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우경미,김현만,고지승 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.5
Pro-oxidant properties of ascorbate have been studied with uses of brain tissues and neuronal cels. Here we address potential mechanism of ascorbate coupling with glutamate to generate ox-idative stres, and the role which oxidized as-corbate (dehydroascorbate) transport plays in oxi-dative neuronal injury. Ascorbate in neurones can be depleted by adding glutamate in culture med-ium since endogenous ascorbate can be ex-bate/dehydroascorbate transport by depleting as-corbate in the neurons with the glutamate-heter-oexchange. However, ascorbate is known readily being oxidized to dehydroascorbate in the med-ium. Glutamate enhanced the dehydroascorbate uptake by cels via a glucose transporter (GLUT) from extracelular region, and cytosolic dehydro-ascorbate enhanced lipid peroxide production and reduced glutathione (GSH) concentrations. Iso-as-corbate, the epimer of ascorbate was ineffective in generating the oxidative stress. These obser-vations support the curent concept that the high rates of dehydroascorbate transport via a GLUT after the release of ascorbate by glutamate leads to peroxidation, the role of glutamate on ascor-bate/dehydroascorbate recycling being critical to induce neuronal death via an oxidative stress in the brain injury.
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홍성호,한지은,고지승,도선희,이응호,조명행 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3
Atopic dermatitis, one of the most important skin diseases, is characterized by both skin barrier impairment and immunological abnormalities. Although several studies have demonstrated the significant relationship between atopic dermatitis and immunological abnormalities, the roleof hydroxyeicosatetraenoic acids (HETE) in atopic dermatitis remains unknown. To develop chiral methods for characterization of 12-HETEenantiomers in a 1-chloro-2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis mouse model and evaluate the effects of 12-HETEon atopic dermatitis, BALB/c mice were treated with either DNCB or acetone/olive oil (AOO) to induce atopic dermatitis, after which 12(R)-and 12(S)-HETEs in the plasma, skin, spleen, and lymph nodes were quantified by chiral liquid chromatography-tandem mass spectrometry. 12(R)- and 12(S)-HETEs in biological samples of DNCB-induced atopic dermatitis mice increased significantly compared with the AOOgroup, reflecting the involvement of 12(R)- and 12(S)-HETEs in atopic dermatitis. These findings indicate that 12(R)- and 12(S)-HETEs couldbe a useful guide for understanding the pathogenesis of atopic dermatitis.
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국내 사육중인 쿠바홍학에서 Aspergillus fumigatus 감염증례
김보숙,정영목,김한준,고지승,도선희 한국임상수의학회 2013 한국임상수의학회지 Vol.30 No.1
Aspergillus fumigatus (A. fumigatus) is one of the most important mycotic infection in wild animals stressed by captivity and concurrent disease. A 14-year-old Carribean flamingo controlled bumble foot with antibiotics died after having dyspnea, depression, and anorexia. Necropsy findings revealed pyogranulomatous inflammations of lung and greenish gray-colored mold on air sacs. A carribean flamingo was diagnosed as pyogranulomatous pneumoniae due to A.fumigatus infection by results of lung tissue culture and microscopic examination.
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Osteoprotegerin is present on the membrane of osteoclasts isolated from mouse long bones
우경미,최영님,고승희,고지승,오귀옥,김각견 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.5
Skeletogenesis occurs through either intramem-branous or endochondral ossification. In adition, some parts of the skeletal components maintain their cartilaginous characteristics throughout life without mineralization. Runx2 is known to be a cesses. In this study, we examined the expression patterns of two major isoforms of Runx2 in early skeletogenesis. During intramembranous bone for-mation, Runx2-type I (Runx2-I) was widely expres-sed in osteoprogenitor cels and active osteo-blasts, while Runx2-type II (Runx2-I) expression was stringently restricted to cels lining mineral-ized bones. Cels in permanent cartilage expressed colagen type II (Col-I) but never expresed Runx2 or Col-X. These permanent cartilages were wel Runx2-I was negative. In endochondral bone for-mation, Runx2 expression temporarily disappeared in Col-II-positive proliferating chondrocytes, but a secondary surge of Runx2-I expression occurred in the prehypertrophic zone before the minerali-zation of cartilage. Collectively, both Runx2 iso-forms showed very similar expression patterns in active bone forming areas; however, Runx2-I has an exclusive role in the early comitment stage of intramembranous or endochondral bone form-ing processes or in cels surrounding permanent cartilage.
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가톨릭대학교 성의교정 동물실험윤리위원회의 동물보호·관리프로그램에 대한 고찰
주영신(Joo, Young Shin),한서연(Han, Seo Yeon),고지승(Ko, Ji Seung) 가톨릭생명윤리연구소 2020 인격주의 생명윤리 Vol.10 No.1
전통적으로 가톨릭 기관은 연구윤리와 생명윤리를 중요시하여, 임상연구 뿐만 아니라 동물실험분야에서도 이와 관련하여 많은 노력을 기울여왔다. 이 논문은 가톨릭대학교 성의교정 동물실험윤리위원회와 실험동물연구실의 역사와 기관 내에서의 역할과 기능, 동물실험계획서의 발전사 및 윤리적인 동물실험을 위하여 노력한 과정 등을 담고 있다. 1990년대 후반에 조직된 실험동물연구실과 2006년 구성된 IACUC 전신인 동물실험위원회에서 시작되어, 2005년과 2015년에 진행된 두 차례의 시설 리모델링, 2017년의 우수동물실험시설 인증과 2018년 AAALAC International의 완전인증까지 13년 간 진행된 유무형의 노력들로 현재의 IACUC와 실험동물연구실이 이루어진 것이다. 동물실험계획서 양식의 변천사를 통해 IACUC의 심의절차의 변화를 살펴볼 수 있고, 이용자, 출입자, 실기, 관리자 교육 등 교육프로그램의 구성으로 동물실험 연구자에 대한 교육의 발전과정을 확인할 수 있었다. 또한, A memorial day, PAM, Medical Record 등의 프로그램을 통해 윤리적인 동물실험을 위한 IACUC와 실험동물연구실의 노력을 엿볼 수 있었다. 이러한 우리 시설의 성장사가 동물실험을 시작하는 기관에게 적절한 길잡이가 되기를 바라며, 이를 바탕으로 우리 기관이 더욱 발전하여 가톨릭기관의 이념에 따라 윤리에 기초한 창의적인 연구를 이룰 수 있기를 기대한다. Traditionally, Catholic institutions have focused on research ethics and bioethics, and have made a great deal of effort in this area as well as in clinical research. This paper describes the role and function of the IACUC and Department of Laboratory Animal (DOLA) in Catholic University, their history and the evolution, the process of effort for the animal research. Starting with the DOLA organized in the late 1990s, and the IACUC predecessor, the Animal Experiment Committee was formed in 2006. After twice facilities remodeling in 2005 and 2015, IACUC and DOLA accredited the Korea Excellence Animal laboratory Facility from Korea Food and Drug Administration in 2017 and acquired AAALAC International full accreditation in 2018. The current IACUC and DOLA have been completed with the effort of almost 20 years until the final certification. The changes in IACUC s protocol review were affirmed through the changes of the protocol form. And the development process of education was confirmed by the composition of education program such as user, tour, practical skill, manager education. In addition, IACUC and DOLA for the ethical animal research were shown through programs such as A memorial day, PAM and Medical Record. Based on this foundation, I hope that the history of our facility will be an appropriate guide for organizations that start animal experiments and our institution will further develop and carry out creative research based on ethics and the ideology of Catholic institutions.
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