테논낭 섬유아세포의 증식에 대한 NO의 역할

김근해 ( Keun Hae Kim ) , 김재우 ( Jae Woo Kim ) 대한안과학회 2003 대한안과학회지 Vol.44 No.7

Gelrite가 배양된 섬유아세포의 증식에 미치는 효과

김재우 ( Jae Woo Kim ) , 김근해 ( Keun Hae Kim ) , 진용헌 ( Yong Hun Jin ) 대한안과학회 2003 대한안과학회지 Vol.44 No.2

뇌조조영술 후 발생한 양측 활차신경마비 1예

김근해,윤숙현,김숙영,Keun Hae Kim,MD,PhD,Sook Hyun Yoon,MD,Sook Young Kim,MD,PhD 대한안과학회 2014 대한안과학회지 Vol.55 No.1

Purpose: To report a case of bilateral trochlear nerve palsy following cisternography. Case summary: A 43-year-old male with intermittent watery rhinorrhea persisting for 3 months visited the neurosurgery department of our institute. His past medical history included removal of a pituitary adenoma 22 years prior to presentation. Cerebrospinal fluid leakage was suspected and cisternography was performed. The patient was referred to our ophthalmology department for diplopia 3 days after the cisternography. An alternate prism cover test showed 5 prism diopter (PD) right hypertrophia in the primary position, and underaction of bilateral superior oblique muscles and overaction of the left inferior oblique muscle. A positive Bielschowsky test with the head tilted to either side was observed and excyclotorsion was 9° on the double Maddox rod test. The patient was diagnosed with bilateral trochlear nerve palsy. After 2 years of follow- up, diplopia persisted and recession of the bilateral inferior oblique muscles was performed. After the surgery, diplopia disappeared, the fundus photography showed no excyclotorsion, and the double Maddox rod test indicated 3° of excyclotorsion. Conclusions: Cisternography should be carefully performed due to the possibility of bilateral trochlear nerve palsy, an extremely rare but possible occurrence following the procedure. J Korean Ophthalmol Soc 2014;55(1):155-160

사시 수술 중 근부착부의 위치 변화

김근해,김숙영,Keun Hae Kim,Sook Young Kim 대한안과학회 2006 대한안과학회지 Vol.47 No.1

Purpose: To study how far the insertion site of the extraocular muscle is displaced during strabismus surgery. Methods: We studied 69 eyes of 36 patients for concomitant exodeviation. The distance between the center of the extraocular muscle insertion site and the nearest posterior limbus was measured before and after detaching the lateral rectus muscle. After fixating the insertion stump with forceps, the distance between the same points was measured. Results: The distance between the limbus and the insertion site was measured as 6.54±0.65 mm before detaching the lateral rectus, 6.39±0.65 mm after detaching the muscle from the insertion, and 6.30±0.64 mm fixation of the insertion stump with forceps. Therefore, the muscle insertion site became displaced by 0.15±0.18 mm anteriorly from its original site after disinserting the muscle, and displaced anteriorly by a total of 0.25±0.22 mm after fixating the insertion stump with forceps. Differences in the distance at each stage were statistically significant (p<0.01, paired t-test). Conclusions: The insertion site of the lateral rectus muscle may be displaced anteriorly after disinserting the muscle and fixating the insertion stump with forceps. Therefore, the probability of error in strabismus surgery should be considered.

배양된 사람 코점막 섬유아세포에 대한 마이토마이신 C와 5-플루오로우라실의 효과

김근해,김인택,Keun Hae Kim,In Taek Kim 대한안과학회 2011 대한안과학회지 Vol.52 No.2

Purpose: To investigate the effects of two antimetabolites, mitomycin C (MMC) and 5-fluorouracil (5-FU), on proliferation of cultured human nasal mucosa fibroblasts. Methods: Human nasal mucosa fibroblasts were primarily cultured, and exposed to various concentrations of MMC and 5-FU for 5 minutes. Control fibroblasts were exposed to only DMEM media without the drugs. Effect of drugs on cell morphology was observed by phase-contrast microscopy. Cell viability and apoptosis were measured using MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide] assay and Acridine orange/Hoechst (AO/HO) staining, respectively. Results: In both experimental groups exposed to MMC and 5-FU, fibroblasts maintained standard spindle shape. The MTT assay showed that both MMC and 5-FU inhibited fibroblast proliferation in a dose dependent manner. AO/HO staining showed apoptotic cells in both experimental groups. Conclusions: Both MMC and 5-FU have an antiproliferative effect on fibroblasts in vitro at least through induction of apoptosis. Therefore, adjuvant use of either MMC or 5-FU during endonasal dacryocystorhinostomy may improve the clinical outcome by inhibiting proliferation of the nasal mucosa. J Korean Ophthalmol Soc 2011;52(2):233-240

국소마취하 눈꺼풀처짐 교정수술 중 발생하는 안구심반사

강현구,김근해.Hyun-Gu Kang. M.D.. Keun-Hae Kim. M.D. 대한안과학회 2008 대한안과학회지 Vol.49 No.1

Purpose: To evaluate the incidence and examine the development of oculocardiac reflex during ptosis operation under local anesthesia. Methods: Twenty-eight patients (52 eyes) who underwent ptosis operation under local anesthesia participated in this prospective study. We examined the change of heart rate. We also investigated the incidence of oculocardiac reflex and analyzed factors associated with it. Results: The mean preoperative heart rate was 76.06±11.24 beats/min. During local anesthetic injection, skin incision, traction of the central fat pad, traction of the medial fat pad, traction of the levator palpebrae muscle, the mean heart rates were 74.81±12.64 beats/min, 74.73±12.20 beats/min, 73.63±11.73 beats/min, 73.35±12.24 beats/min, 71.44±11.93 beats/min respectively. Hence, the mean heart rate decreased during each successive part of the operation. The oculocardiac reflex was positive in 24 (46.2%) of 52 eyes. There was no significant difference between the incidence of oculocardiac reflex in males and females. The incidence of oculocardiac reflex was highest during traction of the levator palpebrae muscle. Conclusions: During ptosis operation under local anesthesia, oculocardiac reflex can occur. Gentle manipulation of surgical tissues and cardiac monitoring is needed to prevent side effects from oculocardiac reflex.

안와에 발생한 결막 유피낭종 1예

이승희,김근해,김숙영,Seung-Hee Lee,MD,Keun-Hae Kim,MD,Sook-Young Kim,MD 대한안과학회 2010 대한안과학회지 Vol.51 No.4

Purpose: To report a case of primary conjunctival dermoid of the superonasal orbit. Case summary: A 41-year-old man complained of swelling of the left lower eyelid and left periocular pain for a week. Examination revealed 3 mm of proptosis with superotemporal displacement of the left eye. Orbital CT revealed a 32×27×33-mm well-defined giant cyst with a fat-fluid level in the superonasal aspect of the left orbit. Orbital MRI showed bone remodeling around the cyst, consistent with a dermoid cyst. The cyst was approached via lateral orbitotomy and transcaruncular incision but was ruptured just prior to the end of the dissection and was totally excised using a cryoprobe to freeze the ruptured site. Upon histopathological examination, the cyst was misdiagnosed as a conjunctival cyst because there was no dermal appendage but was rediagnosed as a conjunctival dermoid cyst after the tissue sample was examined more thoroughly. After surgery, the patient presented with diplopia due to esodeviation and was prescribed prismatic lenses. J Korean Ophthalmol Soc 2010;51(4):601-605

복시를 주소로 내원한 에드하임-체스터병 1예

김세은,김숙영,김근해,Se Eun Kim,Sook Young Kim,Keun Hae Kim 대한안과학회 2014 대한안과학회지 Vol.55 No.2

Purpose: We present a case of Erdheim-Chester disease (ECD) with diplopia. Case summary: A 56-year-old woman came to the hospital with a 6-week history of diplopia on left lateral gaze. The right eye showed mildly limited adduction. Humphrey automated perimetry demonstrated inferior bitemporal quadrantanopia. Orbital and brain magnetic resonance imaging revealed well-defined orbital masses in both intraconal orbits with homogenous enhancement, as well as multiple masses of homogenous signal intensity in the brain. Systemic evaluation showed involvement of the long bones, and retroperitoneum, but no involvement of the heart, or lungs. Incisional biopsy of the right orbital mass was performed. Histopathological examination showed numerous lipid-laden histiocytes and few multinucleated Touton giant cells. Immunohistochemical staining showed positivity for CD68, but negativity for CD1a, and ECD was therefore diagnosed. The patient received treatment with radiation therapy and interferon-α, but died due to sepsis secondary to urinary tract infection after 2 months. Conclusions: Except exophthalmos, diplopia may be the only initial symptom of an orbital mass. Although rare, the possibility of ECD should be considered in the differential diagnosis of both retrobulbar and orbital masses with diplopia. J Korean Ophthalmol Soc 2014;55(2):283-288

프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향

김재우,김윤영,김근해,Jae Woo Kim,M,D,Yoon Young Kim,M,D,Keun Hae Kim,M,D 대한안과학회 2005 대한안과학회지 Vol.46 No.1

Purpose: To investigate whether prostaglandin F2α (PGF2α) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. Methods: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2α (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, Nω- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. Results: PGF2α enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2α was not cytoprotective in serum-deprived and hypoxic conditions. Conclusions: The current results suggest that PGF2α potentiates NO production but is not cytoprotective in CM cells. This PGF2α-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow.

섬유주세포에서 Rho Kinase 저해제가 일산화질소의 생성에 미치는 영향

김재우(Jae Woo Kim),김근해(Keun Hae Kim),황석진(Seok Jin Hwang) 대한안과학회 2016 대한안과학회지 Vol.57 No.4

목적: 섬유주세포에서 Rho kinase (ROCK) 저해제가 일산화질소의 생성과 일산화질소합성효소(endothelial nitric oxide synthase, eNOS)의 발현에 미치는 영향을 알아보고자 하였다. 대상과 방법: 사람의 섬유주세포를 일차배양한 후 ROCK 저해제인 Y-27632를 0 μM, 10 μM, 100 μM의 농도로 노출시킨 후 1일, 3일째에 Griess assay를 이용하여 일산화질소의 생성량을 측정하였다. 노출 1일째에 섬유주단층세포층의 투과도와 교원질 겔의 수축에 미치는 영향을 조사하였고, reverse transcription-polymerase chain reaction (RT-PCR)을 이용하여 eNOS mRNA의 발현 정도를 측정하였다. 결과: 10 μM, 100 μM 농도의 Y-27632는 24시간 노출 후 섬유주단층세포층의 투과도를 농도에 비례하여 유의하게 증가시켰으며(all p=0.001), 교원질 겔을 이완시켰다. 또한 Y-27632는 1일과 3일째 모두 일산화질소의 생성량을 증가시켰으며(각각 p=0.020, 0.001), eNOS mRNA의 발현도 함께 증가하였다(p=0.039). 결론: 섬유주세포에서 ROCK 저해제가 섬유주를 통한 방수유출을 증가시키는 기전에 있어서 일산화질소의 생성 증가가 관여할 것으로 생각된다. Purpose: To investigate the effects of Rho kinase (ROCK) inhibitor on the production of nitric oxide (NO) and expression of endothelialnitric oxide synthase (eNOS) in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0 μM, 10 μM or 100 μM Y-27632 for 3 days and NO production was assessed using Griess assay. After 24 hours, the effect of Y-27632 on the contraction of collagen matrix and the permeability of the HTMC monolayer was determined. The expression of eNOS mRNA was assessed using reverse transcription-polymerase chain reaction (RT-PCR) and cellular survival with the 3-(4, 5 -dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results: In HTMC, 10 μM and 100 μM Y-27632 significantly increased NO production after 1 day and 3 days (p = 0.020 and 0.001, respectively). At 1 day after exposure, Y-276320 significantly relaxed the collagen matrix and increased the permeability of the HTMC monolayer (all p = 0.001) and the eNOS mRNA expression (p = 0.039). Conclusions: Increased NO production may play a role in the mechanism of increased trabecular outflow associated with ROCK inhibitor.