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ELISA에 의한 국내 재래종 산양의 정상상태에서의 혈중 cortisol 농도
조규완,허주형,이은석,강정부,Cho, Kyu-woan,Hur, Ju-hyeong,Lee, Eun-sug,Kang, Chung-boo 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4
This study was performed to examine the charges of serum cortisol concentrations on the normal conditions in Korean native goats by enzyme-linked immunosorbent assay(ELISA). The Korean native goats were 4 to 30 months old and weighted 6.0 to 28kg. The goats were allowed ad libitum access to water and food. To minimize the stress with handing and blood sampling, animals were adapted for 2 weeks before the experiment. After adaptation, intravenouse catheter was inserted left intact a jugular vein of 6 goats and blood samples were done 2~3 days later. Experimental animals were divided into 2 groups which was non-catheterized and catheterized group in order to examine the effect for changes of serum concentration and circadian rhythm of cortisol. The results were obtained as follows ; The sensitivity of serum cortisol concentration was $20pg/m{\ell}$. The intra-assay and inter-assay coefficient of variation were below 2.5% and 5%, respectively. Serum concentration of cortisol was more higher in noo-catheterized group$(32.84{\pm}16.78ng/m{\ell})$ than catheterized group$(23.20{\pm}10.29ng/m{\ell})$. The difference according to months old in serum concentration of cortisol was the more higber on 4~6 than 7~12 and lowest over 12 months old. A circadian rhythm in the serum concentration of cortisol in Korean native goats was not found significantly with 2 hours sampling intervals.
한우(Bos taurus coreanae)의 정맥관흔적(rudimentum of ductus venous)의 증례
김종섭,조규완,서명득,원청길,Kim, Chong-Sup,Cho, Kyu-Woan,Suh, Myung-Deuk,Won, Chung-Kil 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.4
The observations of the anatomical closure of the ductus venosus (DV) and vestige of DV were studied in 22 cattle, ranging from 210-day-old fetus to 3-years old Korean native cattle. Vinylite solution was injected into the hepatic, portal, umbilical veins and caudal vena cavae of 22 specimens for vinylite corrosion casts. The DV originated at the confluence of the umbilical and portal veins and emptied into the left hepatic vein or posterior vena cava. The DVs were persisted in a 210-day-old fetus, a 240-day-old fetus, and a 270-day-old fetus. Two newborns, two 2-year-old and two 3-year-old cattle had no rudimentum of DV (6 cases, 31.58%). However, vestiges of DV in varying sizes were observed in a 14-day-old, a 3O-day-old, two 180-day-old and nine adult cattle (13 cases, 68.42%). The lengths of vestiges of DV were about 4.97~99.66 mm. Therefore, the present study demonstrates that DV in cattle can be degenerated during the late period of a pregnancy.
고라니(Hydropotes inermis) 장골과 천골이 유합된 1례
김종섭,고필옥,조규완,조재현,원청길,Kim, Chong-Sup,Koh, Phil-Ok,Cho, Kyu-Woan,Cho, Jae-Hyeon,Won, Chung-Kil 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4
The sacrum fused with ilium in a chinese water deer (Hydropotes inermis) was observed. The sacrum consists of four sacral vertebrae which was fused each other. In the ventral part of the fused pelvis, on the left, there was a 20.19 mm gap at the dorsolateral part of the sacroiliac articular part which is in between the promontory and the cranial border of the first ventral sacral foramen. On the right, there was a 14.34 mm gap at the dorsal part of sacroiliac articulation. In the dorsal view of the fused pelvis, the wing of sacrum and the wing of ilium were fused tightly, so that the boundary of the two bones was not recognizable. The left and right second dorsal sacral foramina and the third dorsal sacral foramen on the left were small. The third dorsal and ventral sacral foramina on the right were not formed.
김종섭,강병일,고필옥,조규완,허찬권,원청길,Kim, Chong-Sup,Kang, Byoung-Il,Koh, Phil-Ok,Cho, Kyu-Woan,Huh, Chan-Kwen,Won, Chung-Kil 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1
The fused and deformed lumbar vertebrae (L) of Thoroughbred horses were observed macroscopically. Results revealed five L in two cases, and six L in three cases. In two cases, the last two lumbar vertebrae were fused with each other. One case of the fused lumbar vertebra was observed in a horse with five L and the other in that with six L. In one case, the facet of second last lumbar vertebra was articulated with its anterior lumbar vertebra. On the other hand, four cases had the caudal border on the lateral tip of the lumbar transverse process partially overlapped with the cranial border of the following lumbar transverse process. In these cases, cranial and caudal tubers were formed on the overlapped part of the lumbar transverse process.
아프리카코끼리(Loxodonta africana)의 흉추골유합과 요추골, 천골 및 장골이 유합된 1례
김종섭,원청길,조규현,조규완,박중석,노규진,Kim, Chong-sup,Won, Chung-kil,Cho, Gyu-hen,Cho, Kyu-woan,Park, Joong-suk,Rho, Gyu-jin 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
The vertebrae of female African elephant (Loxodonta africana) of twenty-eight years old were observed macroscopically. The result was summarized as follows; the vertebral formula of African elephant was $C_7$ $T_{21}$ $L_3$ $S_4$ $Cd_{21}$. The total length of the vertebral column removed intervertebreal disks was 353 cm. The length of each segment of vertebral column was 44 cm in cervical, 142 cm in thoracic, 21 cm in lumbar, 27 cm in sacral and 119 cm in caudal vertebrae. The 17th and 18th thoracic vertebrae (T) were partially fused each other in four parts: the transverse processes of 17th and 18th T, caudal articular process of 17th T and cranial articular process of 18th T, left mammilary process of 18th T and left transverse process of 17th T, and vertebral arch of 17th and 18th T, respectively. Others partial fusions also observed among the third luwbar, sacrum and ilium. These were in between transverse process of third lumbar vertebra and cranial parts of wing of sacrum, lateral part of sacrum and tuber sacrale, respectively. The sternum was consisted of three pieces; one is a part of anterior prestemum, two is the part from caudal demifacet at second facet to cranial demifacet at third facet in middle mesosternum, which is divided vertically into an half at second and third facets, respectively, and the last is the part between caudal demifacet of third facet in middle mesosternum and the posterior xiphisternum. There are 21 pairs of ribs, six sternal, ten asternal ribs and the last five being floating ribs.
ELISA에 의한 소의 혈청 progesterone 농도 측정
강정부,손민수,이은석,조규완,김철호,Kang, Chung-boo,Son, Min-soo,Lee, Eun-sug,Cho, Kyu-woan,Kim, Chur-ho 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.3
This study was carried out the determine the progesterone concentration for serum by enzyme-linked immunosorbent assay(ELISA) in bovine adult at estrous, pregnant, after patuation and male, female calves of 1 month old, respectively. The results obtained were summarized as follows ; 1. The assay has a sesitivity of $0.1ng/m{\ell}$. 2. Intra and inter-assay coefficient of variation were 4.5%, 6.1~9.4% when used for the determination of progesterone in samples of bovine serum. 3. The percentages of recovery for progesterone added were between 88.0 to 88.9%. 4. Progesterone concentration of adult bovine serum at estrus, pregnant and after 1 day of parturition were $0.37{\pm}0.16$, $7.1{\pm}1.0$, $0.13{\pm}0.02ng/m{\ell}$, respectively. 5. There was no differences in serum progesterone concentration of calves both male($0.16{\pm}0.03ng/m{\ell}$) and female($0.15{\pm}0.04ng/m{\ell}$) on 1 month old. From these results, progesterone determination by ELISA is very applicable to detect of early pregnancy diagnosis, factorial analysis of reproductive disorder, and also reproductive physiological functions such as veterinary therapeutic measures and monitoring of cyclicity.
한국재래산양 태아 및 신생아의 결장상피 발달에 관한 투과전자현미경적 연구
김종섭,정순희,원청길,이종환,조규현,곽수동,조규완,김무강,송치원,Kim, Chong-Sup,Jung, Soon-Hee,Won, Chung-Kil,Lee, Jong-Hwan,Cho, Gyu-Hyen,Kwak, Soo-Dong,Cho, Kyu-Woan,Kim, Moo-Kang,Song, Chi-Won 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
The morphological development of colonic epithelia in fetuses between 60-. 90-, and 120-days gestation and neonates of Korean native goat were investigated by transmission electron microscopy. In the 60-day-old fetuses, the colonic epithelial cells contained nuclei, nucleoli, mitochondria, free ribosomes, and shoo granular and agranular endoplasmic reticula. The zonula occludens, zonula adherens, desmosomes, short microvilli, and masses of glycogen granules were also obsrved. The goblet cells contained a few secretory granules. In the 90-day-old fetuses, the cell organelles of the colonic epithelial cells were better developed than those in the 60 day old fetuses. Increased number of endoplasmic reticula, digitiform intercellular junctions, mitochondria, and Golgi complexes was observed. The goblet cells contained a lot of secretory granules. In the 120-day-old fetuses, the colonic epithelial cells contained long microvilli and well developed cell organelles. The nuclear cleft and large intercellular space were also appeared. Nunerous fibroblasts were seen in the basement membrane. The number of goblet cells was further observed. In the 120 day old fetuses, all colonic epithelial cells shape simple columnar cells. In newborns, the colonic epithelial cells were covered with extensive microvilli. There were many goblet cells with a lot of secretory granules protruding into the intestinal lumen, and some goblet cells secreted their secretory granules into the lumen. In the 60-and 90-day-old fetuses, the colonic epithelial cells appeared to be either simple columnar or stratified columnar depending on areas.
조규완 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-
Feline lymphoma is the most common haemostatic neoplasm in cats and has higher prevalence in cats than in human and other domestic animals. Feline lymphoma review addresses some aspects of the epidemiology, clinical features, imminopenotyping, biologic behavior, genetics of viruses that cause a variety of hematopoietic disorders in cats, cytogenetic chromosome abnormalities and chemotherapy. Feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) status of cats are associated among lymphoma, leukemia and other diseases. The viruses described may be different pattern of geographical distribution, and the diseases manifestations may vary according to environmental and / or genetic characteristics and clinical feature of the cat. The pathogenic mechanisms used by these viruses are of most interest since they may recapitulate growth dysregulation steps also occurring in other hematopoietc malignancies. Feline lymphoma have become the focus of veterinarian and researcher and representing, since in most cases no specific antiviral treatment and chemotherapy for feline lymphoma, also need a further challenge for future basic research on characteristics and pathogenesis of feline lymphoma since little is known until recently.