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생쥐 배자의 cDNA 라이브러리로부터 신규 유전자(Jpk)의 동정
김혜남,박성도,박형우,김명희 한국유전학회 2002 Genes & Genomics Vol.24 No.2
During the study on the regulation of Hox gene expression, one novel cDNA (C171, Jopock Jpk) has been isolated, and the total nucleotide sequence (1148 bp) of cDNA has been determined. It encodes an 187 as-long ORF (open reading frame) containing one trans-membrane domain (AA_(22-51)) in it. Computer analysis revealed that a human homologue of C171 (Jpk) is a hypothetical uni-gene, AD-015 located on the locus, LOC113213 of human chromosome 15q26. When EGFP (enhanced green fluorescent protein) fusion construct, EGFP-C171 (JPK) was generated and transfected into the F9 teratocarcinoma cells in an attempt to trace the localization of the JPK protein inside of the cell, most of the fluorescent cells died except small portion: viable cells expressed the fusion protein in the cytoplasm, particularly in the granular structure. The viability of the transfected cell was reduced dramatically in a time dependent manner; less than 50% of the cells survived after 72 hours transfection. The transfection of Jpk induced DNA fragmentation, generating nucleosomal ladder and the sub-G1 fraction of DNA in the nucleus, and increased the exposure rate of phosphatidyl serine on the cell surface. In order to identify the sequence necessary for trans-activation of JPK, several reporter constructs containing different regions of PSRE (position specific regulatory element) were generated. Cotransfection analysis revealed that the construct NS218 containing two GAGA binding sites was the most responsible fragment. The results altogether indicate that the expression of JPK should be regulated precisely during development since a trace amount of the protein is very toxic, leading the host cell to death.
蜂毒藥鍼刺戟이 腦幹 神經細胞와 Serotonin性 神經細胞의 活性變化에 미치는 영향
金惠南,高炯均,朴東錫,姜成吉,金容奭,崔容泰 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-
This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of serotonergic(5-HT) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats, the number of Fos immunoreactive neurons was counted by using computerized image analyzing system. Also, the number of colocalization between 5-HT containing neurons Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1.In almost every neucli, the Chok-Samni group and Blank locus group showed more increase in the number of Fos immunoreactive neurons than the control group. Especially, in Arc, DR, LC, RMg, Gi, PAG Rost and PAG LV, the Chok-Samni group showed more significant increase than the control group. Also, in PAG LV Mid and Arc, Chock-Samni group showed more significant increase than the Blank locus group. 2.In DR and PAG LV Mid, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between 5-HT containing neurons and Fos immunoreactive neurons than the control group after the BV aqua- acupuncture. Also, the Chok-Samni group showed more significant increase than the Blank locus group. Consequently, the BV aqua-aqupuncture increased more potent the number of Fos immunoreactive neurons and the activity of serotonergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as serotonin Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.
김혜남 한국무용동작심리치료학회 2000 한국무용동작심리치료학회논문집 Vol.1 No.1
정신분석학은 문학이론에서와는 달리 영화이론에서는 늦게 채용되었다. 영화이론은 1910년 무렵부터 존재해 왔지만 대체로 형식적이고 작가중심적이며 사회학적인 고려가 주류를 이루었다. 문학이론은 1930년대와 1940년대에 정신분석학을 채용했으나 영화이론에서 정신분석학은 1970년대 초에야 등장하였다. 영화가 Freud의 정신분석학과 동시대의 산물이었다는 점을 감안하면(둘 다 19세기 후반에 태동했고 둘의 발전사에는 공통점이 많다), 이것은 다소 기이하게 여겨진다. 더구나 영화가 판타지 및 꿈과 쉽사리 연결된다는 사실도 이러한 지연에 대해 우리를 의아하게 만든다.
봉독약침자극(蜂毒藥鍼刺戟)이 뇌간(腦幹) 신경세포(神經細胞)와 Serotonin성(性) 신경세포(神經細胞)의 활성변화(活性變化)에 미치는 영향
김혜남,고형균,박동석,강성길,김용석,최용태,Kim, Hye-Nam,Koh, Hyung-Kyun,Park, Dong-Suk,Kang, Sung-Keel,Kim, Yong-Suk,Choi, Yong-Tae 대한침구의학회 2000 대한침구의학회지 Vol.17 No.2
This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of serotonergic(5-HT) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats, the number of Fos immunoreactive neurons was counted by using computerized image analyzing system. Also, the number of colocalization between 5-HT containing neurons Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1. In almost every neucli, the Chok-Samni group and Blank locus group showed more increase in the number of Fos immunoreactive neurons than the control group. Especially, in Arc, DR, LC, RMg, Gi, PAG Rost and PAG LV, the Chok-Samni group showed more significant increase than the control group. Also, in PAG LV Mid and Arc, Chok-Samni group showed more significant increase than the Blank locus group. 2. In DR and PAG LV Mid, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between 5-HT containing neurons and Fos immunoreactive neurons than the control group after the BV aqua- acupuncture. Also, the Chok-Samni group showed more significant increase than the Blank locus group. Consequently, the BV aqua-acupuncture increased more potent the number of Fos immunoreactive neurons and the activity of serotonergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as serotonin Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.