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유은숙(Eun Sook Yoo),김옥희(Ok Hee Kim),손여원(Yeo Won Son),정인경(In Kyung Jung),이상섭(Sang Sup Lee) 대한약학회 1999 약학회지 Vol.43 No.1
To investigate analgesic mechanism of capsaicin and its analogues (capsaicinoids), adenosine release was measured by high performance liquid chromatography from rat spinal cord synaptosomes. Exposure of synaptosomes to K+ and morphine Produced a dose dependent release of adenosine in the presence of Ca++. Capsaicin (0.1, 1, 10mcM), and its analogues; NE-19550 (1, 10, 100mcM), DMNE (1, 10, 100mcM) and KR 25018 (0.1, 1, 10mcM) produced a concentration dependent release of adenosine in the presence of Ca++. Nifedifine, L-type voltage sensitive calcium channel blocker, inhibited K+ (6, 12mM)- and morphine (10mcM)-evoked release of adenosine partially. Capsazepine, a novel capsacin selective antagonist, blocked only capsaicin and capsaicinoids induced release of adenosine. Therefore, it is suggested that the adenosine release by capsaicin and capsaicinoids having antinociceptive effects involves activation of capsaicin specific receptor and capsaicin sensitive Ca++ channel.
흰쥐 척수에서 지속성 진통물질 6-파라돌에 의한 아데노신의 유리 증가
유은숙(Eun Sook Yoo),김옥희(Ok Hee Kim),이상섭(Sang Sup Lee) 대한약학회 2000 약학회지 Vol.44 No.6
We previously demonstrated that 6-paradol, a compound structurally related to capsaicin, showed to produce prolonged analgesia in experimental animals. The effects of 6-paradol on the release of adenosine were investigated in the rat spinal cord synaptosomes by high performance liquid chromatography. In the presence of Ca++ adenosine was released from synaptosomes of rat spinal cord by 6-paradol and capsaicin in a dose dependent manner. Nifedifine, L-type voltage sensitive calcium channel blocker, was found to be ineffective in releasing adenosine by 10mcM 6-paradol. After exposure to 10mcM capsazepine, a novel capsaicin selective antagonist, the level of adenosine evoked by 10mcM 6-paradol was decreased by 75%, and that evoked by 10mcM capsaicin was blocked completely. These results suggest that the analgesic effect of 6-paradol might be mediated by the vanilloid (capsaicin) sensitive pathway, or the direct binding to the vanilloid receptor.
생강성분 6-Paradol의 세포 독성 및 병리학적 연구
김옥희,유은숙,정인경,이상섭,Kim, Ok-Hee,Yoo, Eun-Sook,Jung, In-Kyung,Lee, Sang-Sup 한국환경성돌연변이발암원학회 1998 한국환경성돌연변이·발암원학회지 Vol.18 No.1
It is previously reported that 6-paradol can induce prolonged analgesia in experimental animals. In order to investigate the mutagenicity of 6-paradol, Ames Samonella/microsome plate assay was carried out with Salmonella typhimurium strains, TA 98, TA 100, TA 1535 and TA 1538, 6-Paradol was nonmutagenic in Salmonella typhimurium with and without rat liver microsomal activation. The rec assay with Bacillus substilis strains H 17 $rec^+$ and M 45 $rec^+$ was carried out ot test 6-paradol and other compounds (1-3 mg/disc) for DNA damaging activity, 6-Paradol was also nonmutagenic in DNA damaging activity. The relative size of the inhibition zone for 6-paradol was smaller than that of capsaicin. We have also determined the pathological effects of this compound on the various tissues of rats after administrating(i.p.) with increasing doses of 4, 8, 12, 16 mg/kg at 2 hour intervals and found no significant changes in terms of histology.
S. abortus 유래 LPS와 E. coli 유래 LPS에 의한 패혈증성 쇽 유도 작용 비교
조재열,유은숙,Cho, Jae-Youl,Yoo, Eun-Sook 대한약학회 2007 약학회지 Vol.51 No.1
Acute septic shock is one of inflammatory diseases mediated by pro-inflammatory cytokines such as tumor necrosis factor (TNF)-${\alpha}$. In this study, we examined the pathological difference and mechanism of lipopolysaccharides isolated from E. coli (E-LPS) or S. abortus (S-LPS) on inducing acute septic shock in ICR mouse. All mice were died by intraperitoneal treatment of S-LPS with 0.75 mg/kg, whereas E-LPS treated with even 3 mg/kg only showed 30% of mice lethal, indicating that S-LPS may be more feasible in triggering a strong septic shock condition. The secretion pattern of TNF-${\alpha}$, a critical pro-inflammatory cytokine in septic shock condition, was also distinct between E-LPS- and S-LPS-treated groups. Thus, S-LPS strikingly increased serum level of TNF-${\alpha}$ (6 ng/ml) at 1 h, while E-LPS just displayed at 2 ng/ml level. However the interaction of S-LPS with LPS receptor toll like receptor (TLR)-4, was not stronger than that of E-LPS, according to experiments with macrophage cell line RAW264.7 cells. Thus, E-LPS rather than S-LPS strongly enhanced the production of TNF-${\alpha}$. Interestingly, S-LPS more strongly up-regulated splenocyte proliferation, compared to E-LPS group, whereas there was no difference between S- or E-LPS treated groups in proliferation of Balb/c- or C57BL/6-originated splenic lymphocytes. Therefore, our data suggest that S-LPS is a more active endotoxin and that the strong septic shock-inducing effect of S-LPS seems due to the enhancement of early TNF-${\alpha}$ production and S-LPS-sensitive lymphocyte proliferation.
문주란 추출물의 HL-60 백혈병 세포 Apoptosis 유도 효과
현재희,김엘비라,강정일,김상철,유은숙,강희경,Hyun, Jae-Hee,Kim, El-Vi-Ra,Kang, Jung-Il,Kim, Sang-Cheol,Yoo, Eun-Sook,Kang, Hee-Kyoung 대한약학회 2008 약학회지 Vol.52 No.1
The present study investigated the antiproliferative effects of Crnum asiaticum var. japonicum against HL-60 human leukemia cells. The 80% MeOH extract or several solvent fractions from the C. asiaticum inhibited the growth of HL-60 cells, whereas the growth of HEL-299 cells, human embryonic lung fibroblast, was scarcely inhibited. When the HL-60 cells were treated with the $CHCl_3$ fraction, the BuOH fraction, the EtOAc fraction and the $H_2O$ fraction, DNA ladder, chromatin condensation and increase of sub-G1 hypodiploid cells were observed. Furthermore, the $CHCl_3$ fraction and the BuOH fraction reduced Bc1-2 mRNA level, whereas Bax mRNA level was increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60 cell might be mediated through the induction of apoptosis via the down-regulation of Bc1-2. Taken together, components of C. asiaticum might have a therapeutic potential for the treatment of human leukemia.
대리만족에 대한 부모의 욕구가 자녀의 인격형성에 미치는 영향에 관한 연구
이지훈(Ji-Hun Lee),유은숙(Eun-Sook Yoo) 한국엔터테인먼트산업학회 2019 한국엔터테인먼트산업학회 학술대회 논문집 Vol.2019 No.5
학부모는 그칠 줄 모르는 교육열이라는 동력을 가지고 자녀교육에 직접 개입하면서 교육에 지속적이고 강력한 영향력을 미치는 존재로 인식되고 있다. 따라서 본 연구는 고액과외, 엄친아, 과시욕, 체벌, 기대치, 압박감이 인격형성에 미치는 영향관계를 통해 자녀의 인격형성에 미치는 시사점을 제시하고자 한다. 분석은 표본추출방법인 비확률 추출방법 중 판별 표본 추출법에 의해 조사를 할 것이다. 수집된 유효 표본은 판별 타당성과 수렴 타당성의 내적 타당성 확보를 위해 확인적 요인분석을 이용하여 타당성을 검정할 것이다. 마지막으로 변수들 간에 인과관계를 분석할 것이다. Parents are perceived to have a lasting and powerful influence on education, directly intervening in their children"s education with the unstoppable zeal for education. therefore. This study aims to present implications for the formation of a child"s character through the influence of extracurricularity, eomchina, flaunting, corporal punishment, expectations and pressure on personality formation. The analysis will be investigated by the plate sampling method among sampling methods, non-probability sampling methods. The effective samples collected will be validated using a validated factorial analysis to ensure internal validity of the legality and convergence validity. Finally, we will analyze causality among variabl.
까마귀쪽나무(Litsea japonica)의 HL-60 백혈병 세포 Apoptosis 유도효과
김엘비라,부혜진,현재희,김상철,강정일,김민경,유은숙,강희경,Kim, Elvira,Boo, Hye-Jin,Hyun, Jae-Hee,Kim, Sang-Cheol,Kang, Jung-Il,Kim, Min-Kyoung,Yoo, Eun-Sook,Kang, Hee-Kyoung 대한약학회 2009 약학회지 Vol.53 No.1
This study investigated the antiproliferative effect of the EtOH extract from Litsea japonica. The extract markedly inhibited the growth of HL-60 cells. When treated with the extract, several apoptosis events like as DNA fragmentation, chromatin condensation and the increase of the population of sub-G1 hypodiploid cells were observed. The extract decreased the Bcl-2 expression, whereas the Bax expression was increased. Caspase-9 and -3 were activated and poly (ADP-ribose) polymerase was cleaved. The results suggest that the antiproliferative effect of L. japonica in HL-60 appears to arise from apoptosis induction via the down-regulation of Bcl-2 and the activation of caspases.
Docosahexaenoic acid의 모유두세포 증식 효능 및 기전
고지연,오일중,강정일,최윤경,윤훈석,유은숙,고창익,안용석,Ko, Jiyeon,Oh, Il-Joong,Kang, Jung-Il,Choi, Youn Kyung,Yoon, Hoon-Seok,Yoo, Eun-Sook,Ko, Chang-Ik,Ahn, Yong-Seok 제주대학교 의과학연구소 2019 The Journal of Medicine and Life Science Vol.16 No.3
Docosahexaenoic acid (DHA), a principal of mackerel-derived fermented fish oil, increases the proliferation of dermal papilla cells (DPCs) via the upregulation of cell cycle-associated proteins such as cyclin D1 and cdc2 p34, and might promote hair-growth. However, the intracellular mechanisms that underlie the action of DHA in the proliferation of DPCs have not been investigated fully. In this study, we addressed the action mechanisms of DHA to trigger the activation of anagen in DPCs. DHA activated β-catenin signaling by the increased phosphorylation at serine 552 and serine 675 as well as the translocation and accumulation of activated β-catenin into the nucleus. In the other hand, DHA inhibited canonical TGF-β/Smad signaling by the decreased phosphorylation of Smad2/3. Taken together, the results indicate that DHA might stimulate anagen signaling via the activation of Wnt/β-catenin pathway, while the inactivation of canonical TGF-β signaling pathway in DPCs.