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정선양(Sunyang Chung),황두희(Doohee Hwang),임종빈(Jongbin Yim) 한국기술혁신학회 2016 기술혁신학회지 Vol.19 No.4
본 연구는 경기도 남부지역의 대표적인 혁신클러스터인 판교테크노밸리를 연구대상으로 혁신클러스터의 성과 영향요인에 대한 실증분석을 통한 사례연구이다. 혁신클러스터의 평가를 위한 영향요인은 이론연구 및 문헌고찰을 통해 제도적・물리적・사회적 구성요인으로 구분하여 도출하였다(정선양, 2012). 특히 정선양(2012)에서 제안된 클러스터 구성요인은 혁신클러스터 기능 및 역할에 대한 연구분석의 틀은 기존의 이론과 연구를 통해 통합되어, 최근 많은 연구에 의해 실증분석의 틀로 제안되고 있다(예: 임종빈. 2013; 임종빈 외, 2016; 이상욱 외, 2014). 본 연구는 기 제시된 제도적 요인, 물리적 요인, 사회적 요인을 중심으로 판교테크노밸리의 혁신클러스터의 성과에 영향을 주는 요인에 대하여 실증분석하고, 혁신클러스터내 기업들의 기술협력이 이들 영향요인에 어떠한 영향을 미치는 가를 다중회귀분석을 활용하여 분석한다. 이 같은 실증분석은 기존에 제시된 혁신클러스터의 영향요인에 대한 변별력을 제고하고, 실제적인 혁신클러스터 공간에서의 운영과 성장, 향후발전에 영향을 미치는 구성요소들을 도출하는데 유용성을 줄 것이다. 무엇보다도 본 연구는 혁신클러스터 영향요인을 도출함에 따라 혁신클러스터의 혁신평가 실증분석을 통해 지표의 변별력을 향상해주고, 새로운 지표개발 및 평가적용에 합리성 제고 등을 기여할 것이다. 직접적으로 동 연구는 연구대상 및 사례로 하고 있는 판교테크노밸리의 정책적 시사점을 제공한다. This study is an empirical analysis that makes a survey on the evaluation of innovation cluster. For this purpose, we explore a case of innovation cluster, the Pangyo Techno Valley. This survey applies a research frame and evaluation contents that the innovation performance which is the institutional, physical, and social configurations of innovation clusters. In particular, this empirical analysis identifies the configuration factors which are the rational influence factors of individual subjects, in order to foster innovation clusters from the case study of the Pangyo Techno Valley. We can suggest that innovation policy from the results of innovation performance by applied configuration factors for evaluation of innovation cluster in order to enhance and facilitate for innovation cluster. Also, this study can provide lessons for regional policy implementation and new cluster policy agenda in Korea.
A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
정선도,최지혜,홍창완,이현지,박윤경,신정훈,박재원,박세호 대한면역학회 2009 Immune Network Vol.9 No.6
In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity. In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.
정선도,박윤경,신정훈,Hyunji Lee,Soo-Young Kim,이갑열,박세호 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.8
TGF-β-induced tolerogenic-antigen presenting cells (Tol-APCs) could induce suppression of autoimmune diseases such as collagen-induced arthritis (CIA) and allergic asthma. In contrast, many studies have shown that NKT cells are involved in the pathogenesis of Th1-mediated autoimmune joint inflammation and Th2-mediated allergic pulmonary inflammation. In this study, we investigated the effect of CD1d-restricted NKT cells in the Tol-APCs-mediated suppression of autoimmune disease using a murine CIA model. When CIA-induced mice were treated with Tol-APCs obtained from CD1d+/- or CD1d-/- mice, unlike CD1d+/-APCs, CD1d-/- Tol-APCs failed to suppress CIA. More specifically, CD1d-/- Tol-APCs failed to suppress the production of inflammatory cytokines and the induction of Th2 responses by antigen-specific CD4 T cells both in vitro and in vivo. Our results demonstrate that the presence of CD1d-restricted NKT cells is critical for the induction of Tol-APCs-mediated suppression of CIA.