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난자 세포질내 정자 주입술 후 동결보존 배아이식: 고식적 체외수정시술과의 비교 연구
김석현,지병철,정병준,김희선,류범용,방명걸,오선경,손철,서창석,최영민,김정구,문신용,이진용,Kim, S.H.,Jee, B.C.,Jung, B.J.,Kim, H.S.,Ryu, B.Y.,Pang, M.G.,Oh, S.K.,Shon, C.,Suh, C.S.,Choi, Y.M.,Kim, J.G.,Moon, S.Y.,Lee, J.Y. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3
The objective of this study was to compare retrospectively the survival and pregnancy rates(PR) of cryopresered-thawed embryos obtained from intracytoplasmic sperm injection (ICSI) or conventional in vitro fertilization (IVF). Ninety-six cycles of cryopresered-thawed embryo transfer (ET) were performed in 79 patients from June, 1996 to September, 1997 and grouped as followings: 20 cycles (16 patients) inseminated by ICSI (ICSI Group) and 76 cycles (63 patients) by conventional IVF (IVF Group). Slow-freezing and rapid-thawing protocol was used with 1.5M propanediol (PROH) and 0.1M sucrose as cryoprotectant. All embryos were frozen-thawed at the two pronuclear (2 PN) stage excluding four cycles in which the early cleavage stage embryos were frozen, and allowed to cleave in vitro for one day before ET. The duration from freezing to thawing was comparable in both groups ($mean{\pm}SD$, $112.1{\pm}80.0$ vs. $124.8{\pm}140.1$ days). The age of female ($31.2{\pm}3.4$ vs. $32.6{\pm}3.3$ years) and the endometrial thickness prior to progesterone injection ($9.4{\pm}2.0$ vs. $9.3{\pm}1.8$ mm) were also comparable in both groups. There was no significant difference in the outcomes of cryopreserved-thawed ET between two groups: survival rate ($85.2{\pm}16.1%$ vs. $82.2{\pm}19.7%$), cleavage rate ($96.9{\pm}6.7%$ vs. $94.7{\pm}13.0%$), cumulative embryo score (CES, $54.5{\pm}31.1$ vs. $49.0{\pm}20.0$), preclinical loss rate (5.0% vs. 5.3%), clinical miscarriage rate (0% vs 29.4%), clinical PR per transfer (35.0% vs. 22.4%), implantation rate (9.9% vs. 5.6%), and multifetal PR (42.9% vs. 17.6%). In conclusion, human embryos resulting from ICSI can be cryopreserved-thawed and transferred successfully, and the survival rate and PR are comparable to conventional IVF.
원심분무법에 의한 Al-Pb-Sn 계 합금 분말의 Si 첨가에 따른 분말특성 연구
이용호,조성석,이영근,정병준 대한금속재료학회(대한금속학회) 1991 대한금속·재료학회지 Vol.29 No.8
By using the centrifugal atomization of rapid solidification process, the alloy powder which contained Sn and Si on the basis of monotectic Al-Pb system were fabricated. Morphology, size distribution, particle diameter, hardness were examined. To investigate distribution pattern of each element in the alloy powder, X-ray dot mapping of EPMA was used. And so, to know the solubility of Si by RSP, X-ray diffraction experiment was acted. The shape of the obtained powder particles were not globe-shaped but generally ligament, direct drop and irregular-shaped. The size distribution showed bimodal distribution which have two peak at 50㎛ and 200㎛. The microstructure for Si addition showed dendritic structure. From the solidification structure, the cooling rate of the alloy powder was calculated to be 10²∼10^5 K/S. Within the experimental conditions, it was found that the Addition elements were and thoroughly dispersed in the matrix. In the present work, the solubility of Si in a phase was about 4wt% by rapid solidification.