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자궁경부암 세포의 증식에 관여하는 annexin-I의 역할
유영옥(Young Ok Lew),박태철(Tae Chul Park),남궁성은(Sung Eun NamKoong),박동춘(Dong Choon Park),김재훈(Jae Hoon Kim),김대훈(Dae Hoon Kim),양용재(Yong Jae Yang),이지현(Jee Hyun Lee),조성대(Sung Dae Cho),조형권(Hyeong Kwon Jo) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.10
목적: 인간 자궁경부암에서 annexin-I의 역할을 규명하기 위해 annexin -I의 발현과 암세포증식과의 상관관계를 조사하였다. 방법: 면역조직화학 (immunohistochemical) 염색법 과 Western blotting으로 자궁경부암 조직에서 annexin-I 발현의 정도와 분포를 조사하였다. 악성세포주인 SiHa와 HeLa 세포주를 tamoxifen, β-estradiol과 all-trans retinoic acid로 3일 또는 5일간 처리한 후 각 세포의 증식을 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) colorimetric assay로 측정하였고 동시에 유세포측정기(flow cytometry)로 annexin-I의 발현을 조사하였다. 결과: Annexin-1에 대한 면역조직화학 분석 결과 자궁경부암세포가 진한 과립상으로 염색되어 관찰되었으며, Western blotting 분석에서는 annexin-I의 발현이 정상이나 양성조직보다 암조직에서 증가하였다. 한편, tamoxifen, β-estradiol 또는 all-trans retinoic acid와 같은 세포증식 조절물질을 이용한 세포주 실험에서는, tamoxifen이나 β-estradiol로 SiHa와 HeLa 세포주를 증식시킨 뒤 annexin-I의 발현이 증폭되었고 all-trans retinoic acid에 의한 항증식 후 annexin-I의 발현이 감소되었다. 결론: Annexin-I의 발현은 정상조직보다 자궁경부암에서 증가되어 있었고 자궁경부암 세포주의 증식과 비례해서 annexin-I의 발현이 증가되었다 Objectives: To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. Results: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and β- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. Conclusions: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.
정대영(Dae Young Chung),신종철(Jong Chul Shin),백은정(Eun Jung Baik),이영(Young Lee),유영옥(Young Oak Lew),이종건(Jong Kun Lee),김창이(Chang Yi Kim),김대훈(Dae Hoon Kim),김수평(Su Pyung Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12
N/A The incidence of ectpic pregnancy has increased rapidly over the past decade. Currently, the complications of this disorder make it one of the leading causes of maternal mortality and morbidity. In the late 1980s, reports of successful use of methotrexate (MTX) as a non surgical treatment for ectopic pregnancy (EP) also began to appear in the literature. With MTX emerging as a possible alternative management of selected women with an EP, an analysis of its comparative costs is now needed. We examined retrospectively the costs associated with management of EP in our hospital. We selected 30 women with EPand divided them into two groups of MTX treated group (n= l0) and surgically treated group (n=20). There were no statistically significant differences in the doctors fee, room, charge and cost of laboratory and radiologic examination between two groups. But We found significant reduction in cost with regard to pharmacy, injection, procedure and operation of MTX treated group compared to surgically treated group (p<0.05). Consequently the use of MTX for EP should still be considered investigational; we recognize and agree that the evolution of this remedial option should be based primarily on its ability to decrease morbidity and mortality rather than on its ability to reduce costs. If medical treatment proves to be effective, the potential cost savings demonstrated in this report will become an important influential factor on selecting alternative remedy.
임신능이 확인된 가임 여성에서의 Deciduosis의 유병율
김미란,유영옥,노덕영,류순원,권동진,김장흡,김진홍,임용택,김은중,정재근,이진우,Kim, Mee-Ran,Lew, Young-Oak,Ro, Duck-Yeong,Ryu, Sun-Won,Kwan, Dong-Jin,Kim, Jang-Heub,Kim, Jin-Hong,Lim, Yong-Taik,Kim, Eun-Jung,Jung, Jae-Keun,Lee, Jin-Woo 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4
Objective: Extrauterine formation of decidua of stromal cells has been well described, particularly in the cervix and ovary. The apparent hormonal mechanisn of this phenomenon suggestes a relationship to endometriosis. Whether formation of ectopic decidua represents a marked progestational response of endometriosis or an independent peritoneal-stromal reaction to pregnancy is unclear. This study was designed to determine the prevalence of deciduosis in the patients whose fertility were proven. Design: Prospective study of patients who had undergone cesarean delivery without history of endometriosis. Materials and Methods: The study was performed in 179 full tenn pregnant women. During the cesarean section, the pelvic organs were thoroughly investigated and the biopsies were collected at the lesions suspicious endometriosis. And then microscopic examination of removed tissues were done. Results: Of the 179 patients who underwent cesarean delivery, 48 women (26.8%) had the lesions suspicious endometriosis such as adhesion, pigmented spots. The ovary was the most frequently ocurred site (79.2%). Microscopically, decidual cells were observed in 34 cases (70.8%) of 48 biopsed patients. Conclusion: Endometriosis has been known to be associated with subfertility. Our observations found the prevalence of deciduosis was 19.0% (34/179) in tenn pregnant women whose fertilites were proven. We suggests that the deciduosis maya manifestation of endometriosis during pregnancy. However, further follow up study should be done to confirm this clinicopathologic process.
이혜영 ( Hye Young Lee ),박동춘 ( Dong Choon Park ),김성환 ( Sung Whan Kim ),유영옥 ( Young Ok Lew ),김대훈 ( Dae Hoon Kim ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.4
목적 : 자궁육종 세포주에서 Taxol의 세포독성 및 방사선 감수성 증진 효과에 대해 알아보았다. 연구 방법 : 자궁육종 세포주 중 다제 내성 (multidrug-resistance. MDR) 유전자를 갖고 있지 않은 MES-SA와 이 유전자를 갖고 있는 MES-SA/MX2에서 10-8 M 농도의 Taxol, 10 Gy의 방사선 조사 및 같은 농도의 Taxol과 방사선을 함계 투여하였을 때의 세포증식의 변화를 XTT 분석 및 Tunnel study로 조 Objective : The cytotoxic and radiosensitizing effects of Taxol in uterine sarcoma cell lines were investigated. Methods : Two uterine sarcoma cell lines with different Taxol responses were used, namely, Taxol-sensitive and MDR gene negative MES-SA, and T
임신오조증으로 발생한 Wernicke`s Encephalopathy 1 예
김수평(Soo Pyung Kim),유영옥(Young Oak Lew),김대훈(Dae Hoon Kim),양용재(Yong Jae Yang),이형근(Hyung Gun Lee),임채춘(Chae Chun Rhim),나순숙(Soon Sook Nah),박동춘(Dong Chun Park),정대영(Dae Young Jeong),김재훈(Jae Hoon Kim) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.1
Hyperemesis gravidarum is a complication defined as nausea and vomiting during early pregnancy. Wernickes encephalopathy is an illness of acute onset characterized by mental disturbance, paralysis of eye movements, and ataxia of gait cause by thiamine deficiency. We recently experienced a case of Wernickes encephalopalhy due to hyperemesis gravidarum, which is treated by administration of thiamine even though outcome of serious healthy problem to both mother and fetus, and reported it with a brief review of the literature.
성선 자극 호르몬과 사이토카인이 인간 황체 세포의 아포프토시스에 미치는 영향
김민정 ( Min Joung Kim ),허수영 ( Soo Young Hur ),유영옥 ( Young Oak Lew ),권동진 ( Dong Jin Kwon ),김장흡 ( Jang Heub Kim ),이진우 ( Jin Woo Lee ),이원선 ( Weon Sun Lee ),박상희 ( Sang Hi Park ),김은중 ( Eun Jung Kim ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.3
목적 : 초기 황체기 동안에 성선 자극호르몬 (gonadotropin)과 사이토카인 (cytokine)이 황체 세포의 아포프토시스 (apoptosis)에 미치는 영향과 아포프토시스 관련 인자인 Fas, Fas-L, Bcl-2, Bax, p53과 caspase-8의 발현 변화 및 상호 관련성을 조사하여 황체의 성장과 퇴화에 관여하는 기전의 단서를 알아내고자 하였다. 연구 방법 : 황체 세포를 배양액만으로 24시간 배양한 것을 대조군으로 하였고, 다양한 농 Objective : Our object is to evaluate the detailed mechanisms of support and regression of the human corpus luteum. Methods : To investigate the regulation of luteal function by gonadotropins, cytokines, and prostaglandins, the frequency of apoptosis and
폐경 후 여성에서 혈청 렙틴 농도와 골밀도 및 골표지자와의 연관성
이석우 ( Suk Woo Lee ),김미란 ( Mee Ran Kim ),권동진 ( Dong Jin Kwon ),유영옥 ( Young Oak Lew ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.12
목적: 폐경 후 여성에서 혈청 렙틴과 요추부, 대퇴경부의 골밀도, 골표지자와의 상관관계를 알아보고자 본 연구를 시행하였다. 방법: 2007년 3월 1일부터 2007년 12월 31일까지 가톨릭대학교 성빈센트병원 산부인과를 방문한 폐경기전 여성 88명, 폐경기 후 여성 118명을 대상으로 혈청 렙틴과 체질량지수, 요추부, 대퇴경부의 골밀도, 골표지자인 혈청 osteocalcin과 요 중 deoxypyridinoline과의 관계를 상관분석과 회귀분석을 통해 관찰하였다. 결과: 혈청 렙틴의 농도는 체질량지수의 증가에 따라 양의 상관관계를 나타내며 통계적으로 유의한 차이가 있었으나 폐경 전 여성에서 요추부, 대퇴경부의 골밀도와의 연관성은 없었고, 폐경 후 여성에서는 양의 상관관계를 보였으나 골밀도에 영향을 미치는 나이와 체질량지수를 보정하였을 때 통계적으로 유의한 연관성은 없었다. 골표지자인 혈청 osteocalcin과 요 중 deoxypyridinoline과의 상관관계도 통계적으로 유의한 차이는 없었다. 결론: 혈청 렙틴은 골량의 조절에 있어 골형성의 촉진과 억제 두 가지 기능을 모두 보이며, 이로 인해 혈청 렙틴과 골밀도 및 골표지자와의 직접적인 상관관계는 없는 것으로 연구되었다. Objective: This study was performed to prove the relationship between serum leptin level and bone mineral density of lumbar spine, femur neck and bone markers in postmenopausal Korean women. Methods: We measured serum leptin, serum osteocalcin, urine deoxypyridinoline levels and bone mineral density of lumbar spine, femur neck in 88 premenopausal and 118 postmenopausal women who visited St. Vincent Hospital of Catholic University of Korea from March 1st, 2007 to December 31th, 2007. Results: Statistically significant correlation was shown between serum leptin level and body mass index (BMI) in both premenopausal (r=0.343, P<0.0001) and postmenopausal women (r=0.360, P<0.0001). And no significant correlation was observed between serum leptin level and bone mineral density (BMD) of lumbar spine and femur neck in premenopausal women (r=0.013, P=0.107 and r=0.004, P=0.425, respectively), but in postmenopausal women, there was a positive correlation between serum leptin and lumbar spinal BMD (r=0.085, P=0.02). But after the adjustment with age and BMI, the serum leptin and BMD of lumbar spine did not showed a significantly correlation in the same group (r=0.088, P=0.939). Also, no significant correlations were observed between serum leptin level and serum osteocalcin and urine deoxypyridinoline in premenopausal (r=0.004, P=0.566 and r=0.002, P=0.707, respectively) and postmenopausal women (r=0.026, P=0.096 and r=0.000, P=0.933, respectively). Conclusions: In our study, there is no significant correlation between serum leptin level and bone mineral density and bone markers in premenopausal and postmenopausal Korean women. Our own data would suggest that leptin has both negative and positive effects in bone mass regulation. Furthermore, larger clinical studies are necessary to clarify leptin`s role to assess the contribution of the central and peripheral role of leptin in the overall maintenance of bone turnover.