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- 저자 : 송견지(Gyun Jee Song) (검색결과 3 건)
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한국 남성 불임환자에서 Y 염색체상의 AZF Gene에 대한 분석 및 DAZ Gene의 발현 양상
이호준,이형송,송견지,변혜경,서주태,김종현,이유식,Lee, Ho-Joon,Lee, Hyoung-Song,Song, Gyun-Jee,Byun, Hye-Kyung,Seo, Ju-Tae,Kim, Jong-Hyun,Lee, You-Sik 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1
Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.
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동결보존이 생쥐 난소 조직 내 Heat Shock Protein 90의 발현에 미치는 영향
이선희,박용석,염혜원,송견지,한상철,배인하,Lee, Sun-Hee,Park, Yong-Seog,Yeum, Hye-Won,Song, Gyun-Jee,Han, Sang-Chul,Bae, In-Ha 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.1
Objective : Heat shock protein family is related to protective mechanism of cells by environmental changes. This study was performed to evaluate the effect of cryopreservation on the heat shock protein 90 (Hsp90) expression in mouse ovarian tissue. Methods : Cryopreservation of mouse ovarian tissue was carried out by slow freezing method. The mRNA level of Hsp90 expression in both fresh and cryopreserved mouse ovarian tissue was analyzed by RT-PCR. The protein expression of Hsp90 was evaluated by Western blot analysis and immunohistochemistry. Results: The mRNA and protein of Hsp90 were expressed in both fresh and cryopreserved mouse ovarian tissue. The amount of Hsp90 mRNA was increased in cryopreserved ovarian tissue after 60 and 90 minutes after thawing and incubation. The amount of Hsp90 protein was increased in the cryopreserved ovarian tissue after 6 hours of the incubation in Western blot analysis. In immunohistochemical study, Hsp90 protein was localized in cytoplasm of oocytes and granulosa cells. Significant level of immunoreactive Hsp90 protein was detected in theca cells contrast to the weak expression in ovarian epithelial cells. Conclusion: This results showed the increase of Hsp90 expression in both mRNA and protein level in the cryopreserved mouse ovarian tissue. It can be suggested that Hsp90 may play a role in the protective or recovery mechanism against the cell damage during cryopreservaion.
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균형 전좌 또는 Robertsonian 전좌 보인자의 체외수정 및 배아이식술에서 형광직접보합법을 이용한 착상전 유전자진단의 임상적 적용
전진현(Jin Hyun Jun),송견지(Gyun Jee Song),김정욱(Jeong Wook Kim),박소연(So Yeon Park),김계현(Kye Hyun Kim),최범채(Bum Chae Choi),궁미경(Mi Kyoung Koong),강인수(Inn Soo Kang),임천규(Chun Kyu Lim),한미현(Mi Hyun Han) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7
목적 : 본 연구는 균형 전좌 또는 Robertsonian 전좌 보인자의 체외수정 및 배아이식술에서 형광직접보합법을 이용한 착상전 유전자진단을 시행하여 그 결과와 효율을 알아보고자 시행하였다. 연구방법 : 본원에서 착상전 유전자진단을 시행한 15쌍, 25주기의 체외수정 및 배아이식술을 연구대상으로 하였으며, 3주기에서 54개의 극체를 이용하여, 22주기에서 234개의 할구를 이용하여 형광직접보합법으로 염색체의 숫적 이상을 분석하였다. 형광직접보합법을 이용하여 분석한 후 정상의 형광직접보합법 signal을 나타내는 배아만을 모체에 이식하였다. 임신이 확인된 경우 양수천자의 방법으로 태아의 핵형을 확인하였다. 결과 : 극체를 이용한 형광직접보합법 분석에서, 18개의 극체가 정상이었고, 3주기 모두에서 배아를 이식하였으며, 형광직접보합법의 효율은 95.0%였다. 할구를 이용한 형광직접보합법 분석에서는 49개의 배아가 정상으로 확인되었다. 정상의 배아를 확인할 수 없었던 1주기를 제외한 21주기에서 배아이식을 시행하였으며, 형광직접보합법의 효율은 92.7%였다. 3주기에서 임신이 되었고, 2주기에서 건강한 균형 전좌 보인자인 남아와 Robertsonian 전좌 보인자인 여아가 태어났다. 1주기는 임신이 진행중이며, 양수천자에서 정상의 핵형으로 확인되었다. 결론 : 형광직접보합법을 이용한 착상전 유전자진단은 균형 전좌 또는 Robertsonian 전좌 보인자의 체외수정 및 배아이식술에 성공적으로 적용되어 태아의 염색체 이상으로 인하여 발생되는 여러가지 문제를 해결할 수 있는 효과적인 방법으로 사료된다. Objective : This study was performed to evaluate the efficiency of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization (FISH) in Robertsonian or balanced reciprocal translocation carriers in human IVF-ET programm.Method : FISH was carried out in 25 cycles of 15 couples. Two-color FISH analysis was performed on 54 polar bodies in 3 cycles and 234 blastomeres in 22 cycles. After FISH analysis, the embryos with normal FISH signals were transferred into mother's uterus.Results : In FISH analysis of polar bodies, 18 nuclei of polar bodies were normal and 12 embryos were transferred in 3 cycles. FISH efficiency per oocyte was 95.0% in cases using polar bodies. In FISH analysis of blastomeres, 49 embryos were normal and transferred in 21 cycles. FISH efficiency per embryo was 92.7% using blastomeres. At present, three pregnancies were achieved. A girl and a boy were delivered. Both of them were translocation carriers. The other conceptus showed normal karyotype.Conclusion : According to this study, PGD using FISH can be successfully applied for the patients with translocations of chromosomes.
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