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RT-PCR과 ELISA를 이용한 PRRS 진단 및 항체가 조사
추금숙 ( Keum Suk Chu ),한규삼 ( Keu Sam Han ),한재철 ( Jae Cheol Han ),송희종 ( Hee Jong Song ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3
The studies were performed for the PRRS antigen and antibody detection from breeding farms, artificial insemination(AI) center and growing farms in Jeonbuk province. 1.Specific PRRS primers were successfully amplified ORF6 617bp and ORF7 448bp on agarose gel. 2. RT-PCR method has been establish by commercial kit and the thermal cycler program consisted of 30 cycles: 95℃ for 30 sec, 45℃ for 30 sec, and 72℃ for 45 sec. 3. The results of PRRS antibody test by ELISA method in AI centers were 6.6%, 53.3% and breeding farms 65%, 65% and 38.7%, respectively. The serological positive of the antibody in gilt higher than sow. 4. The sero-positive of the PRRS antibody showed average 21% in domestic farms, 56.2% in breeding farms, and 29.9% in AI center.