원저 : 생쥐에서의 골도분촌에 의한 상응혈위

고형균(Hyung Kyun Koh) 대한침구학회 2001 대한침구의학회지 Vol.18 No.6

Objective : Modern acupuncture research often utilizes the methodology of animal experiment where animal acupoints(AA) are stimulated and the results observed. There hasn`t been any consensus on the AA locations yet, but it is integral for the locations to be defined in detail for any animal experiment. Methods : The review of 24 original articles published in the Journal of Korean Acupuncture and Moxibustion Society for recent one year which utilized animal experiment methodology revealed that AA are located primarily by the method of transposition from human acupoints. Only a few of those AA were defined in detail. ST-36, CV-12, GB-34, BL-23 were among the most frequently used AA. Results & Conculsion : Transpositional Ⅲ acupoint system of mouse is proposed, which includes 3 points of LU meridian, 4 of LI, 10 of ST, 8 of SP, 3 of HT, 4 of SI, 26 of BL, 2 of KI, 4 of PC, 3 of TE, I1 of GB, 6 of LR, 8 of CV and 19 of GV.

蜂鍼毒療法이 抗炎, 鎭痛 및 解熱에 미치는 效能에 關한 實驗的 硏究

高炯均(Hyung Kyun, Koh) 대한한의학회 1992 대한한의학회지 Vol.13 No.1

생쥐에서의 골도분촌에 의한 상응혈위

고형균,Koh Hyung-Kyun 대한침구의학회 2001 대한침구의학회지 Vol.18 No.6

Objective : Modern acupuncture research often utilizes the methodology of animal experiment where animal acupoints(AA) are stimulated and the results observed. There hasn't been any consensus on the AA locations yet, but it is integral for the locations to be defined in detail for any animal experiment. Methods : The review of 24 original articles published in the Journal of Korean Acupuncture and Moxibustion Society for recent one year which utilized animal experiment methodology revealed that AA are located primarily by the method of transposition from human acupoints. Only a few of those AA were defined in detail. ST-36, CV-12, GB-34, BL-23 were among the most frequently used AA. Results & Conculsion : Transpositional Ⅲ acupoint system of mouse is proposed, which includes 3 points of LU meridian, 4 of LI, 10 of ST, 8 of SP, 3 of HT, 4 of SI, 26 of BL, 2 of KI, 4 of PC, 3 of TE, I1 of GB, 6 of LR, 8 of CV and 19 of GV.

녹용사근환약침(鹿茸四斤丸藥鍼) 및 경구투여(經口投與)가 흰쥐의 성장(成長)과 지능발달(知能發達)에 미치는 영향(影響)

강기원,고형균,이윤호,Kang, Ki-weon,Koh, Hyung-kyun,Lee, Yun-ho 대한침구의학회 2003 대한침구의학회지 Vol.20 No.6

Objective: This study was designed to investigate effects of Nokyongsageunhwan and Nokyongsageunhwan aquapuncuture on body weight, body length, intellectual development Methods: We observed body weight, body length, intellectual development after feeding Nokyongsageunhwan and Nokyongsageunhwan aquapuncuture for thirty three days, and mRNA expressions of GHRF, Somatostatin, IGF-1, NPY were analyzed by RT-PCR method. Results: The results were summarized as follows. 1. Body length was significantly increased in Nokyongsageunhwan herbal acupuncture group compared to normal group and normal saline injection group on 4th week, 2. Lower part of hind leg length was significantly increased in Nokyongsageunhwan oral administration group compared to normal group on 3rd week, and it was significantly increased in Nokyongsageunhwan herbal acupuncture group and Nokyongsageunhwan oral administration group compared to that of normal group on 4th week. 3. As results of observing memory aquisition using Morris water maze system, there was significantly difference in Nokyongsageunhwan oral administration group compared to that of normal group on 4th week. 4. As results of observing retention using Morris water maze system, staying time was significantly increased in Nokyongsageunhwan herbal acupuncture group compared to that of normal group. 5. The somatostatin mRNA expression in the hypothalamus was significantly decreased in Nokyongsageunhwan oral administration group compared to that of normal group. 6. The IGF-1 mRNA expression in the hypothalamus is significantly increased in Nokyongsageunhwan herbal acupuncture group and Nokyongsageunhwan oral administration group compared to that of normal group. 7. The NPY mRNA expression in the hypothalamus was significantly increased in Nokyongsageunhwan oral administration group compared to that of normal group, and it was increased in Nokyongsageunhwan herbal acupuncture group compared to that of normal group, but there was no significant difference. Conclusions: According to the above results, it appears that Nokyongsageunhwan oral administration and Nokyongsageunhwan herbal acupuncture on acupoint GB34 and GB39 showed effects on the growth and the intellectual development of rats.

염좌(捻挫) 후유증(後遺症)에 대(對)한 봉약침(蜂藥鍼) 요법(療法)의 임상(臨床) 응용(應用)

권기록,고형균,Kwon, Gi-Rok,Koh, Hyung-Kyun 대한약침학회 1999 Journal of pharmacopuncture Vol.2 No.1

We have studied Bee Venom Therapy to treat Sequalae of Sprain, and obtained following results. 1. The treatment of sprain is using the effect of the affected part on Bee Venom Therapy. 2. For the treatment of sprain, it is often used the near acupuncture point of injury(近位取穴) or painful point to press(阿是穴). 3. Bee Venom Therapy is one of the powerful treatment, so we are using the sequalae of sprain in the case of not cured by Acupuncture, Cupping or another therapy. 4. Bee Venom injection is started a small quantity, and is increased slowly by degrees. 5. The immune response by Bee Venom Therapy is a sort of Allergy, and it is classified large delayed reaction. It is considered positive reaction. 6. We have selected Acupuncture Points from one to four by the degree of injury. 7. The period of treatment is some different from the degree of injury, but generally we have obtained good results to treat from 5 to 20 by Bee Venom Therapy.

약침용(藥鍼用) 봉독액(蜂毒液)의 국소독성시험(局所毒性試驗)에 관(關)한 연구(硏究)

이종석,고형균,김창환,Lee, Jong-Seok,Koh, Hyung-Kyun,Kim, Chang-Hwan 대한한의학회 1995 대한한의학회지 Vol.16 No.1

Pursuant to the Medical Product Safety Administration Guidelines for safety assessment of Korean bee vonom for herb-acupuncture, rabbits were used for skin, eye balls, subcutaneous and muscle irritation test. The results were as follows; 1. The skin irritation test of bee venom for herb-acupuncture did not produce any irritation reactions, when the skin was covered with bee venom. 2. The eye irritation test produced moderate to severe stimulating reactions, once the eye mucous membrane was contacted with bee venom. 3. The subcutaneous and muscle irritation test of bee venom showed such local inflammatory reactions as death of cells, infiltration of inflamed cells, dropsical swelling, and congestion, once injected under the skin and in the muscle. As the results of the study on bee venom for herb-acupuncture indicate that severe reactions in such tests as eye balls, subcutancous and muscle irritation exist in the laboratory, more professional caution should be taken in clinical application of the therapy. In addition, further study on subacute, chronic toxicity and allergy reactions should be pursued.

봉독약침자극(蜂毒藥鍼刺戟)이 뇌간(腦幹) 신경세포(神經細胞)와 Serotonin성(性) 신경세포(神經細胞)의 활성변화(活性變化)에 미치는 영향

김혜남,고형균,박동석,강성길,김용석,최용태,Kim, Hye-Nam,Koh, Hyung-Kyun,Park, Dong-Suk,Kang, Sung-Keel,Kim, Yong-Suk,Choi, Yong-Tae 대한침구의학회 2000 대한침구의학회지 Vol.17 No.2

This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of serotonergic(5-HT) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats, the number of Fos immunoreactive neurons was counted by using computerized image analyzing system. Also, the number of colocalization between 5-HT containing neurons Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1. In almost every neucli, the Chok-Samni group and Blank locus group showed more increase in the number of Fos immunoreactive neurons than the control group. Especially, in Arc, DR, LC, RMg, Gi, PAG Rost and PAG LV, the Chok-Samni group showed more significant increase than the control group. Also, in PAG LV Mid and Arc, Chok-Samni group showed more significant increase than the Blank locus group. 2. In DR and PAG LV Mid, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between 5-HT containing neurons and Fos immunoreactive neurons than the control group after the BV aqua- acupuncture. Also, the Chok-Samni group showed more significant increase than the Blank locus group. Consequently, the BV aqua-acupuncture increased more potent the number of Fos immunoreactive neurons and the activity of serotonergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as serotonin Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.

봉약침요법의 면역반응에 관한 임상적 연구

권기록,고형균,Kwon, Gi-Rok,Koh, Hyung-Kyun 대한침구의학회 2000 대한침구의학회지 Vol.17 No.1

The immune response of Bee Venom Therapy is commonly appear in clinics. It is whole body delayed allergy type and generally like fatigue. Therefore, in order to analysis the clinical form, we have observed immune response of 100 patients who visited Sangji University Oriental Medical Hospital and treated Bee Venom Therapy over 10 times from November 1998 to October 1999. The results were summarized as follows. 1. The distribution of Sex was 60 females, 40 males, and the average of patients age was $50.6{\pm}1.5years$. 2. The distribution of disease was degenerative arthritis, HIVD of L-spine, RA, etc. 3. The total treated time is 2765 and is observed 361 immume responses. 4. The average of keeping time in immume response is $11.8{\pm}0.6(hr)$, and the cases of over 24hrs is occupied 24.0%. 5. In the correlation between treated times and immume response is generally in inverse proportion. 6. The general aspect of immume response is chilling, heating, powerless, headache, dizziness, etc. 7. $M{\ddot{u}}ller$ Grade II-III was observed only 1%.

봉독약침요법(蜂毒藥鍼療法)이 항염(抗炎), 진통작용(鎭痛作用)에 미치는효능(效能)에 관(關)한 실험적연구(實驗的硏究)

권기록,고형균,Kwon Gi-Lok,Koh Hyung-Kyun 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

Purpose: In order to study the anti-inflammatory and analgesic effects of Bee Venom Therapy, writhing syndrome, carrageenin induced edema, and complete adjuvant induced arthritis were experimented. Methods: 1. We used rats of writhing syndrom induced by acetic acid injection. Group I was treated with Acupuncture therapy. Group II was treated with Bee Venom thearpy. we compared with Group I, II and the control group. 2. We used rats of paw edema induced by carrageenin injection. Group I Was treated with Acupuncture therapy, Group II was treated with Bee Venom thearpy we compared with Group I, II and the control group. 3. We used rats of arthritis induced by Freud's complete adjuvant injection. Group I was treated with Acupuncture therapy, Group II was treated with Bee Venom thearpy we compared with GroupI,II and the control group. Results: 1. Analgesic effects on writhing syndrome induced by acetic acid injection showed statistical significance in Bee Venom treated group as compared with the control group. 2. The paw edema induced by carrageenin injection was decreased with statistical significance in Bee Venom treated group as compared with the control group. 3. The arthritis induced by Freud's complete adjuvant injection wasn't significant. Conclusion: Bee Venom Therapy was effective on inflammatory disease.

PubMed 검색을 이용한 관절염 관련 봉독 연구에 대한 고찰

김태우,고형균,박동석,이재동,Kim, Tae-Woo,Koh, Hyung-Kyun,Park, Dong-Seok,Lee, Jae-Dong 대한침구의학회 2001 대한침구의학회지 Vol.18 No.5

Objective : To research the trends of the study related to bee venom and arthritis, and to establish the hereafter direction of the study on bee venom herval acupuncture. Method : We searched in PubMed, with bee venom and arthritis (with abstract). Results : 1. We searched15 Papers, and the pattern of the was as fallows: Experimental study(13 Papers), Epidemiologic study(2), Review study(1). 2. The type of the experimental studies(12 Papers) was as follows: In vivo(6 Paper), In vitro(6). And the involved components of bee venom were as follows: Bee venom itself(6 Papers), Melittin(3), PLAP(1), PLAP & Melittin(1), Adolapin(1). 3. Among the experimental studies(12 Papers), the mechanism of inflammation was 10 papers (Inhibition 7, Inducer 3), and the effect of bee venom herbal acupuncture therapy were 2 papers.