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허용,이상목,이섭,유환국,안욱수,김병열,이정호,유회성,Hur, Y.,Lee, S.M.,Lee, S.,Yu, H.K.,Ahn, W.S.,Kim, B.Y.,Lee, J.H.,Yu, H.S 대한흉부심장혈관외과학회 1991 Journal of Chest Surgery (J Chest Surg) Vol.24 No.10
Congenital cysts of the mediastinum are rare, but interesting lesions. Such cysts are important because they may produce distressing symptoms and because some have a malignant potential. Mediastinal cysts of foregut origin represent an important diagnostic group. Classified according to their anomalous embryonic origins they include pericardial, bronchogenic, esophageal, enteric, and nonspecific cysts. A series of 11 consecutive surgically treated cases from the Dep. of Thoracic & Cardiovascular Surgery in National Medical Center from Oct. 1959 to Dec. 1990, all successfully resolved without mortality. The 8 bronchogenic, 1 enteric, & 2 non specific cysts are included in this series. And 2 of 11 were asymptomatic patients presented. The clinical & pathological behavior of the individual cysts comprise a wide variety of patterns. Proper utilization of the many diagnostic tests available can accurately characterize the cysts. The exact histologic diagnosis, however, is rarely made prior to operation.
허용,유환국,안욱수,김병열,이정호,유회성,Hur, Y.,Yu, H.K.,Ahn, W.S.,Kim, B.Y.,Lee, J.H.,Yu, H.S. 대한흉부심장혈관외과학회 1990 Journal of Chest Surgery (J Chest Surg) Vol.23 No.1
A total of 129 patients with a confirmed diagnosis of primary lung cancer were treated at Dep. of Thoracic k Cardiovascular Surgery, National Medical Center, Seoul, Korea, between July, 1981 and Dec., 1988. Particular emphasis was given in this review to the 72 patients that underwent surgical resection of their primary lung lesion. Factors such as histology, type of resection, sex, age, staging, and degree of dissemination were considered possible influences on survival. The age group of fifty k sixty decade occupied 55.8 %, and the youngest being 24 years and oldest 78 years. The incidence ratio of male to female was 3,2:1. The subjective symptoms of the patients were coughing [72.6%], chest pain [48.2%] and hemoptysis [35.6%], which were due to primary local influence. The confirmed diagnostic procedures were bronchoscopic biopsy, sputum cytology needle aspiration biopsy, open lung biopsy, anterior mediastinotomy & lymph node biopsy. By pathologic classifications, the squamous cell carcinoma was the most prevalent, 67 cases [51.9 %], and the adenocarcinoma in 36 cases [27.9%], undifferentiated small cell carcinoma in 13 cases [10.1 %], undifferentiated large cell carcinoma in 9 cases [6.9%], bronchioloalveolar carcinoma was 4 cases [3.1%]. The lymph node dissection with pneumonectomy [42 cases], lobectomy [14 cases] and pneumonectomy [6 cases], lobectomy [9 cases] without lymph node dissection were performed. The post operative TNM Staging[AJC] in 72 cases were Stage I in 24 cases, Stage II in 27 cases, and Stage III in 21 cases. Overall resectable was possible in 72 cases [55.8 %], and the operation mortality was 5.6 % [4 cases].
An Efficient Method for Production of Extracellular Human Tissue Factor in Escherichia coli
Hwan Goo Yoo(유환구),Yang Jin Park(박양진),Woo-Yiel Lee(이우일) 한국생명과학회 2009 생명과학회지 Vol.19 No.5
인간조직인자는 혈액응고인자 factor VII 과 복합체를 형성하며 연속적인 혈액응고 연쇄반응을 촉매하는 효소 활성체이다. 복합체 형성에 필수적인 이 조직인자의 세포 외 부분이, 기존의 융합 단백질 및 히스티딘 말단이 없는 새로운 발현 벡터에 의해 대장균 내에서 과량 발현 되었다. 봉입체 형태로 발현된 재조합 인간조직인자는 DEAE-Sephacel 크로마토그라피 기술을 적용하여 분리, 정제 및 구조적 복원이 동시에 시도 되었다. 정제된 재조합 단백질은 SDS-PAGE 분석에서 순수한 형태로 나타났으며, 생물학적 활성도 또한 기존의 조직인자와 거의 동등함을 보였다. 본 연구의 발현 및 정제 시스템은 이전의 보고에서 보여진 방법들에 비해 단백질 분해효소를 사용하지 않아 추가적인 크로마토그라피 과정이 필요 없어 좀 더 효율적이기 때문에 기존의 발현 시스템에 대해 대체할 수 있는 매우 유용한 방법으로 제공된다. Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a His? at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.
Aureobasidium Pullulans 의 Melanin색소 억제에 대한 연구
전희수(Hee Soo Yun),윤지예(Ji-ye Yun),임재현(Jae hyun Lim),유환구(Whan Koo You),이우일(Woo-Yiel Lee) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.2014 No.2
플루란(pullulan)은 Aureobasidium Pullulans 으로부터 생산되는 Carbohydrate의 일종으로 glucose 분자들이 각각 α-1,4 및 α-1,6 glucosidic bond로 결합된 maltotriose를 구성성분으로 이루어진 다당류 폴리머이다. 플루란은 1938년 발견된 이래로 Aureobasidium Pullulans으로부터 직접적으로 대량생산하여 화장품뿐만 아니라 의약품의 피복물질, 식품 첨가물 바이오 플라스틱 및 포장재 등 다양한 분야의 산업계에서 주목을 받고 있는 실정이다. 본 연구에서는 charcoal을 이용한 Aureobasidium Pullulans으로부터 플루란을 생산하는 기존의 방식을 개선하기 위하여 Vitmin C 및 꽈리(Ground cherry) 추출물을 이용한 Aureobasidium Pullulans의 Melanin색소 억제를 시도 하였다. Melanin 색소가 억제된 균류로부터 플루란의 생산은 공정의 효율성과 생산 단가 절감의 차원에서 주목된다.
Ti-6Al-4V 합금의 고온 성형시 미세조직 예측에 관한 연구
이유환,신태진,박노광,심인옥,황상무,이종수,Lee You Hwan,Shin Tae Jin,Park No Kwang,Shim In Ok,Hwang Sang Moo,Lee Chong Soo 한국군사과학기술학회 2004 한국군사과학기술학회지 Vol.7 No.4
A study has been made to investigate the high temperature deformation behavior of Ti-6Al-4V alloyand to predict the final microstructure under given forming conditions. Equiaxed and $Widmanst\ddot{a}tten$ microstructures of Ti-6Al-4V alloys were prepared as initial microstructures. By performing the compression tests at high temperatures$(700\~1100^{\circ}C)$ and at a wide range of strain rates$(10^{-4}\~10^2/s)$, various parameters such as strain rate sensitivity(m) and activation energy(Q) were calculated and used to establish constitutive equations. When the specimens were deformed up to strain 0.6, equiaxed microstructure did not show any significant changes in microstructure, while $Widmanst\ddot{a}tten$ microstructure revealed considerable flow softening, which was attributed to the globularization of a platelet at the temperature range of $800\~970^{\circ}C$ and at the strain rate range of $10^{-4}\~10^{-2}/s$. To predict the final microstructure after forming, finite element analysis was performed considering the microstructural evolution during the deformation. The grain size and the volume fraction of second phase of deformed body were predicted and compared with the experimental results.