황체막에서의 $Ca^{++}-ATPase$의 특성

최규복,구본숙,김인교,Choi, Gyu-Bog,Koo, Bon-Sook,Kim, In-Kyo 대한생리학회 1986 대한생리학회지 Vol.20 No.2

It has been reported that the luteal function may be regulated by the intracellular calcium in luteal cells (Higuchi et al, 1976; Dorflinger et at, 1984; Gore and Behrman, 1984) which is adjusted partially by $Ca^{++}-ATPase$ activities in luteal cell membranes (Verma and Pennistion, 1981). However, the physicochemical and kinetic properties of $Ca^{++}-ATPase$ in luteal membranes were not fully characterized. This study was, therefore, undertaken to partially characterize the physicochemical and kinetic properties of $Ca^{++}-ATPase$ system in luteal membranes and microsomal fractions, known as an one of the major $Ca^{++}$ storge sites (Moore and Pastan, 1978), from the highly luteinized ovary Highly luteinized ovaries were obtained from PMSG-hCG injected immautre female rats. Light membrane and heavy membrane fractions and microsomal fractions were prepared by the differential and discontinuous sucrose density gradient centrifugation method desribed by Bramley and Ryan (1980). Light membrane and heavy membrane fractions and microsomal fractions from highly luteinized ovaries are composed of the two different kinds of $Ca^{++}-ATPase$ system. One is the high affinity $Ca^{++}-ATPase$ which is activated in low $Ca^{++}$ concentration (Km, 10-30 nM), the other is low affinity $Ca^{++}-ATPase$ activated in higher $Ca^{++}$ concentration $(K_{1/2},\;40\;{\mu}M)$. At certain $Ca^{++}$ concentrations, activities of high and low affinity $Ca^{++}-ATPase$ are the highest in light membrane fractions and are the lowest in microsomal fractions. It appeares that high affinity $Ca^{++}-ATPase$ system have 2 binding sites for ATP (Hill's coefficient; around 2 in all membrane fractions measured) and the positive cooperativity of ATP bindings obviously existed in each membrane fractions. The optimum pH for high affinity $Ca^{++}-ATPase$ activation is around S in all membrane fractions measured. The lipid phase transition temperature measured by Arrhenius plots of high affinity $Ca^{++}-ATPase$ activity is around $25^{\circ}C$. The activation energies of high affinity $Ca^{++}-ATPase$ below the transition temperature are similar in each membrane fractions, but at the above transition temperature, it is the hightest in heavy membrane fractions and the lowest in microsomal fractions. According to the above results, it is suggested that intracellular $Ca^{++}$ level, which may regulate the luteal function, may be adjusted primarily by the high affinity $Ca^{++}-ATPase$ system activated in intracellular $Ca^{++}$ concentration range $(below\;0.1\;{\mu}M)$.

고혈압 환자의 혈장 Endothelin 농도

최규복(Gyu Bog Choi),김성남(Seong Nam Kim),윤견일(Kyun Ill Yoon) 대한내과학회 1995 대한내과학회지 Vol.48 No.3

N/A Objectives: Following the discovery in 1988 of Endothelin by Yanagisawa, three types of Endothelin called Endothelin-l, Endothelin-2 and Endothelin-3 were identified. Among them, Endothelin-1 is secret- ed from vascular endothelial cells. Endothelin-1 has been shown to be a potent vasoconstrictor in isolated human vessels as well as having positive inotropic effects on human atria by a direct action on the heart muscle. Infusion of Endothelin-1 into human volunteers has also shown it to be a constrictor of resistance vessels in vivo. So it is possible that Endothelin-1 plays a role in maintenance of blood pressure, But there is debate on the plasma concentration of Endothelin-1 in hypertensive patients. Therefore, the present study was designed to deter- mine plama Endothelin concentration in hypertensive patients and control subjects. Methods: We measured and analyzed the endothelin concentration in plasma and 24- hours urine by radioimmunoassay in 15 patients with essential hypertension, 5 patients with secondary hypertension, 5 patients with chronic renal failure and 8 control groups. Results: 1) Comparison between Uncontrolled hypertensive patients and Control subjects: The mean level of plasma Endothelin in patients with essential hypertension(22.7±24.27pg/ml) was significantly higher than that in control subjects(4.0±2.9pg/ml; p<0.05). But there was no significant. difference in urine endothelin concentration and endothelin clearance among each groups. 2) Comparison between Controlled hypertensive patients and Control subjects: The mean level of plasma Endothelin in patients with essential hypertension(8.8±9.88pg/ml) seemed to be higher than that in control subjects(4.0±2.69pg/ml), but there was no statistical significance. The mean level of urine Endothelin in patients with secandary hypertension(57.1 + 13,17pg/ml) wns significantly higher than that in control subjects(16.1±9.64pg/ ml; p<0.05) and in patients with essential hypertension(14.2±3.46pg/ml; p<0.05). But there was no significant in endothelin clearance among each groups. Conclusion: In conclusion, it is hardly to say that endothelin has a primary pathophyaiological role in hypertension. Whereas plasma endothelin concen-tration might to be elevated secondarily as a result of endothelial damage from uncontrolled hypertension. But further study will be need about it.

지속적 혈액투석환자에서 Desferrioxamine 투여 후의 혈장 Endothelin 농도 변화

최규복(Gyu Bog Choi),윤견일(Kyun Ill Yoon) 대한내과학회 1996 대한내과학회지 Vol.51 No.3

N/A Objectives: It has been reported that the risk of oxygen radical injury is increased in chronic renal failure due to the decreased endogenous serum antioxidants. Especially, the C5a induced by membrane bioincompatibility can stimulates neutrophils to release of oxygen free radicals, resulting in endothelial cell injury. However, Desferrioxamine(DFO) act as an iron chelator, which blocks iron-catalyzed Haber-Weiss reaction and inhibits release of oxygen free radicals from activated neutrophils. It is also reported that endothelin(ET) can be released from endothelial cells in response to vascular damage such as atherosclerosis. Therefore, we administered DFO, into maintenance hemodialyein patients. Then we examined the possibility of oxygen radical injury during interdialytic period and its relation with the plasma ET concentration. Methods: During the last 1-2 hours of hemodialysis, DFO(40mg/kg in 5% D/W 200cc) was infused intravenously into 13 patients(DFO group), and placebo(5% D/W 200cc only) was infused with same manner into 9 patients(Placebo group). We sampled blood for measurement of plasma ET concentration just before the initiation of hemodialysis on the day of infusion, on the 2nd-3rd day and on the 7th day after infusion. Also, we examined 26 non-diabetic patients with normal renal function as a norma1 control. Results: The mean plasma ET concentration in total hemodialysis patients is higher (5.08±3.09pg/ ml) than in normal control (2.58±1.08pg/ml, p<0.01). There was no statistical difference between two hemodialysis groups in plasma ET concentration measured before infusion (5.56±3.50pg/ml in DFO group, 4.38±2.40 pg/ml in placeb group). In DFO group, plasma ET concentration decreased significantly on the 2nd-3rd day (3,49±2.08pg/ml, p<0.01), but increased significantly on the 7th day (5.62± 2.95pg/ml, p<0.05), In contrast, there were no significant changes in plasma ET concentration in placebo group. There was no significant difference in the decrement of plasma ET between the cases of transferrin saturation below and above 60% and there was no relation between the plasma ET decrement and transferrin saturation or serum ferritin in DFO group. Conclusion: The decrease of plasma ET concentration after DFO infusion might be the result of diminished endothelial cell injury from oxygen free radicals. Therefore, we believe that the oxygen radical injury can occur during not only the hemodialysis but also the interdialytic period. Also these results suggest that the oxidant damage of endothelial cell may be one of the causes of elevated ET concentration in chronic renal failure, However, we could not confirm in this study whether the obtained results were caused by the chronic effects of membrane bioincompatibility or by the decreased endogenous serum antioxidants.

황체막에서의 Ca⁺⁺-ATPase의 특성

최규복(Choi, Gyu-Bog),구본숙(Koo, Bon-Sook),김인교(Kim, In-Kyo) 대한생리학회 1986 대한생리학회지 Vol.20 No.2

It has been reported that the luteal function may be regulated by the intracellular calcium in luteal cells (Higuchi et al, 1976; Dorflinger et at, 1984; Gore and Behrman, 1984) which is adjusted partially by Ca⁺⁺-ATPase activities in luteal cell membranes (Verma and Pennistion, 1981). However, the physicochemical and kinetic properties of Ca⁺⁺-ATPase in luteal membranes were not fully characterized. This study was, therefore, undertaken to partially characterize the physicochemical and kinetic properties of Ca⁺⁺-ATPase system in luteal membranes and microsomal fractions, known as an one of the major Ca⁺⁺ storge sites (Moore and Pastan, 1978), from the highly luteinized ovary Highly luteinized ovaries were obtained from PMSG-hCG injected immautre female rats. Light membrane and heavy membrane fractions and microsomal fractions were prepared by the differential and discontinuous sucrose density gradient centrifugation method desribed by Bramley and Ryan (1980). Light membrane and heavy membrane fractions and microsomal fractions from highly luteinized ovaries are composed of the two different kinds of Ca⁺⁺-ATPase system. One is the high affinity Ca⁺⁺-ATPase which is activated in low Ca⁺⁺ concentration (Km, 10-30 nM), the other is low affinity Ca⁺⁺-ATPase activated in higher Ca⁺⁺ concentration (K_½, 40 μM). At certain Ca⁺⁺ concentrations, activities of high and low affinity Ca⁺⁺-ATPase are the highest in light membrane fractions and are the lowest in microsomal fractions. It appeares that high affinity Ca⁺⁺-ATPase system have 2 binding sites for ATP (Hill s coefficient; around 2 in all membrane fractions measured) and the positive cooperativity of ATP bindings obviously existed in each membrane fractions. The optimum pH for high affinity Ca⁺⁺-ATPase activation is around S in all membrane fractions measured. The lipid phase transition temperature measured by Arrhenius plots of high affinity Ca⁺⁺-ATPase activity is around 25℃. The activation energies of high affinity Ca⁺⁺-ATPase below the transition temperature are similar in each membrane fractions, but at the above transition temperature, it is the hightest in heavy membrane fractions and the lowest in microsomal fractions. According to the above results, it is suggested that intracellular Ca⁺⁺ level, which may regulate the luteal function, may be adjusted primarily by the high affinity Ca⁺⁺-ATPase system activated in intracellular Ca⁺⁺ concentration range (below 0.1 μM).

Urea Kinetic Modeling 을 이용한 지속적 혈액투석 환자의 영양 상태 평가

최규복(Gyu Bog Choi),변정란(Jung Lan Byun),박정은(Jeong Eun Park),이은영(Eun Young Lee),이지수(Ji Soo Lee),편욱범(Wook Bum Pyun),고영엽(Young Youp Koh),윤견일(Kyun Ill Yoon) 대한내과학회 1994 대한내과학회지 Vol.46 No.2

N/A Objectives: Nutritional factors play a role in the morbidity and mortality of patients in maintenance hemodialysis as well as in their quality of life and ultimate rehabilitative potential. It was reported that if the deviation of urea distribution volume calculated by Urea Kinetic Modeling(UKM) (Vol-Dev) from Anthropometric volume exceeded the acceptable range, clinical application of the NPCR (normalized protein catabolic rate) as nutritional index might be inappropriate. And it was also reported that if the KT/Vurea without consideration of residual renal function (D-KT/V) was above 1.5 or below 0.8, the NPCR might be inaccurate. So we selected patients whose Vol-Dev was within the acceptable range and grouped according to the D-KT/V. Then we analyzed the relationship between the NPCR and other nutritional parameters. Methods: We selected 17patients undergoing maintenance hemodialysis with adequate Vol-Dev level and grouped as group 1 if D-KT/V was between 0.8 and 1. 5, as group 2 if D-KT/V was below 0.8 or over l.5, We measured the mean level of albumin, calcium, phosphorus and hematocrit and calculated midarm muscle area (MAMA), midarm fat area (MAFA) as anthropometric measurements. Results: 1) Nutritional Index: There were no differences in serum albumin, calcium, phosphorus and hematocrit between two groups. The mean MAMA of group 1 (37.4cm2) was not different from that of group 2(27.9cm2), but mean MAFA of group 1(19.2cm2) was significantly higher than that of group 2(14.3cm2). The NPCR of group 1 (1.00) was not different from that of group 2(1.12). 2) UKM Parameter: The mean level of D-KT/V as single dialysis dose in group 1 (1.23) was significantly lower than that of group 2(1.69) and the mean level of TW-KT/V as weekly dialysis dose in Group 1 (3.17) was significantly lower than that of Group 2(4.05). The mean level of TWR-KT/V as weekly dialysis dose with consideration of residual renal function in Group 1(3.24) was significantly lower than that of Group 2(4.07) also. 3) Correlation between NFCR and dialysis dose: There was no correlation between D-KT/V and NPCR in both Group. In group 1, there was positive correlation between NPCR and TW-KT/V or TWR-KT/V. But in group 2, there was no correlation between NPCR and TW-KT/V or TWR-KT/U. 4) Correlation between NPCR and Nutritional Index: There was no correlation between NPCR and serum nutritional index (albumin, calcium, phosphorus, hematocrit). There was also no correlation between NPCR and anthropometric parameter (MAMA, MAFA). Conclusions: It is not likely that the NPCR reflects the protein catabolic rate accurately in case of D-KT/V exceeded adequate level (0.8≤, ≤1.5). Although the protein catabolic rate might be increased due to the effect of dialysis itself, there was no significant change in the nutritional status of patients. Even though the D-KT/V was within the adequate level, it is difficult to evaluate the patients nutritional status with NPCR only.

간경변증 환자 혈장 Endothelin 측정의 임상적 의의

김도영(Doe Young Kim),임석아(Seok Ah Im),심기남(Ki Nam Shim),유승기(Sung Kee Ryu),최희정(Hee Jung Choi),최규복(Gyu Bog Choi),문일환(Il Hwan Moon),윤견일(Kyun Il Yoon) 대한내과학회 1994 대한내과학회지 Vol.47 No.4

N/A Background: Endothelin is a newly discovered 21-aminoacid polypeptide that has been shown to produce marked vascoconstriction. Elevated plasma concentration of endothelin has been reported in patients with acute and chronic kidney failure, cardiogenic shack, hypertension, sepsis, acute myocardial infarction, subarachnoid hemorrhage and Raynaud`s phenomenon, and the reports suggest that endothelin plays a contrib- utnry role in the pathogenesis of those diseases. However, plasma levels of endothelin in patients with liver cirrhosis have rarely been reported. This study was conducted to investigate the clinical significance of plasma endothelin concentrations in patients with liver cirrhosis. Methods: Eleven patients with liver cirrhosis and ten healthy control subjects were included in this study. Plasma and random urine endothelin concentrations were measured by radioimmunoassay. Results: 1) The plasma endothelin concentration was significantly higher in patients with cirrhosis than in control subjects (15.5±5.1pg/ml vs. 3.9±2.4 pg/ml, mean±S. D, p<0.01). The urine endothelin concentration was also significantly higher in patients with cirrhosis than in control subjects (47.9±26.6 pg/ml vs. 15.3±9.2 pg/ ml, p<0. 05). 2) In patients with cirrhosis, the plasma endothelin concentration showed a significant negative correlation with creatinine clearance (r=0.76, p<0.01), and a significant positive correlation with fractional excretion of sodium (FENa) (r=0. 75, p<0. 01). Conclusion: From these results, elevated plasma endothelin may play a contributory role in kidney dysfunction in patients with cirrhosis.

급성 바이러스성 간염에서 담낭벽 비후에 관한 초음파적 고찰

경난호 ( Nan Ho Kyung ),박이갑 ( Lee Gap Park ),이순남 ( Soon Nam Lee ),한경숙 ( Kyung Sook Han ),최규복 ( Gyu Bog Choi ),장일중 ( Il Joong Chang ) 대한내과학회 1985 대한내과학회지 Vol.28 No.6

CAPD 합병증에 관한 연구

최규복,윤견일 梨花女子大學校 醫科大學 醫科學硏究所 1991 EMJ (Ewha medical journal) Vol.14 No.2

Since its first introduction by Popovich in 1976. Continuous Ambulatory Peritoneal Dialysis(CAPD) has estabilished itself as an effective method maintaining the patients with end stage renal disease. But recurrent peritonitis remains the most frequent cause of the CAPD failure. So clinical studies were carried on the 15 patients on CAPD who had been treated from June 1989 to march 1991. The following results were obtained. 1) The incidence of peritonitis was 1.73 episode/patient/year. 2) Peritonitis incidence according to the sex showed 1.93 episode/patient/year with female patients and l.46 episode/patient/year with male patients. 3) Peritonitis incidence according to the underlying disease showed 1.94 episode/patient/year with diabetic patients and 1.45 episode/patient/year with non-diabetic patients. 4) The subjective symptom and sign were as follows; abdominal pain(95%). cloudy dialysate(95%). nausea(55%), abdominal tenderness(95%). decreased dialysate drainage (59%), fever(50%), and peripheral leukocytosis(18%). 5) The positive rate in Gram stain of dialysate drainage fluid was 13.6% and the positive rate in culture was 31.8%. of which staphylococcus was 42.9%. 6) The rate of catheter removal due to peritonitis was 22.7%. The most common cause was persistent peritonitis, which accounted for 60% of cases. And all cases of catheter removal were developed in female patients. 7) The complications related to catheter were as follows ; catheter exit site infection(20%), external cuff extrusion(20%), leakage of dialysate fluid(6.7%) and crack on Tenckhoff catheter(6.7%)

용혈이 동반된 Gilbert증후군 1예

최규복,한경숙,김미경,배윤주,이순남,경난호 梨花女子大學校 醫科大學 醫科學硏究所 1985 EMJ (Ewha medical journal) Vol.8 No.1

Gilbert's Syndrome is the name most frequently used to describe a condition which has been called constitutional heptic dysfunction, familial nonhemolytic jaundice of icterus intermittens juvenilis. It is characterized by a benign, persistent, but vari-able elevation of the plasma unconjugated bilirubin. A 27-year-old man was admitted because of general malaise, anorexia, sore throat and slight icteric sclera. On physical examination, there were mild icteric sclera and slightly injected pharynx. The liver was palpable 1 finger breath BRCM and the spleen was also palpable I finger breadth BLCM. Hematologic studies reve-aled slight anemia with slightly elevated corrected reticulocyte count. Liver func-tion tests were normal except for slightly elevated serum total bilirbin. Histolog-ic findings of the liver showed nonspecific findings of mild cholestasis. Reduction in caloric intake to 300 calrory a day for 72 hours resulted in a significant increase in the plasma bilirubin concentration(especially unconjugated form) in this patient. Now he has no subjective symptoms and leads a normal activity without specific treatment.

활동성 전신성 홍반성 낭창환자에서의 Plasmapheresis를 이용한 치험 1례

고영엽,최규복,윤견일 梨花女子大學校 醫科大學 醫科學硏究所 1990 EMJ (Ewha medical journal) Vol.13 No.4

A patient with severe active systemic lupus erythematosus, who had not responded to conventional therapy, was treated with plasmapheresis and subsequent pulse cyclophosphamide. There was the reduction of anti-ds DNA level was deteced after plasmapheresis in conjunction with the immunosuppressive therapy could be and effective modality of management in patients with rapidly deteriorating SLE who are responding inadequately to the conventional therapy.