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Ascorbic acid, citric acid 및 활성탄이 가시 없는 음나무 조직배양시 캘러스에 미치는 영향
박기덕,김세원,최병곤,문윤기,서현택,권순배 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Kalopanax pictus is one of the most popular wild vegetables in Korea. In recent years Kalopanax pictus has been developed which has no thorns that can be efficiently harvested. Kalopanax pictus, now without thorns, breeds only by root cutting. This makes the price of ineffective and invisible Kalopanax pictus high. Therefore, tissue culture is necessary for efficient mass proliferation of visibly absent Kalopanax pictus. This study was conducted to investigate the effect of anti-browning treatment on tissue culture of thornless Kalopanax pictus. Methods and Results : Thornless Kalopanax pictus young leaves were cut into 5 ㎜ in diameter and cultured for 1 month at 2,4-D 1 ppm + TDZ 0.1 ppm + 3% sucrose. Add 0.05%, 0.1%, 0.15% and 0.2 ㎎/ℓ of browning inhibitor such as ascorbic acid, citric acid and activated charcoal to the MS + 2,4-D 0.2 ppm + 5% sucrose medium. And cultured at 25℃ for 24 hours Light condition for 1 month. In order to determine the optimum concentration of browning agent, characteristics such as callus size, adventitious shoot formation rate and browning degree were investigated. As a result, the callus size of ascorbic acid 0.05 ㎎/ℓ was the largest at 2.26 ± 0.4 ㎝ and the adventitious shoot formation rate was the highest at 16.4%. The degree of browning was 40.30 ± 4.92 in L value and 5.00 ± 2.23 in b value. Conclusion : When ascorbic acid 0.05 ㎎/ℓ is added to the secondary culture medium, the size of the callus increases and the degree of browning decreases. Using the above-mentioned results, we can establish a mass proliferation system through tissue culture of Thornless Kalopanax pictus.
교외 주거지역의 사회적 자본 영향요인과 주거단지 활성화에 관한 연구
박기덕,하성규 한국지역개발학회 2011 한국지역개발학회 세미나 논문집 Vol.2011 No.1
Although therehavebeen many previousstudiesthatshow that,socialcapital has made much more impacts on the improvementofquality oflife and the creation of wealth than does physicalcapital.This study is to revealinfluencing factors in social capitalin suburban area,namely apartmentestates in Kwacheon city .In following two majorquestionsareaddressed in thispaper:(1)which Influencing factorsofsocialcapital existin suburban area? (2)whatkind ofdifferences existin terms ofperception of the level of social capital and thereputation of the areain differenthousehold characteristics? Using theresult of126questionnaires,we access commonly recognized element of inclusive community which issocialcapital.Wesuggestthatinsuburban areawhilehousing tenure wasnotrelevantto thedevelopmentofneighborhood-based socialcapitaland socialmix, housing duration wasthecrucialfactorofsocialcapital,particularly trustand creation of norm andthatthesefactorneedtobeconsideredbysocialplanners,housingpolicymakers and other sinvolved in the implementation of human settlements policies.
박기덕,박용범,Hee Seung Nam,Tai Kon Kim,Seong Hoon Kang,Min Ho Lim 대한재활의학회 2012 Annals of Rehabilitation Medicine Vol.36 No.1
Objective To investigate the short-term effects and advantages of sono-guided capsular distension, compared with fl uoroscopically guided capsular distension in adhesive capsulitis of shoulder. Method In this prospective, randomized, and controlled trial, 23 patients (group A) were given an intra-articular injection of a mixture of 0.5% lidocaine (9 ml), contrast dye (10 ml), and triamcinolone (20 mg); they received the injection once every 2 weeks, for a total of 6 weeks, under sono-guidance. Twenty-five patients (group B)were treated similarly, under fl uoroscopic guidance. Instructions for the self-exercise program were given to all subjects, without physiotherapy and medication. Eff ects were then assessed using a visual numeric scale (VNS),and the shoulder pain and disability index (SPADI), as well as a range of shoulder motion examinations which took place at the beginning of the study and 2 and 6 weeks after the last injection. Incremental cost-eff ective ratio (ICER), eff ectiveness, preference, and procedure duration were evaluated 6 weeks post-injection. Results The VNS, SPADI, and shoulder motion range improved 2 weeks after the last injection and continued to improve until 6 weeks, in both groups. However, no statistical differences in changes of VNS, SPADI, ROM, and eff ectiveness were found between these groups. Patients preferred sono-guided capsular distension to fl uoroscopically guided capsular distension due to diff erences in radiation hazards and positional convenience. Procedure time was shorter for sono-guided capsular distension than for fl uoroscopically guided capsular distension. Conclusion Sono-guided capsular distension has comparable effects with fluoroscopically guided capsular distension for treatment of adhesive capsulitis of the shoulder. Sono-guided capsular distension can be substituted for fl uoroscopic capsular distension and can be advantageous from the viewpoint of radiation hazard mitigation,time, cost-eff ectiveness and convenience.