촛불에 찬성하면 진보, 반대하면 보수?

연규선 관훈클럽 2008 관훈저널 Vol.- No.108

염색체 이상에 의한 반복 유산 환자에서 체외수정시술 및 착상전 유전진단을 통한 임신 성공 1례

정진석,연규선,채희동,전용필,김정훈,강병문,장윤석,목정은,Jeong, Jin-Seok,Yeon, Gyu-Sun,Chae, Hee-Dong,Cheon, Yong-Pil,Kim, Chung-Hoon,Kang, Byung-Moon,Chang, Yoon-Seok,Mok, Jung-Eun 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

It was reported that the etiologies of recurrent spontaneous abortion are immunologic factors, endocrinologic problems, anatomical abnormalities, genetic abnormalities, infection, and unexplained factors. Among those etiologic factors, genetic abnormalities occur in about 5% of the couples who experience recurrent spontaneous abortions, and most common parental chromosomal abnormality contributing to recurrent abortion is balanced translocation. The advent of in vitro fertilization (IVF), the development of skills associated with the handling of human embryo, and an explosion of knowledge in molecular biology have opened the possibility of early diagnosis of genetic disease in preimplantation embryos. Therefore preimplantation genetic diagnosis (PGD) is indicated for couples, infertile or not, at risk of transmitting a genetic disease. A case of successful pregnancy and term delivery by PGD using fluorescence in situ hybridization (FISH) technique in patient with recurrent spontaneous abortion due to balanced translocation is presented with brief review of literatures.

광전용적맥파를 이용한 요골동맥 맥파 검출

정동근,김광년,연규선,최병철,서덕준 한국생명과학회 2003 생명과학회지 Vol.13 No.1

광전용적맥파는 인체의 말초 부위에서 맥파를 검출하는데 널리 이용되고 있으며 심박동수 측정 및 혈액산소포화도 측정에 이용되고 있다. 본 연구는 940 nm의 적외선을 이용한 광전용적맥파를 이용하여 손목에서 요골동맥의 맥파를 기록하였으며 센서의 측정 위치에 따라 맥파의 모양이 다르게 나타나는 것을 관찰하였다. 요골동맥 바로 위의 피부부위에서 기록되는 광전용적파는 손가락 끝에서 기록되는 맥파에 비하여 반전되어있는 특성을 보였다. 반전된 맥파의 발생기전은 센서의 발광부에서 나오는 940 nm의 적외선이 조직에 입사하여 반사되어 나오는 경로의 용적 변화가 심장주기에 따라 일어나지만 손가락 끝부분에 비하여 반대 작용이 있음을 나타낸다. 요골동맥의 바로 위 피하 조직은 심장주기에 따라 동맥의 확장 또는 수축이 발생하면 동맥외벽과 피부 사이 조직에 혈류량의 변화가 일어나며 동맥의 확장 시에는 혈류량이 감소하고 이완시에는 증가되는 특성으로 인하여 반전맥파를 형성하는 것으로 추측된다. 이러한 결과는 요골동맥의 벽에서 적외선 반사가 있음을 시사한다. Photoplethysmogram is widely used to measure heart rate and arterial blood oxygen saturation in human. This paper describes radial pulse waves recorded by photoplethysmogram with 940 nm wavelength infra red light. Radial pulse waves were varied according to the recording site. When recorded on the skin over radial artery, the radial pulse wave was inverted, comparing to the photoplethysmogram at fingertip. The mechanism of inverted pulse wave seemed to be caused by the change of the blood volume in the subcutaneous tissue between radial artery and the skin, which was reduced during systolic period and increased during diastolic period of the cardiac cycle. These results suggest that radial arterial wall may reflect infra red ray.

8 세 여아에서 발생한 난소 윤상 소관 성기삭 종양의 복강경수술 치험 1 예

김종혁,목정은,나준희,연규선,홍승모,김온자 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.7

저자 등은 8세 여아의 좌측 난소에 발생한 윤상 소관 성기삭 종양(sex cord tumor with annular tubules) 1예를 복강경 수술로서 치험하였기에 문헌 고찰과 함께 보고하는 바이다. The sex cord-stromal cell tumors can be subclassified into granulosa-stromal cell or Sertoli-Leydig cell tumors according to the patterns of growth and the appearance of the neoplastic cells. However there has been difficulties in the interpretation of minority of these neoplasms due to their intermediate patterns and cell types. The sex cord tumor with annular tubules (SCTAT) has morphologic features intermediate between those of the granulosa cell tumor and those of the Sertoli cell tumor, and characterized by the presence of structures of ring-shaped tubules. Peutz-Jeghers syndrome and adenoma malignum of the cervix have been sometimes accompanied in patients with SCTAT. The symptoms are frequently correlated with kinds of hormone secreted by the tumors. We experienced a case of SCTAT without Peutz-Jeghers syndrome in a 8-year old girl with breast enlargement as a presenting manifestation and the laparoscopic unilateral oophorectomy was performed with no obvious complications. We report this case with brief review of literatures.

융모막 융모 및 임신성 융모성 질환에서 bcl-2 단백의 발현에 관한 연구

김종혁,목정은,김용만,김영탁,남주현,나준희,허주령,연규선,공훈식 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.9

저자 등은1990년 1월부터 1996년 12월까지 울산대학교 의과대학 서울중앙병원 산부인과에서 치료받은 34예의 임신성 융모성 질환 환자 조직, 10예의 임신 조직 및 4예의 정상 자궁내막 조직을 대상으로 하여 bcl-2 단백의 면역조직학적 염색을 실시하여 그 발현을 확인한 결과, bcl-2 단백이 정상 임신 제1삼분기와 임신성 융모성 질환 모두에서 최종 분화 단계의 세포인 합포영양모세포에서 주로 발현하며, 정상 임신 조직보다 종양성(neoplastic) 태반, 즉 임신성 융모성 질환 조직에서 그 발현이 약하고 포상기태, 침윤성 기태, 융모막암 및 태반부착부 융모성 종양의 차례로 종양의 악성도가 높을수록 유의하게 낮은 발현율과 약한 발현도를 보이는 것으로 보아 종양유전자로서의 bcl-2 유전자는 임신성 융모성 질환의 발생기전에서 별다른 역할이 없고 단지 종양화의 결과에 따라 발현이 낮아진 것으로 판단되나 bcl-2 유전자가 임신성 융모성 질환에서는 종양억제 기능을 할 수 있다는 점도 완전히 배제할 수는 없기 때문에, 향후 DNA 또는 mRNA 수준에서의 계속적인 연구를 해 볼 가치가 있다고 사료된다. Bcl-2 is a proto-oncogene that inhibits apoptosis induced by hormones and cytokines and thus extends cell survival, exposing the cell to oncogenic stimuli. It is expressed in various normal tissues and neoplasia, often associated with prognostic significance. In normal placenta, only syncytiotrophoblasts have been found to express bcl-2. The role of bl-2 in gestational trophoblastic disease (GTD) is largely undetermined. In this study, the authors examined the possible role of bcl-2 in oncogenesis of GTD. Bcl-2 protein in 34 cases of GTD was examined by immunohistochemical staining on routinely processed paraffin-embedded tissues, using antihuman mouse monoclonal antibody according to standard streptavidine biotin method. Cases included 21 hydatidiform moles (H-mole), 7 invasive moles, 4 choriocarcinomas and 2 placental site trophoblastic tumors (PSTT). As controls, we included 5 first trimester pregnancy, 5 abortions and 4 normal endometrium. In normal first trimester placental tissues, distinct strong diffuse cytoplasmic staining for bcl-2 was observed only in syncytiotrophoblastic cells, but not in cytotrophoblasts or intermediate trophoblasts. The reaction was stronger than in gestational endometrial glandular cells. In H-moles, diffuse cytoplasmic staining of syncytiotrophoblasts was noted in 17 cases (81.0%). The reaction was similar to, or weaker than normal in placental tissue. In invasive moles, weak to moderate staining was noted in trophoblasts invading myometrium in 3 cases (42.9%). In choriocarcinomas, weak cytoplasmic stain was seen in syncytiotrophoblasts in 1 cases (25.0%). Two case of PSTT showed diffuse cytoplasmic staining in some syncytiotrophoblasts and intermediate trophoblasts. There is a significant inverse relationship between the expression of bcl-2 and the aggressiveness of GTD. This study shows that the exclusive localization of bcl-2 in syncytiotrophoblasts is well maintained in hydatidiform moles and invasive moles, whereas almost absent staining is seen in choriocarcinoma and PSTT. Inverse relationship between bcl-2 expression and aggressiveness of GTD suggests that other molecular events are required for tumorigenesis of GTD, by which expression of bcl-2 can be altered or bcl-2 function as a tumor suppressor gene depending on cell type.

경량 폴리머 콘크리트의 특성연구

채경희 ( Chae Kyung Hee ),최예환 ( Choi Ye Hwan ),연규선 ( Yeon Kyu Seok ),이윤수 ( Lee Youn Su ) 한국농공학회 2001 韓國農工學會誌 : 전원과 자원 Vol.43 No.4

Recent advance in material technology has accelerated the development of high strength concrete using lightweight artificial aggregates. The lightweight concrete has many advantages that the reduction of dead loads and the increase in load capacity can offer. In this study the lightweight polymer concrete using unsaturated polyester resin and lightweight aggregate were prepared and tested for testing the physical and the mechanical properties. The compressive strengths of lightweight polymer concretes with apparent specific gravity for 1.32 to 1.78 were 250 to 470 kfg/cm² and flexural strengths were measured to be in the range of 1/3-1/4 of compressive strength.