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Curcumin-induced Cell Death of Human Lung Cancer Cells
이화신,박보배,유선녕,전호연,김부경,김애리,손동현,김예린,이상율,김동섭,안순철 한국생명과학회 2023 생명과학회지 Vol.33 No.9
Lung cancer is a type of cancer that has the highest mortality rate. It is mainly classified into small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Chemotherapy is used to treat lung cancer, but long-term treatment causes side effects and drug resistances. Curcumin is a bright yellow polyphenol extracted from the root of turmeric. It has biological activities, such as anti-oxidant, anti-cancer, and anti-inflammatory effects. In this study, we observed differential cell death in human lung cancer cells. Based on the results, curcumin at 10, 30, and 50 μM exhibited a dose-dependent inhibition on the cell survival of several lung cancer cells, with minor differential phenotypes. In addition, apoptosis, autophagy, and reactive oxygen species (ROS) regeneration were observed through flow cytometry. Curcumin dose-dependently increased these phenotypes in A549 (NSCLC) and DMS53 (SCLC), which were restored by corresponding inhibitors. Western blotting was performed to measure the level of expression of apoptosis- and autophagy-related proteins. The results indicate that Bax, PARP, pro-caspase-3, and Bcl-2 were dose-dependently regulated by curcumin, with seemingly higher Bax/Bcl-2 ratios in DMS53. In addition, autophagic proteins, p-AKT, p62, and LC3B, were dose-dependently regulated by curcumin. ROS inhibition by diphenyleneiodonium reduced the induction of apoptosis and autophagy generated by curcumin. Taken together, it is suggested that curcumin induces apoptosis and autophagy via ROS generation, leading to cell death, with minor differences between human lung cancer cells.
노재승(Jae-Seung Roh),안재상(Jai-Sang Ahn),김범준(Beom-Jun Kim),전호연(Ho-Yeon Jeon),서승국(Seung-Kuk Seo),김석환(Suk Hwan Kim),이상우(Sang-Woo Lee) 한국표면공학회 2009 한국표면공학회지 Vol.42 No.2
This study is the research on the thermal emissivity depending on the bulk graphite’s alignment degree. Bulk graphites were manufactured by uni-axial pressing and subsequent heat treatment of natural graphite flakes with organic binder. The samples were prepared to be 0° (relative to the 002 c-face), 45°, and 90° (relative to the 100 a-face) for measuring alignment degree. The alignment degree of the sample was measured by XRD. The thermal emissivity was measured by infrared thermal image camera at 100oC and compared with the value obtained by Infrared spectroscopy. The alignment degree and thermal emissivity of 0° sample were measured to be 0 and 0.70 respectively. And those of 90° sample were 0.73 and 0.80 respectively. The emissivity value was correlated with obtained by IR spectroscopy. Therefore it was considered that the thermal emissivity of the bulk graphite is correlated with the alignment degree.