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      • SCOPUSKCI등재

        Variation of Nitrate Concentrations and δ<sup>15</sup>N Values of Seawater in the Drake Passage, Antarctic Ocean

        장양희,김부근,신형철,홍창수,Jang, Yang-Hee,Khim, Boo-Keun,Shin, Hyoung-Chul,Sigman, Daniel M.,Wang, Yi,Hong, Chang-Su Korea Institute of Ocean Science Technology 2008 Ocean and Polar Research Vol.30 No.4

        Seawater samples were collected at discrete depths from five stations across the polar front in the Drake Passage (Antarctic Ocean) by the $20^{th}$ Korea Antarctic Research Program in December, 2006. Nitrate concentrations of seawater increase with depth within the photic zone above the depth of Upper Circumpolar Deep Water (UCDW). In contrast, ${\delta}^{15}N$ values of seawater nitrate decrease with depth, showing a mirror image to the nitrate variation. Such a distinct vertical variation is mainly attributed to the degree of nitrate assimilation by phytoplankton as well as organic matter degradation of sinking particles within the surface layer. The preferential $^{14}{NO_3}^-$ assimilation by the phytoplankton causes $^{15}{NO_3}^-$ concentration to become high in a closedsystem surface-water environment during the primary production, whereas more $^{14}{NO_3}^-$ is added to the seawater during the degradation of sinking organic particles. The water-mass mixing seems to play an important role in the alteration of ${\delta}^{15}N$ values in the deep layer below the UCDW. Across the polar front, nitrate concentrations of surface seawater decrease and corresponding ${\delta}^{15}N$ values increase northward, which is likely due to the degree of nitrate utilization during the primary production. Based on the Rayleigh model, the calculated ${\varepsilon}$ (isotope effect of nitrate uptake) values between 4.0%o and 5.8%o were validated by the previously reported data, although the preformed ${\delta}^{15}{{NO_3}^-}_{initial}$ value of UCDW is important in the calculation of ${\varepsilon}$ values.

      • KCI등재

        Mung Bean Coat Ameliorates Hyperglycemia and the Antioxidant Status in Type 2 Diabetic db/db Mice

        장양희,강민정,최은옥,신말식,김정인 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1

        The hypoglycemic and antioxidant effect ofmung bean coat (MBC) in an animal model of type 2diabetes was investigated. A 95% ethanol extract of MBCinhibited yeast α-glucosidase activity by 48.6% in vitro,which was 1.4× stronger than inhibition by acarbose at aconcentration of 0.5 mg/mL. Five week old db/db micewere fed an AIN-93G diet or a diet containing the MBCextract at 1% for 7 weeks. Blood glycated hemoglobin andserum glucose levels, as well as the homeostasis modelassessment for insulin resistance in MBC group mice, weresignificantly (p<0.01) lower than in control group mice. Consumption of the MBC extract reduced thiobarbituricacid reactive substance levels and elevated activities ofsuperoxide dismutase, catalase, and glutathione peroxidasein the liver, compared with the control group. MBC can beeffective for improving hyperglycemia and the antioxidantstatus in type 2 diabetes mellitus.

      • KCI등재

        남극해 드레이크해협 해수의 질산염 농도와 질소동위원소 값의 변화

        장양희,김부근,신형철,Daniel M. Sigman,Yi Wang,홍창수 한국해양과학기술원 2008 Ocean and Polar Research Vol.30 No.4

        Seawater samples were collected at discrete depths from five stations across the polar front in the Drake Passage (Antarctic Ocean) by the 20th Korea Antarctic Research Program in December, 2006. Nitrate concentrations of seawater increase with depth within the photic zone above the depth of Upper Circumpolar Deep Water (UCDW). In contrast, δ 15N values of seawater nitrate decrease with depth, showing a mirror image to the nitrate variation. Such a distinct vertical variation is mainly attributed to the degree of nitrate assimilation by phytoplankton as well as organic matter degradation of sinking particles within the surface layer. The preferential 14NO3- assimilation by the phytoplankton causes 15NO3− concentration to become high in a closedsystem surface-water environment during the primary production, whereas more 14NO3− is added to the seawater during the degradation of sinking organic particles. The water-mass mixing seems to play an important role in the alteration of δ 15N values in the deep layer below the UCDW. Across the polar front, nitrate concentrations of surface seawater decrease and corresponding δ 15N values increase northward, which is likely due to the degree of nitrate utilization during the primary production. Based on the Rayleigh model, the calculated ε (isotope effect of nitrate uptake) values between 4.0‰ and 5.8‰ were validated by the previously reported data, although the preformed δ 15NO3− initial value of UCDW is important in the calculation of ε values. Seawater samples were collected at discrete depths from five stations across the polar front in the Drake Passage (Antarctic Ocean) by the 20th Korea Antarctic Research Program in December, 2006. Nitrate concentrations of seawater increase with depth within the photic zone above the depth of Upper Circumpolar Deep Water (UCDW). In contrast, δ 15N values of seawater nitrate decrease with depth, showing a mirror image to the nitrate variation. Such a distinct vertical variation is mainly attributed to the degree of nitrate assimilation by phytoplankton as well as organic matter degradation of sinking particles within the surface layer. The preferential 14NO3- assimilation by the phytoplankton causes 15NO3− concentration to become high in a closedsystem surface-water environment during the primary production, whereas more 14NO3− is added to the seawater during the degradation of sinking organic particles. The water-mass mixing seems to play an important role in the alteration of δ 15N values in the deep layer below the UCDW. Across the polar front, nitrate concentrations of surface seawater decrease and corresponding δ 15N values increase northward, which is likely due to the degree of nitrate utilization during the primary production. Based on the Rayleigh model, the calculated ε (isotope effect of nitrate uptake) values between 4.0‰ and 5.8‰ were validated by the previously reported data, although the preformed δ 15NO3− initial value of UCDW is important in the calculation of ε values.

      • Effect of Paraquat on Photosynthetic Pigment, Soluble Protin and Reducing Sugar Contents in Spinach C

        Chang Yang Hee,Park Won Hyuck 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

        제초제 paraquat(PQ)가 시금치의 분리된 엽록체에 미치는 영향을 규명하기 위하여 50ppm 농도의 paraquat를 처리한 후, 각각 명·암하에서 3, 6, 9, 12, 24시간 후의 광합성 색소, 단백질 및 환원당 함량을 조사하였다. 24시간후 ligh+PQ처리군과 dark+PQ처리군에서 총엽록소 함량은 light 단독처리군과 dark 단폭처리군에 비해 15% 및 14%까지 각각 감소하였다. Carotenoid 함량의 경우 ligh+PQ처리군과 dark+PQ처리군에서 24시간후 light 단독처리군과 dark 단독처리군에 비하여 44% 및 16%가 각각 감소하였다. 그러므로 엽록체내 광합성색소 함량은 시간의 경과에 따라 점차 감소하며 광합성 색소에 대한 PQ의 영향은 명조건하에서 더욱 효과적임을 알 수 있다. 단백질 함량은 light+PQ 처리군에서 급격한 감소를 보였으나, 다른 처리근에서는 거의 변화가 일어나지 않았다. 24시간 후 light+PQ 처리군에서는 light 단독처리군에 비해 단백질 함량이 45% 감소하였다. 환원당 함량은 3시간 경과후 가장 큰 감소를 나타내었으며, light 단독처리군과 light+PQ 처리군에서는 초기값에 비해 38% 및 44% 로 각각 감소하였다. 그러나 3시간 이후에는 거의 변화가 나타나지 않았다. 이상의 결과에서 PQ 처리에 의하여 시금치 엽록체내의 광합성색소, 단백질 및 환원당 함량의 감소가 관찰되며 이와 같은 현상은 light+PQ처리군에서 더욱 현저하였다. 따라서 light+PQ 처리시 식물 대사과정 경로에 저해를 일으켜 식물의 여러 생리 작용에 큰 영향을 미치는 것으로 사료된다. This study was undertaken to investigate the effects of paraquat on photosynthetic pigment, soluble protein and reducing sugar contents in spinach chloroplasts under light and dark. The results obtained were as follows; 1. Total chlorophyll contents in light+PQ-treated group were decreased to 15% as compared with light-treated group, and those in light+PQ-treated group to 14% as compared with dark - treated group at 24hr. In light +PQ- treated groups carotenoid content with PQ was reduced to 44% as compared with group without PQ at 24hr. In dark-trested groups, the amount of carotenoid with PQ was reduced to 16% as compared with group without PQ at 24hr. 2. In light- treated groups, addition of paraquat to chloroplasts caused a rapid reduction of soluble protein content to 45% as compared with group without PQ at 24hr. 3. The percentages of decrease in reducing sugar content under illumination were 44% (with PQ) and 38% (without PQ) of the initial value. It is considered that paraquat triggers the destruction of existing pigment, the inhibition of pigment biosynthesis, the block of protein biosynthesis and the reduction of reducing sugar so that paraquat has inhibitory effects on the pathway of plant metabolism.

      • KCI등재

        Antioxidant Effect of Phaeodactylum tricornutum in Mice Fed High-fat Diet

        강민정,김상무,정수미,최하늘,장양희,김정인 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1

        High-fat diet (HFD)-induced obesity is associated with oxidative stress. The purpose of this study was to examine the antioxidant effect of Phaeodactylum tricornutum extract in mice with diet-induced obesity. Four-week-old C57BL/6J mice were fed a normal diet or HFD with and without 0.7% P. tricornutum lipid extract corresponding to 0.2% fucoxanthin for 8 weeks. P. tricornutum significantly decreased body weight and epidydimal white adipose tissue in mice fed the HFD. Serum triglyceride, glucose,insulin, and leptin levels, as well as homeostasis model assessment for insulin resistance (HOMA-IR) values, were significantly lower in the P. tricornutum group than in the HFD group. P. tricornutum significantly decreased thiobarbituric acid reactive substances (TBARS) and increased glutathione and the activities of superoxide dismutase,catalase, and glutathione peroxidase in the liver compared with the HFD group. Thus, P. tricornutum could exert antiobesity and antioxidant effects in mice fed a HFD.

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