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안교필,김동원,김형환,최호영 대한한방성인병학회 2000 韓方成人病學會誌 Vol.6 No.1
Objectives : To discover and test the efficacy of new angiogenic materials, The angiogenic effect of the water extract of Ganoderma lucidum (EGL) was measured under rat sponge model for quantitative in-vivo studies. Methods : It was based on subcutaneous implantation of sterile polyester sponges in the male sprague dawley (SD) rats and we have characterized a dye method and histology. The angiogenic response was verified by quantitation of histological evaluation of vascularized sponges. Angiogenesis was assessed by the dye method that incorporate 5% carmine red (490 nm), 1% cresyl violet (609 nm) in 10% gelatin solution. It was used to monitor at fixed time points (days 7 and 14). vascularity index expressed as ㎍ dye/㎎ dry tissue. Results : Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the neovascularization between days 7 and 14. Daily doses of 1 μ㎎/㎏ and 10 μ㎎/㎏ EGL produced no apparent effect on spongeinduced angiogenesis but 100, 300 and 1000 ㎍/㎏ EGL caused intense neovasculalization. The vasoconstrictor peptide, angiotensin Ⅱ, in daily doses of 100 nmol, caused an intense neovascularization but lower doses of angiotensin Ⅱ produced no apparent effect (1 nmol, 10 nmol). 100 ㎍/㎏ EGL - induced angiogenesis represents a special form of angiogenesis and We have shown that co-administration of 100 ㎍/㎏ EGL and 100 nmol All accelerated potent angiogenesis. Conclusion : Thus we concluded that the EGL was possessed intense angiogenesis and EGL may have therapeutic potential for the management of endometrial development, wound repair and embryonic development.
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分心氣飮으로 治癒된 退行性 椎間板脫出症 患者의 治驗1例
김동원,안교필,이시섭,송경섭 대한한방성인병학회 2000 韓方成人病學會誌 Vol.6 No.1
Objectives : To report one case where Herniation of an Interverterbral Disc(HIVD) in operation indication was treated by Bunsimki-um. Methods : The changes in clinical symptoms of low back pain dysuria, HTN, etc. were described when this patient was treated with herb-medications of Bunsimki-um, etc. and with Acupuncture treatment. Results : Symptoms (low back pain, dysuria, HTN, etc) at admission decayed gradually with Bunsimki-um. The patients could walk alone at discharge. Conclusions : Though she had been in op. indication, the patient's health improved by herbmedications of Bunsimki-um, etc. and by Acupuncture treatment.
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이시섭,김동원,안교필,엄효진,남영 대한침구학회 2001 대한침구의학회지 Vol.18 No.6
Objective : To investigate the effects of Acupuncture therapy on the migraineur by way of the change of the Cerebral blood flow. Methods : 6 migraineurs were inserted in acupoints on GV16, G20, S8 during 2 weeks. and, Mean Velocity (MV) and Pulsatility Index (PI) of MCA, PCA, ACA, BA were measured before and after treatments of 2 weeks, respectively. Results : MV of MCA of the migraineurs was decreased very much by 13.3%, and P1 was decreased much by 10.4%. However, MV and PI of BA changed little. Conclusion : It is observed that Acupuncture therapy reduces pains of migraineur through decreasing Cerebral blood flow like MCA.
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