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함태호,임다은,권순욱,류수노 한국육종학회 2019 한국육종학회지 Vol.51 No.2
‘Superhongmi’, a reddish brown rice cultivar, was derived from a cross between CG2-3-5-1-6-1 (Heugjinju/Suwon 425) producing blackish purple rice and ‘Daeribbyeo 1’ producing large white grains. A promising line, SR28721-7-5-2-1-2-1, was obtained using the pedigree breeding method from 2003 to 2015 and designated as ‘KNOU6R’. This variety headed on September 5, and its culm length is 94.7 cm. The panicle length of ‘Superhongmi’ is 19.8 cm. The number of panicles per hill and grain per panicle is 5.4 ea and 154.9 ea, respectively. The fertility ratio of ‘Superhongmi’ is approximately 91.0% and 1000-grain weight (TGW) is approximately 26.8 g, which is similar to that of ‘Superjami’; however, the number of panicles per hill is half of that of ‘Superjami’. The content of taxifolin and total polyphenol extracted from whole grains of ‘Superhongmi’ is 67.7 and 248 mg/100g seed weight, respectively (Registration No. 7365).
함태호,권순욱,류수노 한국육종학회 2016 한국육종학회지 Vol.48 No.4
‘Pareunsuperjami’, a early maturing and blackish purple pigmented rice cultivar, was developed from a cross between Heugjinju and Suwon 425. During selected by pedigree breeding method until F8 generation, a promising line, C3GM(CG2-2-70-1-1-3-2-B), was selected and advanced by early maturing and contents of cyanidin 3-glucoside. Advance line designated as the name of 'KNOU 3'. This variety headed on July. 30, 115 days to heading after sowing. The culm length and the panicle length of 'Pareunsuperjami' was 63.5cm and 21.3cm, respectively. The number of panicle per hill was 9.4 and the number of grain per panicles was 98.0. The ratio of fertility of ' Pareunsuperjami’ was about 80.5% and 1,000-grain weight was about 18.8 g, heavier than that of ‘Heugjinju’. ' Pareunsuperjami ' has 3~4 times higher Cyanidine-3-glucoside(C3G) contents compared with ‘Heugjinju’.
Genome-Wide Association Study of Blast Resistant in Korean Rice (Oryza sativa L.) Breed Lines
함태호,이자홍,장성규,김무현,장홍지아,김나은,권순욱,이주현 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.2
A total of 857 rice breed lines were used to evaluate rice blast resistance. Frequency of leaf spot index was skewed to theright of the 1-9 scale in bar plot, with a score of 7 showing the highest frequency. The average spot index score of 857 breed lines was5.33. Associations showing higher than the threshold of ‒log10(P) = 5.17 were detected as significant associations. Significantlyassociated single nucleotide polymorphism (SNP) markers located within ± 250 kb on the lead SNP position was designated to one QTLlocus of lead SNP markers. Five association loci were detected. Two associated QTLs detected on Chr. 4 were designated as qRB4.1and qRB422, explaining 17.8% and 14.3% of total phenotypic variations, respectively. Associated QTLs detected on Chr. 1, 11, and 12(one each) designated as qRB1, qRB11 and qRB12 explained 44.6%, 9.09%, and 13.7% of total phenotypic variations, respectively. Wecompared previously reported QTLs. The location of qRB4.2 was overlapped with the previously reported QTL for blast fieldresistance. The location of qRB12 was also overlapped with the field resistance leaf blast. The other one, qRB4.1, was overlapped withbacterial blight resistance.
대립, 천연색소 C3G 고함유 “대립자미” 기능성 신품종 쌀의 이화학적 특성
함태호,류수노,강미영 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
흑진주벼에 비해 천연색소 C3G(Cyanidin-3-glucoside)가 3.8배 이상 높으며 종실의 크기가 1.7배 큰 대립자미(국립종자원 품종등록 : 제4150호, 2012. 10. 17)의 이화학적 특성을 밝혀 기능성 쌀 이용의 기초자료를 확립하고, 기존의 쌀과의 차이점을 밝히고자 수행하였다. 일반성분 분석은 AOAC법, 쌀 배유의 단백질 함량은 Foss Tecator로 측정하였다. 유색미 70% 에탄올 추출물의 기능성 물질 분석은 Singleton(1965)의 방법, Jia 등(1999)의 방법을 수정하여 실험하였다. 일반성분의 경우 수분함량은 일품벼가 가장 높았고, 흑진주, 대립자미, 슈퍼자미 순이었고, 식미와 관계가 있는 조단백질과 조지방함량은 흑진주벼보다 낮아 대립자미의 취반특성이 우수한 것으로 평가되었다. 아밀로스 함량은 밥의 부피와 끈기, 노화지연에 관계가 있는데, 대립자미가 낮아 기존의 유색미보다 취반특성이 좋은 것으로 확인되었다. 대립자미의 1,000립중은 28.1g으로 흑진주벼보다 1.7배, 천연색소 C3G 함량은 3.8배 높은 특징을 가진 품종으로 항산화 생리활성을 가지는 총 폴리페놀 함량과 전자공여능을 측정한 결과 대립자미는 높은 생리기능성을 가진 품종으로 확인되었다. 슈퍼자미의 총 항상화력이 375.34 AEAC로 가장 높은 값을 나타내었으며 대립자미가 355.92, 흑진주가 274.58의 값을 나타내었다. Hydroxy radical 소거능과 SOD 유사활성 역시 다른 항산화 측정 결과와 유사하게 대립자미와 슈퍼자미에서 일반현미와 흑진주보다 유의적으로 높은 활성을 나타낸다. 유기 용매의 극성에 따른 슈퍼자미와 대립자미의 분획추출물의 DPPH 라디칼소거능은 비슷한 경향을 나타냈다.
천연색소 C3G 고함유 만생 대립 ‘슈퍼자미2호’ 벼 품종
함태호(Tae-Ho Ham),권순욱(Soon Wook Kwon),류수노(Su-Noh Ryu) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
‘Superjami 2’, a new blackish purple pigmented rice cultivar was derived from a cross between CG2-3-5-1-6-1 (Heugjinju/Suwon 425) having a high cyanidin 3-glucoside (C3G) contents and ‘Daeribbyeo 1’ having a large grain size. During selected by pedigree breeding method until F10 generation, a promising line, SR28721-7-5-2-1-2-1, was advanced and designated as the name of ‘KNOU 6’. This variety headed on Aug. 30, so 125 days to heading after sowing and has 106 cm culm length. The panicle length of ‘Superjami 2’ was 20.4 cm. The number of panicle per hill was 8.5 ea, and the number of grain per panicles was 143.0 ea. The ratio of fertility of ‘Superjami 2’ was about 82.0% and 1,000-grain weight was about 30.1 g. The 1,000 grain weight of ‘Superjami 2’ (30.1 g) was heavier than that of ‘Heugjinju’. Also, ‘Superjami 2’ has 9 times higher C3G content compared with ‘Heugjinju’.
눈이 크고 C3G색소 고함유 품종 “큰눈자미” 기능성 쌀의 이화학적 특성
함태호,류수노,권순욱 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
흑자색 현미의 기능성 천연색소 C3G 함량을 증대시키고 영양가치가 우수한 거대배 특성을 결합한 큰눈자미(국립종자원 품종등록 : 제4152호, 2012. 10. 17)의 이화학적 특성을 밝혀 기능성 식품소재로서의 활용 가치를 확대하기 위해 수행하였다. 종피의 C3G 함량은 C3G 간이검량법으로 분석하였다(Ryu et al., 1998). 열량 및 탄수화물 함량은 식품공전 계산법으로 분석하였고, 지방 함량은 식품공전 에테르추출법, 회분함량은 식품공전 회분시험법으로 분석하였다(Food code, 2000). 단백질은 Kjedahl법으로, 양이온 함량은 ICP-AES측정법으로 측정하였다. 조사한 결과를 요약하면 다음과 같다. 현미천립중은 18.9g 정도이고, 장폭비는 2.14로 중원형이며, 현미에서 쌀눈의 비율이 8.2% 수준으로 흑진주벼의 2.5배 정도이며, 현미 1립 기준 쌀눈의 무게가 흑진주벼의 2.8배 수준인 거대배아미 품종이다. 현미의 지방함량과 Lysine 함량이 흑진주벼 보다 높고, 종피의 안토시아닌 주색소인 C3G 함량이 흑진주벼에 비해 2배 정도 높은 품종으로 건강기능성 소재로서 활용가치가 높을 것으로 기대된다.