갈색날개매미충(Pochazia shantungensis) 월동알 부화와 약충 발육에 미치는 온도의 영향

최덕수,고숙주,마경철,김효정,이진희,김도익,Choi, Duck-Soo,Ko, Sug-Ju,Ma, Kyeong-Cheul,Kim, Hyo-Jeong,Lee, Jin-Hee,Kim, Do-Ik 한국응용곤충학회 2016 한국응용곤충학회지 Vol.55 No.4

본 실험은 갈색날개매미충의 난괴 채집시기별 부화율, 2011년부터 2014년까지 부화율과 부화시기, 그리고 발육에 미치는 온도의 영향을 조사하였다. 갈색날개매미충 알은 11월부터 익년 3월까지 채집시기가 늦어질수록 부화율이 높아지는 경향이며, 부화기간은 차이가 없었다. 온도별 부화 소요기간은 15, 18, 21, 24, 27, $30^{\circ}C$에서 각각 51.2, 31.3, 24.8, 19.4, 17.1, 19.4일이었으며 $18{\sim}27^{\circ}C$ 범위에서 70% 이상의 부화율을 보였다. 전남 구례지역에서 갈색날개매미충 월동 알의 부화시기는 2011년보다 2014년에 9일이 빨라졌으며, 겨울철 평균온도가 높았던 해에는 부화율이 높아지는 경향이었다. 부화약충부터 5령 약충까지 온도별 발육기간은 18, 21, 24, $27^{\circ}C$(습도 40~70%, 광조건 14L:10D)에서 각각 82.8, 58.0, 45.8, 39.6일로 온도가 높을수록 발육기간은 짧아졌는데, $30^{\circ}C$에서는 4령 이후 모두 사멸하여 시험온도중 약충의 발육최적온도는 $27^{\circ}C$이었다. 알에서부터 4령 약충까지의 온도와 발육속도와의 관계식은 Y = 0.0015 X - 0.014이며, 발육영점온도는 $9.3^{\circ}C$, 유효적산온도는 693.3일도였다. 발육영점온도는 4령 약충이 $3.8^{\circ}C$로 가장 낮았다. This study investigates the hatching periods and hatchability of the eggs of Pochazia shantungensis at different collection times from 2011 to 2014, and the effect of temperature on the growth of P. shantungensis nymphs in an area of its outbreak. The hatchability of P. shantungensis eggs varies with their collection time; their hatchability in late November was higher than that in March of the next year, but no difference was observed in their hatching periods. The hatching periods of the eggs were 51.2, 31.3, 24.8, 19.4, 17.1, and 19.4 days at 15, 18, 21, 24, 27, and $30^{\circ}C$, respectively. The hatchability was above 70% at temperatures ranging from 18 to $27^{\circ}C$. The hatching time of the overwintered eggs in the Gurye region in Korea was reduced by 9 days from 2011 to 2014. The hatching rate was relatively higher when the average temperature in the winter season was relatively warmer. The dvelopmental periods of the first to fifth nymphs were 82.8, 58.0, 45.8, and 39.6 days at 18, 21, 24, and $27^{\circ}C$, respectively, at the relative humidity of 40~70%, and a photoperiod off 14 h light:10 h dark. The higher the temperature, the shorter the developmental period. At $30^{\circ}C$, all life stages after the fourth nymph died. Thus, the optimum growth temperature was estimated to be $27^{\circ}C$. For all life stages from the egg to the fourth nymph, the relationship between the temperature and developmental rate was expressed by the linear equation Y = 0.0015 X - 0.014. The lower developmental threshold was $9.3^{\circ}C$ and the effective cumulative temperature was 693.3 degree-days. The lower developmental threshold of approximately $3.8^{\circ}C$ was the lowest at the fourth nymph stage.

복숭아씨살이좀벌(Eurytoma maslovskii )에 의한 전남지역 매실 피해현황, 발생생태 및 방제적기

최덕수,고숙주,마경철,김효정,김도익,김현우,Choi, Duck-Soo,Ko, Sug-Ju,Ma, Kyeong-Cheul,Kim, Hyo-Jeong,Kim, Do-Ik,Kim, Hyeun-Woo 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.3

복숭아씨살이좀벌(Eurytoma maslovskii)에 의한 전남지역 매실 낙과피해는 해안지역인 완도, 신안, 여수, 무안을 제외한 전 지역에 발생하였고 평균 피해과율은 2013년 67%, 2014년 33.3%였다. 이 씨살이좀벌은 매실 씨방에서 노숙유충으로 월동을 하는데 7, 8월의 장마철을 지나며 생존율이 30% 내외가 되었다. 알은 길이 0.68 mm, 폭 0.29 mm의 유백색이며 장타원형으로 양 끝부분에 실모양의 돌기를 붙이고 있다. 다 자란 노숙유충은 길이 6.56 mm, 폭 3.18 mm이다. 번데기는 검정색 나용이고, 성충은 암 6.97 mm, 수 4.90 mm이고 암컷의 산란관 길이는 0.64 mm 였다. 성충 우화시기는 4월 상순(매실 직경 4 mm)~5월 상순(17 mm)이고, 우화 최성기는 4월 중순이었다. 성충의 평균수명은 13.5일이고, 암수성비는 45.9 : 54.1이다. 과일당 평균 산란수는 1개 61.5%, 2개 30.8%이고 최대 4개까지 산란하였지만 동종포식에 의해 최종적으로 1마리의 유충만 서식한다. 성충의 매실에 산란시기는 4월 하순(직경 12~16 mm)이며 그 후에는 씨방벽이 딱딱하게 경화되어 유충이 씨방으로 침투가 어려워진다. 따라서 성충의 산란을 예방하기 위한 방제적기는 4월 중순부터 5일 간격으로 2~3회 적용약제를 살포하는 것이다. Fruit drop due to Eurytoma maslovskii infestations of Japanese apricot (Prunus mume) is a serious economic issue in most parts of Jeonnam Province, with the exception of the coastal areas such as Wando, Shinan, Yeosu, and Muan. The average incidence of fruit drop was 67% in 2013 and 33.3% in 2014. E. maslovskii larvae overwinter inside the pits of Japanese apricots. Larval survival rates decrease to less than 30% after a rainy summer season. The eggs are long oval of 0.68 mm, 0.29 mm and ivory white. Mature larva is of 6.56 mm, 3.18 mm. Free pupa are black, adult females and males of 6.97 mm, 4.90 mm lengths, respectively, while the ovipositor is 0.64 mm in length. Adult emergence occurs from early April to early May, when Japanese apricot fruits are from 4 mm to 17 mm in diameter. Adults persist for 13.5 d after emergence, and the sex ratio of females to males was found th be 45.9:54.1. Although up to four eggs may be oviposited per fruit, only one larva will ultimately survive, ans the larvae are cannibalistic. The period during which E. maslovskii is able to oviposit on Japanese apricots only lasts from mid- to late April (fruit diameter: 12~16 mm). When the diameter of the fruit is >16 mm, the pit of the Japanese apricot hardens and larvae have difficultly penetrating the stone. Therefore, the most effective method of controlling this pest is to spray orchards with a control agent 2~3 times, at 5 d intervals, beginning in mid- April.

무농약 토마토재배에서 친환경자재와 담배장님노린재를 이용한 온실가루이 방제

김도익,고숙주,최덕수,강범룡,김선곤,최경주,김상수,황인천,Kim, Do-Ik,Ko, Sug-Ju,Choi, Duck-Soo,Kang, Beom-Ryong,Kim, Seon-Gon,Choi, Kyeong-Ju,Kim, Sang-Soo,Hwang, In-Cheon 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.1

무농약 재배 토마토에서 발생이 많은 온실가루이를 방제하기 위해 유기농자재를 이용하여 방제한 결과와 천적 방사시기 및 천적과 유기농자재를 적절하게 이용하기 위해 유기농 자재가 담배장님노린재에 미치는 영향을 조사한 결과는 다음과 같다. 친환경농자재를 1주 간격 3회 연속 살포하면 $Beauveria$ $bassiana$, 고삼추출물, 제충국추출물, 멀구슬+고삼추출물, 고삼+계피추출물, 고삼추출물2가 70% 이상의 방제가를 보였다. 온실가루이 밀도가 높아진 이후 담배장님노린재를 방사하면 정착이 잘 되지 않아 방제가가 59.7%로 낮아 발생초기인 정식 1주일째부터 방사한 경우 5주째에 방제가가 90.8%로 높게 나타나 온실가루이를 방제할 수 있었다. 친환경 자재의 살포 방법별 담배장님노린재의 살충율은 살포 직후나 살포 후 약액이 다 마른 이후 천적을 방사하는 경우 살충율이 아주 낮았으나 방사이후 자재를 살포하면 67.7-70.5%의 살충율을 나타내므로 주의가 필요하였다. 그러나 해충인 온실가루이가 방제가 된 이후 담배장님노린재의 밀도가 급격히 증가하여 해충화 될 경우에는 멀구슬+고삼추출물과 제충국추출물, 고삼추출물을 살포할 필요가 있었다. Greenhouse whitefly, $Trialeurodes$ $Vaporariorum$, is the most important insect pest in environmental friendly tomato production. Natural enemies, $Nesidiocoris$ $tenuis$ and environmental friendly agricultural materials (EFAM) are major control agents in Korea. The aim of the present work is to evaluate release time of $N.$ $tenuis$ in controlling of $T.$ $vaporariorum$ and to select environmental friendly agricultural materials (EFAM) to suppress both $T.$ $vaporariorum$ and $N.$ $tenuis$ which if the density were high causing necrotic rings. Six EFAM selected to control of $Trialeurodes$ $Vaporariorum$ in three times spray with seven days intervals. Three EFAM showed high mortality 67.7-70.5% in spray after release plot but low in release after spray plot. It should be careful to spray with $N.$ $tenuis$. When $N.$ $tenuis$ were early release at 7 days after transplanting which before occurring and establishment of greenhouse whitefly, it could suppress the density. Extracts of bead tree + matrin, matrin 1, Chrysanthemum showed high mortality on $T.$ $vaporariorum$ adults and $N.$ $tenuis$. The results suggested that this three EFAM could be control agents of the damage of necrotic rings from $N.$ $tenuis$.

지표면 관리방법이 감 해충 발생과 생물다양성에 미치는 영향

최덕수,김도익,고숙주,강범용,박종대,최경주,김상수,Choi, Duck-Soo,Kim, Do-Ik,Ko, Sug-Ju,Kang, Beom-Ryong,Park, Jong-Dae,Choi, Kyeong-Ju,Kim, Sang-Soo 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.4

본 실험은 감과수원의 지표면 관리방법(녹비작물 재배, 초생재배, 청경재배)이 감 해충 발생량과 생물다양성에 미치는 영향을 조사하였다. 감 과수원의 녹비작물을 가해하는 해충은 알팔파바구미, 긴꼬리볼록진딧물, 아카시아진딧물 등 7종이며 가장 피해가 심한 해충은 알팔파바구미였으나 감나무에 피해를 주는 해충은 없었다. 과수원 지표면 피복식물에 서식하는 해충과 천적은 재배방법 즉 유기재배와 초생재배에 따라 발생량의 차이는 없었다. 차잎말이나방은 연 3회 발생최성기를 나타내는데 감나무에 피해는 경미하였으며 재배방법별 총 발생량은 유기재배 1,261마리, 관행초생재배 1,003마리, 청경재배 621마리로 청경재배 포장에 비해 지표면에 식물체가 존재하는 포장에서 발생량이 많았다. 복숭아순나방은 연 4회 발생하였는데 역시 감나무에 피해는 극히 적었다. 재배방법별 토양내 서식 미소동물 종과 개체수는 유기재배 22종 838개체, 초생재배 17종 421개체, 청경재배 15종 381개체였으며 종 풍부도는 유기재배 3.12, 초생재배 2.65, 청경재배 2.36으로 유기 > 초생 > 청경 순으로 개체수와 풍부도가 높았다. This study was investigated to evaluate the effects of biodiversity and pest insect occurrence by soil surface management methods such as green manure crop, grass planting and clean-tilled crops on persimmon orchards. Green manure crop pests was investigated 7 species including Hypera postica, Laodephax striatellus, Megoura crassicauda, Aphis craccivora, Nephotettix cincticeps, Liriomyza sp., black spot and the most dangerous pest was H. postica but it did not damaged persimmon tree. Amount of pests and natural enemies dwelling soil covering plant was not difference between organic farming (hairy vetch planting) and conventional farming (natural weed). Homona magnanima occurred three times a year but it rarely damaged leaf of persimmon. Total adult moth catched by pheromone trap was 1,261 organic farm, 1,003 conventional farm and 621 clean-tilled farm. Grapholita molesta occurred four times a year but it also rarely damaged twig of persimmon. Number of collected invertebrate species and individuals on soil sample was follow as : 838 individuals of 22 species at organic farm, 421 individuals of 17 species at conventional farm and 381 individuals of 15 species at clean-tilled farm. The richness index was lined up by organic farm 3.12> conventional farm 2.65> clean-tilled farm 2.36.

유기와 관행재배 배 과수원의 무척추동물의 종 다양성 연구

김도익,김선곤,고숙주,강범용,최덕수,임경호,김상수,Kim, Do-Ik,Kim, Seon-Gon,Ko, Sug-Ju,Kang, Beom-Ryong,Choi, Duck-Soo,Lim, Gyeong-Ho,Kim, Sang-Soo 한국유기농업학회 2011 韓國有機農業學會誌 Vol.19 No.1

유기 배 과수원의 생물종 다양성을 조사하기 위해 토양 미소동물은 토양채취를 하였으며 지상부의 생물 조사는 pitfall 트랩, malaise 트랩, 유아등을 사용하여 채집하였다. 토양채취에서는 유기재배에서 37종 1,184마리, 관행 재배 28종 501마리였으며 pitfall 트랩에서는 각각 38종 648마리, 29종 440마리였다. malaise 트랩과 유아등에서는 각각 55종 650마리, 47종 508마리와 23종 201마리, 9종 42마리였다. 토양채취에서 톡토기목이 389마리로 많이 채집되었는데 관행재배지 보다 5배 이상 많이 채집되었으며 pitfall 트랩에서도 183마리로 3배 이상 채집되어 지표생물로 선발되었다. 토양미소동물의 유기재배지의 종 다양도는 3월에 1.956, 8월에 2.638로 관행의 1.426, 2.011보다 더 높아 유기 재배지에서 생물 다양성이 더 높았다. Pitfall 트랩에서 주로 출현한 종들은 거미류, 톡토기류, 딱정벌레였다. 딱정벌레 중에 관행재배지에는 점박이먼지벌레, 폭탄먼지벌레가 유기재배지에서는 큰넓적송장벌레가 지표생물이 될 가능성이 높았다. malaise 트랩에 의한 곤충종은 벌목, 파리목이 많이 채집되었으며, 그중에서 벌류는 고치벌과 맵시벌이 주를 이루었다. 지상부 생물의 종 다양성 조사 결과, pitfall 트랩의 종다양도가 유기재배지에서 2.952, 관행 2.587이었으며, malaise 트랩은 유기 3.120, 관행 2.398, 유아등은 유기 2.010, 관행 1.507로 나타나 malaise 트랩에서의 종 다양도가 가장 높았다. This research was carried out to investigate invertebrate fauna with organic and conventional pear orchards, which used four collected methods; soil sampling for soil microorganism, pitfall, malaise, and black light trap for over ground species. Collected species were 37 species, 1,184 individuals in organic and 28 species, 501 individuals by soil sampling in conventional pear fields. Those were 38 species, 646 individuals and 29 species, 440 individuals by pitfall trap, 55 species 650 individuals and 47 species, 508 individuals by malaise trap, and 23 species, 201 individuals and 9 species, 42 individuals by black light trap. Collembola was collected 389 individuals in organic which was 5 times than in conventional in soil sampling. In pitfall trap, that was 183 individuals which was 3 times. The diversity indices of organic pear orchards were 1.956 in May, 2.638 in August and those of conventional was 1.426 in May, 2.011 in August in soil sampling. In pitfall trap, the dominant species were spiders, collembollan, and coleopteran. Among Coleoptera, indicator insects for the evaluation of agricultural environment suggested were Eusilpha jakowelewi as organic pear orchard and Anisodactykus punctatipennis and Pheropsophus jessoensis as conventional. Malaise trap was collected dominant species as Diptera and Hymenoptera of Braconidae and Ichneumonidae. The diversity indices of organic pear orchards were 2.952, 3.120, and 2.010 in pitfall, malaise and black light trap in over ground invertebrate sampling. The highest diversity was in malaise trap. The higher diversity indices, the lower dominance indices.

갈색날개매미충(신칭, Ricania sp.)의 발생생태와 친환경 방제자재 선발

최덕수,김도익,고숙주,강범용,이관석,박종대,최경주,Choi, Duck-Soo,Kim, Do-Ik,Ko, Sug-Ju,Kang, Beom-Ryong,Lee, Kwan-Seok,Park, Jong-Dae,Choi, Kyeong-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.2

본 실험은 2011년 전남 구례지역의 산수유, 감, 밤 등에 대발생하여 피해가 심한 갈색날개매미충의 기주식물, 산란특성, 형태적 특징, 생활환을 조사하고 친환경 방제자재 선발을 위하여 수행하였다. 갈색날개매미충의 기주식물은 목본류 32종, 초본류 19종을 포함한 51종이였으며, 산란 선호식물은 산수유, 감나무, 밤나무, 두충나무, 때죽나무였다. 알은 길이 1.24 mm, 폭 0.55 mm 의 장타원형으로 1년생 가지 속에 난괴 당 평균 28.8개의 알을 산란하였다. 약충은 4회 탈피하는데 항문 주변에 x자 모양의 흰색 또는 노랑색 밀납물질을 형성한다. 전남 구례지역에서 연 1세대 발생하며 알로 월동하여 부화시기는 5월 중순~6월 상순, 약충시기는 5월 중순~8월 중순, 성충은 7월 중순~11월 중순이며 알은 8월 중순부터 산란한다. 갈색날개매미충은 산란에 의한 1년생 가지 고사, 양분흡즙으로 인한 생육저해, 그을음병 유발에 의한 상품성을 하락시키는 피해를 준다. 갈색날개매미충 약충과 성충 방제에 효과적인 친환경자재는 고삼추출물제와 천연식물추출물제로 살충율이 80% 이상이었다. An outbreak of Ricania sp. occurred in the Kurye, Jeonnam area in 2011. This outbreak damaged many kinds of fruit trees such as Cornus, Persimmon and Chestnut. This experiment was conducted to survey the occurrence ecology of Ricania sp. such as host plants, oviposition characters, morphological characters and life cycle, as well as to select environmental friendly control agents. Ricaina sp. host plants included 51 species such as 32 xylophytes, and 19 herbaceous plants. Ricaina sp. preferred Cornus officinalis, Diospyros kaki, Castanea crenata, Eucommia ulmoides, Styrax japonicus for oviposition. Adults laid eggs on new inner twigs with 28.8 eggs per egg-mass. Egg size was 1.24 mm(length), 0.55 mm(width) in an oval shape. Nymphs molted four times. Every nymph stage had an x shape of yellow or white beeswax around the anus. Overwintered eggs of Ricania sp. hatched from the mid May to early June. Nymphal periods were from mid May to mid August and adults appeared from mid July but spawning began in mid August. Ricania sp. damaged new twigs by oviposition and retarded growth by sucking nutrients and producing a sooty mold. Sophora and natural plant extracts were effective environmentally friendly agricultural materials used to control the nymph and adult Ricania sp. Mortality was > 80%.

유기재배 차나무의 주요 해충 종류와 유기농업자재의 살충 효과

김효정(Hyo Jeong Kim),고숙주(Sug Ju Ko),최덕수(Duck Soo Choi),이진희(Jin Hee Lee),마경철(Kyung Cheol Ma),김도익(Do Ik Kim) 한국차학회 2016 한국차학회지 Vol.22 No.4

This study was conducted to monitor the major pests occurring in organic tea fields and evaluate eco-friendly insecticidal agricultural materials. Eleven species of insect pests were identified over 2 year study period (2015-2016) in Boseong. Tetranychus kanzawai, Eriophyes chibaensis, Calacarus carinatus and Polyphagotarsonemus latus showed the highest peaks from April to June and after late-August. Therefore, management tactics are required in early-April and late-August. Toxoptera aurantii showed the highest peak in early-June and early-September. The major pests among them were Apolygus spinolae (Meyer-Dur) and Caloptilia theivora. High densities of A. spinolae and C. theivora were observed in May and in October, respectively. Moreover, the rates of leaf damage were 28.4% and 10.4%, respectively. The seasonal occurrence of C. theivora was monitored by using a sex pheromone trap, which revealed that C. theivora occurred six times a year from mid-April to mid-December. The peak occurrence of C. theivora was recorded in late September. The controlling effects of OAM containing sophora, neem, a mixture of sophora and neem against four pests (Tetranychus kanzawai, Eriophyes chibaensis, Calacarus carinatus, and Toxoptera aurantii) were more than 94%.

좁은가슴잎벌레 (Phaedon brassicae)에 의한 배추피해 및 온도별 발육기간

최덕수 ( Duck Soo Choi ),고숙주 ( Sug Ju Ko ),마경철 ( Kyeong Cheul Ma ),김도익 ( Do Ik Kim ),김현우 ( Hyeun Woo Kim ) 한국토양동물학회 2015 한국토양동물학회지 Vol.19 No.1

This study was conducted to investigate the morphological characteristics, occurrence ecology and damage of chinese cabbage by Daikon leaf beetle (Phaedon brassicae) which is a serious pests of brassicaceous vegetables in Jeonnam province. Egg length is 0.97 mm, width 0.42 mm, yellowish long oval type. Larva looks like a larva of ladybug, has many black spots on body and molt 3 times. Pupal length is 3.49 mm, width 2.23 mm, light yellow. The adult body size of a female is 3.96 mm, male 3.64 mm, both are shiny dark blue. Developmental periods from egg to adult was 30.8, 23.5, 19.5, 17.4, 16.2 days at 18, 21, 24, 27, 30℃ of constant temperature condition (14L-10D), respectively. As the temperature was higher, developmental periods were shortened. From 18 to 30℃ adult alive above 90 days. But the larva can not live above 33℃. Developmental zero point of egg, 1st larva, 2nd larva, 3rd larva and pupa was 0.11, 14.08, 3.88, 0.02, 0.11℃ respectively. The relationship between temperature and development rate was expressed by linear equation (Y=0.0024X- 0.0097, R(2)=0.9739). From egg to eclosion the developmental zero point was 4.04℃ and effective accumulative temperature was 407.6 days-degree. Adult and larva of Phaedon brassicae make hole by nibble away the leaves of Chinese cabbage. When inoculated 5 adults of Phaedon brassicae per pot, the number of ankertrass was 19, 65, 113 on 1, 2, 3 days after, respectively. Average number of ankertrass by adult was 5.9 a day. When low density of Phaedon brassicae damaged more than high density.

오이 생육단계별 관비농도가 생육과 토양에 미치는 영향

이숙재(Sook Jae Lee),임태곤(Tai Gon Im),김희권(Hee Kwon Kim),고숙주(Sug Ju Ko),임근철(Geun Cheol Lim),양승렬 한국생물환경조절학회 2006 한국생물환경조절학회 학술발표논문집 Vol.15 No.2

수출용오이 착과수 조절이 측지발생에 미치는 효과

이숙재(Sook Jae Lee),임태곤(Tai Gon Im),고숙주(Sug Ju Ko),김명석(Myeong Seok Kim),임근철(Geun Cheol Lim) 한국생물환경조절학회 2008 한국생물환경조절학회 학술발표논문집 Vol.17 No.1