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온라인 고체상추출과 LC-MS/MS 기술을 이용한 소변 중 파라벤류 분석
김정환,고영림,김판기,정지연,이은희,이승열,남혜선,이규식,Kim, Jung Hoan,Kho, Young Lim,Kim, Pan Gyi,Jeong, Jee Yeon,Lee, Eun Hee,Lee, Seung-Youl,Nam, Hye-Seon,Rhee, Gyu-Seek 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.6
Objectives: Parabens are widely used as antimicrobial agents in pharmaceuticals and cosmetics as well as by the food industry. Parabens have been reported to show weak estrogenic activity and be related to health effects such as allergic reactions and skin and breast cancer. We evaluated an online solid phase extraction (SPE) method coupled with LC-MS/MS technique using free and conjugated parent parabens in human urine for assessing human exposure to parabens. Methods: We employed LC/MS/MS through online solid phase extraction and column-switching techniques and analyzed free and conjugated parabens as biomarkers of human exposure. Four major parabens, methyl-paraben (MP), ethyl-paraben (EP), propyl-paraben (PP) and butyl-paraben (BP), were analyzed. Method validation was performed by sensitivity, accuracy, precision and comparison of the results of online SPE with offline SPE. Results: The limits of detection (LOD) were in the range of 0.2-2 ng/mL, and actual limits of quantification (LOQ) were in the range of 0.7-6 ng/mL urine, depending upon the compound. Accuracy was in the range of 98.3-106.4%, and precision was in the range of 1.3-8.7% (CV) depending upon the compound. We found a good correlation between the results of analysis by online SPE method and that by off-line SPE method. Conclusions: The online SPE method showed proper LOD and validated accuracy, precision and good correlation with the offline method for analyzing parabens in urine.
김정환 ( Jung Hoan Kim ),이옥섭 ( Ok-sub Lee ),한지숙 ( Ji-sook Han ),신성연 ( Sung-yeon Shin ),백두현 ( Doo-hyun Baek ),하병조 ( Byung-jo Ha ) 대한화장품학회 2008 대한화장품학회지 Vol.34 No.4
본 연구에서는 모발의 내부 및 외부구조에 자외선이 미치는 영향에 관하여 조사를 수행하였다. 자외선이 조사된 모발의 형태학적 및 화학적 구조의 변화를 알아보기 위해 주사전자현미경(SEM), 투과전자현미경(TEM), 공초점레이져주사현미경(CLSM) 등을 사용하였다. SEM상에서 자외선이 조사된 모발은 자외선에 의한 화학적 산화반응에 의해 표면이 거칠고 부풀어진 형태를 보였다. 이황화결합(disulfide bond)의 산화에 의한 시스테인산(cysteic acid)의 분해로 팽윤부위가 관찰되었다. 또한 자외선 조사에 의해 표면색의 변화를 나타내었다. TEM 분석결과 버진헤어(virgin hair)의 경우에는 매끄러운 표면구조를 나타내었지만, 자외선 조사모발에 있어서는 거칠고 모수질층이 갈라진 형태를 보여주었다. 모피질층에 존재하는 멜라닌 입자의 크기가 감소하고 소실되는 것이 관찰 되었다. CLSM에 의해 3차원적 구조의 입체영상을 얻을 수 있었으며, 광학분할에 의해 버진헤어는 강한 형광을 나타내었지만, 자외선 조사모발은 낮은 형광을 보여주었다. 이는 버진헤어의 경우 자동형광을 나타낼 수 있는 방향족 아미노산이 많이 존재하는데 비해 자외선 조사모발은 방향족 아미노산이 파괴되어 낮게 나온 것으로 여겨진다. In this study, we have investigated the effects of ultraviolet (UV) irradiation on the external and internal structure of human hair. For changes in morphological and chemical structure of UV-exposed hair, we utilized several microscopic techniques such as scanning electron microscope (SEM), transmission electron microscope (TEM), confocal laser scanning microscope (CLSM), and etc. The SEM showed the scales of UV-exposed hair appeared to be rough and bulkier because of chemical oxidation during UV irradiation. Small bulgy area of UV-exposed hair surface was appeared as a result of the decomposition in cysteic acid. In the TEM picture, the UV-exposed hair showed the cleaved aperture of hair and destruction of melanin granules. Three dimensional topographical images were obtained by using CLSM. In the optical single section, high fluorescent intensity appeared in virgin hair. In the case of UV-exposed hair, low fluorescence intensity appeared. This means the aromatic amino acids in virgin hair were more abundant than UV-exposed hair.
Thyme(Thymus vulgaris L.) 추출물의 Helicobacter pylori 억제효과 및 생리활성
김정환 ( Jeung Hoan Kim ),권효정 ( Hyo Jung Kwon ),이경환 ( Kyeong Hwan Lee ),천성숙 ( Sung Sook Chun ),권오준 ( Oh Jun Kwon ),우희섭 ( Hi Seob Woo ),조영제 ( Young Je Cho ),차원섭 ( Won Seup Cha ) 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.3
캐모마일(Matricaria chamomilla L.)의 생리활성
조영제(So-Jung Yoon),윤소정(Jeung-Hoan Kim),김정환(Young-Je Cho),천성숙(Sung-Sook Chun) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.4
캐모마일(Matricaria chamomilla L.)을 성인병 예방을 위한 기능성 식품 소재로 이용하기 위하여 추출물의 생리활성 효과를 조사하였다. 추출물의 phenol 함량은 열수추출물이 23.64±0.35 ㎎/g이었으며, 60% 알콜추출물은 24.98±0.20㎎/g으로 60% 알콜추출물의 phenol 함량이 다소 높게 나타났다. 캐모마일의 HPLC 분석 결과 생리활성 효과가 높은 rosemarinic acid, quercetin의 함량이 많은 것으로 보아 생리활성 효과가 있을 것으로 사료된다. 각 추출물의 항산화 효과는 DPPH가 열수추출물과 60% 알콜추출물이 각각 91.05%와 95.49%로 높게 나타났고, antioxidant protection factor (PF)는 열수추출물이 0.71±0.02로 지용성 물질에 대한 항산화력은 낮았으나, 60% 알콜추출물은 1.48±0.03으로 높은 PF를 나타내어 열수추출물보다는 60% 알콜추출물이 지용성에 대한 항산화력이 더 높다는 것을 알 수 있었다. Helicobater pylori에 대한 추출물의 항균활성은 열추추출물의 경우 clear zone이 나타나지 않았으며, 60% 알콜추출물의 경우 200 ㎍/mL의 농도일 때 9.42 ㎜의 clear zone이 관찰되어 60% 알콜추출물에서만 낮은 저해활성이 있는 것으로 판단되었다. ACE 저해효과는 열수추출물이 87.98%, 60% 알콜추출물이 91.36%의 저해효과를 나타내어 높은 고혈압 효과를 나타내었다. XOase에 대한 억제효과는 열수추출물이 73.48%, 60% 알콜추출물이 81.96%의 저해효과를 나타내어 XOase에 대한 높은 저해를 관찰할 수 있었다. 이러한 결과로 캐모마일의 60% 알콜추출물이 항산화제, 고혈압 및 항관절염 소재로의 개발이 기대되어었다. The biological activity of water and ethanol extracts from Chamomaile for functional food source were examined. Total phenol contents in the 60% ethanol extracts (24.98±0.20 ㎎/g) from Chamomaile leaf was higher than those of water extracts (23.64±0.35 ㎎/g). The major phenolic compound by HPLC were rosemarinic acid and quercetin. 60% ethanol extracts had higher content of these phenolics than water extacts. Electron donating ability showed 91.05% in the water extracts and 95.49% in the 60% ethanol extracts. Antioxidant protection factor (PF) showed 0.71±0.02 in the water extracts and 1.48±0.03 in the 60% ethanol extracts. The water extracts of Chamomaile leaves did not have antimicrobial activity against H. pylori, but the 60% ethanol extracts revealed the slight antimicrobial activity as 9.42 mm of clear zone. Angiotensin converting enzyme inhibition was 57.98% in water extracts and 91.36% in 60% ethanol extracts. Xanthine oxidase activity was 73.48% in water extracts and 81.96% in 60% ethanol extracts. The results suggest that Chamomailes extract may be useful as potential source as antioxidant, angiotensin converting enzyme and xanthine oxidase inhibitors.
천연물 / 유기화학 : 로즈마리(Rosmarinus officinalis L.) 추출물로부터 Helicobacter pylori에 대한 항균물질 분리 및 동정
윤소정 ( So Jung Yoon ),김진성 ( Jin Sung Kim ),조분성 ( Bun Sung Jo ),김정환 ( Jeung Hoan Kim ),이선호 ( Sun Ho Lee ),안봉전 ( Bong Jeun Ahn ),조영제 ( Young Je Cho ) 한국응용생명화학회(구 한국농화학회) 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.3
Rosmarinus officinalis extracts had a significant antimicrobial activity against Helicobacter pyori. Total phenolic contents and inhibition zone of rosemary extracts were estimated to be 25.7 mg/g and 14 mm at 200 μg/mL of phenolic contents, respectively. The Sephadex LH-20 and MCI-gel CHP-20 column chromatographic separations for the phenolic extracts from R. officinalis leaves led to isolation of five acids, whose structures were determined as protocatechuic acid (A), coumaric acid (B), caffeic acid (C), chlorogenic acid (D), and rosmarinic acid (E), from interpretation of spectroscopic data including nagative fast atom bombardment (FAB)-mass, 1H-NMR, 13C-NMR, and IR. All isolated compounds were tested for antimicrobial activity against H. pyori. The purified single compound showed less antimicrobial activity against H. pylori than the mixed purified compounds, which generate A+B, A+E, C+D, C+E (each 200 μg/disc) excellent as large clear zone by synergy effect. These results indicate rosemary extracts are preventive agents against H. pyori.