Oxygen 함량에 따른 Cr-O-N 코팅막의 미세구조 및 기계적 특성에 관한 연구

윤준서(Jun Seo Yun),권세훈(Se Hun Kwon),박인욱(In-Wook Park),이정두(Jeong Du Lee),김광호(Kwang Ho Kim) 한국표면공학회 2009 한국표면공학회지 Vol.42 No.5

Cr-O-N coatings having different oxygen contents were deposited on Si wafer and SUS 304 substrates by an arc ion plating technique using Cr target in Ar/O₂/N₂ gaseous atmosphere. As increasing oxygen content in the coating, the microstructure of Cr-O-N coating changed from polycrystalline having NaCl structure to amorphous structure. Further increase of oxygen content resulted in phase transformation from amorphous to rhombohedral structure. From the variations of d value and average grain size, it was revealed that the maximum solubility of oxygen in Cr-O-N coating was about 21 at.%. And the maximum micro-hardness of 2751HK was obtained in this composition. The lowest friction coefficient was measured in the coating having 34.8 at.% of oxygen. However, more narrow width of wear track was found in the coating having 30.1 at.% of oxygen.

재조합 대장균으로부터 고순도 베타-카로틴의 추출 및 정제

조지송,엔구엔트구앙,윤준기,김유나,김유근,김성배,서양곤,이병학,강문국,김창준,Jo, Ji-Song,Nguyen, Do Quynh Anh,Yun, Jun-Ki,Kim, Yu-Na,Kim, You-Geun,Kim, Sung-Bae,Seo, Yang-Gon,Lee, Byung-Hak,Kang, Moon-Kook,Kim, Chang-Joon 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.24 No.3

재조합 대장균으로부터 고순도 베타-카로틴을 효율적으로 회수하기 위한 추출정제 방법을 개발하기 위하여 세포파쇄의 유무, 추출온도, 추출용매의 종류, 세포 대 추출용매의 비율 및 결정세척 용매의 선정이 베타-카로틴 추출수율과 순도에 미치는 영향을 조사하였다. 세포파쇄의 유무는 베타-카로틴 추출수율에 영향을 미치지 않았으므로 별도의 물리적 세포파쇄 단계가 생략되었다. 테스트된 용매 종류에 관계없이 $50^{\circ}C$에서 베타-카로틴의 추출수율은 $30^{\circ}C$에서보다 월등히 높았다. 아이소부틸 아세테이트에 의해 추출되는 베타-카로틴의 양은 추출성능이 제일 낮은 아세톤을 사용한 경우에 비해 7.6배 높았다. 0.4g의 동결건조세포로부터 베타-카로틴을 최대로 추출하기 위해 필요한 아이소부틸 아세테이트의 최적양은 10mL였고, 이는 단위 건조 세포(g-dry cells)당 25mL의 아이소부틸 아세테이트가 필요함을 의미하였다. 아세톤과 올리브오일을 동일비율로 혼합한 용액에 의한 추출양은 아세톤만을 사용한 경우보다 훨씬 높았고 가장 높은 값을 나타낸 아이소부틸 아세테이트에 의한 값과 비슷한 수치를 나타내었다. 추출액에 용해되어 있는 베타-카로틴의 결정 생성을 촉진시켜 베타-카로틴을 추출용액으로 분리하여 얻어진 베타-카로틴 결정의 순도는 89%으나 이를 에탄올로 세척 시 순도가 98.5%로 향상되었다. HPLC, 분광광도계 분석외에 추가적으로 질량분석을 수행함으로써 회수된 결정이 베타-카로틴임을 확인하였다. 이와 같은 결과는 본 연구팀에서 개발한 방법으로 식품의약 안전청에서 고시한 화장품 및 식품의 첨가물 기준을 만족시키는 고순도 베타-카로틴을 효율적으로 생산할 수 있음을 시사한다. This paper aimed to develop a solvent extraction and purification process to recover high-purified ${\beta}$-carotene from recombinant Escherichia coli. Cells harvested from the culture broth were treated through numerous steps: dehydration, solvent extraction, crystal formation and separation. To optimize the extracting condition, experiments were carried out to investigate the effect of cell disruption, temperature, organic solvents, solvent-biomass ratio on the yield of ${\beta}$-carotene extracted from cells. The result indicated that no significant differences of extraction yield were observed from cells with or without step of cell disruption. Among different extracting solvents, the highest extraction yield of ${\beta}$-carotene, 30.3 mg-${\beta}$-carotene/g-dry cells, was obtained with isobutyl acetate at solvent-biomass ratio 25 mL/g-dry cells at $50^{\circ}C$. Notably, in case of acetone, the extraction yield was quite low when using acetone itself, but increased almost up to the highest value when combining this solvent and olive oil. The purity of ${\beta}$-carotene crystals obtained from crystallization and separation was 89%. The purity degree was further improved up to 98.5% by treating crude crystals with additional ethanol washing.

발효, 세포배양, 생물공정 재조합 대장균으로부터 고순도 베타-카로틴의 추출 및 정제

조지송 ( Ji Song Jo ),엔구엔트구앙 ( Do Quynh Anh Nguyen ),윤준기 ( Jun Ki Yun ),김유나 ( Yu Na Kim ),김유근 ( You Geun Kim ),김성배 ( Sung Bae Kim ),서양곤 ( Yang Gon Seo ),이병학 ( Byung Hak Lee ),강문국 ( Moon Kook Kang ),김창준 ( 한국미생물생명공학회 2009 한국미생물·생명공학회지 Vol.37 No.3

일부 건강식품 섭취 실태 조사 연구

양윤준,서홍관 인제대학교 1992 仁濟醫學 Vol.13 No.2

소위 건강식품의 섭취실태를 보기 위해, 건강 검진 센터 이용자를 대상으로 설문 조사를 실시하였는데, 섭취 경험은 75%에서 있었고, 부작용은 4.2%가 경험하였으며, 의료진과 상의하여 섭취한 경우는 15.3%였다. In order to examine how many persons take the health food and what kind of side effects they encountered and the relationship between their life style and the intake of health food, this study was carried out through direct questionnaire to persons visiting Health Care Center in 4 hospitals in Seoul and Incheon from August 9 to October 15 in 1991. The number of total respondents was 631. The results are as follows ; 1.Among the 631 respondents, 463 were male(73.4%) and 167 were female(26.5%). Most respondents(81%) were between 3rd and 5th decade. Most female persons were between 20-and 24-year-old. 2.Among the respondents about the experience of taking health food(613), 479(75%) persons had the experience of intake and 134(25%) persons had not. The men who were educated in higher grade school or more careful for their health took the health food more frequently. 3.The reasons for the intake of the health food are 'for the prevention of disease'(27.3%), 'for relieving easy fatigability'(21.6%), 'by the motivation of near persons'(9.8%), 'because they had got it by presents'(8.6%), 'because they felt not healthy irrespective of normal clinical laboratory results'(7.0%), 'for treating some disease'(3.3%), 'because of the limitation of the modern medicine'(0.6%) and others(4.1%). 4.The discussion with medical personnel about taking the health food was present in fractionary population(15.3%). They took the health food largely by the motivation of a near persons(79.3%). 5.Most respondents felt that the effects of the health food were 'intermideate'(78.9%). Positive responses about the effect were 14.2%, and negative ones were 6.9%. 6.The ratio of dissatisfied respondents about the effect of health food was 19.3%. The reasons of dissatisfaction were uselessness(41.6%), high cost(25.8%) over-advertisement(19.2%), side effect(2.2%) in order of frequency. 7.Among 479 respondents who had took heaqlth food, 20 repondents(4.2%) experienced side effect of health flood. They experienced gastrointestinal disturbance in 10 casts, dermatologic problems in 8 cases, fever in 1 case and dizziness in 1 case.

우리나라 의과대학생의 흡연실태 및 흡연과 금연에 영향을 미치는 요소

양윤준,서홍관,최지호 대한가정의학회 1995 Korean Journal of Family Medicine Vol.16 No.2

본태성 고혈압에 대한 저용량 captopril 일일 일회 요법의 치료 효과에 관한 연구

김철환,서홍관,양윤준 인제대학교 1993 仁濟醫學 Vol.14 No.4

저자들은 반감기가 짧은 항고혈압제의 하나인 captopril을 1일 1회 투여하여 혈압의 조절에 어떤 효과를 보이는지를 알아보았다. ACE inhibitor is a effective, easily accepted anti-hypertensive drug of which side effects are infrequent and not serious. We observed 44 patients who had been treated with once-daily, low dose captopril for 12 weeks. The dose of captopril was 25 or 50mg per day. Among 44 patients. there were 28 patients(63.6%) whose diastolic blood pressure was lowered below 90 mmHg. But among another 16 patients, 14 patients should have been treated with larger amounts of captopril and 2 patients should have been changed to other drugs for the purpose of lowering the diastolic pressure below 90 mmHg. In order to find the factors that influenced the control of hypertension, we investigated several factors, such as the patient's age, sex, the period of hypertension, the amount of alcohol intake, Body Mass Index(BMI), and the level of intial blood pressure, but we didn't find any specific factors that had influenced the difference between the controlled group and the non-controlled group who had taken once-daily, low dose captopril(p>0.05).

Captopril의 특발성 부종 조절 효과에 대한 연구

김철환,서홍관,양윤준 인제대학교 1994 仁濟醫學 Vol.15 No.4

연구배경;가정의학과 외래 진료에서 흔하게 접하게 되는 부종 환자의 가장 흔한 원인은 특발성 부종(idiopathic edema)이다. 이뇨제가 아니고 엔지오텐신 전환효소 억제약물(Angiotensin converting enzyme inhibitor, ACE inhibitor)인 Captopril의 부종 조절 효과를 알아보기 위하여 연구를 시행하였다. 방법;1992년 11월 부터 1993년 8월까지 부종을 주소로 서울백병원 가정의학과 외래를 방문한 환자 77명을 대상으로 혈압 및 신체계측, 이학적 검사, 전혈검사(CBC), 혈중 BUN/Cr, Na/K, GOT/GPT, Albumin, HBsAg, TSH 등을 측정하고 뇨단백을 비롯한 요검사와 요현미경검경, 흉부엑스선검사를 실시하였다. 이러한 검사에서 부종을 일으킬만한 특별한 원인이 없고, 또한 하루 2회 체중을 측정하여 0.5 Kg 이상의 체중변화가 있다는 것이 확인된 21명 중 연구에 동의하고 끝까지 참여한 19명의 특발성 부종 환자를 연구대상으로 하였다. 이들을 진찰권 번호에 따라 무작위로 2개 그룹으로 나누어 한 그룹에는 Captopril 25mg을 아침 식전 투여하도록 하였고, 다른 한 그룹에는 Captopril의 모양과 색이 같은 위약(placebo)을 투여하였다. 부종조절효과는 하루 2회 2주간 체중을 측정하여 아침과 저녁 체중 차이가 줄어드는 정도를 지표로 판정하였다. 결과;특발성 부종으로 확진된 환자에서 Captopril과 위약(placebo) 모두 체중변화를 줄이는 경향은 있었으나 통계적으로 유의하지는 않았다. 결론;특발성 부종 환자를 대상으로 하루 25mg Captopril과 위약 투여 후 부종조절효과를 판정한 결과한 통계적으로 유의한 효과는 없었다. Background:Primary care physicians frequently meet the patients who complain of edema. We carried out this study to evaluate the captopril's effect on edema control. Methods:There were 77 patients who had suffered from various types of edema among the patients who had visited the Family Medicine Clinic Of Seoul Paik Hospital from November 1992 to August 1993. We took their medical history, perfomed physical examination, and requested chest X-ray and laboratory tests such as CBC, serum BUN /Cr, Na/K, GOT/GPT, Albumin, HBsAg, TSH, Urinalysis with microscopic examination. Thereafter we could select idiopathic edema. And we prescibed captopril(25mg once a day at morning) for case group, and placebo for control group. We evaluated the captopril's effect on edema through comparing the diurnal change of body weight between case group and control group by paired t -test. Results:Among 77 patients, women were 67 persons(87.0%). There were 13 patients(16.9%) who had organic problems such as chronic renal failure, liver disease, hypothyroidism, and so on. There were 20 healthy people(31.3%) complaining of edema whose range of diurnal body weight change was over 0.5 kg, 9 healthy ones (14.0%) complaining of edema whose range of diurnal body weight change was below 0.5 kg, and 35(54.7%) healthy people complaining of edema whose body weight was not checked. We evaluated the captopril's effect on edema through comparing the diurnal change of body weight between case group and control group but there was no statistically significant difference between case group and placebo group. Conclusions:There were 16.9% organic causes among the patients who had complained of edema. We prescribed captopril to control of enema in case of idiopathic edema, but it was not effective.