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        • KCI등재후보

          Beauveria bassiana 대량배양을 위한 탄소원, 질소원 및 고체 기질 선발

          김정준 ( Jeong Jun Kim ),한지희 ( Ji Hee Han ),이상엽 ( Sang Yeob Lee ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.4

          For mass production of entomopathogenic fungus Beauveria bassiana 149, isolated from moth larva, by two-phase fermentation, we performed selection of carbon and nitrogen sources for liquid culture and examined solid fermentation on carrier, ingredient, temperature, and water content. Spore production with rice powder, corn powder, and starch from sweet potato was higher than that of sucrose and dissolvable starch for liquid fermentation as first-phase fermentation. As a nitrogen source, addition of peptone and yeast powder showed higher spore production than NaNO3, fish powder, and soybean powder. The isolate produced more conidia in sawdust + wheat bran + corn powder, sawdust + wheat bran and rice shell + wheat bran as carrier and ingredient than vermiculite as carrier. Conidia production of B. bassiana 149 in solid-phase fermentation was twice higher at 30 than 20. Conidia yield was higher at 60% and 70% water content (26.9×108 and 38.6×108 conidia/g) than 40% and 50% (13.9×108 and 11.6×108 conidia/g), respectively.

        • KCI등재

          Bacillus thuringiensis 제품의 농도 및 혼합 조건에 따른 나방류 방제 효과

          김정준(Jeong Jun Kim),홍쭈(Hong Zhu),한지희(Ji Hee Han),이상엽(Sangyeob Lee),박홍현(Hong-Hyun Park),이상계(Sang Guei Lee) 한국농약과학회 2012 농약과학회지 Vol.16 No.4

          담배거세미나방, 파밤나방, 배추좀나방은 중요 경제 작물의 재배, 수확 시에 해를 입히는 해충들이다. 이들 해충의 방제를 위해 Bacillus thuringiensis를 이용한 Bt제는 여러 해 동안 화학 살충제의 대체 방제 수단으로 이용되고 있다. 국내에 시판 중인 미생물 살충제의 방제 효율 증진을 위해 Bt제의 추천 농도 이상과 제품 간 혼합에 따른 효과 평가를 실내 생물검정을 통해 수행하였다. 본 연구에 사용된 5 제품 모두 추천농도에서 배추좀나방 2령 유충에 높은 방제 효과를 보였으나, 파밤나방과 담배거세미나방에는 낮은 살충율을 보였다. 추천농도의 2배액과 4배액 처리 및 Bt 제품들의 혼합 처리에서도 파밤나방과 담배거세미나방에서는 방제 상승 효과는 없었다. Beet armyworm (Spodoptera exigua), diamondback moth (Plutella xylostella) and tobacco cutworm (S. litura) are insect pests causing damage in many economically important vegetables in Korea. Bacillus thuringiensis, commonly known as Bt, has been available as an alternative insecticide for many years. Five commercial Bt products and the mixtures evaluate the control efficacy against three kinds of moths. These commercial Bt products had high control efficacy against 2<sup>nd</sup> instar of diamondback moth, but didn"t show high mortality against beet armyworm and tobacco cutworm. Mixtures of Bt products didn"t have synergistic effects to the tested moths. Also application of twice and four times the recommended concentration didn"t improve the control effects against the tested three species of moths.

        • KCI등재후보

          단보 : 살균제가 곤충병원성곰팡이 Beauveria bassiana GHA 포 자발아 및 균사생장에 미치는 영향

          김정준 ( Jeong Jun Kim ),송유진 ( Yu Jin Song ),한지희 ( Ji Hee Han ),이상엽 ( Sang Yeob Lee ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.4

          This study was conducted to test effect of fungicides on the spore germination and mycelial growth of BotaniGard(R)(Beauveria bassiana GHA). Eight fungicides, which are using for plant disease control in cucumber cultivation, with different concentration were mixed with conidia of GHA isolate for 0.5, 2, and 4 hours. The fungicides trifluminazol, fenarimol, ethaboxam, copper hydroxide and tetraconazol did significantly not inhibit on spore germination and mycelial growth. The trifluminazol, propineb and tetraconazol on high concentrations inhibited the mycelial growth of GHA. However, mixing time of fungicide with conidia of GHA did not affect in spore germination and mycelial growth. These results suggest that fungicide effect needed be checked before mycopesticide is applied in the field.

        • KCI등재후보

          단보 : 곤충병원성 곰팡이 Beauveria bassiana 포자 발아촉진제가 복숭아혹진딧물 살충효과에 미치는 영향

          김정준 ( Jeong Jun Kim ),( Hong Zhu ),석순자 ( Soon Ja Seok ),이상엽 ( Sang Yeob Lee ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

          This study was conducted to investigate agents inducing conidial germination of an entomopathogenic fungus, Beauveria bassiana KK5. Different chemicals including carbohydrates were mixed with conidia of B. bassiana and incubated on water agar for 12 hours. Fructose, mannose and skim milk were useful for spore germination compared to other chemicals. Bioassays against green peach aphids were conducted with the fungal conidia suspended in 1% fructose, mannose and skim milk. Of them, a mixture of skim milk plus conidia of B. bassiana KK5 showed the highest mortalities against 3 rd instar of green peach aphid.

        • KCI등재후보

          관광동기와 관여도에 따른 관광목적지 매력성 연구

          김정준 ( Jeong Jun Kim ),류인평 ( In Pyoung Yoo ) 관광경영학회 2010 관광경영연구 Vol.44 No.-

          This study aimed at exploring the relationships of tourist`s motivation and involvement on a destination`s attractiveness by examining the five hypotheses derived from the related literature review. The results showed that tourist`s motivation had a positive influence on the level of involvement and the attractiveness of tourist destination, whereas physical motivation was found to have a negative influence on tour conditions. The level of involvement had a positive relationship with the attractiveness of tourism destination and tourist`s motivation, however, the level of importance was found to have a negative influence on tour conditions. The attractiveness of tourism destination was found to have a positive influence on satisfaction and intention to revisit while the satisfaction had a positive impact on intention to revisit. One limitation of this study to be addressed was that the study did not target the more specific and segmented markets. Second, this study sampled both domestic and overseas tourists for the purpose of convenience of sampling and market analysis. Therefore, in the future research further efforts should be made to develop more systematic framework by sampling specific targets at a specific destination for generalization of findings.

        • 原著(원저) : 수혈 대상 환자에서의 Gel card를 이용한 불규칙 항체 검출

          안효준 ( Hyo Jun Ahn(m. T) ),김정준 ( Jeong Jun Kim(m. T) ),보희 ( Bo Hee Kim(m. T) ),혜련 ( Hye Ryun Kim(m. T) ),영아 ( Young Ah Kim(m. T) ) 대한임상병리사협회 2001 임상수혈검사학회 발표자료집 Vol.6 No.1

          수혈 시 실시하는 교차시험 및 불규칙 항체선별 검사에서 환자가 불규칙 항체를 가지고 있는 경우 항체의 종류를 정확히 동정하여 환자에게 가장 적합한 혈액을 공급하는 것은 중요한 일이다. 이러한 목적으로 국민건강보험공단 일산병원 혈액은행에서 개원 후 지난 1년간 검출된 불규칙 항체에 대해 각 항체의 종류 및 빈도 등에 대해 분석하여 보았다. Gel card를 이용한 항체선별 검사에서 항-E 항체가 6건, 항- E 항체 및 항-c항체까지 검출된 경우가 4건, 항-Ce 항체가 2건, 항체선별 검사에서 양성이나 특이 항체로 검출이 되지 않은 복합 항체의 경우가 4건 등이었다. 불규칙 항체의 빈도(prevalence)는 0.64%이 었으며 대부분이 Rh-Hr 계열의 항체이며. 특히 Rh-Hr 표현형이 DCe인 경우의 환자에서 항-E항체 및 항-c 항체가 동시에 검출된 경우가 전체의 40%로써 국내에서 보고된 항체 검출률 보다 높은 수치였다. 본원에서 사용하는 불규칙 항체 검사법은 gel card를 이용 37도에서 시행하고 동정검사도 동일하여 이제까지 보고들과는 달리 Lewis 계열의 항체나 한냉항체는 드물었는데 이러한 항체는 그 임상적인 의미를 무시할 수 있는 항체이므로 본원과 같이 gel card를 사용 시 항체선별검사 및 동정검사에 효율적인 업무수행을 할 수 있으리라 생각된다. Irregular antibodies is all kind of antibodies except regular antibodies like anti-A or anti-B. The detection of irregular antibodies is very important to the patients with this antibodies for transfusion. Especially, Rh-Hr antibodies(anti-E, anti-c, anti-C, anti-e etc) have clinical significance in developing a delayed hemolytic transfusion reaction and hemolytic disease of the newborn especially. We reviewed identified irregular antibody in National Health Insurance Corporation Ilsan Hospital for one year(2000. 3-2001. 2). Antibody screening test using gel card identified irregular antibodies in 16 of 2482 cases. The frequency of irregular antibodies : anti-E, 0.24% anti-E+c, 0.16%, anti-Ce 2, 0.08%, specificity not determined, 0.16% in our study. Cold antibodies was not detected in gel card but identified in cross matching of saline phase. We use enzyme technique to detect some antibody like anti-c, anti-e, which could be missed in LISS Coombs phase. We detected 4 cases of anti-E+c and 6 cases of anti-E. The R1R1 patients(DCe) with anti-E were transfused by E+c antigen negative blood to prevent presentation of anti-c.

        • SCOPUSKCI등재
        • KCI등재

          추출용매에 따른 제주산 황칠나무의 항산화 활성과 지표성분 분석

          정준한(Jun-Han Jeong),진솔(Jin-Sol Kim),이현화(Hyun-Hwa Lee) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.12

          본 연구는 추출용매에 따른 황칠 잎과 줄기 추출물의 효능탐색을 위해 제주산 황칠 잎과 줄기를 이용하여 항산화 활성 및 지표성분 함량을 조사하였다. 제주산 황칠 에탄올추출물과 열수추출물의 총 폴리페놀함량, 플라보노이드 함량 및 DPPH 라디칼 소거능을 측정한 결과, 총 폴리페놀함량은 잎 100 ℃ 열수추출물에서 38.92 mg TAN/g, 줄기 60 ℃ 열수추출물은 39.97 mg TAN/g 로 열수추출물에서 유의적으로 높은 함량을 나타냈으며, 플라보노이드 함량은 잎 에탄올추출물에서 25.95 mg QUE/g과 줄기 60 ℃ 열수추출물에서 9.68 mg QUE/g으로 나타나 서로 다른 추출용매에서 유의적인 활성을 나타냈다. DPPH 라디칼 소거능 측정 결과, 잎과 줄기 모두 에탄올추출물에서 가장 우수한 DPPH 라디칼 소거 활성을 나타냈으며 또한 SOD, CAT, APX 항산화효소 활성에서도 에탄올추출물에서 유의적인 항산화 활성이 관찰되었다. 황칠의 폴리페놀 성분으로 알려진 hesperidin, chlorogenic acid와 항산화효능과 관련된 salicylic acid에 대한 지표성분 분석 결과, hesperidin과 chlorogenic acid의 함량은 잎, 줄기 모두 에탄올추출물에서 우수한 것으로 관찰되었고, salicylic acid의 함량은 121 ℃ 열수추출물에서 가장 우수한 것으로 나타났다. 이와 같이 제주산 황칠의 유의적인 항산화 효능과 지표성분 함량이 확인되어 향후 제주산 황칠 원료의 제품 표준화를 위한 기초자료로 활용될 수 있을 것으로 판단된다. In this study, we investigated the variance in the antioxidant activity and marker components of Dendropanax morbifera Leveille by extraction conditions and the consequent efficacy of its leaf and stem extracts. We assessed the total polyphenol and flavonoid content, DPPH radical scavenging activity, antioxidant enzyme activities [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) activity], and content of marker components (hesperidin, chlorogenic acid, salicylic acid) in the extracts of D. mobifera . Our results showed that the highest total phenolic content was found in the 100 ℃ leaf water extracts and 60 ℃ stem water extracts (38.92, 39.97 mg TAN/g, respectively). The highest flavonoid content was found in the leaf ethanol extracts and 60 ℃ stem water extracts (25.95, 9.68 mg QUE/g, respectively). The ethanol extracts showed the strongest antioxidant activity in the DPPH assay and antioxidant enzyme activities. The marker components determined by liquid chromatography-mass spectrometry (LC-MS/MS) analysis showed that the hesperidin and chlorogenic acid content of the ethanol leaf extracts and salicylic acid content of the 121 ℃ water stem extracts were the highest. These results indicate that the extracts of D. mobifera provide promising antioxidant effects and marker components. This provides basic data that can be used for the standardization of extracts in the future.

        • KCI등재

          고지방식이 마우스에서 콩잎추출물의 지방간 억제 효과

          소라(So Ra Kim),제지현(Ji hyun Je),정준연(Jun Yeon Jeong),현정(Hyun Jung Kim),송영훈(Yeong Hun Song),박기훈(Ki Hun Park),강상수(Sang Soo Kang),박상원(Sang Won Park) 대한약학회 2017 약학회지 Vol.61 No.3

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          This study was designed to investigate the effect of soybean leaf extract on the development of hepatic steatosis in high fat diet-fed mice. Soybean plants were treated with ethephon to enhance the production of isoflavones in the leaves. The mice were fed with normal chow diet(ND), high fat diet(HFD) or HFD+Soybean leaf extract for 9 weeks. HFD feeding significantly increased the body weight gain and visceral and subcutaneous adipose tissue weights compared to ND group in the mice, but these increases were attenuated by Soybean leaf treatment. Soybean leaf also attenuated the increases in hepatic lipid droplet and hepatocellular triglyceride level at 64 days of HFD feeding. Finally, soybean leaf markedly increased the phosphorylation of AMPK and ACC and their protein expression in the liver at 64 days of HFD feeding, while inhibited the increase in fatty acid synthase protein expression. Our results indicate that soybean leaf extract has beneficial effects against hepatic steatosis in HFD-fed mice.

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