http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
인간 기도 상피세포에서 증가된 뮤신 생성에 대한 쿠마린과 인도메타신의 억제작용
이재우(Jae Woo Lee),김길동(Kil-Dong Kim),전병규(Byeong Kyou Jeon),이충재(Choong Jae Lee) 대한약학회 2010 약학회지 Vol.54 No.5
We examined whether indomethacin, noscapine, coumarin, uridine and betaine affect airway mucin production induced by EGF or TNF-α from NCI-H292 cells. Cells were pretreated with each agent for 30 min and then stimulated with EGF or TNF-α for 24 h. Of the five compounds, coumarin suppressed airway mucin production induced by EGF or TNF-α. However, indomethacin suppressed airway mucin production induced by EGF. This result suggests that coumarin and indomethacin can regulate the production of mucin induced by EGF, by directly acting on airway epithelial cells.
Quercetin에 의한 혈관이완효과에 대한 알코올의 추가적인 역할
진영배(Youngbae Jin),제현동(Hyun Dong Je) 대한약학회 2010 약학회지 Vol.54 No.5
The aim of present study was to investigate the possible influence and related mechanism of additional alcohol on the flavonoid- induced arterial relaxation. Agonist-induced vascular smooth muscle contractions involve the activation of thick or thin filament pathway. However, there are no reports addressing the question whether this pathway is involved in quercetin-induced relaxation cotreated with alcohol in rat aortae contracted with phorbol ester, fluoride or thromboxane A2 mimetic U-46619. We hypothesized that cotreated alcohol plays a role in vascular relaxation evoked by quercetin in rat aortae. Endothelium-denuded arterial rings from male Sprague-Dawley rats were used and isometric contractions were recorded using a computerized data acquisition system. Quercetin inhibited phorbol ester, fluoride or thromboxane A2-induced contraction regardless of endothelial function. However, alcohol didn't decrease any agonist-induced contraction. Interestingly, only in thromboxane A2-induced contraction, synergistic results were observed in aortae denuded and cotreated with quercetin and alcohol suggesting that additional pathways different from antioxidation or endothelial nitric oxide synthesis might be involved in the vasorelaxation. In conclusion, in the agonists-contracted rat aortae, quercetin and alcohol together showed synergistic response regardless of endothelial function in an agonist-dependent manner.
새로운 항궤양제(IY-80843과 IY-80845)의 기니픽 위점막세포 $H_2$-수용체에 대한 결합반응연구
김승희,우승희,정숙영,문애리,이송득,김동연,이승룡 대한약학회 1995 약학회지 Vol.39 No.2
Receptor binding study was carried out as an in vitro assay to test the anti-ulcer effect for newly synthesized test compounds(IY-80843 and IY-80845) which were reported to have a strong anti-secretory effect in Shay-ligated rats. The histamine H$_{2}$-receptor fraction was prepared from the membranes of the isolated gastric glands in guinea pigs and $^{3}$H-cimetidine was used as a radioligand. The binding of $^{3}$H-cimetidine to the membranes of the isolated gastric glands was found to be time dependent, saturable and confined to a single population of binding sites with $K_{D}$ value of 0.13$\pm$0.03 $\mu{M}$ and B$_{max}$ value of 52.5$\pm$1.5 pmol/mg. From the competition experiments, both IY-80843 and IY-80845 were shown to have a strong blocking effect against binding of $^{3}$H-cimetidine to the histamine H$_{2}$-receptor. The IC$_{50}$, Ki, and Hill coefficient(nH) values for IY-80843 were 0.18$\pm$0.02 $\mu{M}$, 0.16$\pm$0.02 $\mu{M}$, and 0.97$\pm$0.15, respectively and those values for IY-80845 were 0.27$\pm$0.02 $\mu{M}$, 0.24$\pm$0.02 $\mu{M}$, and 0.82$\pm$0.13, respectively. The results demonstrated that the blocking effects of IY-80843 and IY-80845 were 7 and 5 times stronger than that of cimetidine, respectively. Therefore, the newly synthesized compounds, IY-80843 and IY-80845, appeared to be the highly potent competitive inhibitors of histamine on the H$_{2}$-receptor.
Propionibacterium acnes에 대한 천연물의 항균효과 검색
최승만(Seung Man Choi),김민주(Min Joo Kim),최영호(Young Ho Choi),안호정(Ho Jung Ahn),윤여표(Yeo Pyo Yun) 大韓藥學會 1998 약학회지 Vol.42 No.1
Propionibacterium acnes is the pharmacological target site of antiacne drugs. We have examined the antiacne activity of ninety seven natural products which have been used as Korean traditional medicines in various skin disorders. The antibacterial activity of extracts from the natural products were evaluated against P. acnes ATCC 9616 by disc method. Twelve natural products showed the potent antibacterial activity against P. acnes, and were, selected for the minimal inhibitory concentration(MC) against P. acnes. MICs of nine extracts were below 0.3% (w/v) and Sophora flavescens showed the most potent activity with a MIC of less than 0.008%(w/v) against P. acnes. Thus, the results suggest that nine natural products including S. flavescens can be developed as sources of promising potent antiacne agents.
3,4,5-Trihydroxybenzoic Acid Methylester와 관련 화합물의 피부암 및 구강암 세포주에 대한 세포독성
이재숙(Jae Sug Lee),한두석(Du Seok Han),강정일(Jeong Il Kang),백종민(Jong Min Baek),백승화(Seung Hwa Baek) 대한약학회 2010 약학회지 Vol.54 No.2
The cytotoxic activity of 33,4,5-trihydroxybenzoic acid methylester and related compounds on the growth of normal cell lines, human skin melanoma cells and human oral epithelioid cell line were evaluated by the 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-[2-methoxy-4-nitro-5-sulfo-phenyl]-2-H-tetrazolium-5-caboxanilide (XTT) methods. 3,4,5-Trihydroxybenzoic acid methylester decreased the cell viability of human skin melanoma cells and human oral epithelioid cells shown by the MTT method and the cell adhesion activity of human skin melanoma cells and human oral epithelioid cells shown by the XTT method. In light microscopy, 100 μM 3,4,5-trihydroxybenzoic acid methylester showed the highest cytotoxic activity. These results suggest that 3,4,5-trihydroxybenzoic acid methylester has a potential anticancer activity.
배양한 대뇌피질세포에서 유발한 신경손상에 대한 콜린에스테라제 억제제의 영향
독고향(Hyang Dok Go),이광헌(Kwang Heun Lee),조정숙(Jung Sook Cho) 대한약학회 2002 약학회지 Vol.46 No.3
Alzheimer's disease (AD) involves neuronal degeneration with impaired cholinergic transmission, particularly in areas of the brain associated with learning and memory: Several cholinesterase inhibitors are widely prescribed to ameliorate the cognitive deficits in AD patients. In an attempt to examine if tacrine and donepezil, two well- known cholinesterase inhibitors, exhibit additional pharmacological actions in primary cultured rat cortical cells, we investigated the effects on neuronal injuries induced by glutamate or N-methyl-D-aspartate (NMDh), β-amyloid fragment (Aβ(25- 35)), and vatslous oxidative insults. Both tacrine and donepezil did not significantly inhibit the excitotoxic neuronal damage induced by g1utamate. However, taurine inhibited the toxicity induced by NMDh in a concentration-dependent fashion. In addition, taurine significantly inhibited the Aβ(25-35)-induced neuronal injury at the concentration of 50μM. In contrast, donepezil did not reduce the NMDA- nor Aβ(25-35))-induced neuronal injury, Tacrine and donepezil had no effects on oxidative neuronal injuries in cultures nor on lipid peroxidation in vitro. These results suggest that, in addition to its anticholinesterase activity the neuroprotective effects by taurine against the NMDA- and Aβ(25-35)-induced toxicity may be beneficial for the treatment of hn. In contrast, the potent and selective inhibition of central acetylcholinesterase appears to be the major action mechanism of donepezil.