연구논문 : 비만청소년의 자기효능감에 관한 연구

차영애 ( Young Ae Cha ) 한국미용학회 2003 한국미용학회지 Vol.9 No.1

청소년들의 여드름 등급 및 관련요인과 관리방법에 관한 연구

김광옥 ( Kwang-ok Kim ),차영애 ( Young-ae Cha ) 한국고등직업교육학회 2000 한국고등직업교육학회논문집 Vol.1 No.1

Acne is a prevalent skin disease in adolescence, which may develop undesirable effects such as leaving scars due to misunderstanding and failure to control. Thus this study was conducted to find out the prevalence, the conditions and status of control for acne, and the impact of acne so that the result could provide the basic information for health education in schools, and for the treatment and control of acne. The data were collected from 1042 students at 3 middle schools and 3 high schools in Pusan for a period from December 1 to December 20, 1998, and were analyzed for general characteristics, characteristics related to acne, the status of acne control by using SPSS/PC+ program. The main results were as follows. 1. The prevalence of the acne for the students in the study was 74.9% and showed significant difference by gender with 81.0% for male students and 67.8% for female students. The degree of acne was mild for 74.3% moderate for 19.0% and severe for 6.7%. 2. The most prevalent region for acne development was the face with 98% for male students and 96.7% for female students, followed by the back 17.8%, the neck 13.7%, the chest 13.3% and the arms or legs 6.8% for the males, and the back 28.1%, the chest 12.5%, the arms or legs 5.1% and the neck 4.8% for the females, respectively. The average of period of having acne was 1.7 year. The most frequent case for worsening of acne condition occurred after sweating for male students(33.1%) and due to stress for female students(40.9%). 3. The most prevalent way to gain the information about acne was through friends for male and female students, followed by newspapers or magazines, family, TV or radio. 4. More prevalent way to control acne was at home than using hospital or clinic, and 32.2% of the students were using the soap for acne. 5. The sensitivity to acne was severe for 19.7% of the female students, and 11.9% of the male students

사람 피부각질세포에서 자외선 B 조사로 유도된 핵형 및 세포주기 변화

차 호(Ho Cha),송인환(In Hwan Song),추영애(Young Ae Choo),박숙경(Sook Kyung Park),이인환(In Hwan Lee) 대한해부학회 2000 Anatomy & Cell Biology Vol.33 No.1

자외선 B 의 조사로 인하여 유전자가 표적이 되어 세포 주기가 변화하고 그후 증식하는 과정을 조사하기 위하여 자외 선 B 200J/m2를 조사한 후 흘림세포계측기 (flowcytometer)에서 CellFit 프로그램으로 세포주기의 각 기를 측정하였다. 주된 세포 주기의 변화는 S기가 길어진 것이었으며 핵형 분석에서는 염색체의 수가 near diploid (43-47)에서 hypotetraploid(80+)로 되었다. 자외선 B를 조사한 후 near diploid (43-47)군과 hypotetraploid (80+)군으로 나누어 세포주기를 측정하였더니 near diploid군에서는 S기의 연장이 특징적이었으며 hypotetraploid군에서는 G0-G1기의 길어짐을 보였으며 세포 배양에서 형질 전형을 보였다. 핵형 분석에서 나타나는 새로운 구조적 변형은 del (5q21), 8p+ 및 t (5 : 8) (q21 : pter)이었다. 이중 del (5q21)이 모든 형질 전형된 세포에서 나타났다. 이상의 결과로 미루어 자외선 B의 조사로 변화하는 세포 주기는 M기를 한번 거치지 않는 [G1-S-(G2-G1)-S]의 과정으로 여겨지며 del (5q21) 자리는 암 억제유전자인 APC 유전자의 위치여서 이 유전자의 소실이 세포배양에서 배양 접시에 붙지 않고 자라는 형질전형의 주된 원인이 되는 것으로 생각된다. 핵의 크기 변화는 세포 주기의 변화로 인한 염색체 수의 변화에서 기인되며 세포의 수적 증가가 늦어지는 것으로 생각된다. To investigate the change of cell cycle by genotoxic stress and rebound proliferation in human keratinocytes, the proportions of cell cycle phases were estimated with challenge of UVB irradiation (200 J/m2). With UVB irradiation cell cycle was estimated by Cell Fit program in Flowcytometer, and main change of the cell cycle was S-phase prolongation. In karyotyping, near diploid number of chromosomes changed to hypoteraploid number. Cell cycle phase was estimated in two groups of cells, near diploid and hypoteraploid. In near dipoid cells, S-phase prolongation was specific phenomenon, while specific G0-G1 phase prolongation was shown in hypoteraploid cells which made transformed foci in culture. The new structural anomalies were del (5q21), 8p+, and t (5 : 8)(q21 : pter). Among them, del (5q21) was found in all transformed hypotetraploid cells. These data suggest that progress of cell cycle could be [G1-S-(G2-G1)-S] by UVB irradiation and deletion of 5q21 has a key role for anchorage independent growth, which is deletion of tumor suppressor gene APC locus. That is one of important mechanisms in keratinocyte transformation by UVB irradiation. With the changes of chromosome number and cell cycle, sizes of nuclei got to bigger by two times and growth rate was delayed.

건강증진을 위한 경락 건강체조 개발에 관한

차영애 한국미용학회 2001 한국미용학회지 Vol.7 No.1

The meridians have high concerned about health. But they are lack of concrete access and apply to it. So l develop healthful gymnastics by using the 14 meridian, the pathway of circulation system which rams our life, and meridian point. Subjective healthiness, satisfaction and health promotion standard of the people for research is investigated as well. This study is for spread the exercise and suggestion of theoretical foundation, From February 20 to April 2 in 2001, among the female students of a certain college in 경남, 28people, a testing group which suit it s propose, aye selected and take meridian exercise 20minute a day for 4 weeks. Subjective healthiness and satisfactoriness is investigated through a question for verification the effect of meridian exercise. Collected data is analized by SPSS program. major results are as follows. 1. Meridian Exercise for health is composed of rhythmic rubbing movement with music to the fourteen spots a the body suitable for acupuncture, including the inside and outsides of arms, tips of fingers, all sides of the leg, Use chest and abdominal area, shoulder, the head, and the face. 2. Among the people investigated, 50.0% were age twenty and 53.6% were 166cm and taller, both groups being the most abundant. 50% of the people were smokers, of which 64,3% smoked ten cigareties or less a day. 96.4% answered that they usually do not exercise. 3. Their subjective healthiness was significantly higher after performing meridian excercise, but life satisfactonness was not statistically significant. 4. Subjective healthiness and life satisfactoriness before and after performing meridian excercise did not have any significant relationships.

청소년의 여드름과 정서상태와의 관련성 연구

김광옥,차영애 한국미용학회 1999 한국미용학회지 Vol.5 No.2

Acne is a prevalent skin disease in adolescence, which may develop undesirable effects such as leaving scars due to misunderstanding and failure to control. Thus this study was conducted to find out the emotional impact of acne so that the result could provide the basic information for health education in schools, and for the treatment and control of acne. The data were collected from 1042 students at 3 middle schools and 3 high schools in Pusan by using a structured and self-administered questionnaire for a period from December 1 to December 20, 1998, and were analyzed for general characteristics, characteristics related to acne, the status of acne control and relationships between acne and emotional impact by using SPSS/PC+ program. The main results were as follows. 1. The relationship between the acne prevalence and the emotional status represented significant difference in the total categories of depression and in category related to worthlessness, and the acne prevalence, however, did not affect self-esteem. The score in the category of adaptation to school life related to the perception of school surroundings was significantly lower in the group with acne. 2. All categories related to emotional status such as depression, self-esteem adaptation to school life showed significant differences according to the severity of acne conditions, as showing the severe the acne condition, the more the depression and the lower the self-esteem and the adaptation to school life. 3. In the relationship between response to acne and emotional status, there were significant differences in categories related to self-esteem in school and school activities, the total categories in adaptation to school life. Especially, the effect of response to acne was significant in depression categories with significant differences in the categories of worthlessness helplessness and loneliness. 4. The relationship between the period of having acne and emotional status showed a significant difference in category related the adaptation to school life, especially, as the longer the period having acne, the lower the tendency for the adaptation to school life.

여성 생식호르몬 영향에 의한 자궁내막과 자궁내막암에서 텔로메레이즈 ( TELOMERASE ) 의 활성

서성일,이태성,차순도,조치흠,추영애 대한부인종양 콜포스코피학회 1999 Journal of Gynecologic Oncology Vol.10 No.1

During the reproductive period, human endometrium undergoes a pattern of cyclic change. Human endometrium undergoes a complex pattern of proliferation, secretory activity, and menstruation over an approximately 28 days period. Proliferative activity is highest during late proliferative phase under influence of estrogen, and minimal activity in the late secretory and menstrual phase. To study a possible change of telomerase activity during menstrual cycle, telomerase activities in normal and hormone treated endometrium were tested using telomerase repeat amplification protocol(TRAP) assay. Telomerase activities were detected in 9 of 10 proliferative endometrium(90%), and maximal activity was shown in late proliferative phase. Only 3 of 10 secretory endometrium(30%) revealed weak activity. However telomerase activity was not detected in menstrual phase endometrium(N 2) and senile endometrium(N=3). Four of tamoxifen treated endometrium(N 4) and 1 of provera treated endometrium(N 3) Levels of telomerase activity of treated endometrium(N 4) and late proliferative endometrium(N 6) were as high as them of detected in endometrial cancer and hyperplasia. Above findings reveal that telomerase activity of endometrium is changed according to menstrual cycle, And the level of telomerase activity is related to proliferative activity of endometrium that is dependent on the status of female sex steroid hormone and tamoxifen treatment. $quot;

자궁경부 상피세포암의 암화과정에 따른 인유두종 바이러스 감염과 Telomere 길이 및 Telomerase의 활성

서민호,이태성,박종하,차순도,조치흠,추영애,백원기,서성일 대한부인종양 콜포스코피학회 1997 Journal of Gynecologic Oncology Vol.8 No.1

E6 and E7 proteins produced by oncogenic HPV bind to the protein products of cellular tumor suppressor genes p53 and Rb, respectively. This mechanism has been suggested to contribute to the oncogenesis of HPV-infected carcinoma. The cells which are blocked the function of p53 and pRb protein continue to divide by bypassing M1 stage known as antiproliferative mechanism but telomeres, the genetic elements at the ends of chromosomes, continue to shorten until the telomeres are so short that further replication is prevented(M2 stage). But telomeres can be maintained if telomerase is derepressed, giving rise to a immortal cell. The present study has been investigated the presence of HPV, telomere length and telomerase activation in cervical carcinomas. HPV DNA were detected by polymerase chain reaction in 17 of 19 precancerous lesions and cervical carcinoma specimens; HPV16 was detected in 12 cases, HPV18 in one case, HPV33 in two cases, and HPV58 in two cases. Overall, the prevalence of HPV was 89.5%. To study the difference of telomere length in cervical carcinomas and each normal counterpart, DNAs were digested with Hinfill and Rsal to liberate the terminal restriction fragments(TRF). TRFs were resolved on agarose gels and detected by hybridization to the telomeric probe. This result indicated that there were no significant difference of TRF length in samples tested expept two cases. TRF length of one carcinoma specimen was found to be significantly increased as compared with normal counterpart, but the other was found to be significantly decreased. Telomerase activity was detected in 4 of dysplasia specimens(5 cases), all of carcinoma in situ(CIS), and 6 of 8 invasive carcinoma. Overall, telomerase activity was detected in 84%. The degree of telomerase activity was high in 2 of dysplasia, 3 of CIS, and 3 of invasive carcinoma. And then there was no apparent association between HPV types and levels of telomerase activity. However, telomerase activity was depressed in invasive carcinoma as compared to dysplasia and CIS. These results suggest that HPV may be a possible causative agent in cervical carcinoma. In addition, telomerase activation may be necessary for the immortalization of cells and the progression of malignancy in cervical carcinoma.