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택사의 Alisol B, Alisol B acetate 함량과 안정성
백미은,이영종,원재희 한국생약학회 2017 생약학회지 Vol.48 No.4
The current definition of Arismatis Rhizoma is the tuberous root of Alisma orientale Juzepzuk in the Korean Pharmacopoeia, but there is still no marker compound. So it has difficulties in quality control. Therefore, in this study, we have established a method to analyze alisol B and alisol B acetate using HPLC as a marker compounds of Arismatis Rhizoma. As the result of the analysis, alisol B content was ranged from 0.02% to 0.50% and alisol B acetate content was ranged from 0.12% to 0.25% in 40 samples. The stability of alisol B and alisol B acetate was investigated during 24 months. As a result, alisol B acetate was significantly decreased. The marker compound of Arismatis Rhizoma is alisol B acetate and the content of 0.05% or more is suitable by applying the stability results.
백미은,조덕조,정남혁,최민지,김정옥,원재희,이상한,권중호 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.10
To reduce microbial loads in medicinal herbs, Cnidii Rhizoma and Alismatis Rhizoma were subjected to electron-beam (e-beam) irradiation at doses (≤10 kGy) as permitted by the Korean Food Code. The effects of e-beam irradiation on the microbial load, stability of the active components, and anti-inflammatory activity of medicinal herbs were determined. We observed that the total aerobic bacteria (TAB; 4.0–7.0 log CFU/g), yeasts and molds (Y&M; 3.3–6.8 log CFU/g), and coliform counts (CC; 3.2–3.8 log CFU/g) in both herb samples were effectively reduced in a dose-dependent manner, resulting in acceptable levels of <3.0 log CFU/g in TAB and Y&M and negative in CC at 10 kGy irradiation. The concentration of the active components (0.87–4.22 mg/g) of Cnidii Rhizoma, including z-ligustilide, chlorogenic acid, senkyunolide A, and ferulic acid, in order of prevalence and those (0.86–2.76 mg/g) of Alismatis Rhizoma, including Alisol B acetate and Alisol B, were not changed at irradiation doses of ≤10 kGy. The extracts of e-beam irradiated Cnidii Rhizoma and Alismatis Rhizoma showed a reduced production of inflammation-related factors, such as nitric oxide, prostaglandin E2, interleukin (IL)-1β, and IL-6, in a concentration-dependent manner, which was induced by lipopolysaccharide in RAW 264.7 cell. However, there was no significant difference observed at e-beam irradiation doses of 0, 1, 5, and 10 kGy. Thus, we confirm that e-beam irradiation up to 10 kGy was effective for the control of microbial load in Cnidii Rhizoma and Alismatis Rhizoma without causing considerable changes in their major active components and anti-inflammatory activity. The results show the potential of e-beam application for sanitization of medicinal herbs.
백미은,Kashif Ameer,Yunhee Jo,정남혁,최민지,김정옥,원재희,이상한,권중호 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.5
Medicinal herbs comprise of heavy microbialcontaminations. This study aimed to assess microbialhazards including foodborne pathogens in 20 commercialmedicinal herbs, Cnidii Rhizoma (C1–C10) and AlismatisRhizoma (T1–T10) as well as to evaluate irradiation effectsof E-beam on microbial load and detection chracteristics. Four samples (C5, C10, T1, T8) from both herbs withhigher microbial load were selected for evaluating theirradiation effect of E-beam (up to 10 kGy) on microbialload and radiation-induced changes in detection markers bystandard methods (Codex, Korean Food Code), such asdirect epifluorescent filter technique/aerobic plate count(DEFT/APC), photostimulated luminescence (PSL), thermoluminescence(TL), and electron spin resonance (ESR). DEFT/APC revealed non-evidence of pre-sterilization ofall samples. PSL differentiated irradiated samples (1, 5, and10 kGy) of both herbs from non-irradiated (control:0 kGy). Both TL and ESR methods validated PSLscreening results by detecting radiation-induced markersfrom E-beam irradiated medicinal herbs.
백미은(Mi Eun Baek),성기운(Gi Un Seong),이영종(Young Jong Lee),원재희(Jae Hee Won) 대한약학회 2016 약학회지 Vol.60 No.5
The current definition of Cnidium Rhizome is the rhizome or the rhizome which is blanched by hot water of Cnidium officinale Makino or Ligusticum chuanxiong Hort. in the Korean Pharmacopoeia. However, the marker compound of Cnidium Rhizome is not specified, there are difficulties for quality control. Therefore, in this study we established the simultaneous determination method that analyze 4 different compounds by the HPLC-PDA. These compounds comprise chlorogenic acid, ferulic acid, senkyunolide A and z-ligustilide. As the result of the analysis, z-ligustilide has been shown highest content followed by senkyunolide A, ferulic acid and chlorogenic acid. These results can be exploited for a basic marker compounds of Cnidium Rhizome and for the legislation on the regulation and control of herbal medicines.