콩으로부터 상처 유도 beta-amyrin synthase 유전자의 동정 및 발현분석

박성환,이재헌,Park, Seong-Whan,Lee, Jai-Heon 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2

Suppression subtractive hybridization (SSH)를 통해 상처에 의해 발현이 유도되는 cDNA들을 분리하였고, 그 중 하나인 gmwi33은 $\beta$-amyrin synthase 유전자들과 높은 유사성을 보였다. gmwi33의 전장 cDNA인 GmAMS1은 2416 bp 길이에 739개 아미노산으로 구성된 긴 open reading frame(ORF)를 포함하고 있었다. GmAMS1 단백질은 감초의 $\beta$-amyrin synthase인 GgbAS와 89%, 완두의 OSCPSY와 86%의 유사성을 보였다. 암조건 하에 5일간 기른 콩나물에서, GmAMS1는 빛을 쪼여주었을 때 가장 강하게 발현되었고 methyl jasmonate 처리와 저온처리 시에도 발현이 유도된 반면, UV-B나 elicitor를 처리하였을 때는 발현이 유도되지 않았다. 이러한 GmAMS1의 발현양상은 사포닌의 활성산소 제거기능과 밀접한 연관이 있을 것으로 추측된다. Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of wound-induced cDNA, gmwi33 showed high homology with genes encoding $\beta$-amyrin synthase. The full length cDNA of gmwi33, designated GmAMS1, is 2416 bp long and contains an open reading frame consisted of 739 amino acids. GmAMS1 protein showed 89% identity with licorice GgbAS1 and 86% identity with pea OSCPSY. In 5 day-old, dark-grown seedlings, the expression of GmAMS1 was most strongly induced by light and weakly induced by methyl jasmonate and by low temperature. However, GmAMS1 was not induced by elicitor or UV-B treatment. Such expression pattern might be closely related with the oxygen-radical scavenging activity of soyasaponin.

Stable Expression of Yeast Acid Phosphatase in Transgenic Nicotiana Tabacum Plants

박성환,박영구,조진기,정원일 ( Seong Whan Park,Young Goo Park,Jin Ki Jo,Won Il Chung ) 한국유전학회 1994 Genes & Genomics Vol.16 No.2

Pho5 gene of Saccharomyces cerevisiae encodes a repressible acid phosphatase regulated by Pi level in a medium. For introduction of pho5 gene into plant genome and subsequent expression, the 1.4kb pho5 open reading frame was fused down to CaMV (Cauliflower mosaic virus) 35S promoter in pBSW1. It was introduced into tobacco plants by Agrobacterium-mediated leaf disc transformation method. The introduced pho5 gene is highly expressed in tobacco tissues under the control of CaMV35S promoter, as revealed by Northern blot analysis. Both the roots and leaves of the F1 progenies also showed high levels of pho5 expression. Furthermore, the increased phosphatase activity was measured in the leaf tissues, suggesting that the active form of PHO5 protein is synthesized in the transgenic tobacco.

협업설계를 위한 엔지니어링 프레임워크 개발에 관한 연구 -자동차 서스펜션 모듈에의 적용-

박성환,이재경,이한민,Park, Seong-Whan,Lee, Jai-Kyung,Lee, Han-Min 한국전산구조공학회 2008 한국전산구조공학회논문집 Vol.21 No.6

This paper describes an e-Engineering framework to support collaborative design of automotive suspension systems developed at KIMM(Korea Institute of Machinery and Materials). The e-Engineering framework is proposed and developed on the base of the multi-layered software agents including engineering task agent which is generated from the domain knowledge of experts. The developed framework is aim to widely spread application to the small and medium enterprises by adopting open source technologies such as JADE (Java Agent Development Framework) and by using the independent characteristics related with applicant H/W and 81W system. This framework can provide an integrated design environment to support distributed personnel, design activities and engineering resources during product development process. For the validation of the system's applicability and efficiency, the several practical design processes for automotive suspension systems of RR/FR lower arms and RR cross member are applied and discussed.

저온 스트레스에 발현이 유도되는 콩의 L-asparaginase 유전자의 분리

박성환,김기영,진량,이재헌,Park, Seong-Whan,Kim, Kee-Young,Chen, Liang,Lee, Jai-Heon 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2

Suppression subtractive hybridization (SSH)를 통해 저온에 의해 발현이 유도되는 cDNA들을 분리하였고, 그 중 하나인 slti182는 asparaginase 유전자들과 높은 유사성을 보였다. Slti182의 전체 cDNA인 GmASP1은 1258 bp 길이에 326개 아미노산으로 구성된 긴 Open reading frame (ORF)를 포함하고 있었다. GmASP1 단백질은 애기장대의 putative asparaginase (AB012247)와 84%의 유사성을 보였으나, 애기장대의 다른 isoform(Z34884)와는 55%의 유사성을 나타내었다. GmASP1 유전자는 저온 처리 후 3시간째부터 발현이 유도되어 6시간째에 최대발현을 나타내었고, 이후 점차 감소하여 48시간에는 저온처리전 수준으로 되돌아왔다. 또한, 식물체를 저온에서 48시간 둔 후에 다시 상온으로 옮겼을 때, 정상수준으로 떨어진 GmASP1의 발현이 다시 증가하는 양상을 보였다. 이러한 결과로 볼 때, GmASP1이 저온 스트레스에 반응 초기의 단백질 합성 촉진에 중요한 역할을 할 것으로 사료된다. Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.

부식과 도장을 고려한 선체잔여수명예측시스템 설계

박성환(Seong-Whan Park),이한민(Han Min Lee) 대한조선학회 2013 대한조선학회 논문집 Vol.50 No.2

In this paper, the design procedure and results for Residual Life Prediction System Considering Corrosion and Coating are explained, which is one module of Life-cycle Management System of Ship and Offshore Plants Operation. This Residual Life Prediction System has two main functions; one is residual life prediction function based on probability processing using corrosion measurement data of ships major structural members, and another is rust rate prediction function based on visual image processing of inspection photos. The analysis of system user requirements and functions are introduced, and the structure and environment of the developed system are explained.

도시형자기부상열차 시스템엔지니어링 관리계획

박성환(Seong-Whan Park),김동성(Dong-Sung Kim),백수현(Su-Hyun Back),정진철(Jin-Cheol Jung),조흥제(Hung-Je Cho) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

This study describes the SEMP(Systems Engineering Management Plan) being applied for the successful promotion of a national project of 'Urban Maglev Program'. SEMP means a kind of fundamental document for total integrated managements of the project and of the systems engineering activities. Since the tasks and responsibilities of all participants of the project, involving the tasks to collaborate between them, can be clearly defined and described in the SEMP, all participants can share and clarify their role and responsibility. The national project of 'Urban Maglev Program' are composed of three core projects; the first one for system integration, the second for the development of Maglev vehicle, and the third for a model line construction.

폰툰식 VLFS의 초기구조설계에 관한 연구

박성환(Seong-Whan Park),이탁기(Tak-Kee Lee),홍사영(Sa Young Hong) 대한조선학회 2005 대한조선학회 논문집 Vol.42 No.6

도시형자기부상열차 시스템엔지니어링 기술적용

박성환(Park Seong-Whan),김동성(Kim Dong-Sung),백수현(Back Su-Hyun),정진철(Jung Jin-Cheol),조흥제(Cho Hung-Je) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.11월

The general of systems engineering technology, as an effective supporting tool for the large complex system development, is introduced on the focus that the SEMP(Systems Engineering Management Plan) being applied for a national project of 'Urban Maglev Program'. SEMP means a kind of fundamental document for total integrated managements of the project and of the systems engineering activities. Since the tasks and responsibilities of all participants of the project, involving the tasks to collaborate between them, can be clearly defined and described in the SEMP, all participants can share and clarify their role and responsibility. The national project of 'Urban Maglev Program' are composed of three core projects; the first one for system integration, the second for the development of Maglev vehicle, and the third for a model line construction.

PCR 기법을 이용한 간편한 DNA Size Marker 제작 방법

박성환(Seong-Whan Park) 산업기술교육훈련학회 2021 산업기술연구논문지 (JITR) Vol.26 No.1

DNA size markers are widely used in molecular biology laboratories as DNA molecular weight standards. In this paper, I report a simple and convenient method for the preparation of DNA ladders using PCR technique. DNA ladders usually range from 100 bp to 10 kb, and many such 100 bp and 1 kb DNA ladders are commercially available. Conventionally, 100 bp to 5 kb DNA fragments were amplified using standard PCR with Taq DNA polymerase, and the large DNA fragments exceeding 5 kb were amplified using long-range PCR with a special thermostable polymerase. In this study, several types of DNA ladders were prepared by mixing the amplified DNA fragments in different combinations, which can be used as DNA size standards in agarose gel electrophoresis.

아가로스 겔 전기영동에서 간단하고 실용적인 DNA 염색 방법

박성환(Seong-Whan Park) 한국산업기술융합학회(구. 산업기술교육훈련학회) 2022 산업기술연구논문지 (JITR) Vol.27 No.4

핵산 염색시약은 아가로스 겔 전기영동에서 DNA 밴드를 관찰하기 위해 필요하다. 전통적인 염색법은 겔 전기영동이 끝난 후 염색하는 post-staining 방법이지만, 편리한 대안으로 겔에 염색시약을 첨가하는 pre-cast 염색법이 사용되어 왔다. 또 다른 염색 방법으로 염색시약을 아가로스 겔이 아닌 6X 로딩 버퍼에 첨가하는 pre-load 염색법이 있다. Pre-load 염색법은 가장 간단하고 시간을 절약할 수 있는 방법이지만, 동일한 겔 내에서 DNA 밴드의 이동성이 차이를 보일 가능성이 있다. 본 연구에서는 염색시약이 혼합된 Midori Green Direct, GRGreen DNA loading buffer, ENVISION DNA dye loading buffer 등 상용의 loading buffer를 사용할 때, DNA 이동성에 변화가 나타나는 것을확인하였다. 반면에 250X GelRed®를 함유한 자체제작 6X 로딩 버퍼는 다양한 실험 조건에서도 이동성의 변화를나타내지 않았다. 마찬가지로, Shinystar와 EcodyeTM가 포함된 6X 로딩 버퍼 또한 안정적인 이동성을 보여주었다. 결론적으로, pre-load 염색법은 일상적인 겔 전기영동 작업에서 편리하고 실용적인 염색방법이 될 수 있으며, 250X GelRed®를 함유한 자체제작 6X 로딩 버퍼는 pre-load 염색에 있어 유용한 해결책이 될 수 있다. Nucleic acid staining dyes are necessary for the visualization of DNA bands in agarose gel electrophoresis. The conventional staining method follows a post-staining protocol that follows gel electrophoresis; pre-cast staining is used as a convenient alternative. Pre-load staining is another method in which a staining dye is added to the 6X loading buffer, and not in the agarose gel. Pre-load staining is simple and time-saving; however, it could alter the mobility of DNA bands within the same gel. In this study, the mobility alteration owing to the use of commercial pre-mixed loading buffers, such as Midori Green Direct, GRGreen DNA loading buffer, and ENVISION DNA dye loading buffer, were evaluated. Lab-prepared 6X loading buffer containing 250X GelRed®dye did not alter the mobility under various experimental conditions. Similarly, 6X loading buffer containing Shinystar and EcodyeTMdid not affect mobility. In conclusion, pre-load staining method is a convenient and practical staining method for routine gel electrophoresis; lab-made 6X loading buffer containing 250X GelRed®could be used for pre-load staining.