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견적혈구(犬赤血球)에 감염(感染)된 Babesia gibsoni의 미세구조(微細構造)에 관한 연구(硏究)
한재철,이주묵,채준석,윤창모,Han, Jae-Cheol,Lee, Joo-Muk,Chae, Joon-Seok,Yoon, Chang-Mo 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.1
For the ultrastructural observation on Babesia gibsoni(B gibsoni), the protozoa were challenged experimentally to splectomized dog. To examine the ultrastructure of the B gibsoni in the erythrocyte, the infected erythrocytes were collected at the cephalic or jugular vein of the dog. The results obtained by TEM(transmission electron microscopy) were as follows; 1. The sizes of protozoa in erythrocytes are $0.92{\pm}0.36{\mu}m{\times}0.67{\pm}0.21{\mu}m$, the sizes of nucleus of the protozoa are $0.55{\pm}0.24{\mu}m{\times}0.38{\pm}0.26{\mu}m$, and sizes of rhoptries in plasma of the protozoa are $0.33{\pm}0.05{\mu}m{\times}0.25{\pm}0.07{\mu}m$, respectively. 2. The tropozoite membrane in the erythrocyte was one, and it's nuclear membrane was made up of double. But the protozoa of initial stage in infected erythrocyte had double clear mambranes, and distinguished from plasma membrane of red blood cell. 3. The mitochondrialike structures covered with two membranes were observed in the protozoa. 4. Mitochondria and vesicles of the reticulocyte were observed near protozoa in the erythrocyte. 5. There are rhoptry, coiled structure and single nucleous in the merozoite. 6. The shape of rhoptry was round or ovoid form and in occasionally, the content of rhoptry was lost partially. 7. There was able to observe the dividing process of the protozoa. 8. Maurer's cleft-like structure was observed.
토끼 간콕시듐(Eimeria stiedae) 감염 증례
한재철 ( Jae Cheol Han ),한규삼 ( Kyu Sam Han ),이성희 ( Sung Hee Rhee ),마쓰다기꾸 ( Kiku Matuda ),임병무 ( Byung Moo Rim ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
Hepatic coccidiosis was occurred in a rabbit farm in Chonbuk province. Clinically, rabbits showed anorexia, diarrhea, dehydration, and depression, subsequently died 3-5 days after onset of clinical signs. Grossly, multifocal white spots or lines on the liver surface were observed. Histopathologic lesions included hyperplasia of bile duct epithelium with infiltration of inflammatory cells such as plasma cells and granulocytes, which represents chronic pericholangitis. Different developmental stages of Eimeria stiedae were observed inside the epithelium of biliary system. This is the case of hepatic coccidiosis in rabbits.
양식넙치(Paralichthys olivaceus)의 복수증에 관한 연구 -Edwardsiella tarda의 일부 특성과 병원성 및 대책-
한재철 ( Jae Cheol Han ),김영진 ( Young Jin Kim ),서형석 ( Heyng Seok Seo ),김영길 ( Young Gill Kim ),이근광 ( Keun Kwang Lee ),안병목 ( Pyoung Mok Ahn ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1
Characteristics, pathogenicity and control of the causative organisms isolated from diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KEF-1) with l0×l07cfu/fish were died within 60 hrs, but 0~9 flounders infected group with l.0×106 cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with 1.0×l07cfu/fish were died within 36 hrs, while 0~7 flounders infected with l0×l06cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-l strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-l strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.
TaqMan 실시간 중합 효소 연쇄반응에 의한 살모넬라속의 검출 및 ompC 항원단백 유전자의 비교
이영성,최경성,김명철,한재철,채준석,Lee, Young-Sung,Choi, Kyoung-Seong,Kim, Myeong-Chul,Han, Jae-Cheol,Chae, Joon-Seok 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.4
Antigenic ompC genes of S. gallinarum, S. pullorum and S. dublin were characterized among Salmonella spp. isolated from chickens and other animals to identify genetic variation. Salmonella ompC gene fragment (1,027 bp) was amplified by PCR and the amplicons were cloned for comparison of nucleotide sequences. The identity of the sequences between S. gallinarum and S. pullorum, S. gallinarum and S. dublin, S. pullorum and S. dublin was 99.8%, 97.6% and 97.8%, respectively. Also, we found that ompC has some diversity between S. gallinarum and S. pullorum, and other Salmonella spp. which may be useful to type the organisms. Similar to diagnosis in other organisms, the TaqMan PCR method can be applied to rapid and accurate diagnosis of salmonellosis in chickens and other animals. We designed PCR primers and TaqMan probe for flagellin gene (fliC) for detection of Salmonella spp. by TaqMan PCR. The TaqMan PCR method was 10,000 times more sensitive than conventional PCR.
고원석 ( Won Seok Koh ),이재욱 ( Jae Woog Lee ),임정철 ( Jeong Cheol Im ),한재철 ( Jae Cheol Han ),이희문 ( Hee Mun Lee ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.2
In order to monitor the parasites, 204 fecal samples were taken from Korean indigenous goats of Iksan-branch. Then identification of the parasites was determined by the fecal examination using the floatation and microscopical examination, respectively. The detection of rates was 91.2%, and mixed infection rates were single 38.7%, double 28.4%, triple 15.2%, Quadraple 6.9% and Qunituple 20.0%. The isolated were identified as Eimeria spp from 169 heads, Strongyloides papillosus from 56 heads, Ostertagia spp from 24 heads, Trichostrongylus spp from 22 heads, Moniezia expensa from 18 heads, Oesophagostomum spp from 17 heads, Bonostomum spp from 12 heads, Cooperia spp from 12 heads, Heamonchus spp from 8 heads and Capillaria spp from 2 heads.
TaqMan 실시간 PCR법에 의한 개 전염성 간염 바이러스의 검출
왕혜영,최재용,이미진,박진호,조매림,한재철,최경성,채준석,Wang, Hye-young,Choi, Jae-yong,Lee, Mi-jin,Park, Jin-ho,Cho, Mae-Rim,Han, Jae-cheol,Choi, Kyoung-seong,Chae, Joon-seok 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
The aim of this work was the validation of a rapid real-time PCR assay based on TaqMan technology for the unequivocal identification of infectious canine hepatitis (ICH) virus, to be used directly on DNA purified from blood specimens. A real-time PCR system targeting at the E3 ORFA gene sequence of canine adenovirus type 1 was optimized and validated through comparative analysis of samples using conventional PCR system. The real-time PCR assay based on TaqMan technology could disclose 23 (37.7%) out of 61 samples as PCR positive. In contrast, 18 (29.5%) samples were found PCR positive when conventional PCR was applied on these samples. The use of the ABI Prism 7700 sequence detection system allowed the efficient determination of the amplified product accumulation through a fluorogenic probe. The entire real-time TaqMan PCR assay, including DNA extraction, amplification, and detection could be completed within 3 hours. The detection method of real-time TaqMan PCR assay was 1,000 times more sensitive than conventional PCR. Real-time TaqMan probe and primer set developed and optimized in this study is a sensitive, rapid and accurate method for detection of ICH virus and can be effective screening tool for the detection of ICH in a diagnostic laboratory routines.
RT-PCR과 ELISA를 이용한 PRRS 진단 및 항체가 조사
추금숙 ( Keum Suk Chu ),한규삼 ( Keu Sam Han ),한재철 ( Jae Cheol Han ),송희종 ( Hee Jong Song ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1.Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: 95℃ for 30 sec, 45℃ for 30 sec, and 72℃ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were 6.6%, 53.3% and breeding farms 65%, 65% and 38.7%, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average 21% in domestic farms, 56.2% in breeding farms, and 29.9% in AI center.
추금숙 ( Keum Suk Chu ),조현웅 ( Hyun Ung Cho ),한재철 ( Jae Cheol Han ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2
The strategy for infectious bursal disease (IBD) control and its success rate under field conditions depends on hygiene management, IBD field pressure, level, and variation in maternally derived IBD antibodies. This study investigated the level of IBD-specific antibody by ELISA and the prevalence of IBD virus by PCR in broilers, white-semi broilers, and Korean native chickens raised in Jeongeup, Jeonbuk. IBD-specific maternally derived antibodies were measured from 698 chickens and the mean titers of maternal antibodies were 3,572±1,402 in broilers, 1,262±762 in white-semi broilers, and 1,932±912 in Korean native chickens. At 2 weeks after vaccination, the geometric mean antibody titers of broiler, white-semi broiler, and Korean native chicken were 582±427, 3255±1,080, and 1,023±499, respectively. According to sequence analysis of the variable virion protein 2 gene, 4 isolates were found to be very virulent IBDV, 9 isolates classical virulent, and 2 isolates intermediate plus vaccine strain.