely, breakage failure of cord and pulling-out failure of screw in fire. 3) The seal plate joint in fire has three tensile failure modes, namely, tensile-shearing combined failure of seal plate at the annular compression position, shear failure of seal plate, and tensile failure of the aluminum alloy tube at the seal plate border. 4) The tensile strength of the stainless-steel bolt begins to decrease significantly when the temperature reaches 300°C. 5) The tensile strength of the seal plate joint begins to decrease sharply when the temperature reaches 200°C. 6) The formula to calculate bearing capacity of aluminum alloy bolt-sphere joints in fire is proposed based on experimental results.

Development and manufacturing of a surgical simulator based on virtual reality

Di Cao,Ao Zhang,Xiaojun Chen 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

An XML Approach to Semantically Extract Data from HTML Tables

Liu, J.,Ao, Z.,Park, H.-H.,Chen, Y. SPRINGER-VERLAG 2005 Lecture Notes in Computer Science Vol.3588 No.-

D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation

Bo Qiao,Bin He,Ao Zhou,Yuling Li,Xiaojun Chen,Qiming Yang,Beike Chen,Bo Qiao,Dianming Jiang 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.2

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (DRADA16- RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD). METHODS: Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo. RESULTS: Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/ D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/ D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity. CONCLUSION: This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.

Adaptive Fault Tolerant Stabilization for Uncertain MIMO Systems with Actuation Failures

Wanqin Xiang,Wei Ao,Yiyuan Chen 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.5

This paper considers the state stabilization problem for a class of non-affine uncertain MIMO systems with actuation failures. An adaptive robust fault-tolerant control is developed to compensate the affect of uncertainties, actuator failures. And theoretical analysis based on a Lyapunov-like approach demonstrates that, under some proper conditions, the close-loop system would be stabilized and ultimately uniformly bounded state error could be guaranteed by the controller. Further, the proposed controller is no need for on-line fault detection and diagnosis unit, and inexpensive to compute. At last, numerical simulations are provided to validate and illustrate the benefits and effectiveness of the proposed control scheme.

Extending absorption of near-infrared wavelength range for high efficiency CIGS solar cell via adjusting energy band

Lingling Yan,Yiming Bai,Bo Yang,Nuofu Chen,Zhan'ao Tan,Tasawar Hayat,Ahmed Alsaedi 한국물리학회 2018 Current Applied Physics Vol.18 No.4

The efficient photon harvesting in near infrared wavelength range is still a challenging problem for high performance Cu(In1-x, Gax)Se2 (CIGS) solar cell. Herein, adjusting the energy band distribution of CIGS solar cell could provide significant academic guidance for devices with superior output electric power. To understand the role of each functional layer, the optimal 3000 nm CIGS absorber layer with 1.3 eV bandgap and 30 nm CdS buffer layer were firstly obtained via simulating the uniform band-gap structures. By introducing CIGS absorber layer with a double grading Ga/(GaþIn) profile, the power conversion efficiency of the double gradient band gap cell is superior to that of uniform band-gap cell through extending absorption of near-infrared wavelength range. Upon optimization, the best power conversion efficiency of CIGS with a double gradient band gap solar cell is improved significantly to 24.90%, among the best values reported in literatures, which is an 8.17% relative increase compared with that of the uniform band-gap cell. Our findings provide a theoretical guide toward the design of high performance solar cells and enrich the understandings of the energy band engineering for developing of novel semiconductor devices.