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Lipomyces starkeyi KSM 22 Glucanhydrolase 용액의 치은 섬유아세포에 대한 영향
윤현정,정현주,김옥수,김도만,Yun, Hyun-Jeong,Chung, Hyun-Ju,Kim, Ok-Su,Kim, Do-Man 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.3
A novel glucanhydrolase from a mutant of Lipomyces starkeyi KSM 22 has additional amylase activity besides mutanolytic activity and has been suggested as promising anti-plaque agent. It has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 glucanhydrolase are desirable for its application as a dental plaque control agent. In human experimental gingivitis model and 6 month clinical trial, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effect was negligible. This study was aimed to evaluate the cytotoxic effect of Lipomyces starkeyi KSM 22 glucanhydrolase on human gingival fibroblasts. Primary culture of human gingival fibroblasts at the 4th to 6th passages were used. Glucanhydrolase solution was made from lyophilized glucanhydrolase powder from a mutant of Lipomyces stakeyi KSM 22 solved in PBS and added to DMEM medium to the final concentration of 0.5, 1, and 2 unit. Cells were exposed to glucanhydrolase solution or 0.1 % chlorhexidine and the cells cultured in DMEM with 10% FBS and 1% antibiotics as control. After exposure, the morphological change, cell attachment, and cell activity by MTT assay were evaluated in 0.5, 1.5, 3, 6, 24 hours after treatment. The cell proliferation and cell activity was also evaluated at 2 and 7 days after 1 minute exposure, twice a day. The cell morphology was similar between the Lipomyces smkeyi KSM 22 glucanhydrolase groups and control group during the incubation periods, while most fibroblasts remained as round cell regardless of incubation time in the chlorhexidine group. The numbers of the attached cells in the glucanhydrolase groups were comparable to that of control and significantly higher than the chlorhexidine group. The numbers of the proliferated cells in the glucanhydrolase groups at 7 days of incubation were comparable to the control group and higher than the chlorhexidine group. The cell activity in glucanhydrolase groups paralleled with the increased cell number by attachment and proliferation. According to these results, Lipomyces starkeyj KSM 22 glucanhydrolase has little harmful effect on attachment and proliferation of human gingival fibroblasts, in contrast to 0.1% chlorhexidine which was cytotoxic to human gingival fibroblasts. Therefore this glucanhydrolase preparation is considered as a safe and promising agent for new mouthwash formula in the near future.
용담사간탕(龍膽瀉肝湯)에 의해 유도된 MAP kinases 활성화를 통한 간암 세포주 HepG2의 세포사멸
윤현정,김한성,허숙경,황성구,박원환,박선동,Yun, Hyun-Jeong,Kim, Han-Seong,Heo, Sook-Kyoung,Hwang, Seong-Goo,Park, Won-Hwan,Park, Sun-Dong 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
The purpose of this study was to investigate the effect of Yong-dam-sa-gan-tang (YST) on apoptosis in HepG2 cells, First of all. to study the cytotoxic effect of methanol extract of YST on HepG2 cells, the cells were treated with various concentrations of YST and then cell viability was determined by XTT reduction method and trypan blue exclusion assay. YST reduced proliferation of HepG2 cells in a dose-dependent manner. To confirm the induction of apoptosis, HepG2 cells were treated with various concentrations of YST. The cleavage of poly AD P-ribose polymerase (P ARP), a substrate for caspase-3 and a typical sign of apoptosis, and the activation of caspase-3, procaspase-8 and procaspase-8 were examined by western blot analysis. YST decreased procaspase-3, procaspase-8 and procaspase-9 levels in a dose-dependent manner and induced the clevage of PARP. YST triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome c from mitochondria to cytosol. Furthermore, YST also downregulated the anti-apoptotic Bcl-2 and upregulated the pro-apoptotic-Bax. Therefore, this result suggest that YST induced HepG2 cell death through the mitochondrial pathway. Sustained activation of the Ras/Raf/MEK/ERK cascade in cells results in a cell cycle arrest and has been implicated in the differentiation of certain cell types, in many cases acting to promote differentiation. YST decreased the activation of Ras/Raf/MEK/ERK cascade in a dose-dependent manner. These results suggest that YST is potentially useful as a chemo-therapeutic agent in HepG2.
사람 대동맥 평활근 세포에서 이묘산(二妙散)의 항동맥경화 활성
윤현정 ( Hyun Jeong Yun ),허숙경 ( Sook Kyoung Heo ),이효승 ( Hyo Seung Yi ),김태훈 ( Tae Hoon Kim ),김동완 ( Dong Wan Kim ),김선모 ( Sun Mo Kim ),박선동 ( Sun Dong Park ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4
Objectives: This study was evaluated to elucidate the inhibitory potential of Imyosan (IMS) and its components, Phellodendri Cortex (PC; Phellodendron amurense Rupr., Hwangbaek in Korean) and Atractylodis Rhizoma (AR; Atratylodes lancea D.C., Changchool in Korean), on human aortic smooth muscle cells (HASMC) migration and production of matrix metalloproteinase (MMP)-2 and MMP-9 by TNF-α treatment. Methods: Cytotoxic activity of IMS and its components on HASMC was using 5-(3-caroboxy meth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Effect of IMS, PC and AR on TNF-α-induced HASMC migration underside of matrigel filter was stained with hematoxylin-eosin. And total number of cells that migrated to the underside of the filter was counted. MMP-2 and MMP-9 activity was evaluated by gelatin zymography assay. Results: The matrigel migration assay showed that IMS effectively inhibited the TNF-α-induced migration of HASMC. Moreover, IMS significantly inhibited MMP-9 activity. Our present study demonstrates that IMS and its components inhibit TNF-α-induced HASMC migration and MMP-9 activity. The inhibitory effect of IMS extract is more potent than that of its component herb extracts. Conclusions: These results provide evidence that IMS has multiple effects in the inhibition of HASMC migration and may offer a therapeutic approach to block HASMC migration.
윤현정(Hyun Jeong Yun),신나리(Nary Shin) 한국보육지원학회 2019 한국보육지원학회지 Vol.15 No.3
Objective: This study aims to develop and validate the musicality level of an individual child, based on the on performance tasks rubrics. Methods: The survey was conducted on 284 children(ages 3-5years old from kindergartens and day care centers), their parents, and their 51 teachers. The collected data were calculated and analyzed using SPSS 22.0 and AMOS 22.0. Results: Consisted of two components, two task types, 17 performance tasks, and 41 items in three dimensions. Rubrics were determined and based on the child’s best performance, and categorized into five levels. Lastly, the item difficulty and item discriminating power were defined in order to comprehend the item quality analysis, which showed that average scores varied depending on the performance. Conclusion/Implications: The musicality rating scale for young children is significant in order to comprehend musicality levels through the performances of children aged three to five. This study has educational implications in that teachers can connect the results of the ratings to curriculum and promote the development of teaching and learning methodologies based on the musicality levels of individual children.