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Cloning of a Rat μ(mu)Opioid Receptor Gene
설은영,김균언 한국유전학회 1995 Genes & Genomics Vol.17 No.4
A μ (mu) opioid receptor (μORc) chromosomal gene was cloned from a genomic library of rat liver using μ opioid receptor cDNA as a probe. Restriction mapping and Southern blot analysis with a probe corresponding to the 5′ untranslated and the portion of N-terminal region of μORc cDNA have confirmed that the clone contains at least 3 Kb of 5′ flanking region of the gene. Sequencing analysis also revealed a translation initiation codon ATG in the 500 bp DNA fragment out of this region. Currently, a series of 5′ deletion mutants are being prepared to localize regulatory elements of the gene in this region. Northern blot analysis demonstrated that μORc gene is expressed in the hypothalamus-derived GT1-1 cells but not in pituitary-derived GH₃ cells. Furthermore, cAMP was shown to have minor regulatory effect on the expression of the gene in proportion to the concentration and treated time.
설은영,윤권혁,이희용,조성준,박종일,황성주,조정원 한국고분자학회 2022 폴리머 Vol.46 No.2
Alzheimer’s disease is a progressive neurodegenerative disease associated with memory deficit and cognitive decline. Herein, a new formulation of donepezil-loaded microspheres of uniform particle size was investigated. Donepezil-loaded microspheres were successfully prepared using a cross-flow membrane emulsification technology. The size analysis showed that the average diameter of the microspheres was 58-83 μm, indicating a narrow size distribution with a span value of less than 0.71. Scanning electron microscopy showed that the microspheres had a spherical shape. The loading efficiency of donepezil in the poly(D,L-lactic acid) (PLA) microspheres was 61.8±3.7% (w/w). Donepezil-loaded microspheres prepared using PLA (DM-L3) presented a more sustained release efficacy than poly(lactic-co-glycolic acid) (PLGA) microspheres in an in vitro accelerated release test and single-dose subcutaneous pharmacokinetic test in rats. The concentration-time profile analyzed using the non-parametric superposition method showed that the DM-L3 microspheres could achieve a sustained release of the drug for 1 month
흰쥐 μ(mu)opioid Receptor 유전자의 클로닝 및 발현 조절
설은영,송석빈,김균언 한국유전학회 1998 Genes & Genomics Vol.20 No.3
Chromosomal gene encoding a μ(mu) opioid receptor (μORc) was cloned from a rat genomic library using μORc cDNA as a probe. Sequence analysis of the clone identified a transcription start site, four AP-1 sites, two Oct-1 sites and one CREBP1 site in the immediate 5' flanking region as well as a translation initiation codon ATG in exon I. However, canonical TATA box was not found in the rat μORc promoter region. Northern blot analysis demonstrated that μORc gene is expressed in the hypothalamus-derived GT1-1 cells and pituitary gonadotrope-derived αT3-1 cells but neither in pituitary lactotrope-derived GH₃ cells nor in pituitary thyrotrope-derived αTSH cells. In addition, expression of the gene was induced in the cells treated with cAMP for 24 hrs. A series of deletion mutants of the 5'-flanking region were subcloned into the luciferase reporter vector, and tested for their transcriptional activities by transfecting into αT3-1 cells. The construct containing up to -2967 bp region exhibited maximum transcriptional activity among the tested constructs, whereas the region below -1705 bp conferred significantly low levels of activity. Therefore, it is conceivable that the region between -2967 bp and -1705 bp is responsible for the basal expression of the μORc gene.
김명희,설은영,박태신,박형우 한국유전학회 2000 Genes & Genomics Vol.22 No.1
Humans, rats, mice, and other species exposed to hyperthermia exhibit a variety of developmental defects. Development of central nervous system is particularly sensitive to hyperthermia. It is known that hyperthermia induces cell death in embryos and affects gene and protein expression. Homeobox-containing genes (Hox) are a family of regulatory genes encoding transcription factors that primarily play a crucial role during development and direct anteriorposterior axial patterning during embryogenesis. To observe morphological effects and gene expression pattern by heat exposure, day 9 p.c. (post-coitum) mouse embryos were exposed to hyperthermia in culture. Embryos exposed to 43℃ for 15 minutes induced significant decrease in crown-rump length and somite number, and delayed in ear and limb formation. The expression of Hoxa-7 gene in heat-treated embryos did not show any restricted pattern of expression along the anterior-posterior axis, whereas the control group have a distinct anterior boundary of expression, i.e., C5 in the ectoderm-derived spinal ganglia and neural tube, and T3-T5 in the mesoderm-derived prevertebrae. This result indicates that a brief heat shock at specific stage during embryogenesis can interfere with the normal establishment of Hox codes, and subsequently perturb the morphogenesis.
Chitin과 Chitosan이 흰쥐의 Cadmium중독과 지방대사에 미치는 영향
김미경,설은영 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-
This study was performed to investigate the effect of dietary chitin & chitosan on cadmium(Cd) toxicity and lipid metabolism in rats. Forty-two male rats of Sprague-Dawley strain weighing 137+-g were blocked into 6 groups according to body weight, and were raised for 4weeks. Cadmium chloride was given at the level of 0 or 400ppm in diet and chitin and chitosan wre given at the level of 0 or 4% (w/w) of diet. The results are summarzed as follow. Chitosan decreased the toxicity of Cd on liver, kidney and femur and increase the Cd content of fecal excretion. Chitosan increased the lipid & cholesterol content of fecal excretion by combining with lipid and bile acid. Chitosan decreased lipid, cholesterol and TG content in serum and liver by combining with lipid and bile acid. Chitin was less effective than chitosan in decreasing of Cd toxicity and lipid content of rat.