유전자조작, 균주분리 재래식 된장으로부터 혈전용해활성을 나타내는 세균의 분리 및 동정

현광욱 ( Kwang Wook Hyun ),이종수 ( Jong Soo Lee ),함정희 ( Jung Hee Ham ),최신양 ( Shin Yang Choi ) 한국미생물생명공학회 2005 한국미생물·생명공학회지 Vol.33 No.1

입원중 피부과로 의뢰된 환자의 통계분석 ( 96년-99년 )

김문정,함정희,조소연,황규광 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.8

Background: Dermatologic practice occurs mainly in the outpatient setting. The reasons for, frequency, and impact of inpatient dermatologic consultation may be quite different from outpatient practice and there has been a lot of changes in dermatologic consultation recently. Several studies about characteristics of dermatologic consultation have been reported. Objectives : The purpose of this study was to examine the characteristics of inpatient dermatologic consultation in Ewha womans university hospital for 3 years and also to compare the result of the report in 1991. Methods: During a period of 3 years (1996.2-1998.3), we prospectively recorded the demographics of the patients for whom consultation was requested, the final diagnosis of the dermatologic service, and the tests necessary to arrive at diagnosis, and reasons for dermatologic consultation. Results : 1) The consulted patients were 1019 among 38,402 inpatients (2.7%). 2) The total number of male and female patients were 547(53.7%) and 472(46.3%), respectively (M:F = 1.2:1). 3) The most frequent age group was the 6th decade(18.4%) and the highest incidence of seasonal distribution was spring. 4) The patients in internal medicine were the most frequently consulted. 5) The most common dermatoses were eczema group, followed by drug eruption, fungal infection, viral infection, acne vulgaris, urticaria, etc. 6) Distribution of dermatoses according to seasons and individual departments was statistically different in various dermatoses. 7) Frequent causes of consultation were as follows: the dermatologic diseases or conditions 68.3%, the diseases related to treatment 12.3%, the skin lesions of systemic diseases 10.0%, the dermatologic tests 3.5%, and admission to other departments with dermatologic diseases 1.9%, respectively. 8) The rate of consultation for drug eruption was increased markedly compared to that of new patients at OPD in 1998. Conclusions: The characteristics of inpatient dermatologic consultation in our hospital have been changed and furthermore the characteristics were quite different from that of oupatient practice.

비전형적인 양상을 보인 각질하 농포성 피부증 1 예

김연진,함정희,조소연,황규광 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.7

Subcorneal pustular dermatosis(SPD) or Sneddon-Wilkinson disease is a chronic, relapsing, pustular eruption generally involving the intertriginous area and affecting mainly elderly women. We report a case of SPD presenting in a 24-year-old female with erythematous circinate annular peripheral scaly patches on her right thigh. Histopathologically, the subcorneal pustules contained numerous neutrophils, with only a few eosinophils. Underlying stratum malpighii was slightly edematous and contained small numbers of neutrophils. No acantholytic cells or spongiosis were found. The patient was treated by dapsone successfully.

인설성 수부습진 병변의 각질층 세라마이드와 유리 아미노산

이미애,함정희 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.7

㎍Background: Lipids of horny layer forming multiple lamellar structure in the intercellular space acts as a skin barrier having a primary protective function and keeps softness and flexibility of the skin by absorbing and maintaining moistures. Among the intercellular lipids, ceramides mainly contribute to this important role. In xerotic eczema, atopic dermatitis, psoriasis, ichthyosis, and experimentally induced scaly lesion showing dryness and scales, the amount of ceramides is decreased or distribution of ceramide is changed. In addition to lipids, free amino acids, a component of NMFs, serve as a water retainer and are decreased in ichthyosis or experimentally induced scaly lesion. Hand eczema has dry and scaly lesion showing impaired skin barrier and low water content. So, changes of ceramides or free amino acids can be considered in the lesion of hand eczema. Objective: The purpose of this study was to confirm the relationship between the development of hand eczema and changes of ceramides or free amino acids. Method?The lipids and free amino acids in scales from lesion of hand eczema were analyzed by using high performance thin layer chromatography and amino acid analyzer. Results: Amounts of total lipids extracted were 0.63±0.33㎍/㎠ in hand eczema and 0.44±0.26 ㎍/㎠ in control. There was no difference between the two groups. Cholesterol sulfate, glucosyl ceramide, cholesterol, triglyceride, sterol ester, and n-alkane showed no difference between hand eczema and control. But ceramides were significantly decreased in hand eczema(11.0±5.5%) compared with control(21.4±8.0%)(p$lt;0.05). Especially, ceramide type IV was significantly decreased in hand eczema (6.6±5.3%) compared with control(15.6±6.2%)(p$lt;0.05) but ceramide type III in hand eczema did not differ from control. Amounts of total free amino acids in 10mg of scale were 10.4±3.1nmol in hand eczema and 9.5±3.0nmol in control. There was no significant difference between the two groups. Conclusion: Dry skin and scales in hand eczema are related to the decrease of total amount of ceramides and ceramide type IV than amino acids in horny layer. To clarify the exact pathogenesis of hand eczema, further investigations on all types of ceramides and their defect in the process on biosynthesis of ceramides will be necessary.

AIDS 연관형 Kaposi 육종 1예

김지현,김성현,함정희,조소연,황규광 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.8

We report a case of AIDS-related Kaposi's sarcoma in a 65-year-old male. He developed many asymptomatic purplish colored patches and nodules on his four extremities and anterior chest with edema of both legs for 2 months. The ELISA for the anti-HIV antibody was positive and it was confirmed by Western blot. The histologic finding showed abnormally proliferated and dilated vessels, vascular slits, spindle-shaped cells and extravasated erythrocytes in the dermis. In immunohist-ochemical study, the cryptic vessels were positive with CD31. Human herpesvirus-8 was detected by PCR.

장기간 전신 광화학요법 시행받은 백납 환자에서 발생한 PUVA 각화증과 각화극세포종 1 예

김성현,강민정,함정희,조소연,황규광 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.9

Systemic PUVA therapy can produce various acute reactions and potential long-term damage including benign and malignant skin tumors. Obviously the risk is related to DNA damage, but PUVA-induced down-regulation of immune responses may play an additional role. Keratoacanthoma is etiologically related to sunlight and immunosuppression. PUVA keratoses are raised warty papules with a broad base and a diameter of several millimeters to 1 centimeter, and they are associated with an increased risk of nonmelanoma skin cancer. We report a case of solitary keratoacanthoma and multiple PUVA keratoses all developing in vitiliginous areas in a patient receiving long-term, high-dose PUVA therapy for generalized vitiligo. A 57-year-old Korean female, who had undergone intermittent systemic PUVA therapy for 11 years, noted multiple asymptomatic, yellowish, hyperkeratotic papules on the dorsa of hands and feet 1 year previously, and a bean-sized raised painful nodule filled with keratin plug on the dorsum of right hand 1 month previously. Skin biopsy confirmed the diagnoses of PUVA keratoses and keratoacanthoma, respectively. PCR-SSCP analysis revealed no mutation of p53 tumor suppressor gene in this case.

전기소작술 후 발생한 Tufted Angioma 1 예

김현주,신나라,함정희,조소연,황규광 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.7

Tufted angioma is a benign, slowly progressive angioma with typical histologic findings of small, circumscribed angiomatous tufts and lobules scattered in the dermis. More than 50% of cases occur during the first year of life, but rarely it develops late in life. There was only one reported case of tufted angioma after trauma in the English literature. We report a case of tufted angioma, which developed after electrocautery in a 61-year-old woman showing histologic features of typical tufted angioma.