족관절 해면골의 미세 구조 분석

김현정,안태선,백명현,최문권,원예연,Kim, Hyon-Jeong,An, Tae-Sun,Baek, Myong-Hyun,Choi, Mun-Kwon,Won, Ye-Yeon 대한족부족관절학회 2004 대한족부족관절학회지 Vol.8 No.2

Purpose: This study aimed to investigate and compare the micro-structural profiles of trabecular bone from different facets at the ankle joint. Materials and Methods: In a fresh cadaver ankle, four cored 10 mm of diameter cylindrical specimens of trabecular bone were harvested from the distal tibia, the talar dome, the medial malleolus, and the lateral malleolus. Using a micro-computed tomography, two-dimensional and three-dimensional micro-structural indices of the trabecular bone were analyzed. Results: Each specimen from the tibia, talus, medial malleolus, and the lateral malleolus showed unique micro-structural pattern. Tibia versus talus, the talus was seen a higher bone volume fraction and a wider supporting zone subchondrally whereas the tibia was seen a relatively lower bone volume fraction and a much narrower supporting zone subchondrally. Lateral malleolus versus medial malleolus, the lateral malleolus was seen the thicker but sparse trabeculae pattern whereas the medial malleolus was seen the thinner but more compact trabecular pattern. Conclusion: Each four locations from the different facet at the ankle joint have distinct own micro-structural patterns of the trabecular bone, suggesting different mechanical properties.

Humic Acid Removal from Water by Iron-coated Sand: A Column Experiment

김현정(Hyon Chong Kim),Seong Jik Park,Chang Gu Lee,Yong Un Han,Jeong Ann Park,Song Bae Kim 대한환경공학회 2009 Environmental Engineering Research Vol.14 No.1

Column experiments were performed in this study to investigate humic acid adhesion to iron oxide-coated sand (ICS) under different experimental conditions including influent humic acid concentration, flow rate, solution pH, and ionic strength/composition. Breakthrough curves of humic acid were obtained by monitoring effluents, and then column capacity for humic acid adsorption (Ccap), total adsorption percent (R), and mass of humic acid adsorbed per unit mass of filter media (q(a)) were quantified from these curves. Results showed that humic acid adhesion was about seven times higher in ICS than in quartz sand at given experimental conditions. This indicates that humic acid removal can be enhanced through the surface charge modification of quartz sand with iron oxide coating. The adhesion of humic acid in ICS was influenced by influent humic acid concentration. Ccap and q(a) increased while R decreased with increasing influent humic acid concentration in ICS column. However, the influence of flow rate was not eminent in our experimental conditions. The humic acid adhesion was enhanced with increasing salt concentration of solution. Ccap, q(a) and R increased in ICS column with increasing salt concentration. On the adhesion of humic acid, the impact of CaCl₂ was greater than that of NaCl. Also, the humic acid adhesion to ICS decreased with increasing solution pH. Ccap, q(a) and R decreased with increasing solution pH. This study demonstrates that humic acid concentration, salt concentration/composition, and solution pH should be controlled carefully in order to improve the ICS column performance for humic acid removal from water.

골관절염 연골세포의 세포사열과 그 조절기전

김현정 ( Hyon Jeong Kim ),신의철 ( Eui Cheol Shin ),유남진 ( Nam Jin Yoo ),박전한 ( Jeon Han Park ),김성재 ( Sung Jae Kim ),강응식 ( Eung Shick Kang ),장준섭 ( Jun Seop Jahng ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.2

목적: 골관절염(osteoarthritis)은 관절연골의 점차적인 소실과 이에 따른 활액막과 연골하골의 이차적인 변화를 특징으로 하는 질환으로서 특히 노년층에서 높은 유병율을 보이는데 원인 및 병인에 대해서는 아직 뚜렷하게 규명된 바가 없다. 한편, 세포사멸(apoptosis)은 세포괴사(cell necrosis)와는 상대되는 개념의 세포 사망으로서 염증반응이 없이 진행되는 것이 특징이고 자가면역질환, 종양, 퇴행성 뇌질환 등의 기전연구에서 그 역할이 규명되고 있다. 최근 몇몇 연구에 의하면, 골관절염의 연골세포가 정상관절의 연골세포에 비해 높은 비율의 세포사멸을 보이며, 또한 Fas에 대한 항체를 처치한 경우에도 부분적으로 세포사멸을 일으킨다고 하였다. 본 연구는 골관절염의 연골세포에서 일어나는 세포사멸의 양상을 정상관절의 연골세포와 비교하고, 세포사멸과 관련한 수용체/리간드의 발현을 조사하여 골관절염의 병인에 있어서 세포사멸의 조절기전을 알아보았다. 방법: 골관절염으로 인공슬관절 치환술을 시행받은 환자의 관절연골과 외상에 의해 슬상절단술을 시행 받은 환자의 절단지에서 채취한 관절연골을 재료로, TUNEL assay를 통해 세포사멸의 정도와 위치를 비교하였고 관절연골 조직에서 RNA를 분리하여 역전사중합효소연쇄반응을통해 세포사멸의 조절에 참여하는 Fas 관련분자 및 bcl-2family, TRAIL 및 그 수용체와 인터루킨-18 (IL-18)의 mRNA 발현정도를 조사하였으며 면역조직화학염색을 통하여 FasL 단백의 발현을 알아보았다. 결과: 형광 dUTP를 이용한 TUNEL assay에서 골관절염이 없는 정상 대조군 관절연골 4례 모두에서는 세포사멸을 보이는 세포를 찾아볼 수 없었으나 골관절염의 관절연골 7례의 표본 중 6례에서 전형적인 세포사멸 세포들을 관찰할 수 있었다. 역전사중합효소연쇄반응에서는 caspase-3, caspase-8, Fas 및 FasL의 mRNA 발현정도가 골관절염의 연골에서 정상 대조군 연골에 비해 증가하였으며, 특히 FasL는 일부 골관절염에서 현저히 증가된 양상을 보였다. FasL의 유도체로 알려진 IL-18은 FasL가 증가된 환자에서 함께 강한 발현을 보였다. 면역조직화학염색에서는 정상 대조군 연골에서보다 골관절염의 연골에서 FasL 단백이 현저히 강하게 발현됨을 관찰하였다. 결론: 본 연구는 골관절염 환자의 관절연골에서 의미있게 증가한 세포사멸과 FasL의 발현을 관찰하였으며, 이는 Fas/FasL 경로를 통한 세포사멸이 골관절염에서 일어나는 관절연골의 퇴화에 기여할 것을 시사한다. 또한 FasL의 유도체 중 하나인 IL-18의 발현도 관찰되었다. 이상의 결과는 차후 골관절염 치료에 있어서 새로운 접근을 제시하는 데 일조할 것으로 기대된다.

족관절 외측 구획과 내측 구획의 시상면 형태의 차이

안태선,김현정,An, Tae-Sun,Kim, Hyon-Jeong 대한족부족관절학회 2004 대한족부족관절학회지 Vol.8 No.2

Purpose: Anatomic configuration of the ankle joint is useful information during various ankle procedures. This study was prospectively designed to investigate the sagittal inclination pattern of the ankle joint. Materials and Methods: Lateral standing ankle radiographs from one-hundred people (age: $17{\sim}68$, M:F=45:55) and magnetic resonance (MR) sagittal ankle images from twenty-four people (age: $16{\sim}65$, M:F=14:10) were studied. Post-traumatic, arthritic, or grossly deformed ankles at any reason were excluded. The posterior inclination angle (PIA) of the distal tibia was measured and compared between the lateral and the medial segment. Results: On plain radiographic data, the average PIA of the distal tibia was $6.14^{\circ}{\pm}3.56^{\circ}$ (range $0^{\circ}$ to $14^{\circ}$) in the lateral segment and $13.16^{\circ}{\pm}3.05^{\circ}$ (range $6^{\circ}$ to $22^{\circ}$) in the medial segment. On MR imaging data, the average PIA of the distal tibia was $5.08^{\circ}{\pm}4.26^{\circ}$ (range $1^{\circ}$ to $10^{\circ})$ in the lateral segment and $10.16^{\circ}{\pm}4.87^{\circ}$(range $5^{\circ}$ to $17^{\circ}$) in the medial segment. The PIA between two segments was significantly different. Conclusion: The sagittal configuration of the medial area ankle joint and the lateral area ankle joint has difference in the degree of posterior inclination and shape of curvature.

일절개법을 이용한 관절경적 후 십자 인대 복원술 - 이절개법과의 비교 -

김성재,김현곤,김현정,김한식,Kim, Sung-Jae,Kim, Hyun-Kon,Kim, Hyon-Jeong,Kim, Han-Sik 대한관절경학회 1998 대한관절경학회지 Vol.2 No.1

This study was done to compare the results of the one-incision technique and the conventional two incision technique for the arthroscopic treatment of the posterior cruciate ligament injury. Fifty-five patients with the posterior cruciate ligament injury underwent the arthroscopic posterior cruciate ligament reconstruction using bone-patellar tendon-bone(BTB) graft. Patients with combined ligament injuries requiring concomitant operative treatment were excluded in this study. The conventional two-incision technique was performed in ten patients(Group I) and the one-incision technique in forty-five patients(Group II). The average duration of follow-up was 45 months in Group I(range, 40 to 50 months) and 36 months in Group II(range, 24 to 68 months). Auto BTB grafts were utilized for all patients in Group I. In Group II, 34 BTB autografts and 11 BTB allografts were utilized. The functional results were evaluated according to the Lysholm Knee Scoring scale and the Hospital for Special Surgery(HSS) knee ligament rating form. The postoperative posterior laxity was measured with a KT 1000 or 2000 arthrometer. Lysholm postoperative mean values were 90.0 in Group I and 90.6 in Group II. HSS mean values were 87.7 in Group I and 92.6 in Group II. HSS postoperative mean value showed better results in Group II(p=0.037). The average side-to-side difference of the posterior translation measured by the KT 2000 arthrometer were 2.10 mm(range, 1 to 4 mm) in Group I and 2.38 mm(range, 0 to 5 mm) in Group II. But there was no statistically significant difference. In Group II, the results of the autograft and allograft showed no significant difference. The arthroscopic posterior cruciate ligament reconstruction using one-incision technique showed good results comparable to the conventional two-incision technique. This technique minimizes potential injury to the extensor mechanism, especially vastus medialis obliquus, and scar formation over the medial femoral condyle. The operation can be finished within one tourniquet time by using only one-incision.

3세 소년에서 발생한 비골 외과의 외상성 골결손후 자연재생

박희완(Hui-Wan Park),김현정(Hyon-Jeong Kim),박병문(Byeong-Mun Park) 대한정형외과학회 1996 대한정형외과학회지 Vol.31 No.5

양측 견관절의 선천성 이두근 장두건 결손증

김성재(Sung-Jae Kim),김현정(Hyon-Jeong Kim),박재현(Jai-Hyun Park) 대한정형외과학회 1998 대한정형외과학회지 Vol.33 No.2

Stainless 금속에 의한 슬관절내의 이물질 반응 및 활액막염

김성재(Sung Jae Kim),이윤태(Yun Tae Lee),김현정(Hyon Jeong Kim),강지호(Ji Ho Kang) 대한정형외과학회 1997 대한정형외과학회지 Vol.32 No.7

슬관절 골관절염 환자에서 Hyruan(R)(Sodium Hyaluronate)의 유효성 및 안정성 평가

이신석 ( Shin Seok Lee ),주영실 ( Yeong Shil Joo ),김완욱 ( Wan Uk Kim ),박성환 ( Sung Hwan Park ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ),김현정 ( Hyon Jeong Kim ),김성재 ( Sung Jae Kim ) 대한류마티스학회 1999 대한류마티스학회지 Vol.6 No.1

Objectives: To assess the clinical efficacy and safety of Hyruan(R)(sodium hyaluronate) in patients with osteoarthritis of the knee. Methods: A 12-week open study of Hyruan(R) was conducted in 48 patients with osteoarthritis(OA) of the knee. Hyruan(R) was injected into knee joints weekly for 5 weeks. Clinical evaluations were performed using visual analog scale(VAS) for pain, Lequesne`s index, tenderness and swollen joint counts weekly for the first 5 weeks and then monthly up to 3 months post-injection. Results: Significant improvements in VAS, Lequesne`s index, tenderness and swollen joint counts were noted from the first week and maintained until the end of 3 month follow-up period. At 3 months post-injection, 68.8% of patients had ≥20mm reduction in the VAS. The patients with Kellgren stage III showed significant response in VAS and Lequesne`s index as patients with Kellgren stage I and II. The clinical improvement after treatment was slower in patients(n=16) with late(≥5 years) OA than in patients(n=32) with early(<5 years) OA, but it was statistically significant in either groups, compared to baseline. There were no severe adverse reactions to Hyruan(R) therapy and laboratory examinations revealed no evidence of toxicity. Conclusion: Hyruan(R) has been shown to be effective and safe in the treatment of patients with osteoarthritis of the knee.

석영 및 철피복 모래에서 박테리아 부착·탈착

이창구(Chang Gu Lee),박성직(Seong Jik Park),김현정(Hyon Chong Kim),한용운(Yong Un Han),박정안(Jeong Ann Park),김성배(Song Bae Kim) 大韓環境工學會 2009 대한환경공학회지 Vol.31 No.4

본 연구에서는 칼럼실험을 이용하여 석영모래와 철피복 모래에서 박테리아(Escherichia coli, Bacillus subtilis, 그리고 Staphylococcus aureus)의 부착 및 탈착에 미치는 이온강도의 영향을 조사하였다. 실험결과, 철피복 모래에서는 이온강도가 1 mM에서 100 mM로 증가함에 따라 질량회수율이 일정(E. coli=13.7±0.5%, B. subtilis=9.8±1.3%, S. aureus=13.0±2.1%)한 반면, 석영모래에서는 80.7%에서 45.3%로 감소하였다(S. aureus). 용출용액의 이온강도가 100 mM에서 0.1 mM로 감소함에 따라, 석영모래에서는 평균 39.1%의 박테리아 탈착이 일어났지만, 철피복 모래에서는 탈착이 관찰되지 않았다. 철피복 모래에서 관찰된 이러한 현상은 박테리아와 철피복 모래사이의 inner-sphere complexes(이온강도의 영향을 받지 않는)에 의한 결합 때문이다. 본 연구는 박테리아와 표면변형 다공성 여재의 상호작용에 대한 지식을 증진시킨다. This study investigated the influence of ionic strength on the adhesion and release of bacteria (Escherichia coli, Bacillus subtilis, and Staphylococcus aureus) in quartz and iron-coated sands using column experiments. Results show that the mass recovery remained constant (E. coli=13.7±0.5%, B. subtilis=9.8±1.3%, S. aureus=13.0±2.1%) in iron-coated sand while it decreased from 80.7 to 45.3% (S. aureus) in quartz sand with increasing ionic concentrations from 1 to 100 mM. As the ionic concentrations of leaching solution was lowered from 100 to 0.1 mM, average 39.1% of bacterial detachment was quantified from quartz sand, but no bacterial release was observed in iron-coated sand. The phenomenon observed in iron-coated sand can be attributed to the inner-sphere complexes between bacteria and coated sand, which have minimal effect from ionic strength. This study improves our knowledge regarding the bacterial interaction with surface-modified porous media.