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Tc-99m 표지 항과립구항체 면역신티그라피를 이용한 골수염의 진단
강원준,정준기,여정석,홍미경,정재민,이동수,이상훈,최인호,이명철 ( Won Jun Kang,June Key Chung,Jeong Seok Yeo,Mee Kyoung Hong,Jae Min Jeong,Dong Soo Lee,Sang Hoon Lee,In Ho Choi,Myung Chul Lee ) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4
Purpose: The purpose of this study was to evaluate the diagnostic accuracy of Tc-99m labeled antigranulocyte antibody immunoscintigrapy in the diagnosis of osteomyelitis and compare with the results of triphasic bone scan. Materials and Methods: The study popula- tion was 39 patients (22 male, 17 female) who had uncertain diagnoses of osteomyelitis. Fifteen patients had history of orthopedic surgery, and 5 had previous fracture. One milligram of monoclonal antibody against NCA-95 was labeled with 370 MBq of Tc-99m, injected intravenously, and 4 hour images were obtained. Triphasic bone scan images were obtained in 30 p;tients. The final diagnosis was confirmed by bacteriologic culture, biopsy or long term clinical follow up. Results: Twenty one patients were confirmed to have osteomyelitis (1 acute, 20 chronic). Eighteen patients were without osteomyelitis. Antigranulocyte antibody immunoscintigraphy had a sensitivity of 71% (15/21), and a specificity of 89% (16/18), while the sensitivity and specificity of triphasic bone scan was 93% (13/14) and 38% (6/16), respectively. Antigranulocyte antibody scan showed higher specificity of 100% (11/11) in comparison with 33% (3/9) of triphasic bone scan in patients with history of orthopedic surgery or fracture. Conclusion: Antigranulocyte antibody immunoscintigraphy is more specific than that of triphasic bone scan and may be helpful in patients with history of surgery or fracture. However, sensitivity is lower than triphasic bone scan in the detection of chronic osteomyelitis. (Korean J Nucl Med 1998;32:344-53)
국산 항 CEA 항체의 I - 131 , Tc - 99m 표지법 확립 및 면역학적 특성 분석
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),홍미경(Mee Kyoung Hong),최석례(Seok Rye Choi),서일택(Il Taek Seo),정홍근(Hong Keun Chung),정준호(Jun Ho Chung) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.2
N/A Caneer cells have several tumor-associated antigens on the cell surfaces, and antibodies against these antigens have been developed by many investigators. Radiolabeled antibodies have been used as new methods to diagnose and treat malignant tumors. Especially anti- carcinoembryonic antigen (CEA) is the most popular antibody for these purposes. In this investigation, we tried to label 131I and Tc-99m to anti-CEA monoclonal antibodies which were developed in the Seoul National University College of Medicine. We found CEA-79 and CEA-92 antibodies had the better immunological characteristics among 8 anti-CEA monoclonal antibodies. And radioiodination of CEA-79 could be performed by chloramine-T method, while radioiodination of CEA-92 by iodogen method. To label these antibodies with Tc-99m, we used pretargeting transchelation as direct labeling method. At first, Tc-99m was bound to glucaric acid, and monoclonal antibody was reduced by β-mercaptoethanol. When these were incubated together, Tc -99m bound to glucarate was switched to monoclonal antibody because of higher affinity. We established conditions of several steps in this method. Anti-CEA monoclonal antibodies labeled with 131I and Tc-99m are expected to be used valuably in the detection and treatment of malignant tumors.
사람 암세포와 단핵세포에서 고포도당 농도에 의한 FDG 섭취 저하의 서로 다른 기전
이동수(Dong Soo Lee),정준기(June Key Chung),이명철(Myung Chul Lee),김채균(Chae Kyun Kim),이용진(Yong Jin Lee),홍미경(Mee Kyoung Hong),정재민(Jae Min Jeong) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2
N/A To clarify the difference in glucose uptake between human cancer cells and monocytes, we studied [^18F] fluorodeoxyglucose (FDG) uptake in three human colon cancer cell lines (SNU-C2A, SNU-C4, SNU-C5), one human lung cancer cell line (NCI-H522), and human peripheral blood monocytes. The FDG uptake of both cancer cells and monocytes was increased in glucose-free medium, but decreased in the medium containing 16.7 mM glucose (hyperglycemic). The levelof Glut1 mRNA decreased in human colon cancer cells and NCI-H522 under hyperglycemic condition. Glut1 protein expression was also decreased in the four human cancer cell lines under hyperglycemic condition, whereas it was consistently undetectable in monocytes. SNU-C2A, SNU-C4 and NCI-H522 showed a similar level of hexokinase activity (7.5-10.8mU/mg), while SNU-C5 and monocytes lower range of hexokinase activity (4.3-6.5 mU/mg). These data suggest that glucose uptake is regulated by different mechanisms in human cancar cells and monocytes. (Korean J Nucl Med 2002;36;110-120)
Re-188과 Tc-99m 표지 단일클론항체 CEA79.4의 생체외 특성과 생체내 분포
이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),장영수(Young Soo Chang),홍미경(Mee Kyoung Hong),여정석(Jeong Seok Yeo),이용진(Yong Jin Lee),김경민(Kyung Min Kim),이승진(Seung Jin Lee) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.6
N/A Purpose: Radiolabeled CEA79.4 antibody has a possibility to be used in radioimmunoscintigraphy or radioimmunotheraphy of cancer. We investigated the in vitro properties and biodistribution of CEA79.4 antibody labeled with Re-188 or Tc-99m. Materials and Methods: CEA79.4 was reduced by 2-mercaptoethanol to produce-SH reside, and was labeled with Re-188 or Tc-99m. For direct labeling of Tc-99m, methylene-diphosphonate was used as transchelating agent. CEA79.4 in 50 mM Acetate Buffered Saline (ABS, pH 5.3) was labeled with Re-188, using stannous tartrate as reducing agent. In order to measure immunoreactivity and the affinity constant of radiolabeled antibody, cell binding assay and Scatchard analysis using human colon cancer cells SNU-C4, were performed. Biodistribution study of labeled CEA79.4 was carried out at 1, 14 and 24 hr in ICR mice. Results:. Labeling efficiencies of Tc-99m and Re-188 labeled antibodies were 92.4±5.9% and 84.7±4.6%, respectively. In vitro stability of Tc-99m-CEA79.4 in human serum was higher than Re-188-CEA79.4. Immunoreactivity and affinity constant of Tc-99m-CEA79.4 were 59.2% and 6.59×109 M-1, respectively, while those of Re-188-CEA79.4 were 41.6% and 4.2×109 M-1, respectively. After 24 hr of administrations of Re-188 and Tc-99m labeled antibody, the remaining antibody, the remaining antibodies in blood were 6.32 and 9.35% ID/g respectively. The biodistribution of each labeled antibody in other organs was similar because they did not accumulate in non-targeted organs. Conclusion: In vitro properties and biodistribution of Re-188-CEA79.4 were similar to those of Tc-99m-CEA79.4. It appears that Re-188-CEA79.4 can be used as a suitable agent for radioimmunotherapy.
뇌혈류 평가용 99mTc - HMPAO 합성 및 분포에 관한 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),조보연(Bo Youn Cho),이범우(Bum Woo Lee),정재민(Jae Min Jeong),염미경(Mi Kyung Yeom),홍미경(Mee Kyoung Hong),최석례(Seok Rye Choi) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.2
N/A HMPAO was synthesized by two step reaction. d, 1-HMPAO was separated from the racemic product by fractional recrystalization in ethylacetate, and the chemical structure and purity was confirmed by proton NMR spectroscopy. The synthesized d, 1-HMPAO was labeled with Tc- 99m and studied the biodistribution in mice. From the results we could find that liver uptake of synthesized Tc-99m d, 1-HMPAO was higher than that of Amersham kit, but no conspicuous difference was found in brain and other tissues (blood, lung, stomach, intestine, muscle, spleen and kidney).
항 NCA - 95 단일클론항체의 99mTc 표지 키트 제조 및 특성 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),김채균(Chae Kyun Kim),홍미경(Mee Kyoung Hong),최석례(Seok Rye Choi),이용진(Yong Jin Lee) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A The previous monoclonal antibody labeling method for bone marrow immunoscintigrapy was complicated and laborious for clinical application. Also it showed a relatively low labeling efficiency. To improve this procedure, we compared several direct labeling methods of Tc-99m. 1) The labeling efficiency in the method using gluconate as a transchelator was low (40-70%), but immunoscintigraphy using this radiotracer produced a clear image. 2) To improve labeling efficiency, β-mercaptoethanol was removed after reduction. The labeling efficiency was improved up to 70-80%, but the radioactivity of the blood pool was high. 3) The higest labeling efficiency (〉90%) and best quality images could be obtained by using MDP as a transchelating agent. It did not require additional procedures for separation of labeled antibodies. The immunoreactivity of this antibody was 60%. Residual MDP which can be taken up by the bone could be removed by PD-10 column. The reduced antibodies were stable with a high labeling efficiency (〉90%) for up to 47 days by deep freezing. We concluded that the improved procedure for Tc-99m labeling of anti-NCA-95 monoclonal antibody using MDP as a transchelating agent will be a simple and useful method for clinical application.