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해양 천연물로부터 면역기능 조정제 렉틴 개발: MLA-I, MLA-II, MLA-III의 특성
정시련(See Ryun ChUng),김장환(Jang Hwan Kim),전경희(Kyung Hee Jeune) 대한약학회 1995 약학회지 Vol.39 No.3
Three new lectins, MLA-I, MLA-II and MLA-III, have been isolated and purified from the hemolymph of Meretrix lusoria and reported previously. Biophysicochemical characteristics were investigated with these three MLA lectins. The MLA lectins agglutinated human erythrocytes nonspecifically and proved as D-galactose group carbohydrate specific. Molecular weight of MLA-I, II and III were estimated to be 330, 500 and 31OKD, respectively, by gel filtration on Sepharose CL-6B column. On SDS-polyacrylamide gel electrophoresis, MLA I was dissociated into a single subunit of 42KD, MLA-II was into the twelve subunits of 46, 32, 30, 28, 25, 23, 22, 20, 19, 16, 15, and 14KD, and MLA-III was into the two subunits of 72 and 44KD. The pI of MLA-I, II, III were 4.0, 4.9 and 5.0. Amino acid analysis revealed a high contents of acidic and hydroxy amino acids, and a paucity of sulfur containing amino acids. Proline was not contained in MLA-II.
The Influence of Mineral Nutrients on Growth and Alkaloid Levels in Lycopersicum esculentum
정시련(See-Ryun CHUNG) 한국식품영양과학회 1972 한국식품영양과학회지 Vol.1 No.1
Twenty kinds of minerel nutrient solution were prepared and supplied to the tomatoes planted in Norwegian quartz pots.<br/> These plants were cultured for nine weeks and several physiogical phenomena were observed duirng the growing period, and after harvest, the alkaloid contents were determined.<br/> The highest growth potential was in NS 3 group while the highest alkaloid content wasein KMg 18 group.
정시련(See Ryun Chung),김장환(Jang Hwan Kim),소명숙(Myung Suk So),김무경(Moo Kyung Kim),현태금(Tae Geum Hyun),전경희(Kyung Hee Jeune) 大韓藥學會 1991 약학회지 Vol.35 No.5
Two kinds of new lectin fractions (LOA-I, LOA-II) were obtained from loach (Misgurnus spp.) meat by 0.15 M NaCl extraction, salt fractionation, ion exchange and hydroxyapatite column chromatographies. On polyacrylamide gel electrophoresis, LOA-I exhibited one major and a few minor bands, but LOA-II exhibited three minor bands. The partially purified loach lectins agglutinated not only erythrocytes of human B and AB type, rabbit, dog, but also murine splenic lymphocytes. Agglutinability was relatively labile at various pH and stable at increasing temperature, but was not affected by tested several metal ions. By the sugar specificity test, D-glucosamine and methyl-beta-galactopyranose inhibited agglutinating activity at a final concentration of 3 mM. The lectins contained relatively high amounts of aspartic acid, valine and leucine, but sulfur containing amino acids, cystein, methionine and isoleucine were not determined. LOA-I, LOA-II lectins were nonmitogenic toward murine lymphocytes.
정시련(See-Ryun Chung),전경희(Kyung-Hee Jeune Chung),김경애(Kyong-Ae Kim) 한국생약학회 1981 생약학회지 Vol.12 No.1
White kidney bean (Phaseolus vulgaris, C, W. K.B.) and Cercis chinensis(C.C) belonging to the Leguminosae contained strong hemagglutinating proteins. The crude lectin from W.K.B. were purified by DEAE Sephadex A-50 column chromatography. The 0.1M peak from this procedure demonstrated acceptable criteria, e.g., yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. Further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity were recovered in 0.1 M peak. I was electrophoresed and found to migrate as a single band. These suggests that the condition of purification for W.K.B. lectin was encourageable but these methods did not brought agreeable satisfaction to the Cercis chinensis lectin.
정시련(See Ryun Chung),정수민(Su Min Chung),이승호(Seung Ho Lee),전경희(Kyung Hee Jeune) 대한약학회 1996 약학회지 Vol.40 No.4
The erythrocytes agglutination test was applied to the common korean plants for lectin activity screening by using human blood. During the four years, 108 species from 46 families of floras were collected, identified and subjected to the test after being divided into several different parts. Only 13 species demonstrated strong lectin activities. Meanwhile 66 species did not shown any agglutination. All others were observed as having low activity or as having hemolytic constituents.
정시련(See Ryun Chung),전경희(Kyung Hee Jeune-Chung) 한국생약학회 1984 생약학회지 Vol.15 No.1
嶺南大學校 藥草園에서 수집한 15種의 콩과植 物種子를 대상으로 사람의 血液과 토끼 血液으로 檢索試驗한 結果 7種 以上의 各種 血液에 대해서 렉틴(lectin)活性을 나타내었다. 이중 5種을 선정하여 電氣泳動試驗結果 各各은 많은 band를 나타내어 이들은 많은 종류의 蛋白質로 되었음을 알 수 있었으며 蛋白質定量結果 多樣한 차이가 나타났다. 한편 이들 콩과植物 렉틴사이에 서로 構造物 相關性이 있는지를 알아보기 위해 免疫生化學的인 硏究를 遂行했든 바, 몇종의 경우는 PHA, E-PHA, L-PHA, MBLA 렉틴등과 免疫生化學的으로 類似한 것으로 밝혀졌으며 더욱 ion exchange chromatography로 分離精製한 PHA에는 면역학적인 실형결과 2종의 抗原이 存在함을 알 수 있었다. 以上의 實驗에서 演者등은 數種 콩과植物을 대상으로 이들의 렉틴含有與否와 PHA, MBLA 렉틴을 이용하여 얻은 antisera로 免疫化學的 實驗을 하여 PHA, MBLA렉틴과 기타 콩과 植物렉틴과의 蛋白質構造내지 機能上의 相關性등을 연구 검토하였다.
해양 패류 렉틴성분 연구(V) 반지락조개의 렉틴성분 분리정제에 관한 연구
정시련(See Ryun Chung),김장환(Jang Hwan Kim),전경희(Kyung Hee Jeune-Chung) 대한약학회 1987 약학회지 Vol.31 No.2
The result of the screening of lectins on 28 species of marine shell fishes (mollusks) showed that 10 species (Tapes philippinarum, Cyclosunetta menstrualis, Neptunea arthritica cumingii, Omphalius pfeifferi carpenteri, Chlorostoma argyrostoma turbinatum, Chiorostoma argyrostoma lischkei, Semisulcosira corea, Neptunea polycosta, Babylonica japonica, Noverita didyma) were present hemagglutinating properties to human A, B, and 0 group, and animal blood erythrocytes. A new lectin from Tapes philippinarum was isolated and partially characterized. The lectin was purified by (NH4)2SO4 precipitation and ion exchange chromatography on DE 53 column. Six fractions were obtained from DE 53 column by salt gradient elution but only 0.3M, and 0.4M NaCl fraction had strong lectin activity. On its 0.3M NaCl fraction, purity was identified by polyacrylamide gel electrophoresis. This lectin was inhibited by N-acetyl-D-galactosamine. It seems that two kinds of lectin react as antigen by immunochemical studies.