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김치로부터 분리된 Leuconostoc sp. strain YSK 균주에 의한 덱스트란 생산 조건의 최적화
황승균(Seung Kyun Hwang),홍준택(Jun Taek Hong),정경환(Kyung Hwan Jung),장병철(Byung Chul Chang),황경숙(Kyung Suk Hwang),신정희(Jung Hee Shin),임성팔(Sung Paal Yim),유선균(Sun Kyun Yoo) 한국생명과학회 2008 생명과학회지 Vol.18 No.10
본 연구는 발효 김치 액으로부터 덱스트란 생산 균주를 분리하고 생산 최적 생산 조건을 설정하기 위하여 반응표면분석법을 이용하였다. 발효 조건의 독립변수들은 배양 온도, pH, 효모 추출물의 농도, 온도, 기질의 농도로 정하고 Box-Benken 디자인를 이용하여 실험을 설계하였다. 최종 분리된 균주는 Leuconost℃ sp. strain SKY로 잠정적으로 명하였다. 연구 결과 덱스트란 생산은 3.90 - 22.40 g/l이고, 균체량 생산은 0.69-2.85 g/l, 수율은 0.10-0.64, 생산 속도 0.16-0.85 g/l-hr의 범위에서 분석이 되었다. 표면반응분석 결과 덱스트란 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 균체량 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 생산 수율에 가장 영향을 미치는 것은 배양 pH 이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 덱스트란 생산 속도에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 결론적으로 최적 생산 조건은 온도는 27-28℃이고, pH는 7.0이며, 효묘 추출물은 6-7%의 범위에서 결정이 되었다. 이러한 조건에서 생산된 덱스트란양은 22g/l이고, 생산 수율은 약 60%정도이며, 생산 속도는 0.8g/l/hr이었다. A bacterium producing non- or partially digestible dextran was isolated from kimchi broth by enrichment culture technique. The bacterium was identified tentatively as Leuconostoc sp. strain SKY. We established the response surface methodology (Box-Behnken design) to optimize the principle parameters such as culture pH, temperature, and yeast extract concentration for maximizing production of dextran. The ranges of parameters were determined based on prior screening works done at our laboratory and accordingly chosen as 5.5, 6.5, and 7.5 for pH, 25, 30, and 35℃ for temperature, and 1, 5, and 9 g/l yeast extract. Initial concentration of sucrose was 100 g/l. The mineral medium consisted of 3.0 g KH₂PO₄, 0.01 g FeSO₄ㆍH₂O, 0.01 g MnSO₄ㆍ4H₂O, 0.2 g MgSO₄ㆍ7H₂O, 0.01 g NaCl, and 0.05 g CaCO₃ per 1 liter deionized water. The optimum values of pH and temperature, and yeast extract concentration were obtained at pH (around 7.0), temperature (27 to 28℃), and yeast extract (6 to 7 g/l). The best dextran yield was 60% (dextran/g sucrose). The best dextran productivity was 0.8 g/h-l.
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박철기,황승균,곽호신,유헌,정영섭,백선하,김동규,정희원,김성연,홍승관,Park, Chul-Kee,Hwang, Sung Kyun,Gwak, Ho-Shin,Yoo, Heon,Chung, Young Seob,Paek, Sun Ha,Kim, Dong Gyu,Jung, Hee-Won,Kim, Seong Yeon,Hong, Seung Kuan 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.10
Objectives : The authors analyzed the surgical series of Cushing's disease to evaluate the proper treatment policy and to verify the possible prognostic factors. Material and Methods : Of 50 patients diagnosed as Cushing's disease and operated at Department of Neurosurgery of our institute between 1988 and 1999, 48 patients with available medical records were analyzed retrospectively. Mean follow-up period was 48 months(3 to 109 months). Preoperative diagnosis was made after evaluating the patients with multiple-stage endocrinological studies and 31 selective patients were evaluated with inferior petrosal sinus sampling(IPSS). Magnetic resonance imaging(MRI) and/or high resolution computerized tomography(CT) was done in all patients. A total of 51 transsphenoidal adenomectomy(TSA) were performed including 3 revision for initial surgical failure cases. Remission was decided on the basis of both endocrinological criteria and clinical status. Radiation and/or ketoconazole therapy were applied to failed cases. For the verification of prognostic factors, the authors evaluated the statistical significance of multiple variables over remission rate by chi-square test. Result : Sensitivity of IPSS for central localization was 93.5% which was better than that of MRI(87.5%). But for lateralization, it was 72.4% for IPSS versus MRI 90.5%. Success rate of TSA was 82%(42/51) and recurrence rate was 9%(4/48). When including adjuvant treatments for surgically failed cases, overall success rate was 89.6% and all of 3 reoperated cases(TSA) due to recurrence were successful. Significant complication occurred in 7.8%(4/51) after TSA including hypopituitarism, diabetes insipidus, and visual loss. Non-existence of tumor in MRI and prolonged symptom duration(>3 years) were significant prognostic factors. Conclusion : TSA can be considered as initial treatment for Cushing's disease. In surgically failed cases, multiple treatment modality may improve the overall outcome and repeated TSA for recurrent cases seem to provide similar success.
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성상세포종과 수막종으로 오인된 유두상 상의세포종 -증례보고-
이승준 ( Seung Joon Lee ),김정은 ( Jeong Eun Kim ),황승균 ( Sung Kyun Hwang ),정희원 ( Hee Won Jung ),최윤라 ( Yoon La Choi ) 대한뇌종양학회 2003 대한뇌종양학회지 Vol.2 No.2
The authors report a case of papillary ependymoma, which is known to be rare and difficult to diagnose. A 35-year-old female, who had been previously diagnosed as having anaplastic astrocytoma at the other hospital, was admitted with headache, vomiting and decreased visual acuity. She had a multicystic tumor in the left parietal lobe on magnetic resonance image(MRI), and initial pathologic diagnosis after surgical resection was papillary meningioma. However, the electronmicroscopy( EM) examination showed findings of papillary ependymoma. In spite of adjuvant radiotherapy, postoperative 1-year follow-up MRI showed recurrence of tumor. The tissue obtained from the recurred tumor showed anaplastic features of dedifferentiated tumor cells with a proliferation index(Ki-67 index) of 10%. Supratentorial papillary ependymoma is difficult to be diagnosed only by typical light microscopy or immunohistochemistry. Therefore, ultrastructural study is necessary for diagnosis of papillary ependymoma.
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댄디-워커 기형이 동반된 다수의 선천성 멜라닌 세포 모반
류소민 ( So Min Lyu ),이상우 ( Sang Woo Lee ),정승현 ( Seung Hyun Cheong ),명기범 ( Ki Bum Myung ),최유원 ( You Won Choi ),황승균 ( Sung Kyun Hwang ),최혜영 ( Hae Young Choi ) 대한피부과학회 2012 대한피부과학회지 Vol.50 No.1
Congenital melanocytic nevi (CMN) are benign pigmented lesions found in about 1% of all newborns or shortly after birth. Giant melanocytic nevi, with multifocal involvement, show significantly greater risk of developing malignant melanomas and neurocutaneous melanosis (NCM), particularly in a posterior axial location. NCM is a rare congenital disease characterized by multiple (≥3) small nevi, or at least one large congenital melanocytic nevus in combination with cerebral and/or leptomeningeal melanin deposits or melanoma. Dandy-Walker malformation (DWM) consists of a cystic dilatation of the fourth ventricle, hypoplasia or aplasia of the cerebellar vermis, and enlarged posterior fossa with or without hydrocephalus. The association of DWM and NCM has rarely been reported in the literature. A 3 month-old girl presented with increased head circumference and multiple various sized black plaques on her whole body. She underwent a ventriculoperitoneal shunt operation when she was 2 months-old. A skin biopsy was taken from the largest and darkest plaque of the trunk and showed hyperpigmentation of the basal layer of the epidermis. The dermis contained nevus cells in nests and sheets throughout the dermis, but no cellular atypia was noted. Magnetic resonance image (MRI) of the brain revealed severe hydrocephalus with hypoplasia of cerebellar vermis and agenesis of cerebellar tonsil which are consistent with Dandy-Walker malformation. We recommended a spinal MRI for check up the presence of leptomeningeal melanosis, but could not evaluate the result because of her being adapted. Herein, we present a rare case of multiple congenital melanocytic nevi in association with DWM in a neonate. (Korean J Dermatol 2012;50(1):60∼66)
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