들깨잎 폴리페놀 산화효소의 pH 및 온도에 의한 영향

김유경,김안근,Kim, Yoo-Kyung,Kim, An-Keun 대한약학회 2004 약학회지 Vol.48 No.6

Polyphenol oxidase-catalyzed oxidation of substrates (t-butylcatechol, 4-methylcatechol, chlorogenic acid, caffeic acid and pyrocatechol) were performed in the Ph range 4~8. Co ncentrations of substrate's major oxidation products were monitored by high performance liquid chromatograph. The nature and amounts of products formed were highly pH dependent. They also were ifluenced by kinds of substrates. Major oxidation product of 4-methylcatechol appeared the maxium value at pH 5, them of chlorogenic acid, caffeic acid and pyrocatechol at pH 6.0 and that of t-butylcatechol at pH 5~7. Time-dependent PPO activity was determined at $4^{\circ}C\;and\;30^{\circ}C$. PPO extracted by phosphate buffer containing triton X-114 (t-PPO) was more stable than PPO by phosphate buffer (b-PPO). The result of electrophoresis, at first PPO was showed only a band at 48 kd. After 1~3 days a partial degrade band was appeared in b-PPO and three partial degrade bands in t-PPO. No activity band was appeared in PPOs at $30^{\circ}C$ and b-PPO at $4^{\circ}C$ after 4 days. And a band (37 kDa) in t-PPO was remained finally and disappered. PPO from Perillae leaves has two activity bands at 48 and 37 kDa in previous paper. It was supposed that PPO in the leaves of Perilla frutescens was a protein having one molecular weight as 48 kDa. And 37 kDa protein, relatively proteolysis-resistant, was a proteolyzed form of a major form.

식품 섭취와 인지기능장애와의 관계: 체계적 문헌고찰 및 메타분석

김유경,김경희,Kim, Yoo-Gyeong,Kim, Kyung-Hee 한국식생활문화학회 2020 韓國食生活文化學會誌 Vol.35 No.6

This systematic review and meta-analysis aimed to determine whether food intake is effective in preventing diseases related to cognitive impairment. We searched English databases namely MEDLINE, PubMed and ScienceDirect from 2000 to May 2020, and Korean databases namely RISS, KISS, and DBPIA from 1990 to May 2020. We divided the data into 15 groups using the food group classification of the Korean Nutrition Society (KNS). The effect size (Cohen's d) was estimated using a random-effect model, and a 95% confidence interval was calculated for each study. We included 17 cross-sectional studies and 7 cohort studies which involved 45,115 participants. As a result of analyzing the subgroups in the Asian population of both sexes, it was observed that grain intake has a protective effect against cognitive impairment. For females, pulses and fish also have a protective role against cognitive impairment. In the case of seaweed, a negative relationship was found with a moderate protective effect against cognitive impairment (Cohen's d:-0.533, 95% CI: -0.939, -0.126; p=0.010) in Korean studies. Dairy products are associated with an increased risk of cognitive impairment in the American and European population but drinking alcohol is associated with a lower impairment risk. These results provide a basis for formulating the dietary guidelines for preventing dementia for each country.

신장 위구르무슬림 분리주의운동의 원인분석 연구

김유경 ( Yoo Kyung Kim ) 한국외국어대학교 중동연구소 2010 중동연구 Vol.29 No.2

Though the region of China known today as the Xinjiang Uyghur Autonomous Region has always been troubled, China has several reasons to retain the troublesome province. Xinjiang is important to China politically, economically, and strategically. China also wants to maintain hold of Xinjiang, fearing its loss would incite the secession of Taiwan and Tibet. For these reasons, Chinese government has set up a priority in developing economy in Xinjiang to rein in Muslim separatists. Chinese authorities often resort to heavy-handed suppression to the actions of nationalist separatism and religious extremism. The Xinjiang Uyghur Muslims, however, have mounted major riots and a series of bus bombings have taken in Xinjiang. The main driving force behind the riots is the East Turkistan independence movement. Historically the native population in Xinjiang have resisted Chinese dominance and inspired anti-Han riots from time to time. The conflict in this region has had several causes; the system of `regional autonomy` operating in Xinjiang must be seen as a principal source of the unrest. The pattern of economic development in Xinjiang has ensured the further stratification of the labor market, a stratification that is often blamed for aggravating inter-group friction and the gap between the rich and the poor. This paper analyzes the intrinsic sources of Uyghur discontent and ethno-national conflict in Xinjiang. To support this thesis, the paper presents a historical analysis of the dispute process in Xinjiang and examines the problem from various aspects-politics, economy, ethnicity and religion.

KUH 임무탑재시스템의 안전성설계 및 검증

김유경(Yoo-Kyung Kim),김명진(Myung-Chin Kim),김태현(Tae-Hyun Kim),임종봉(Jong-Bong Yim) 한국항공우주학회 2010 韓國航空宇宙學會誌 Vol.38 No.8

안전성 요구수준이 서로 다른 비행필수 데이터(Flight Critical Data)와 엄무필수 데이터 (Mission Critical Data)의 시현을 처리하기 위해 별도의 독립된 계기를 사용하지 않고 Glass Cockpit 설계를 적용하여 데이터를 통합처리하였다. 본 논문에서는 독립적으로 설계 진행되어온 비행조종계통과 임무탑재시스템의 통합설계를 위해 설계변경을 최소화하면서 비행조종계통에서 요구되는 비행필수 데이터처리의 안전성 요구수준을 만족시키는 최적화 설계를 제안하였다. 비행필수 데이터의 시현을 처리하기 위해 KUH 임무탑재시스템의 핵심구성품인 임무컴퓨터(Mission Computer)의 하드웨어 및 소프트웨어 설계변경을 최소화하였다. 임무탑재시스템의 안전성 요구도(Safety Requirement)를 검증하기 위한 시험절차를 개발하여 임무탑재시스템 통합시험장비(SIL)를 이용한 시험 수행 결과 안전성 요구도가 만족됨을 확인하였다. Integrated data processing for display of flight critical data and mission critical data was conducted without additional display instruments using glass cockpit design. Based on a pre-designed flight critical system and a mission critical system, this paper shows an optimal design of subsystem integration. The design satisfies safety requirements of flight control systems(FCS) and requires minimized modification of pre-designed systems. By conducting integration test using System Integration laboratory(SIL), it is confirmed that the introduced design approach meets the safety requirements of the MEP system.

제주도 한우 집단에서 Galactose mutarotase (GALM) 유전자의 삽입/결실 다형성과 도체형질의 연관성 분석

김유경(Yoo-Kyung Kim),이종안(Jongan Lee),신상민(Sang-Min Shin),고응규(Yeoung-Gyu Ko),유지현(Ji-Hyun Yoo),원미영(Miyoung Won) 한국생명과학회 2023 생명과학회지 Vol.33 No.12

이 연구는 제주도 한우집단에서 galactose mutarotase (GALM) 유전자형과 도체형질의 연관성을 시험하였다. GALM 유전자형은 3’-비해독부위(3’-UTR)의 14-bp (5’-GGTCTAATGACCAG-3’) 삽입/결실 다형성을 이용하였다. 한우 비육우 집단에서 GALM 유전자의 세 가지 유전자형(LL, LS, SS)이 모두 관찰되었다. 연관성 분석결과는 근내지방의 함량과 밀접한 상관을 보이는 육질등급과 근내지방도의 수준과, 등지방두께의 수준이 유전자형에 따른 유의적인 차이를 나타내었다(p<0.05). 동형접합인 SS 유전자형을 보유한 도체에서 LL 또는 LS 유전자형인 도체에 비해 근내지방 함량 수준은 더 높고, 등지방두께도 더 얇은 수준을 보였다. 반면, 도체중, 등심단면적, 육색, 지방색 등은 GALM 유전자형에 따른 유의적인 차이는 없었다(p>0.05). 3’-UTR에서 14-bp 절편의 결실은 RNA의 2차 구조의 변형과 RNA-결합 단백질, microRNA와의 결합능력에 대한 방해를 통해 유전자 발현에 영향을 줄 수 있는 것으로 예측되었다. GALM 유전자의 3’-UTR 영역에서 14-bp 삽입/결실 다형성에 대한 이번 연구결과는 소에서 근육과 등지방 조직에서 galactose 대사에 의한 지방 축적을 통해 성장형질, 도체형질에 영향을 주는 것으로 판단된다. This study examined the associations between the genotypes of the galactose mutarotase (GALM) gene and carcass traits in the Hanwoo population of Jeju Island, South Korea. The GALM genotypes were determined by the 14-bp (5’-GGTCTAATGACCAG-3’) insertion/deletion (InDel) polymorphisms of the 3′-untranslated region (UTR). All three genotypes (LL, LS, and SS) were found in the Hanwoo steer population. The association analysis showed significant associations between genotypes and several carcass traits, including traits related to intramuscular fat content, such as meat quality, marbling score, and backfat thickness (p<0.05). Animals harboring the SS genotype showed not only higher levels of intramuscular fat content but also lower levels of backfat thickness than animals harboring the LL and LS genotypes. On the other hand, no significant associations were found between the GALM genotypes and carcass weight, eye muscle area, meat color, or fat color (p>0.05). Deleting the 14-bp segment in the 3′-UTR resulted in the modification of the secondary structure of RNA and appeared to affect gene expression by interfering with the binding ability of GALM mRNA with RNA-binding proteins and microRNAs. These results suggest that the 14-bp InDel polymorphism in the 3′-UTR region of the GALM gene affects cattle growth traits and carcass quality through galactose metabolism–mediated fat accumulation in muscle and backfat tissues.

제주도에서 대륙쇠큰수염박쥐(Myotis aurascens)의 분포 현황 및 계통 유연관계

김유경(Yoo-Kyung Kim),박수곤(Su-Gon Park),김태욱(Tae-Wook Kim),박준호(Jun-Ho Park),프라딥 아디카리(Pradeep Adhikari),김가람(Ga-Ram Kim),박선미(Seon-Mi Park),이준원(Jun-Won Lee),한상현(Sang-Hyun Han),오홍식(Hong-Shik Oh) 한국생명과학회 2015 생명과학회지 Vol.25 No.12

본 연구는 제주도 서식이 명확히 확인되지 않았던 대륙쇠큰수염박쥐(M. aurascens)의 분포 현황과 계통 유연관계를 조사하기 위하여 수행되었다. 제주도 남서부의 진지동굴 3개 지역에서 대륙쇠큰수염박쥐 무리가 관찰되었다. 형태형질에 대한 비교결과에서 제주도 대륙쇠큰수염박쥐는 한반도 집단에 비해 HBL과 Hfu는 짧고, TL, EL, FAL, Tra는 더 길었다. 제주도 집단의 FAL, Hfcu는 유럽 집단보다 더 넓은 크기 변이를 나타내었다. 서식장소에 대한 주기적인 방문조사 결과, 같은 장소에서 대륙쇠큰수염박쥐들이 재확인이 되지 않았고, 이는 해안과 내륙의 진지동굴을 활동기 동안에 일간 휴식처로 이용하는 것으로 추정되었다. 본 연구에서 이용된 모든 제주도 박쥐들에서 두 가지 유전자(CYTB, COI)의 염기서열들이 모두 동일하게 나타나, 이들이 모두 동일한 모계의 후손들로 추정되었다. 분자 유전학적 연구결과는 제주도 대륙쇠큰수염박쥐의 서열들이 계통수 상에서 기존에 유럽과 아시아의 M. aurascens 서열들과 동일한 분지에 있었다. 뿐만 아니라, 제주도 집단에서 얻은 염기서열들은 한반도에서 보고된 대륙쇠큰수염박쥐의 서열과 가장 근연인 것으로 확인되었다. 결과적으로 본 연구를 통해 제주도에서 자연서식이 확인된 대륙쇠큰수염박쥐들은 모두 동일한 모계선조에서 기원하였고, 한반도나 다른 지역 집단들과는 형태적, 유전적으로 다른 배경을 보유하고 있어, 한반도를 경유하여 제주도로 이주해 온 집단으로 판단된다. 이상의 결과는 향후 제주도 포유동물의 유입경로와 생물지리학적 상관관계를 이해하는 데 있어 중요한 기초자료가 될 것이다. This study was aimed to investigate the distribution status and phylogenetic relationship of Myotis aurascens in Jeju Ialnd, which has not clearly confirmed until now. We found three groups of M. aurascens from three different cave enforcements (CEs). The bat population of Jeju Island had smaller levels of HBL and Hfcu, but greater levels of TL, EL, FAL, and Tra than those of the Korean Peninsula population. Jeju bats had wide range in the lengths of FAL and Hfcu comparing to those of European bats. From the bimonthly monitoring to each finding site, we have actually failed to observe those again, estimating that they use those CEs as the daily-roosting place in activating seasons. The sequences of CYTB and COI genes showed identical sequences among Jeju bats tested, indicating that they are maternally related. The results from molecular phylogeny showed that the sequences of these bats located on the same branch with those for M. aurascens in the phylogenetic trees. Besides, the nucleotide sequences of the Jeju bats showed the closest relation with that of Korean Peninsula. Consequently, these findings indicate that the bats of M. aurascens, verified the natural distribution in Jeju Island, have originated from a single maternal origin and differences in morphological and genetic backgrounds form those of Korean Peninsula and the other countries, and had probably immigrated via Korean Peninsula. These findings will contribute as basic information for understanding the migration history and biogeographic relationship of mammals on Jeju Island in East Asia.

인터넷 광고의 내용분석을 통한 국가간 비교 연구

김유경(Yoo-Kyung Kim),김은희(Eun-Hee Kim) 한국방송학회 2001 한국방송학보 Vol.15 No.3

제주도 큰발윗수염박쥐(Myotis macrodactylus)의 유전적 집단 구조와 계통 유연관계

김유경(Yoo-Kyung Kim),박수곤(Su-Gon Park),한상훈(Sang-Hoon Han),한상현(Sang-Hyun Han),오홍식(Hong-Shik Oh) 한국생명과학회 2016 생명과학회지 Vol.26 No.7

본 연구는 미토콘드리아 DNA (mtDNA) cytochrome B (CYTB)와 NADH dehydrogenase subunit 1 (ND1) 유전자 서열의 다형성을 근거로 제주도 큰발윗수염박쥐 집단의 유전적 집단 구조와 계통 유연관계를 조사하는 데 목적이 있다. 동아시아 박쥐에서 CYTB 유전자 haplotype은 14개의 haplotype들이 발견되었고, ND1은 9개의 haplotype들이 발견되었다. 집단별 haplotype의 분포는 지역-특이적인 양상을 보였다. ND1 haplotype 분석결과에서 제주도 집단은 4개의 haplotype을 나타내고, 한라산 소집단과 서부지역 소집단은 3개 haplotype을 나타내었으나, 동부지역 소집단에서는 제주도 전체에서 공통으로 발견되는 1개(Nd03)의 haplotype만 출현하였다. NJ tree에서 제주도 집단은 강원도 집단보다 일본 집단과 더 근연으로 확인되었다. 중국과 일본의 모계선조 계보 사이의 분화 시점은 0.789±0.063 MYBP으로 추정되었고, 제주도와 일본의 모계선조는 약 17만 년(0.168±0.013 MYBP) 전에 분리된 것으로 판단된다. 제주도 집단은 적어도 5만 년 이전에 이주한 것으로 보인다. 또한 ND1 haplotype 분석결과는 제주도 집단이 이주 후에도 지역 내에서의 적어도 2회 이상의 유전적 분화를 겪었다는 것을 보여주고 있다. 본 연구결과는 동아시아 큰발윗수염박쥐의 계통 유연관계를 이해하는 데 중요한 기초자료가 될 것이며, 향후 한반도의 남부와 중국, 러시아 등에서 시료 확보를 통해 집단 간 진화적 상관관계를 이해하는 데 필요한 설득력 있는 자료가 마련되어야 할 것이다. This study was carried out to reveal the genetic population structure of the Jeju Island population and the phylogenetic relationship of East Asian populations of the large-footed bat (Myotis macrodactylus) based on the genetic polymorphisms of mitochondrial cytochrome B (CYTB) and NADH dehydrogenase subunit 1 (ND1) gene sequences. A total of fourteen and nine haplotypes were found in the CYTB and ND1 sequences from East Asian bats, respectively. Haplotype distribution showed locality specific patterns. The results from ND1 haplotype analysis showed that the Jeju Island population has four haplotypes: the Mt. Halla and Western subpopulations have three ND1 haplotypes, but the Eastern subpopulation has just a single haplotype Nd03, which is commonly found on this island. The neighbor-joining (NJ) tree showed the closer relationship between Jeju Island and Japan rather than that between Jeju and Gangwon-do Province. The divergence time between the maternal ancestor lineages of Japanese and Chinese populations was estimated to be 0.789±0.063 MYBP. The secondary divergence between Jeju and Japanese bats was calculated about to be 0.168±0.013 MYBP. The Jeju population has immigrated to the island at least fifty thousand years ago. In addition, ND1 haplotype analysis suggested that the insular bats have experienced at least two further genetic differentiation events within this island. Consequently, these findings suggested that the results of this study may play a critical role in understanding the phylogenetic relationship among East Asian bat populations of M. macrodactylus. To prepare more explainable information on evolutionary correlation, analysis is still required to examine using expanded samples from China, Russia, and southern parts of the Korean Peninsula.

소아에서 발병한 human metapneumovirus와 respiratory syncytial virus에 의한 호흡기 감염의 임상 양상

김유경 ( Yu Kyung Kim ),김진우 ( Jin Woo Kim ),위영선 ( Young Sun Wee ),유은경 ( Eun Gyong Yoo ),한만용 ( Man Yong Han ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2009 소아알레르기 및 호흡기학회지 Vol.19 No.1

목적: 국내에서 호발하는 hMPV와 RSV 감염의 임상 양상의 차이가 아직 명확하지 않아 이를 확인하고자 한다. 방법: 2006년 8월부터 2007년 7월까지 분당차병원에 하부 호흡기 감염으로 입원한 환아 1,104명을 대상으로 비인강 흡입법과 RT-PCR을 이용하여 바이러스를 검출하였다. 이중 hMPV와 RSV 감염 양성인 환자 각각 51명과 138명을 대상으로 후향적인 의무기록분석을 통해 검출 시기, 연령, 성별, 임상 진단명, 임상 양상 및 검사 소견을 비교 분석하였다. 결과: hMPV감염은 4월에, RSV감염은 11월에 가장 많이 발생하였다. 두 군 모두 1세 미만의 영아가 가장 많았으나 6개월 이하의 군에서 hMPV는 25% RSV는 40%를 차지하였다. 2세 이상의 군에서는 hMPV가 33.4%, RSV는 19%였다. 진단명은 두 군 모두 폐렴, 급성 세기관지염, 급성 기관지염 순으로 차이를 보이지 않았다. 말초 혈액 백혈구수는 RSV 감염시 더 높았고, 입원 당시 열이 있는 환아의 비율은 hMPV군에서 더 높았다. 결론: 본 연구에서 hMPV는 영유아에서 바이러스성 하부 호흡기 감염의 네 번째로 흔한 원인으로, hMPV 감염의 임상양상은 RSV 감염과 유사하였다. 향후 이러한 연구가 호흡기 바이러스 감염의 효율적인 관리에 도움이 될 것이라 여겨진다. Purpose: To identify the clinical features of human metapneumovirus (hMPV) and the respiratory syncytial virus (RSV) infection in children. Methods: The participants of our study were 1,104 children who were admitted to Bung-dang CHA hospital for lower respiratory infection from August 2006 through July 2007. Nasopharyngeal swabs were taken from the patients, and viruses were identified by RT-PCR. The clinical features of 51 patients with hMPV infection and 138 patients with RSV infection were compared by retrospective review of their medical records. Results: The peak incidence of hMPV infection was noted in April, and that of RSV was noted in November. Both viruses had the highest incidence in patients age <1 year and hMPV infections occurred in 40% and 25% of patients age <6 months, respectively. In the hMPV group, 33.4% of the patients were age ≥2 years, while in the RSV group, 19% were age ≥2 years. In both groups, pneumonia was the most common clinical diagnosis, followed by acute bronchiolitis, acute bronchitis, and asthma. The white blood cell counts were higher in the RSV group, and fever was more frequent on admission in the hMPV group. Conclusion: hMPV was the fourth most common virus causing lower respiratory tract infections in children. The clinical features of hMPV infection were similar to those of RSV infection. This study may be helpful for the effective treatment of lower respiratory tract infection in children. [Pediatr Allergy Respir Dis(Korea) 2009;19:12-19]

은염과 디지털 도구에 따른 조명비 교육의 실제 적용에 관한 연구

김유경 ( Yoo Kyung Kim ) 한국사진학회 2010 AURA Vol.0 No.22

Photographers deal with light in the limited area of visible ray and the difference in techniques to treat the given light makes difference in works of them. They sometimes have to determine the directions, angles, colors, and quality of light. Also, they know there is lighting ratio in any light that reaches subjects in any situation. The most difficult part of taking pictures in digital environment is that the rule of lighting ratio appled when using silver halide(film) to create the desirable mood is not adequate. Thus, I reviewed a thesis on this part as a preliminary study and found out that when the ratio of highlight and shadow are 1:1, 1:2, 1:4 the results of silver halide were nearly similar with those of digital but the depth of shadow of digital turned out to be opposite in case of 1:8, 1:16, 1:32. In case of digital, I could get results of lighting ratio learned in silver halide only when l took pictures with sequentially reduced light intensity of fill light so that it might be less than the value of lighting ratio indicated on exposure meter, comparing to silver halide. Therefore, this study intends to review the phenomenon and value of education appeared when the results of preliminary study were applied to lighting education. The education was implemented, divided into two groups; one group that experienced the results from the process of comparison experiment of silver halide and digital in real education were different and the other group that didn`t. In addition, I intended to understand the difference in representation properties of silver halide and digital and present the necessity of differentiated and proper approach toward lighting ratio education by analyzing results through questionnaire survey.