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원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(II) - 융합균사체의 항암성분이 생쥐의 면역세포에 미치는 영향 -
문철,김채균,윤종명,심미자,김하원,최응칠,김병각,Moon, Chul,Kim, Chae-Kyun,Yoon, Jong-Myung,Shim, Mi-Ja,Kim, Ha-Won,Choi, Eung-Chil,Kim, Byong-Kak 한국생약학회 1996 생약학회지 Vol.27 No.3
The antitumor components of the protoplast fusants of Lentinula edodes and Ganoderma lucidum were examined for immunological activity to elucidate the mechanism of their antitumor activity. They did not show any direct cytotoxicity against tumor cells. But being examined for immunopotentiation activity, they increased the number of colonies in the bone marrow stem cells to 3.0 times. They also increased the activities of the acid phosphatase in activated macrophages to 2.1 times and the secretion of nitric oxide in RAW 264.7 to 2.2 times, respectively. They activated the components of the alternative complement pathway. In humoral immunity. they increased the activities of the alkaline phosphatase in differentiated B cells to 1.6 times and the number of plaque forming cells to 1.8 times, respectively. In cellular immunity, they restored the depressed response of delayed type hypersensitivity in tumor bearing mice to normal level.
원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(I) - 융합균사체의 항암성분 -
문철,윤종명,김채균,김하원,최응칠,김병각,Moon, Chul,Yoon, Jong-Myung,Kim, Chae-Kyun,Kim, Ha-Won,Choi, Eung-Chil,Kim, Byong-Kak 한국생약학회 1996 생약학회지 Vol.27 No.3
To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.
사람 암세포와 단핵세포에서 고포도당 농도에 의한 FDG 섭취 저하의 서로 다른 기전
이동수(Dong Soo Lee),정준기(June Key Chung),이명철(Myung Chul Lee),김채균(Chae Kyun Kim),이용진(Yong Jin Lee),홍미경(Mee Kyoung Hong),정재민(Jae Min Jeong) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2
N/A To clarify the difference in glucose uptake between human cancer cells and monocytes, we studied [^18F] fluorodeoxyglucose (FDG) uptake in three human colon cancer cell lines (SNU-C2A, SNU-C4, SNU-C5), one human lung cancer cell line (NCI-H522), and human peripheral blood monocytes. The FDG uptake of both cancer cells and monocytes was increased in glucose-free medium, but decreased in the medium containing 16.7 mM glucose (hyperglycemic). The levelof Glut1 mRNA decreased in human colon cancer cells and NCI-H522 under hyperglycemic condition. Glut1 protein expression was also decreased in the four human cancer cell lines under hyperglycemic condition, whereas it was consistently undetectable in monocytes. SNU-C2A, SNU-C4 and NCI-H522 showed a similar level of hexokinase activity (7.5-10.8mU/mg), while SNU-C5 and monocytes lower range of hexokinase activity (4.3-6.5 mU/mg). These data suggest that glucose uptake is regulated by different mechanisms in human cancar cells and monocytes. (Korean J Nucl Med 2002;36;110-120)
사람 대장암 세포주의 [ 18F ] fluorodeoxyglucose 섭취의 특징
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),정재민(Jae Min Jeong),김채균(Chae Kyun Kim) 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.3
N/A Cancer tissues are characterized by increased glucose uptake. 18F-fluorodeoxyglucose(FDG), a glucose analogue is used for the diagnosis of cancer in PET studies. This study was aimed to compare the glucose uptake and glucose transporter l(GLUT1) expression in various human colon cancer cells. We measured FDG uptake by cell retention study and expression of GLUTI using Western blotting. Human colon cancer cells, SNU-C2A, SNU-C4 and SNU-C5, were used. The cells were incubated with 1μCi/ml of FDG in HEPES-buffered saline for one hour. The FDG uptake of SNU-C2A,SNU-C4 and SNU-C5 were 16.8±1.36, 12.3±5.55 and 61.0±2.17cpm/μg of protein, respectively. Dose-response and time-course studies represent that FDG uptake of cancer cells were dose dependent and time dependent. The rate of FDG uptake of SNU-C2A, SNU-C4 and SNU-C5 were 0.29±0.03, 0.21±0.09 and 1.07±0.07cpm/min/μg of protein, respectively. Western blot analysis showed that the GLUT1 expression of SNU-C5 was significantly higher than those of SNU-C2A and SNU-C4. These results represent that FDG uptake into human colon cancer cells are different from each other. In addition, FDG uptake and expression of CLUT1 are closely related in human colon cancer cells.
실험적 뇌허혈증 모델에서 허혈 조직의 99mTc - glucarate 섭취
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),김영주(Young Ju Kim),정재민(Jae Min Jeong),김채균(Chae Kyun Kim),최석례(Seok Rye Choi),마응천(Woong Chun Mar) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A To detect ischemic tissue in experimental model of cerebral ischemia made by middle cerebral artery(MCA)-occlusion, we acquired triple image of Tc-99m-glucarate, [18F]fluoro-deoxyglucose (FDG), and 2,3,5-triphenyltetrazolium (TTC) staining. We made cerebral infarction either with reperfusion (after occlusion of 2 hours) or without reperfusion in 10 Sprague-Dawley rats by inserting thread to MCA through internal carotid artery. After 22 hours, we injected 740 MBq of Tc-99m-glucarate and 55.5 MBq of [18F]FDG through tail vein. Each 1 mm slice of rat brains was frozen and exposed to imaging plate for 20 minutes in freezer to get an [18F]FDG image. After 20 hours enough to fade radioactivity of [18F]FDG, the slices were again imaged by BAS1500 for Tc-99m-glucarate uptake·Finally, these braim tissues were stained with TTC. semi-quantitative visual analysis was done by grading 0 to 3 points according to the degree of uptakes(Tc-99m-glucarate) and decreased uptakes([18F]FDG and TTC). Ten rats survived with neurologic symptoms. TTC staining confirmed the development of infarction. The size of the infarction was relatively larger in the group without reperfusion. [18F]FDG images were similar to TTC-stained images. However, we found regions with intermediate uptake which were not stained with TTC. We found regions with intermediate [18F]FDG uptake where TTC staining was normal. Tc-99m-glucarate uptake was found only in TTC non-stained region. In the TTC stained regions, there were no uptake of Tc-99m-glucarate. We could not find clear relation between Tc-99m- glucarate uptake with [18F]FDG uptake. This was partly because percent uptake of Tc-99m- glucarate was so small (less than 1 percent of injected dose) and because there were quite heterogeneity of patterns of [18F]FDG uptake and TTC. With these findings, we could conclude that Tc-99m-glucarate were taken up only in part of ischemic tissues which were proven to be nonviable. The establishment of MCA-occluded rat model with or without reperfusion and triple imaging for Tc-99m, 18F and TTC helped the characterzation of Tc-99m-glucarate uptakes. Further work is needed to clarify the meaning of diversities of [18F]FDG and TTC and their relation with Tc-99m-glucarate.
항 NCA - 95 단일클론항체의 99mTc 표지 키트 제조 및 특성 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),김채균(Chae Kyun Kim),홍미경(Mee Kyoung Hong),최석례(Seok Rye Choi),이용진(Yong Jin Lee) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A The previous monoclonal antibody labeling method for bone marrow immunoscintigrapy was complicated and laborious for clinical application. Also it showed a relatively low labeling efficiency. To improve this procedure, we compared several direct labeling methods of Tc-99m. 1) The labeling efficiency in the method using gluconate as a transchelator was low (40-70%), but immunoscintigraphy using this radiotracer produced a clear image. 2) To improve labeling efficiency, β-mercaptoethanol was removed after reduction. The labeling efficiency was improved up to 70-80%, but the radioactivity of the blood pool was high. 3) The higest labeling efficiency (〉90%) and best quality images could be obtained by using MDP as a transchelating agent. It did not require additional procedures for separation of labeled antibodies. The immunoreactivity of this antibody was 60%. Residual MDP which can be taken up by the bone could be removed by PD-10 column. The reduced antibodies were stable with a high labeling efficiency (〉90%) for up to 47 days by deep freezing. We concluded that the improved procedure for Tc-99m labeling of anti-NCA-95 monoclonal antibody using MDP as a transchelating agent will be a simple and useful method for clinical application.