Rocaglamide-A Potentiates Osteoblast Differentiation by Inhibiting NF-κB Signaling

Aiguo Li,Libin Yang,Xiaolin Geng,Xingmei Peng,Tan Lu,Yanjun Deng,Yuzheng Dong 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.11

Rheumatoid arthritis is a chronic inflammatory disease that leads to bone and cartilage erosion. The inhibition of osteoblast differentiation by the inflammatory factor TNF-α is critical for the pathogenesis of rheumatoid arthritis. To modulate TNF-α mediated inhibition of osteoblast differentiation is required to improve therapeutic efficacy of rheumatoid arthritis. Here, we explored the potential role of rocaglamide-A, a component of Aglaia plant, in osteoblast differentiation. Rocaglamide-A prevented TNF-α mediated inhibition of osteoblast differentiation,and promoted osteoblast differentiation directly,in both C2C12 and primary mesenchymal stromal cells. Mechanistically, Rocaglamide- A inhibited the phosphorylation of NF-κB component p65 protein and the accumulation of p65 in nucleus, which resulted in the diminished NF-κB responsible transcriptional activity. Oppositely, overexpression of p65 reversed rocaglamide-A’s protective effects on osteoblast differentiation. Collectively, rocaglamide-A protected and stimulated osteoblast differentiation via blocking NF-κB pathway. It suggests that rocaglamide-A may be a good candidate to develop as therapeutic drug for rheumatoid arthritis associated bone loss diseases.

Rocaglamide-A Potentiates Osteoblast Differentiation by Inhibiting NF-κB Signaling

Li, Aiguo,Yang, Libin,Geng, Xiaolin,Peng, Xingmei,Lu, Tan,Deng, Yanjun,Dong, Yuzheng Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.11

Rheumatoid arthritis is a chronic inflammatory disease that leads to bone and cartilage erosion. The inhibition of osteoblast differentiation by the inflammatory factor TNF-${\alpha}$ is critical for the pathogenesis of rheumatoid arthritis. To modulate TNF-${\alpha}$ mediated inhibition of osteoblast differentiation is required to improve therapeutic efficacy of rheumatoid arthritis. Here, we explored the potential role of rocaglamide-A, a component of Aglaia plant, in osteoblast differentiation. Rocaglamide-A prevented TNF-${\alpha}$ mediated inhibition of osteoblast differentiation, and promoted osteoblast differentiation directly, in both C2C12 and primary mesenchymal stromal cells. Mechanistically, Rocaglamide-A inhibited the phosphorylation of NF-${\kappa}B$ component p65 protein and the accumulation of p65 in nucleus, which resulted in the diminished NF-${\kappa}B$ responsible transcriptional activity. Oppositely, overexpression of p65 reversed rocaglamide-A's protective effects on osteoblast differentiation. Collectively, rocaglamide-A protected and stimulated osteoblast differentiation via blocking NF-${\kappa}B$ pathway. It suggests that rocaglamide-A may be a good candidate to develop as therapeutic drug for rheumatoid arthritis associated bone loss diseases.

Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification

Jie Li,Peiyuan Wang,Aiguo Zhang,Muhamd Alsarakibi,Guoqing Li 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.2

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from 10<SUP>-1</SUP> to 10<SUP>-5</SUP> ng/μl for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of 63˚C by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha (EF1α) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.

β-Catenin promotes long-term survival and angiogenesis of peripheral blood mesenchymal stem cells via the Oct4 signaling pathway

Wang Pengzhen,Deng Zhanyu,Li Aiguo,Li Rongsen,Huang Weiguang,Huang Weiguang,Chen Songsheng,Li Biao,Li Biao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Stem cell therapy has been extensively studied to improve heart function following myocardial infarction; however, its therapeutic potency is limited by low rates of engraftment, survival, and differentiation. Here, we aimed to determine the roles of the β-catenin/Oct4 signaling axis in the regulation of long-term survival and angiogenesis of peripheral blood mesenchymal stem cells (PBMSCs). These cells were obtained from rat abdominal aortic blood. We showed that β-catenin promotes the self-renewal, antiapoptotic effects, and long-term survival of PBMSCs by activating the Oct4 pathway through upregulation of the expression of the antiapoptotic factors Bcl2 and survivin and the proangiogenic cytokine bFGF and suppression of the levels of the proapoptotic factors Bax and cleaved caspase-3. β-Catenin overexpression increased Oct4 expression. β-Catenin knockdown suppressed Oct4 expression in PBMSCs. However, β-catenin levels were not affected by Oct4 overexpression or knockdown. Chromatin immunoprecipitation assays proved that β-catenin directly regulates Oct4 transcription in PBMSCs. In vivo, PBMSCs overexpressing β-catenin showed high survival in infarcted hearts and resulted in better myocardial repair. Further functional analysis identified Oct4 as the direct upstream regulator of Ang1, bFGF, HGF, VEGF, Bcl2, and survivin, which cooperatively drive antiapoptosis and angiogenesis of engrafted PBMSCs. These findings revealed the regulation of β-catenin in PBMSCs by the Oct4-mediated antiapoptotic/proangiogenic signaling axis and provide a breakthrough point for improving the long-term survival and therapeutic effects of PBMSCs.

Evaluation of gaseous concentrations, bacterial diversity and microbial quantity in different layers of deep litter system

Li, Jing,Wang, Jingyu,Wang, Fujin,Wang, Aiguo,Yan, Peishi Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: An experiment was conducted to investigate the environment of the deep litter system and provided theoretical basis for production. Methods: The bedding samples were obtained from a pig breeding farm and series measurements associated with gases concentrations and the bacterial diversity as well as the quantity of Escherichia coli, Lactobacilli, Methanogens were performed in this paper. Results: The concentrations of $CO_2$, $CH_4$, and $NH_3$ in the deep litter system increased with the increasing of depth while the $N_2O$ concentrations increased fiercely from the 0 cm to the -10 cm depth but then decreased beneath the -10 cm depth. Meanwhile, the Shannon index, the dominance index as well as the evenness index at the -20 cm layer was significantly different from the other layers (p<0.05). On the other hand, the quantity of Escherichia coli reached the highest value at the surface beddings and there was a significant drop at the -20 cm layer with the increasing depth. The Lactobacilli numbers increased with the depth from 0 cm to -15 cm and then decreased significantly under the -20 cm depth. The expression of Methanogens reached its largest value at the depth of -35 cm. Conclusion: The upper layers (0 cm to -5 cm) of this system were aerobic, the middle layers (-10 cm to -20 cm) were micro-aerobic, while that the bottom layers (below -20 cm depth) were anaerobic. In addition, from a standpoint of increasing the nitrification pathway and inhibiting the denitrification pathway, it should be advised that the deep litter system should be kept aerobic.

Research on Deep-Sea Pipeline Tube Bundle Heating System

Li Ding,Xinghua Ding,Weina Ren,Yingying Mu,Aiguo Lin 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.6

In order to ensure the safety of deep-sea submarine pipelines, this paper focuses on the analysis of the axial temperature distribution model of the submarine pipeline and the distribution parameter circuit model of the tube bundle heating system. Combined with these two models, theoretical analysis shows that the heating eff ect of the tube bundle heating system depends on the distributed circuit parameters and power frequency of the system. In order to improve the heat tracing effi ciency, the power frequency needs to be adjusted according to the change of the load temperature, so the power supply frequency of the tube bundle heating system based on the Hammerstein model is optimized. Using a neuro-fuzzy algorithm, not only the theoretical values of heating power and power frequency are obtained, but also the drawbacks of determining the above parameters based on engineering experiments are avoided. Moreover, the pipeline heating is effi cient and stable, the dynamic response is fast, and the working condition is also well adapted.

Primary Current Generation for a Contactless Power Transfer System Using Free Oscillation and Energy Injection Control

Li, Hao Leo,Hu, Aiguo Patrick,Covic, Grant Anthony The Korean Institute of Power Electronics 2011 JOURNAL OF POWER ELECTRONICS Vol.11 No.3

This paper utilizes free oscillation and energy injection principles to generate and control the high frequency current in the primary track of a contactless power transfer system. Here the primary power inverter maintains natural resonance while ensuring near constant current magnitude in the primary track as required for multiple independent loads. Such energy injection controllers exhibit low switching frequency and achieve ZCS (Zero Current Switching) by detecting the high frequency current, thus the switching stress, power losses and EMI of the inverter are low. An example full bridge topology is investigated for a contactless power transfer system with multiple pickups. Theoretical analysis, simulation and experimental results show that the proposed system has a fast and smooth start-up transient response. The output track current is fully controllable with a sufficiently good waveform for contactless power transfer applications.

Challenges in neuro-machine interaction based active robotic rehabilitation of stroke patients

Song, Aiguo,Yang, Renhuan,Xu, Baoguo,Pan, Lizheng,Li, Huijun Techno-Press 2014 Advances in robotics research Vol.1 No.2

Study results in the last decades show that amount and quality of physical exercises, then the active participation, and now the cognitive involvement of patient in rehabilitation training are known of crux to enhance recovery outcome of motor dysfunction patients after stroke. Rehabilitation robots mainly have been developing along this direction to satisfy requirements of recovery therapy, or focusing on one or more of the above three points. Therefore, neuro-machine interaction based active rehabilitation robot has been proposed for assisting paralyzed limb performing designed tasks, which utilizes motor related EEG, UCSDI (Ultrasound Current Source Density Imaging), EMG for rehabilitation robot control and feeds back the multi-sensory interaction information such as visual, auditory, force, haptic sensation to the patient simultaneously. This neuro-controlled and perceptual rehabilitation robot will bring great benefits to post-stroke patients. In order to develop such kind of robot, some key technologies such as noninvasive precise detection of neural signal and realistic sensation feedback need to be solved. There are still some grand challenges in solving the fundamental questions to develop and optimize such kind of neuro-machine interaction based active rehabilitation robot.

Improved AQPSO Algorithm for Solving the Model of the Skin Effect Electric Heating System

Ding Li,Ding Xinghua,Ren Weina,Lin Aiguo 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

In view of the importance of the skin-eff ect electric heat tracing system of the submarine oil pipeline in heating and condensation reduction, it is necessary to start from the skin electric heating mechanism and construct its complete mathematical model to realize the effi cient operation of the system. Aiming at the problem of unknown parameters in the model of the skin-eff ect electric heat tracing system, an improved adaptive quantum behaviour particle swarm optimization algorithm (AQPSO) based on the quantum behaviour particle swarm optimization algorithm is proposed, in order to eff ectively identify the model parameters. In the algorithm design, in view of the high dependence of the basic QPSO algorithm on the shrinkage and expansion coeffi cients, the particle aggregation factor is introduced, and the shrinkage and expansion coeffi cients are redesigned. In view of the diff erent proportions of the fi tness value of the individual optimal position, the weight coeffi cient is introduced to construct the best position of the average weight to realize the improvement of the basic QPSO algorithm. The simulation results show that the AQPSO algorithm has better performance than QPSO in terms of convergence accuracy and robustness. This algorithm not only overcomes the inherent premature defects of particle swarms, but also improves the accuracy of the algorithm and obtains accurate model parameters. It can be seen that the improved adaptive quantum behaviour particle swarm algorithm has certain feasibility and eff ectiveness in the parameter identifi cation of Hammerstein model.

Safe Arm Using Compliant Hybrid Joints for Mobile Manipulators in Human-Symbiotic Environments

Zhijun Li,Aiguo Ming,Ning Xi,Jiangong Gu,Makoto Shimojo 한국과학기술원 인간친화 복지 로봇 시스템 연구센터 2005 International Journal of Assistive Robotics and Me Vol.6 No.1

  In this paper, a safe arm with compliant hybrid joints for human-friendly service robots is presented, which realizes human safety, absorbing impact force, and task fulfillment. The robot is composed of links covered with soft materials and hybrid joints, which can be put into active or passive mode as needed. In an unexpected or expected collision with human, the arising impulse force is attenuated effectively by the physical model, which is developed with hybrid joints and soft covering. Owing to the displacement of the links when the joint is passive, a recovery control algorithm has been developed for the end-effector to maintain its desired task position after the collision. The force attenuation property has been verified through collision experiments in that the capability of the proposed passive arm in overcoming the limitations of active compliance control has been demonstrated. Simulation results also showed that the proposed control method is useful for robot"s task fulfillment as well.