담수토양중 부식물질에 대한 diazinon 의 흡착

송재영,이규승 ( Jae Young Song,Kyu Seung Lee ) 한국환경농학회 1993 한국환경농학회지 Vol.12 No.1

In order to find the effect of humic substances affecting to the behavior of diazinon in submerged soil, the adsorption rate of diazinon was investigated with different soil humic substances like as humin, humic acid and fulvic acid. The adsorption rate of diazinon(1.8 ppm) was 12.4% in humin, 11.9% in fulvic acid and 10.4% in humic acid at 1% concentration of humic substances, also were not much differences at 0.1 and 0.5%. But it showed much similar level (10.2∼10.6%) at 1.0% concentration in 5ppm diazinon treatment. As a result, because adsorption rate of diazinon on humic substances were about 10-12%, disappearance of diazinon in submerged soil may be affected by the other factors such as soil microorganism.

AZCA 저항성 돌연변이 세포주로부터 선발 육성만 내염성 벼 돌연변이 계통의 특성 검정

송재영,김동섭,이긍주,이인석,강권규,윤성중,강시용,Song, Jae-Young,Kim, Dong-Sub,Lee, Geung-Joo,Lee, In-Sok,Kang, Kwon-Kyoo,Yun, Song-Joong,Kang, Si-Yong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1

본 연구는 벼 배 배양 캘러스에 방사선 조사와 AZCA 처리를 통해 AZCA저항성 세포주를 선발 육성하고 proline 함량이 증가된 선발 계통을 중심으로 염분 스트레스에 저항성을 갖는 벼 계통을 육성하고 그 기작을 밝히고자 하였다. 먼저, 1) AZCA 저항성 후대로부터 NaCl 저항성 식물체를 선발하고, 2) 선발된 저항성 계통의 생리적 생화학적 특성을 분석하였으며, 3) 분자적 특성을 RT-PCR을 통해 조사하고 유전적 변이를 탐색하였다. AZCA저항성 $M_{3}$ 3,000 계통으로부터 얻어진 약 20,000 종자에 염분 적정 선발 농도로 밝혀진 1.5%의 염분을 처리하여 내염성 (ST) 벼 116 개체를 선발하고, $M_{4}$ 후대 세대를 양성하였다. $ST\;M_{4}$ 세대에서 2차 내염성 계통 선발을 위해서 $M_{4}$ 세대계통을 1.2% NACl 에서 대조구와 생육 조사한 결과, 대조구 식물은 생육이 약하고 성장이 지연되는 것을 볼 수 있었다. 내염성 계통(ST-13, ST-16)으로부터 유도된 캘러스에 NaCl 처리한 결과, 대조구, ST-13, ST-16의 생존율은 9%, 16%, 20%로 나타났다. 또한, 필수 아미노산 함량을 잎, 종자 및 캘러스로 나누어 분석한 결과 ST-13와 ST-16는 대조구와 비교하여, 1) 잎에서는 약 1.24, 1.3배, 2) 종자에서는 1.49, 2.43배, 3) 캘러스에서는 1.32, 1.60배 증가하는 것이 확인되었다. 내염성 계통과 대조구에서 이온함량을 비교한 결과 잎과 뿌리에서 $K^{+},\;Na^{+}$ 및 $Na^{+}/K^{+}$ 비율을 보면 대조구보다 $Na^{+}/K^{+}$ 비율이 낮아진 것이 확인되었다. 내염성과 연관된 유전자 P5CS, NHXI를 이용하여 RT-PCR 실험을 수행한 결과, 돌연변이 계통에서 이들 유전자의 발현이 증가됨을 확인할 수 있었다. 본 연구에서 선발된 계통은 내염성 육종 및 기초 연구를 위한 재료로 이용될 수 있을 것으로 사료된다. To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.

논토양미생물의 Diazinon 과 부식물질에 대한 기질 친화성

송재영,이규승 ( Jae Young Song,Kyu Seung Lee ) 한국환경농학회 1992 한국환경농학회지 Vol.11 No.2

In order to investigate waether soil microorganism prefer diazinon to humic substances as their substate, the growth of soil bacteria and the activities of monooxygenase and α-,β-esterase were measured after treatment of diazinon and humic substances in defined medium at 30±1℃. Also, the degradation rate of diazinon was determined by addition of humic substances to the medium. The number of soil bacteria was increased from 1 day and 3 days after the treatment with humic substances and diazinon, respectively. And it showed about 1.5 times more with humic substances than diazinon at 10 days. Monooxygenase(MO) activity with the treatment of humic substances was higher than diazinon until 3 days after treatment in the order of HA > FA > humin. Esterase(ES) activity with the treament of humin and HA was higher than dizainon from 5 dyas, but FA was much similar to diazinon. The degradation rate of diazinon showed more persistancy by addition of humic substances ; 51.4% with humin treatment, 58.9% with HA, 62.4% with FA and 71.9% in control at 10 days after treatment. Therefore, as soil microorganisms perfer humic substances to diazinon, the degreadation rate of diazinon might be delayed by addition of humic stbstances in submerged soil.

임베디드 시스템을 위한 가상 파일 시스템 구현

송재영(Jae-Young Song),이호송(Ho-Song Lee),성영락(Yeong Rak Seong),이철훈(Cheol-Hoon Lee),권택근(Taeck-Geun Kwon) 한국정보과학회 2003 한국정보과학회 학술발표논문집 Vol.30 No.1A

임베디드 시스템에 대한 관심과 개발이 급속도로 진행됨에 따라 임베디드 시스템에서도 파일 시스템을 필요로 하게 되었다. 특히 실 시간 운영체제 (Real Time Operating system : RTOS)를 사용하는 경우에는 운영체제가 사용되는 임베디드 시스템에 따라 필요로 하는 파일 시스템이 하나 또는 그 이상으로 다르게 되어 그 파일시스템을 통합하여 관리하기 위한 가상 파일 시스템 (Virtual File system : VFS)을 필요로 하게 되었다. 본 논문에서는 실 시간 운영체제와 함께 동작을 하는 가상 파일 시스템을 구현하는 방법을 제시한다.

수술후 발생한 장피누공의 임상 고찰

송재영(Jae Young Song),유완식(Wan Sik Yu),서정욱(Jung Wook Suh) 대한외과학회 1985 Annals of Surgical Treatment and Research Vol.28 No.2

Purification and Properties of Thermostable L - Iactate Dehydrogenase Produced by Escherichia coli

송재영(Jae-Young Song),김경숙(Kyoug-Sook Kim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

Bacillus stearothermophilus의 유산탈수소 효소에 대한 4.3kb인 유전자를 subcloning하여 대장균에서 생산된 효소의 특성을 조사하였다. 본 효소는 25%의 수율로 200배 정제되었으며, 열처리, OEAE-Sephadex A-50, NAD^+ -Sepharose CL-4B affinity chromatography, gel filteration을 거쳐서 정제되었다. Gel filteration에 의해 정제된 효소의 분자량은 약 140,000이었으며, SDS-polyacrylamide 전기영동 결과, 분자량 35,000의 단일 band를 나타내어 4개의 subunit로 구성된 효소임을 알 수 있었다. 정제효소에 대한 pyruvate 환원 반응과 lactate 산화 반응에 있어서의 최적 pH는 각각 5.5와 8.5이었고, pH 안정성은 pyruvate 환원 반응에 있어서 5에서 7 사이였다. 효소활성에 대한 최적온도 및 열안정성은 pyruvate 환원 반응에서 60℃, lactate 산화 반응에서 70℃와 75℃이었다. 본 효소는 FeSO4와 pCMB에 의해 강한 저해 반응을 나타내었으며, sodium oxalate는 경쟁적 저해제로 작용하였다. 또한 fructose 1,6-bisphosphate에 의한 효소활성 촉진은 0.8mM에서 최대의 활성을 나타내었다. The 4.3-kb gene coding for L-Iactate dehydrogenase of Bacillus stearothermophilus has been subcloned and expressed in E. coli cells. The enzyme was purified 200-fold with 25% yield by heat treatment, DEAE-Sephadex, and NAD^+ -Sepharose CL-4B affinity chromatography followed by gel filtration through Sephadex G-200. The molecular weight of the purified enzyme was estimated to be about 35,000 and 140,000 on SDS-polyacrylamide gel electrophoresis and gel filtration, respectively, indicating that the enzyme is composed of four identical subunits. The enzyme for pyruvate reduction and lactate oxidation was stable at 60 and 75℃ for 30min, and the optimal temperatures for both reactions were 60 and 70℃, respectively. The enzyme had an optimal pH at 5.5 and 8.5 in pyruvate reduction and lactate oxidation, respectively. The pH stability of enzyme for pyruvate reduction was stable between pH 5 and 7. More than 90% of enzyme activity was lost at 1mM FeSO₄ and p-chloromercuribonzoate. The maximal activation of the enzyme was obtained with 0.8mM fructose 1,6-bisphosphate.

제대기저부 탈장

송재영(Jae Young Song),김상윤(Sang Youn Kim),서정욱(Jung Wook Suh) 대한외과학회 1985 Annals of Surgical Treatment and Research Vol.28 No.1

한국 재래식 된장 발효중 관여 미생물이 생성하는 향기 성분

송재영(Jae Young Song),안철우(Cheol Woo Ahn),김종규(Jong Kyu Kim) 한국미생물생명공학회 1984 한국미생물·생명공학회지 Vol.12 No.2

『시민의 자치력』에 의한 대의제의 극복방안 연구

송재영 ( Song Jae-young ),이경훈 ( Lee Kyung-hoon ) 극동대학교 사회복지연구소 2020 글로벌사회복지연구 Vol.10 No.1

This article starts with the worries about finding ways to supplement and overcome the shortcomings and problems of modern representative democracy. Therefore, rather than representative politics by representatives elected through elections, it is intended to comprehensively define various democratic ways in which the sovereign people become the subject of politics as autonomous democracy, and seek ways to supplement and overcome the representative through case studies. In order to study alternatives, I studied four cases that are being realized by the direct governance of the people. In real politics, autonomous democracy does not conflict with the representative system, and it can be confirmed that all autonomous democracies work together. Therefore, through the analysis of autonomous democracy that operates in various forms, I proposed ways to strengthen the representative system that does not properly reflect the sovereignty of the people and to deepen democracy.

DEA 모형을 이용한 세계 주요 항만의 효율성 평가

송재영(Jae-Young Song),신창훈(Chang-Hoon Sin) 한국항해항만학회 2005 한국항해항만학회지 Vol.29 No.3

본 연구는 부산항을 포함한 세계 주요 컨테이너 항만들의 효율성을 DEA(Data Envelopment Analysis)모형을 통해 상대적으로 분석함으로써, 부산항의 현재 위치와 더 효율적인 항만이 되기 위해 벤치마킹(Benchmarking) 해야 할 대상을 구체화 하였다. 또한, 본 연구에서는 일정시점의 효율성 분석이 아닌 1995년~2001년까지 7개년의 효율성을 시계열적으로 분석함으로써 각 항만의 효율성 변화를 살펴보았다. 분석 결과, 부산항은 95년, 96년을 제외한 나머지 연도에 대해서 비효율적인 항만으로 구분되었으며, 97년 이래로 효율성 지수가 다소 증가하고 있는 것으로 나타났다. This paper presents the measurement of efficiency for container ports. Data envelopment analysis(DEA), as it is called, has particular applicability in the service sector. Applying mathematical programming techniques, DEA enables relative efficiency ratings to be derived within a set of analysed units. This paper investigates the efficiency employing DEA Model on data for 53 container ports covering 1995~2001 in the world and the change in efficiency for 7 years. As a results, port of Busan was evaluated as inefficiency port compare with major ports of the world except 1995year and 1996year. But After 1997year, efficiency of Busan port is increasing somewhat better every year.