환삼덩굴 화분증 : 천식유발시험상 확진된 환자들의 임상적 특징

박해심,최소연,남동호,김희연 (Hae Sim Park,So Yeon Coi,Dong Ho Nahm,Hee Yeon Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.1

Baekground: Hop Japanese (Hop J) pollens are abundant in the air of Korea during the autumn season. Their significances as a source of allergenic sensitization have been underestimated in this country. Msterial and Method; In other to observe clinical features of Hop J-sensitive asthmatic patients in this country, skin prick test with Hop J pollen was performed. The serum specific IgE antibodies to Hop J pollen antigen were detected by enzyme linked immunosorbent assay (ELISA) in positive responders (>2+ of A/H ratio) on skin prick test. To confirm the respiratory sensitization, bronchoprovocation test was performed in 17 asthmatic patients sensi- tive to this pollen. Result: Ten asthmatic subjects showed a significant bronchoconstriction following the inhalation of Hop J pollen extract (6 early and 4 dual astmatic responses) and all of them had high serum specific IgE bindings, with minimal bindings in negative responders. They have suffered from seasonal aggravation of asthmatic symptoms with or without rhinitis, and/or conjunctivitis symptoms. The skin reactivity to Hop J had more than 5+ of A/H ratio on skin prick test in nine positive responders, whlie negative responders showed from 1+ to 3+ response. Moreover, four (40%) asthmatic subjects showed a positive response to only the Hop J pollen on skin prick test and an isolated positive asthmatic response to the Hop J bronchoprovocation test. Conelusion'. We believe that the Hop J pollen should be considered as an allergen during the Autumn season, and thus included in skin test batteries in this area. Some labelled having intrinsic asthma or rhinitis might be sensitized to this pollen or other unknown allergens.

Toluene diisocyanate 에 의한 직업성천식 환자의 객담내 호중구 활성화의 증거

김희연,남동호,박해심 ( Hee Yeon Kim,Dong Ho Nahm,Hae Sim Park ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Background: Toluene diisocyanate (TDI) is the most prevalent agent to cause occupational asthma (OA) in Korea. The pathogenic mechanism of TDI-induced OA is still unclear. Involvement of both immunological and non-immunologicaI mechanisms have been suggested. Objeetive '. To evaluate a possible role of neutrophil in the development of TDI-asthma. Object and method: Myeloperoxidase (MPO) as a neutrophil activation marker in both serum and induced sputum, and IL-8 in induced sputum were measured. Induced sputa and sera were collected from 15 TDI-induced OA patients (classified to group I) during TDI- bronchoprovocation test and were compared with those from 11 asthmatic subjects with negative TDI-bronchoprovocation test (group II). MPO levels were measured by radioimmunoassay, IL-8 levels, by enzyme linked immunosorbent assay and albumin levels, by nephelometry. Sputum MPO and IL-8 levels were presented as a ratio to albumin. Result: Serum MPO level tended to decrease during the TDI-bronchoprovocation test in two groups, but no statistical significance was reached (p>0.05). However, the ratios of MPO (the ratio of MPO level measured at 30 min to MPO level at baseline, and the ratio MPO level measured at 360 min to MPO baseline) in group I were significantly lower than group II (p=0.004, p=0.03 respectively). The IL-8/albumin and MPO/albumin levels in induced sputum from group I were significantly increased after the TDI-bronchprovocation test in comparison to the baseline value which was obtained before the bronchoprovocation test (p=0.0l, p=0.02 respectively). There was a significant correlation between the percent increase of IL-8/albumin and the MPO/albumin in induced sputum (r=0.89, p<0.05). Conclarsiog '. These findings suggest a possible involvement of neutrophil in the development of bronchoconstiction after the TDI exposure, and IL-8 might contribute to neutrophil recruitment to airway mucosa. Further investigation will be needed to investigate mechanism of neutrophil activation in the pathogenesis af TDI-induced OA.

산약 절편 씨마 종서의 저장온도 구명 및 화학적 방제 처리 효과

양예슬(Ye-Seul Yang),박해일(Hae Il Park),윤재연(Jae Yeon Yoon),양진희(Jin Hee Yang),프라바카란(Prabakaran mayakrishnan),오진영(Jin Young Oh),김승현(Seung Hyun Kim),임정대(Jung Dae Lim),안영섭(Yeong Sup Ahn),정일민(Ill-Min Chung) 한국약용작물학회 2014 한국약용작물학회 학술대회논문집 Vol.2014 No.2

기관지천식 환자의 기관지분비물내 총 IgE 항체치 연구에서 유도객담검사의 유용성

김상돈,서창희,김희연,남동호,박해심 (Sang Don Kim,Chang Hee Suh,Hee Yeon Kim,Dong Ho Nahm,Hae Sim Park) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.2

Background: IgE antibodies have been considered to play an important role in the pathogenesis of atapic asthma. However, there have been only few studies on the role of IgE in airway secretion in the pathogenesis of bronchial asthma. This might be partly due to difficulty in sampling of airway seceretion from asthmatic patients. Recently, sputum induction method by inhalation of nebulized hypertonic saline was developed, and proved to be valid and useful method for obtaining airway secretion from asthmatic patients for studying air- way inflammation. %jective and method: To evaluate the usefulness of sputum induction method for studying IgE antibodies in airway secretion from atopic asthmatic patients, total IgE levels in induced sputum from 54 atopic asthmatics were measured by enzyme-linked immunosorbent assay(ELISA) and tried to find an association with sputum eosinophilia. Result: Total IgE levels in induced sputum were significantly higher in atopic asthmatic patients(1.27+82.066 IU/ml) than in controls(0.203+0.291 IUgmP)(p<0.05). In atopic asthmatic patients, total IgE levels in induced sputum were not significantly different between patients with and without sputum eosinophilia(>5% of 200 counted leukocytes). There was a significant correlation of total IgE levels between induced sputum and serum in atopic asthmatic patients(r=0.60, p<0.05). Total IgE levels in induced sputum(1.278+ 2.066) were significantly higher than saliva sample(0.504 + 1.111 IU/ml) from atopic asthmatic patients(p<0.05). Conclusion '. These results suggest that total IgE levels are increased in the induced sputum of atopic asthmat,ic patients and sputum induction method is a useful tool for study- ing IgE antibodies in airway secretion from asthmatic patients.

비용 조직에서의 집먼지진드기에 대한 IgE 항체 검출

강지원,남동호,서경식,김희연,박해심 ( Ji Won Kang,Dong Ho Nahm,Kyung Sik Suh,Hee Yeon Kim,Hae Sim Park ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Objective '. In order to confirm the local production of total and specific IgE antibodies in the nasal polyp tissues. Material and method: We measured total IgE and house dust mite(Dermatophagoides pteronpssinus .' DP)-specific IgE antibody using enzyme-linked immunosorbent assay(ELISA) in the supernatant of nasal polyp homogenates from 72 subjects undergoing nasal polypectomy. The subjects were divided into three groups according to skin reactivity to DP: 20 strongly atopic subjects to group I(mean wheal diameter) 3mm), 19 weakly atopic subjects to group II (mean wheal diameter 1-3mm) and 33 negative skin responders to group III. Result: Group I showed significantly higher levels of total and DP-specific IgE levels in the nasa

의료인에서 발생한 췌장 추출물 흡입에 의한 직업성 천식

이수진(Soo Jin Lee),김희연(Hee Yeon Kim),서유진(Yu Jin Suh),김선신(Sun Sin Kim),이수걸(Soo Keol Lee),남동호(Dong Ho Nahm),박해심(Hae Sim Park) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.2

N/A Background and objective : Porcine pancreatic extracts (PPE) are composed of a-amylase, lipase, and protease which are common components of digestives. PPE are known to cause occupational asthma in pharmaceutical workers, but there has been no report of PPE-induced occupational asthma in medical personnel. The aims of this study were to document the rate of sensitization to PPE and the prevalence of PPE-induced occupational asthma in nurses exposed to PPE and to identify possible immunological pathogenic mechanisms. Subjects and method: A clinical questionnaire on bronchial asthma and allergic rhinitis was distributed among 56 nurses working in the medical ward of Ajou University Hospital. Then, allergy skin prick tests were done with PPE and a battery of eight common inhalant allergens. The determination of specific IgE antibodies to PPE, α-amylase, and lipase was performed by ELISA. The nurses with respiratory symptoms and positive skin tests to PPE underwent methacholine inhalation test and bronchial provocation test with PPE. Results. Of the 56 nurses, five (8.9%) had symptoms suggestive of bronchial asthma, and six (10.7%) had nasal symptoms suggestive of allergic rhinitis. Of these nurses, four (7.1%) had positive results on bronchial provocation test with PPE and proved to have PPE-induced occupational asthma. The prevalences of specific IgE to PPE and a-amylase were significantly higher in patients with positive skin tests (p<0.05). The prevalence of specific IgE to lipase was not significantly different between skin test positive and negative groups (p) 0.05). The prevalences of specific IgE to PPE and a-amylase were significantly higher in patients with respiratory symptoms(p<0.05), and four subjects with PPE-induced asthma had high specific IgE antibody to a-amylase, while specific IgE to lipase values were not significantly different between patients with respiratory symptoms and those without symptoms (p>0.05). Conclusion : These results support the view that inhalation of a-amylase within PPE powder may induce occupational asthma by IgE mediated reaction in exposed medical personnel.

제주도 농촌지역 주민의 벌독 알레르기 유병율

김윤근,장윤석,정재원,이병재,김희연,손지웅,이상록,조상헌,박해심,이명현,민경업,김유영 ( Yoon Keon Kim,Yoon Suk Jang,Jae Won Jung,Byung Jae Lee,Hee Yeon Kim,Jee Wong Son,Sang Rok Lee,Sang Heon Cho,Hae Sim Park,Myung Hyun Lee,Kyung Up Min,Y 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Backpwnd: Prevalence of bee venom allergy in Korea is unknown, Objective'. We performed a cross-sectional study to evaluate the prevalence of bee venom allergy and importance of risk factors, such as age, sex, and atopy, in the development of the allergic reaction in children and adults living in rural area of Cheju Island. Swbject and method: The prevalence was investigated in subjects with 1,054 children and 370 adults (701 male, 723 female), aged 7-87 years, living in rural area of Cheju Island. A history particularly focused on the systemic reactions after the bee stings was taken in all subjects. Skin prick test,s to common inhalant allergens, including house dust mites, fungi, tree, grass, mugwort and ragweed pollen, cockroach, animal epithelium, Japanese cedar pollen, and citrus red mite were performed. Result: The prevalence of bee venom allergy was 5.8% (mild systemic reactions 5.0%, severe systemic reactions 0.8% ). The prevalence was higher in adults than in children (10.2% vs. 5.0%, g = 12.0, p<0.01). Among t,he child!'en, the prevalerlce wB.S Signifcant,ly higher ill male than in female (8.6% vs. 1.3%, x=28.7, p<0.01), but no significant diffecence was noted between male and female in adults (10.0% vs. 10.3%, p>0.05). The prevalence of bee venom allergy was not significantly different between subjects with positive skin reaction and those with negative skin reaction to inhalant allergens (6.4% vs. 5.4% ), Conclasion'. The prevlence of bee venom allergy in inhabitants of rural area of Cheju Island is 5.8% (mild systemic,' 5.0%, severe systemic reaction. 0.8%), and bee venom allergy was more common in adults than in children, and in male children than in female children.

IgG1 및 IgG3 아형 결핍이 동반된 중증 아스피린 : 과민성 천식 환자에서 intravenous immunoglobulin 치료 1 례

박해심,김희연,한동석,남동호,안성균,유상용 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.4

Common complications in a patient with IgG subclass deficiency include recurrent respiratory infections and concomitant inflammatory lung disease. The most effective therapy in these patients is the administration of intravenous immunoglobulin. The authors report a case of severe aspirin-sensitive asthma and recurrent pneumonia with combined IgG1, and IgG3 subclass deficiency in a 19-year-old man. The patient was treated with 0.4g/kg at monthly intervals for 6 months, and is still receiving 0.2g/kg every 2 weeks at our clinic. After the replacement of in- travenous immunoglobulin, the patient has clinically improved.

아스피린 과민성 천식 환자에서 아스피린 기관지유발시험 전후의 혈청 호중구 화학주성능과 myeloperoxidase치의 변화

박해심,김희연,남동호,강경령,정기석,김선신 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.2

Background: There have been few reports suggesting involvement of mast cell and neutrophil to induce bronchoconstriction in aspirin-sensitive asthrna. Objective:To evaluate mast cell and neutrophil activation in pathogenesis of aspirin-sensitive asthma. Material and methods: We observed changes of serum NCA and MPO levels during L-ASA bronchoprovocation test in 14 subjects with aspirin-sensitive asthma. Results: Serum NCA was significantly increased at 30 min(p=0.01) after the inhalation of L-ASA and then, no significant changes were noted at 240 min (p=0.14). NCA was significantly higher in subjects with late asthmatic responses than in those without it (p=0.04). Serum MPO level tended to increase at 30 min with no statistical significance (p=0.08), and then it significantly decreased at 240 min (p=0.05). There was no significant correlation between serum NCA and MPO level (r=0.22, p=0.58). Conclusion: These results support the view that NCA derived from mast cell may contribute to neutrophil recruitment into the airway in aspirin-sensitive asthmatic patients.

후천성으로 추정되는 C1 esterase inhibitor 결핍이 동반된 혈관 부종 1례

박해심,김헌종,김희연,한동석,남동호,조재웅,정혁준,하창영,김선신 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.2

Angioedema is a well-demarcated localized edema involving the deeper layers of the skin, including the subcutaneous tissue. Angioedema occurs with Cl esterase inhibitor (Cl INH) deficiency that may be inborn as an autosomal dominant characteristic or may be acquired. Acquired angioedema (AAE) is a rare disorder characterized by adult onset and lack of evidence of inheritance of the disease. Two types of AAE are known today: type I in which there are lowering of functional Cl INH, an underlying disease such as a B-cell disease, and no detectable autoantibodies to Cl INH, type II with anti Cl INH autoantibodies in the circulation without detectable underlying disease and with depressed functional Cl INH levels. We experienced a case of angioedema in a 29-year old man. He had no family history of angioedema and laboratory data showed depressed Cl-INH levels. We diagnosed the case as acquired type of angioedema. Even though we could not measure anti-Cl INH auto-antibodies, we identified the case as type II because there was no evidence of underlying disease.