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Identification and Characterization of Nitric Oxide Synthase in Salmonella typhimurium
Choi, Don-Woong,Oh, Hye-Young,Hong, Sung-Youl,Han, Jeung-Whan,Lee, Hyang-Woo The Pharmaceutical Society of Korea 2000 Archives of Pharmacal Research Vol.23 No.4
The presence of the nitric oxide synthase (NOS) enzyme from Salmonella typhimurium (S. typhimurium) was identified by measuring radiolabeled L-$[^3H]$citrulline and NO, and Western blot analysis. NOS was partially purified by both Mono Q ion exchange and Superose 12HR size exclusion column chromatography, sequentially. The molecular weight of NOS was estimated to be 93.3 kDa by Western blot analysis. The enzyme showed a significant dependency on the typical NOS cofactors; an apparent Km for L-arginine of 34.7 mM and maximum activity between $37^{\circ}C$ and $43^{\circ}C$. The activity was inhibited by NOS inhibitors such as aminoguanidine and $N^{G}$ $N^{G}$-dimethyl-L-arginine. taken together, partially purified NOS in S. typhimurium is assumed to be a different isoform of mammalian NOSs.OSs.
여대생의 라이프 스타일과 스페셜티 스토어 이용실태에 관한 연구
권미린,박정화,백진이,안계환,안자영,오해정,현혜선 연세대학교 생활과학대학 1988 婦學 Vol.21 No.-
The purpose of this study was to find life styles and shopping trends at specialty store of college women students. Findings indicated that demographic variables such as economic and grade level were significantly related to shipping trends at specialty store.
Epiluminescence microscopy 를 보조적으로 이용하여 진단한 색소성 기저세포암 2 예
박재홍(Jae Hong Park),정증태(Jeung Tae Jeong),송해준(Hae Jun Song),오칠환(Chil Whan Oh),김일환(Il Hwan Kim) 대한피부과학회 2001 대한피부과학회지 Vol.39 No.10
Basal cell carcinomas(BCCs) are the most common skin cancers in Korea and a proportion of BCCs contain pigment. Pigmented basal cell carcinomas(PBCCs) are included in the differential diagnosis of invasive melanoma and other benign pigmented skin lesions(PSLs) because of their growth patterns and asymmetry of pigmentation. Epiluminescence microscopy(ELM) describes the non-invasive in vivo examination of skin lesions with a microscope using incident light delivered from an acute angle and oil immersion. Many studies have shown that epiluminescence microscopy can improve the diagnostic accuracy of PSLs. Menzies et al analyzed the morphologic features of a large set of 142 pigmented BCCs and produced a simple ELM method for diagnosis that would allow differentiation from melanomas and benign pigmented lesions. We observed morphologic features of two PSLs with a handheld 10 epiluminescence microscopy (Episcope, Welch Allyn Inc, Skaneateles Falls, NY). After the ELM examination, two PSLs were excised and processed for histopathology. The ELM findings and histopathologic diagnosis were compared for each lesion. ELM permits the recognition of two PBCCs and the fact that it is a non-invasive in vivo method makes it even more attractive as a diagnostic tool in clinical practice. (Korean J Dermatol 2001;39(10): 1127-1132)
Bo Young Park,Han A Cho,So Yeong Bang,Min Jeung Oh,Eun Ji Lee,Whan Hui Lee,Jae Min Joung,Mi Sook Yoon 대한치과의료관리학회 2023 대한치과의료관리학회지 Vol.11 No.1
Social phobia is a mental disorder that triggers physical reactions, such as cold sweats, headaches, and panic attacks, as a result of anxiety about a particular situation. Individuals with social phobia are thought to be more sensitive to dental anxiety and may have difficulty visiting the dentist as a result. This study aimed to determine the relationship between social phobia and dental fear among college students, who are reported to have a high prevalence of social phobia. A total of 120 survey responses were analyzed. To investigate the variation in dental fear levels based on the level of social phobia, the total social phobia score was divided into two groups: 41 points or more, and 40 points or less. The disparity in dental fear scores was then analyzed using a t-test. The study found that the average score for dental fear was statistically significantly higher in the group with a total social phobia score of 41 points or more compared to the group with a total score of 40 points or less (p<0.05). Furthermore, the group that scored 41 points or higher on the social phobia scale reported experiencing symptoms such as feeling nauseous at the dentist (3.29 points), sweating upon entering the dentist (3.13 points), and feeling afraid when looking at the dentist (3.13 points). The score was high (p<0.05). Therefore, dental patients exhibiting anxiety symptoms require a comfortable treatment environment to alleviate dental fear, and safe, pain-free dental treatment techniques must be employed.