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서형석 ( Heyng Seok Seo ),임정철 ( Jung Chul Lim ),허부홍 ( Boo Hong Hur ),권정택 ( Jung Taek Kwon ),김성문 ( Seong Moon Kim ),천희웅 ( Hee Woong Chun ),최인방 ( In Bang Choi ),김진상 ( Jin Shang Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
The purposes of this study were to characterize the disposition of sulfathiazole(ST) and to investigate the effects of sodium bicarbonate on the disposition of ST in broiler chicks(2.5~3.0kg). Animals were given ST acutely(10~80mg/kg, PO), and plasma, kidney, muscle, heart, liver and spleen samples were collected and analyzed for ST by high performance liquid chromatography. The plasma and tissue data was consistent with a one-compartment pharmacokinetic model. The drug is rapidly but incompletely(2.5~3.87%) absorbed with peak plasma and tissue levels being achieved within one hour after dosing. The plasma and tissue levels depended on drug dosage, and the descending order in concentration of ST was kidney>plasma>heart>muscle≥spleen≥liver from animals sacrificed at one hour after dosing. Moreover, significant positive correlations(r>0.9) existed between plasma and tissue levels of ST. In addition, sodium bicarbonate pretreatment decreased plasma level, indicating that an alkalinization stimulate the excretion of ST. Results of this study suggest that oral application of ST was rapidly absorbed and eliminated, and confirmed that tissue residues of ST can be estimated from plasma drug concentration in broiler chicks.
To investigate the species and infection rate of Tripartiella sp in three species of freshwater fish, carp(Cyprinus carpio), goldfish (Crassius auratus) and Korean catfish(Parasilurus asotus), fish cultured in the fish farms next to Yosu and in the western area of Chunbuk province in Korea was examined from April to June, 1996. The infection rate was evaluated after observing the gill and mucous from 130 fish under the microscope. The species of Tripartiella was identified on the basis of the shape and number of denticle according to methods of Bychowsky(1985) after staining the parasites with 2% methy-ene blue solution. All the detected-parasite was classified as Tripartiella differed from Trichodina reported previously in our country. T carassii, T californica, T rhombi from Goldfish, T rhombi from common carp. T californica from Israeli carp and T bychowsky from Korean catfish were observed. In the fish from the fish farms in the western area of Chunbuk province, the infection rate of Tripartiella sp was ranged from 6.7% to 100%, but in neither carp nor goldfish from the farms next to Yosu the parasite detected.
백귀정 ( Kui Jeong Baek ),서형석 ( Heyng Seok Seo ),노영선 ( Young Sun Roh ),양해동 ( Hae Dong Yang ),허부홍 ( Boo Hong Hur ),서이원 ( Lee Won Seo ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
Plasmids are covalently closed circular molecules of DNA that are stably inherited and replicate somewhat independently of the bacterial chromosome. Genes carried on plasmids can mediate a wide variety of important functions, including antibiotics(R plasmids) and heavy metals resistance, toxins production, cell penetration, iron chelation, complement resistance, and metabolic characteristics such as sucrose and lactose fermentation. Fifty strains of lactobacilli were isolated from 26 staters and 29 fermented milk products. They were classified 27 strains as Lactobacillus paracasei subsp paracasei , 11 stains as Lactococcus lactis subsp cremoris, 6 strains as L delbrueckii subsp lactis, 4 strains as L acidophius, and 2 strains as L delbrueckii subsp bulgaricus. All of these strains were examined for drug resistance and transferability of R plasmids. All of the isolates were sensitive to Am, C, CF, E, NB, P, T, and Te. But resistant to SXT 94%(47 strains), K 66%(33 strains), S 56%(28 strains), ENR 50%(25 strains), NOR 38% (19 strains) CIP 38%(19 strains), GM 16%(8 strains), and N 14%(7 strains), in order. And 32 different resistant patterns were found. The most frequently encountered patterns were CIP-ENR-K-NOR-S-SXT (5 strains). In vitro R plasmids transfer experiment, 57 antibiotic resistant strains which were not trans-fer to the recipient 2 Escherichia coli strains by conjugation. These results indicate that Lactobacillus in internal trade market` stater recognize R factor but transmissible R plasmid is not existed.
In order to develop a good separation and simultaneous analysis of different sugar in an artificial mixed sugar solution, we analyzed 10 sugar components in an artificial mixed sugar solution composed of fructose, glucose, mannitol, sucrose, maltose, lactose, xylose, xylitol, erythritol, and trehalose with using HPLC-ELSD or HPLCRI. Separation and Quantification by I-IPLC-ELSL) was superior to those by 1-IPLC-R1 and detection sensitivity by HPLC-ELSD was higher then that by HPLC-RI as microgram(Ltg) level. 1. The units of minimal detectable limits were showed pg/me and ng/m by the HPLC-RI and I-IFLC-ELSD, respectively. 2. The condition of ELSD was drift tube temperature 82 C, N2 gas flow rate 2.10 SLPM, and column oven temperature 30 `C, respectively. Isolation and recovery rates of single sugar from the multiple sugar solution was higher at the condition (time: flow rate: D.W.: ACN MeOR, mm: ni/min: v: v: v) of linear gradient elution of mobile phase as 0: 1.00:15: 85: 0,1:1.00:6:90:4, 17:1.00:10:70:20, 23:1.00:15:85:0 and 35: 1.00:15:85:0, in order.
Recently, mass spectrometry coupled with liquid chromatography(LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra C18 column with 0.01% trifluoroacetic acid-acetonitrile(TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with 50% acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via [M+H]+ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles
Characteristics, pathogenicity and control of the causative organisms isolated from diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KEF-1) with l0×l07cfu/fish were died within 60 hrs, but 0~9 flounders infected group with l.0×106 cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with 1.0×l07cfu/fish were died within 36 hrs, while 0~7 flounders infected with l0×l06cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-l strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-l strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.
A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics(AMGs) using Heptafluorobutyric acid(HFBA) as a ion-pairing reagent. AMGs(amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry(ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.
Porcine transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and rotaviruses are considered as the most important causative agents of diarrhea in piglets. The study established 3 method vaccination programs to prevent PEDV. A (LL)group inoculated twice vaccinations on 2-weeks-interval during the late term of pregnant sows with PEDV live vaccine. The B (LKK) group was applied that one time single PEDV live vaccine at the pre-mate followed by the TGEV·PEDV combined inactivated vaccine (twice vaccination on 2-weeks interval at the third-trimester). C (KK) group was applied to sow which inoculated twice vaccination on 2-weeks-interval during the late term of pregnant sows with by the TGEV, PEDV combined inactivated vaccine. As the result of SN test on sows in the pig farm before vaccination, antibody titers was showed 9/45 (20.0%). By comparison with the serum neutralizing antibody titers against PEDV of the vaccination programs after PEDV of the vaccination, A group and B group vaccination method was higher than those of C group in sows. In the piglets up to 2 weeks of age, A group was showed antibody titers of 17/22 (81.8%) that showed 2-128, and B group was showed antibody titers of 30/37 (81.1%) that showed 2-512, and C group was showed antibody titers of 14/28 (50.0%) that showed 2-32. On the other hand, PEDV antibody titers were tested for the survey. As the results of SN test, Aujeszky`s disease survey in 54 pig farms from november 2005 to august 2006, antibody titers of 47/286 (16.4%) showed above 2. Five breeding farms were antibody titers of 38/77 (49.4%), Wanggung zone farms antibody titers of 59/85 (69.4%). In pigs farms vaccinated the first of twice PEDV live vaccine, and after 6 month, the second of twice TGEV·PEDV combined inactivated vaccine (LLKK, 256-1024 titer) method was higher than those of vaccinated twice the early term of pregnant, and twice the late term of pregnant sows of PEDV live vaccine (LLLL, 32 titer).