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Bromoxynil 특이성 Nitrilase 유전자를 이용한 제초제 저항성 형질 전환 식물의 분자육종
민복기(Bok Kee Min),박은성(Eun Sung Park),박연홍(Yearn Hung Park),송재영(Jae Young Song),이세영(Se Yong Lee) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.4
Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic, plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.
피부 표면 온도의 변화와 diffusion chamber내의 온도 변화에 따른 경표피수분손실 및 피부 장벽 회복률의 변동
민복기 ( Pok Kee Min ),김도원 ( Do Won Kim ),전재복 ( Jae Bok Jun ),정상립 ( Sang Lip Chung ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.6
Background: The epidermal permeability barrier resides in the intercellular spaces of the stratum corneum and is composed of lipids. Objective : The purpose of this study was to measure the changes of transepidermal water loss (TEWL) and the recovery rate of the epidermal permeability barrier according to the skin surface temperature(SST) using an evaporimeter after tape-stripping in vivo and after treatment with several kinds of solvent in vitro. Methods : In the in vivo study, basal TEWL and SST were measured on both flanks of the neonatal rats consisting of an!.sthetized and non-anesthetized groups. The epidermal barrier was disrupted by repeated tape-strippings which were terminated when the TEWL reached 20-50 gram/m/h. TEWL and SST were measured immediately and 2.5, 6, 10, 24, 48 and 72 hours after tape-stripping. For the in vitro study, sheets of epidermis were separated from the circumcised prepuce, and were pretreated with acetone, petroleum ether or distilled water for 6 minutes. A piece of the separated epid~ermis was placed in a diffusion chamber, and TEWL was measured with an evaporimet,er that was placed onto the top of the chamber over a temperature range of 25 37C in the chamber. Results : In the in vivo study, SST increased according to the elevation of the ambient temperature in neonatal rats with a statistical significance(p<0.05). The recovery rates of both non anesthetized and anesthetized groups increased in the higher SST especially during the first 10 hours. We observed the effect of the temperature in the diffusion chamber on TEWL after treatment with several kinds of solvent in vitro. The data obtained was plotted semi-logarithmically with TEWL as a function of temperature in the chamber, but there was no a statistical difference among the solvent treated grorps. Conclusion. According to the results of the study, SST should be clearly rnentioned in any kind of studies concerning TEWL measurement. Further studies of the relationships between TEWL and SST are needed. ~(Kor J Derrnatol 1996;34(6): 875-885)
Paenibacillus sp. JB-13 Cyclodextrin Glucanotransferase 유전자의 E. coli 에서의 발현 및 최적 생산
김해윤,이상현,김해남,민복기,백형석,전홍기,Kim, Hae-Yun,Lee, Sang-Hyeon,Kim, Hae-Nam,Min, Bok-Kee,Baik, Hyung-Suk,Jun, Hong-Ki 한국미생물학회 2008 미생물학회지 Vol.44 No.1
L-ascorbic acid (AA)의 2번 위치의 수산기에 부위 특이적 활성을 갖는 Paenibacillus sp. JB-13 유래의 cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) 유전자(cgt gene)를 pEXP7 발현 vector에 클로닝하여 재조합 균주를 구축하였다. 재조합 균주의 CGTase생산 최적 조건을 검토하여 본 결과 LB 배지에 0.5% glucose, 3.0% polypeptone, 0.3% $K_2HPO_4$, 0.5% NaCl이 되도록 추가하고, 초기 pH 7.0, 접종량 2%, 통기량 0.1 vvm, 배양 온도 $37^{\circ}C$, 배양 시간 14시간의 조건에서 최대 활성을 나타내었다. 재조합 균주와 기존 균주의 CGTase 활성을 비교한 결과 재조합 균주는 배양 14시간째 640 units/ml의 활성을 가져 기존 균주에 비해 70%의 활성을 나타내지만 배양 시간을 1/4로 단축시킬 수 있다는 이점이 있음을 확인하였다. 재조합 균주가 생산한 CGTase를AA-2G합성에 적용하여 AA-2G를 합성하고 HPLC로 분석한 결과 단일 peak를 확인할 수 있었고 ${\alpha}$-glucosidase를 처리하여 확인한 결과 AA와 glucose로 분리됨을 확인할 수 있었다. The purpose of this study is to clone cgt gene from Paenibacillus sp. JB-13 and to overexpress the protein in E. coli. For this purpose, the cgt gene was amplified from Paenibacillus sp. JB-13 genomic DNA by PCR using degenerate oligonucleotide primers. The sequence analysis results showed that the cgt gene from Paenibacillus sp. JB-13 has 98% homology with the cgt gene of Bacillus sp. To overexpress the protein, the cgt gene was cloned into pEXP7 expression vector and transformed into E. coli. The production of CGTase by recombinant E. coli was optimized under following conditions: 0.5% glucose, 3.0% polypeptone, 0.3% $K_2HPO_4$, 0.5% NaCl, and 7.0 of initial pH, 2.0% of inoculum, $37^{\circ}C$ of culture temperature for 14 hr. And the optimal agitation was found at 0.1 vvm. The synthesis of 2-O-${\alpha}$-D-Glucopyranosyl L-Ascorbic acid (AA-2G) using the CGTase expressed in E. coli was identified as AA-2G by HPLC and HPLC confirmed that treating AA-2G made by cloned CGTase with ${\alpha}$-glucosidase substantially produced AA and glucose.
소각로 중온 폐열 재활용 위한 복식형 열전발전시스템 개발
박수동(Park, Su-Dong),김봉서(Kim, Bong-Sea),오민욱(Oh, Min-Wook),민복기(Min, Bok-Kee),이희웅(Lee, Hee-Woong) 한국신재생에너지학회 2009 한국신재생에너지학회 학술대회논문집 Vol.2009 No.06
소각로를 포함한 다양한 산업설비의 배폐열은 열병합 등의 다양한 방법을 통해 재활용되고 있으나 에너지의 효율적 사용과 편의성을 고려할 때, 단순한 온수공급 등의 방법보다는 전력으로서의 재활용이 매우 필요하다. 특히 재활용이 어려운 400?C이내의 중저온급 폐열원을 발전할 수 있는 유력한 방안으로 열전발전기술이 최근 부각되고 있다. 열전발전은 발전모듈의 변환효율이 7~10%이고, 시스템 효율은 5%내외로 증기발전에 비해서는 낮지만 기계적 가동부분이 없어 고장발생이 적고 기동정지가 용이하며 열이 있으면 바로 발전이 가능한 차세대 친환경 발전기술이다. 본 연구에서는 현재까지 시도, 개발되지 못한 100?C에서 400?C내외 온도영역인 중저온급 소각폐열 회수를 위한 목적으로 중온용 열전발전소재 및 모듈과 저온과 중온에 각기 대응하여 폐열발전의 효용성을 높인 복식열전발전시스템을 개발 중에 있다. 본 고에서는 현재까지 진행된 일부 연구내용들을 소개하고자 하였다.
안병근(Ahn Byoung Keun),이건주(Rhee Gun Joo),민복기(Min Bok Kee),한호진(Hahn Ho Jin),정기영(Chung Ki Young) 대한구강악안면외과학회 1988 대한구강악안면외과학회지 Vol.14 No.1
This is a retrospective study on 516 patients with maxillofacial bone fracture. The patients were treated at HAN GANG SANCRED HEART HOSPITAL from Jan.1,1977 to Dec. 31, 1986. The results were as follows. 1) The male to female ratio was about 4.6:1. 2) The facial bone fractures occured most frequently in the twenties (38.9%). 3) The most frequent etiologic factor was traffic accident. 4) A symphysis area was the most traumatic site of mandible. 5) A zygomatic areas were the most traumtic sites of facial middle 1/3. 6) In case of maxillofacial bone fracture with other side injuries it is most frequently accompanied by head injuries.
염증이 있는 치아의 발거 전후의 혈액학적 변화에 관한 연구
민복기,김경욱 단국대학교 치의학연구소 1989 논문집 Vol.1 No.-
The purpose of this study was to evaluate the hematologic changes following extraction of teeth with periodontal inflammation. The subjects were adult males between the ages 20 and 50. Blood specimens were obtained 1 hour prior to extraction and 3 hours, 6 hours, 12 hours and 24 hours after extraction. Each sample was evaluated for W. B. C.count with differential. R. B. C. count, hemoglobin, hematocrit and erythrocyte sedimentation rate. The results were as follows: 1. R. B. C. count. Hemoglobin & Hematocrit value showed no remarkable changes after extraction. 2. E. S. R. count increased remarkably after extraction. 3. W. B. C. count increased after extraction. 4. percentage of neutrophil increased & percentage of lymphocyte decreased slightly after extraction.
전상환자에서 구순 구각부 결손에 대한 재건의 치험 4례
최규환,강명수,민복기,정철우 大韓顎顔面成形再建外科學會 1993 Maxillofacial Plastic Reconstructive Surgery Vol.15 No.3
A technical refinement for oral commissure reconstruction is presented. The oral commissure has an important role in oral sphincteric function. Once lost, the oral commissure is very difficult to restore and therefore, reconstruction of the oral commissure requires prevention of this function as well as prevention of microstomia. Trauma and tumor excisions are the most frequent reasons that will necessitate reconstruction of the oral commissure. Direct approximation of the wound margins after resection of the corrner of the mouth could easily induce microstmia and difficulty in opening. Thus case presented here deals with distortion of noraml mouth angle and acquired microstomia due to burn and tissue deface following shotgun and explosive wounds. The a mucosal flap approach was used to rehabilitate a natural looking mouth angle showing satisfactory results function and esthetic wise.