피부 백혈병의 임상적 고찰

장인강 ( In Gang Jang ),이동원 ( Dong Won Lee ),한치화 ( Chi Wha Han ),김춘추 ( Chun Chu Kim ),조백기 ( Baik Kee Cho ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.4

Background: Leukemia cutis is readily recognized and documented by biopsy, in contrast. to leukemic involvement in more occult sites. Nine cases of leukemia cutis have been reported in the Korean literatures. However no collective clinical studies have been reported in Korea. Objective : We evaluated the differences in patient age and sex, the clinical appearences and distributions of the skin lesions, interval between diagnosis of systemic leukemia and skin involvement, clinical course, and prognosis according to the type of leukemias. Methods : We carried out a retvospective study of 22 cases of leukemia cutis. Clinical information was obtained from the records of of 22 patients diagnosed at St. Mary's Hospital from 1988 to 1995. All the included cases were well evaluated for their clinical and histopathologic findings. Results : 1. Among 22 patients with leukemia cutis, male patients outnumbered female by 2 to 1 and the mean age was 25.8 years. 2. The clinical appearance of leukemia cutis includes papules, macules, nodules, plaques in all types of leukemia. Ulcerative lesions and vesicles were seen infrequently in leukemia cutis. Leukemia cutis often involved saultiple location of the skin, with no specific predilection of the site. There were no differences in distribution of lesions depending on the types of systemic leukemia. 3. In 68% of the patients with leukemia cutis, the skin lesions developed after the systemic leukemia was diagnosed, and 14% of patients had concomitant, involvement. 18% of patients had skin lesions preceding the diagnosis of systemic leukemia, howevere cytochemical and cytomorphologic studies of bone marrow and peripheral blood smear were not employed at the time of the skin biopsy. 4. Fourteen of 22 patients(64%) did not achieve a complete remission following the diagnosis of leukemia cutis and two of 14 patients without having complete remission could achieve complete remissions with proper anticancer therapy after the diagnosis of leukemia cutis. Total eight patients(36%) achieved a complete remission, then they had a relapse of leukemia in the skin, without having had any skin involvement at the time of the diagnosis of leukemia. 5. Seventeen of 22 patients(77% ) who were being followed up in our series died after leukemia cutis was diagnosed. The mean intervals between diagnosis of leukemia cutis and death was 3.8 months and they died mostly within 1 year. Conclusion : The presence of leukemic infiltration in the skin may help the clinician suspect the early diagnosis and relapse of systemic leukemia. It appears that leukemia cutis is associated with a grave prognosis. (Kor J Dermatol 1996;34(4): 507-514)

혈액투석 환자에서 발생한 중심정맥 협착의 경피적 혈관성형술 및 스텐트 삽입술

양종오 ( Yang Jong O ),이상주 ( Lee Sang Ju ),박기현 ( Park Gi Hyeon ),장윤경 ( Jang Yun Gyeong ),강민규 ( Kang Min Gyu ),성인환 ( Seong In Hwan ),이강욱 ( Lee Gang Ug ),신영태 ( Sin Yeong Tae ) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.6

중심정맥 삽관의 부작용 중 중심정맥의 협착은 비교적 흔하여 쇄골하정맥 삽관을 시술 받은만성 신부전증 환자의 15-50%에서 발생한다. 중심정맥 협착 발생시 정맥압의 증가로 부종 등의 증상으로 같은 쪽 팔의 동정맥루를 사용할 수 없게 된다. 중심정맥 협착의 치료방법으로 경피적혈관성형술 및 스텐트 삽입의 임상적 의의를 알아보고자 저자들은 충남대학교병원에서 1999년 3월부터 2001년 2월까지 혈관조영술상 중심정맥 협착으로 진단 받고 경피적 혈관성형술 및 스텐트 삽입술을 시행 받은 10명을 대상으로 분석하였다. 대상환자 중 남자가 3명, 여자가 7명이었고, 평균 나이는 55(39-74)세이었다. 스텐트 삽입 후 추적기간은 중앙값 15.8(9.3-23.7)개월이었다. 만성 신부전증의 원인은 고혈압 4명, 당뇨병 4명, 원인 불명 2명이었다. 대상자 중 8명은 동정맥루이고 2명은 인조혈관을 이용한 동정맥 이식편으로 혈액투석 치료를 받고 있던 환자였다. 부종이 발생한 상완은 좌측이 9명, 우측이 1명이었고 모두 환자의 동정맥루가 있는 쪽 팔에 발생했다. 혈액투석 시작 후 시술까지의 기간은 중앙값 28(0.5-180)개월이었다. 중심정맥 삽관의 평균 사용기간은 중앙값 35일이었고, 병변이 있는 쪽 팔의 동정맥루의 평균 사용기간은 중앙값 15.1개월이었다. 모든 환자에서 스텐트를 성공적으로 시술하였고 시술 직후부터 상완의 부종이 감소하였다. 병변 부위는 좌측 쇄골하정맥 2명, 좌측 상완두정맥 7명, 우측 상완두정맥 1명이었다. 3명의 환자에서 시술 후 재협착이 발생하여 풍선 성형술을 다시 시행하여 모두 성공적으로 치료하였다. Stenosis of the subclavian vein after cannulation occurs in 15-50% of chronic hemodialysis patients, and impedes the placement of an arteriovenous fistula in the ipsilateral arm. Its natural history and pathogenic mechanism are not well established yet. To investigate the clinical characteristics of the patients and therapeutic effect of percutaneous angioplasty and stenting, 10 consecutive chronic hemodialysis patients(3 men and 7 women; mean 55 year old) from March 1999 to February 2001 who showed subclavian vein stenosis and were treated with above percutaneous procedure were included in this study. The patients were followed for median 15.8(9.3-23.7) months after stenting. The causes of chronic renal failure were hypertension in four, diabetes mellitus in four, and unknown in two patients. Edema was noted on left upper extremity in nine and right in one patient. All cases of stenosis of central vein were found in ipsilateral side. Duration from beginning of hemodialysis to angioplasty and stenting was median 28(0.5-180) months. Duration for keeping subclavian vein catheter was median 35(13-65) days and duration for arteriovenous fistula in ipsilateral arm was median 15.1(1.1-120) months before stenting. All patients were performed stenting successfully and edema of upper extremities began to decreased immediately after the procedure. Involved stenosis lesions were noted on left bracheocephalic vein in seven, left subclavian vein in two, and right bracheocephalic vein in one patient. Restenosis after the procedure occurred in three patients in 1 year after stenting, and percutaneous balloon angioplasty(198, 256, 276 days after initial stenting) was performed again successfully. There was no severe complication during the procedure in all patients. In conclusion, we speculate that percutaneous angioplasty and stenting is a safe and effective therapeutic modality in chronic hemodialysis patients with central vein stenosis.

위장관계 악성종양에서 CA 125 측정의 의미

장우익 ( Jang U Ig ),안강현 ( An Gang Hyeon ),이종인 ( Lee Jong In ),김현수 ( Kim Hyeon Su ),김효열 ( Kim Hyo Yeol ),김수경 ( Kim Su Gyeong ),이동기 ( Lee Dong Gi ),배선우 ( Bae Seon U ),심영학 ( Sim Yeong Hag ),강명서 ( Kang Myeo 대한내과학회 1993 대한내과학회지 Vol.44 No.4

연구배경 : 종양표지자는 암외 진단, 임상적 병기구분 및 치료효과 판정에 유용하며, 위장관암에서 alphafetoprotein, carcinoembryonic antigen, CA 19-9, 및 CA 72-4 등이 중요한 지표로 사용되고 있으나 위암의 경우, 현재까지 만족할 만한 유용성을 가진 종양표지자는 없는 실정이다. 저자등은 위암에 대한 새로운 종양표지자로 CA 125의 역할을 규명하고자 위암을 비롯한 각종 위장관암에서 CA 125와 기존의 종양표지자와의 유용성을 검토하였다. 방법 : 대상은 위장관 악성종양 181예, 위장관 양성질환자 55예를 대상으로 하였으며 CA 72-4는 CIS ELSA CA 72-4 RIA kit, CA 19-9는 Abbott CA 19-9 RIA kit, CEA는 Abbott CEA kit, CA 125는 Centocor CA 125 RIA kit를 사용하여 측정하였으며, 각각의 정상치 한계는 4 U/ml, 37 U/ml, 5ng/ml그리고 35 U/ml로 하였다. 결과 : 1) CA 125는 양성 소화기 질환에서 93%의 높은 특이도를 보였으나 복수가 있는 간경변증의 경우 거의 전예에서 혈중 CA 125값이 증가하여 악성종양군과 감별이 어려웠다. 2) CA 125는 위암에서 민감도가 36%로 CA 72-4와 차이가 없었다. 3) CA 125는 췌담도암에 대한 민감도가 55%로 CA 19-9의 85%에 비해 낮았으며 두 종양표지자를 병합하여 사용한 경우 민감도의 증가(95%)를 볼 수 있었다. 4) 23예의 조기위암 환자에서 CA 125의 민감도는 9%로 낮았다. 5) Conventional image study 상으로 인지되지 않은 복강내 암종증 환자에 대한 CA 125의 민감도는 36%였다. 결론 : CA 125는 위암에서 기존의 종양표지자인 CA 72-4와 유용성의 차이를 보이지 않았으며 전이성 위암에서 높은 민감도를 나타내었다. 혈중 CA 125의 증가는 위장관의 악성 및 양성질환에 관계없이 복수의 유무와 밀접한 연관관계를 가지는 것으로 사료되며 통상적인 영상 진단법상으로 인지되지 않은 복강내 암종중의 예견에 있어서는 유용하지 않을 것으로 생각된다. Background: Detection of tumor markers could offer an accessible method for screening risk groups in order to achieve an early diagnosis of cansers, to contribute to and adequate staging, and to help evaluate effects of therapy. CA 125 is an antigenic determinant defined by murine monoclonal antibody OC 125, and synthesized by coelomic epithelial derivatives such as Mullerian epithelium and mesothelial tissues. CA 125 is regarded as useful tumor marker for non-musinous epithelial ovarian tumors. So we investigated whether CA 125 is reliable tumor marker for gastrointestinal malignancies and especially for detection of intraperitoneal carcinomatosis unrecognized by conventional imaging studies. And we compared CA 125 with other conventional tumor markers in the serodiagnosis of gastrointestinal malignancies. Methods: Serum CA 72-4, CA 19-9, CA 125 and CEA were determined radioimmunologically with monoclonal antibodies. A cut-off value of 4 U/ml, 37 U/ml, 35 U/ml and 5 mg/ml were used respectively. Results: The results are summarized as follows. 1) CA 125 had high specificity (93%) for benign gastrointestinal disease but was not useful to differetiate malignant ascites and benign ascites. 2) In gastric carcinoma, the sensitivity of CA 125 (36%) was not so different to that of CA 72-4(36%). 3) In pancreatobiliary carcinoma, the sensitivity of CA 125(55%) was inferior than that of CA 19-9 (85%), but combined use of these two tumor markers increases sensitivitu(95%) for the diagnosis. 4) The sensitivities of CA 125 for 23 early gastric cancer was 9%. 5) The sensitivities of CA 125 for the intraperitoneal carcinomatosis that was not recognized by conventional imaging studies was 36%. Conclustion: Our data indicates that CA 125 is not better in the serodiagnosis of gastrointestinal malignancies than other conventional tumor markers, and CA 125 has a little predictive value in the diagnosis of intraperitoneal carcinomatosis unrecognized by the conventional imaging studies. Serum CA 125 elevation in benign or malignant diseases closely correlated with the presence of ascites.

Comparative Analysis of Detection Methods for Food-borne Pathogens in Fresh-cut Agricultural Materials

Hye-Jeong Jang(장혜정),Hye-Jeong Kim(김혜정),Ji-in Park(박지인),Sun-Nyoung Yu(유선녕),Bo-Bae Park(박보배),Gang-Ja Ha(하강자),Soon-Cheol Ahn(안순철),Dong-Seob Kim(김동섭) 한국생명과학회 2021 생명과학회지 Vol.31 No.1

건강에 대한 관심 증대와 1인 가구 증가라는 사회구조적인 변화로 이용하기 편리한 농산물에 대한 소비가 증가하고 있다. 대부분의 신선 농산물은 가열하지 않고 섭취하는 경우가 많기 때문에 식품 매개 병원체에 쉽게 노출될 수 있어 세계적으로 과채류가 원인인 식중독 사고의 보고가 증가하고 있다. 이에 본 연구에서는 신선 농산물의 미생물학적 품질을 평가하고 식중독균 검출 방법을 비교 분석하고자 하였다. 신선 농산물 중 채소류 129건을 구입하여 배양기반 방법으로 식중독균을 분석한 결과, non-pathogenic Escherichia coli (3.9%), Bacillus cereus (31.8%), Clostridium perfringens (5.4%), Yersinia enterocolitica (0.8%), enterohemorrhagic E. coli (0.8%)가 검출되었다. 이러한 식중독균의 분석에는 증균 배양과정이 중요하게 작용을 하며 균주의 순수 분리 및 확인 동정에까지 상대적으로 많은 시간과 노력이 요구된다. 따라서 증균 배양의 과정 없이 식중독균을 신속하게 검출 할 수 있는 PCRDGGE를 수행하여 배양 기반의 분석법과 비교하였다. 비병원성 대장균은 배양 기반 방법에서 검출되지 않았음에도 PCR-DGGE에서는 검출된 경우가 2건이 있었다. 본 연구에서 사용한 대장균 정량 분석방법은 시료를 10배 희석한 후 배양하는 과정에서 시료의 손실 가능성과 검출 한계가 높은 단점으로 PCR-DGGE가 균종의 확인에 더욱 용이할 것으로 보였다. 저위해성 식중독균은 배양 기반 방법보다 PCR-DGGE에서 검출 한계가 높은 것으로 보였다. 고위해성 식중독균은 배양 기반 방법보다 PCR-DGGE (10 CFU/g)에서 검출 한계가 낮아 균종 확인과 검출에 용이하다고 판단되었고 이를 통해 채소류에서 고위해성 식중독균의 잠재적 위험성을 확인하였다. 본 연구의 결과는 신선 농산물의 미생물 위해 평가와 기준 설정을 위한 기초 자료로 활용될 수 있으며 신선 농산물 관련 식중독균 검출 방법의 개선과 식중독 발생 예방에 기여할 것으로 기대한다. The consumption of fresh-cut agricultural materials is increasing due to increased public interest in health and the increase of single-person households. Most fresh-cut agricultural materials can be eaten without heating, thus easily exposing the consumer to food-borne pathogens. As a result, food-borne diseases are increasing worldwide. In the analysis of food-borne pathogens, it is important to detect the strains, but this is time consuming and laborious. Alternative detection methods that have been introduced, include polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), which is performed without prior culturing. Samples of fresh-cut agricultural materials, such as vegetables, were analyzed by the culture-based method. In 129 samples, non-pathogenic Escherichia coli (3.9%), Bacillus cereus (31.8%), Clostridium perfringens (5.4%), Yersinia enterocolitica (0.8%), and enterohemorrhagic E. coli (0.8%) were detected. Eight samples contaminated with bacteria were randomly selected, further analyzed by PCR-DGGE, and compared with the culture-based method. Two cases detected non-pathogenic E. coli by PCR-DGGE only, despite a lack of detection by the culture method. It was supposed there was possibility of sample loss during its 10-fold dilution for appropriate cultivation. In the detection of high-risk food-borne pathogens, it was found that the detection limit was lower in PCR-DGGE than in the culture-based method (10 CFU/g). This suggests that PCR-DGGE can be alternatively used to detect strains. On the other hand, low-risk food-borne pathogens seem to have higher detection limits in PCR-DGGE. Consequentially, this study contributes to the improvement of food-borne pathogen detection and the prevention of its related-diseases in fresh-cut agricultural materials.

불안정 흉벽손상에서 지속적 인공호흡법과 내적 늑골고정술의 비교

강창희,장인성,Gang, Chang-Hui,Jang, In-Seong 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery (J Chest Surg) Vol.30 No.4

순천향대학교 의과대학 흉부외과학교실에서는 1992년 1월부터 1996년 6월까지 불안정 흉벽손상으로 지속적 인공호흡법의 치료를 받은 22례와 내적 늑골고정 술을 받은 15례를 대상으로 하여 비교분석하였 다. 환자의 나이는 지속적 인공호흡군이 평균 $43.4\pm16.4세었고수술정복군이$ 평균 $40.9\pm13.8세었으$ 며, 성 비는 지속적 인공호흡군이 6.3 : 1, 수술정복군이 6.5 : 1으로 두 군 모두 사회활동이 활발한 30,40 대 남자에서 많은 분포를 보였으며 두 군간에 현저한 차이는 없었다. 늑골골절 수는 지속적 인공호홉군이 평균 $8.7\pm2.3개,$ 수술정복군이 평균 $7.5\pm2.5개로$ 두 군간에는 통계학적 차이가 없었으며(p=0.3129) 동 반된 흉막, 폐장 및 심장의 손상정도가 두 군간에 차이를 보이지 않았다. 인공호흡기의 사용기간은 지속 적 인공호흡군이 $8.7\pm3.3일,$ 수술정복군이 $5.7\pm1.7일으로$ 두 군간에는 통계학적 유의성이 있었으며 (p=0.0134),중환자실의 입원기간은 지속적 인공호흡군이 $13.2\pm4.1일,수술정복군이$ 8.3그3.9일으로두 군간에 의미있는 차이가 있었다(p=0.0175). 합병증은 지속적 인공호흡군이 무기폐 7례(31.8%), 폐렴 4 례(18.2%), 농흉 2례(9.1%) 및 인공호흡과 관련된 합병증 3례(1).6% ; 기흉 2례, 외상후 폐기종 1례)가 있었으며,수술정복군은 무기폐 3례(20.0%),폐렴 1례(6.7%),수술창의 문제 2례(12.3%; 감염 1례,파열 I례) 및 인공호흡과 관련된 기흉 1례(6.7%)가 발생되어 지속적 인공호흡군이 수술정복군보다 합병증의 발생이 많았다 사망은 지속적 인공호흉군이 5명(22.7%),수술정복군이 2명(13.3%)으로 지속적 인공호 흡군에서 사망율이 높았다. From January, 1992 to June, 1996, )7 patients with flail chest were treated at Sonnchunhyang university hospital. 15 patients were managed by internal fixation of fractured ribs, whereas the remaining 22 patients were managed by endotracheal intubation and intermittent positive-pressure ventilation alone. There were no difference between two groups in age, sex, the severity of injury to the chest wall and the nature of associated injuries. Average dur'Btion of assisted ventilation was 5.7 $\pm$ 1.7 days in the patients treated by internal fixation versus 8.7 $\pm$ 3.3 days In the patients treated by continuous me hanical ventilation. Average stay in the intensive care unit was 8.3 $\pm$ 3.9 days for the patients treated by internal fixation, whereas it was $13.2\pm4.1$ days in the group treated by continuous mechanical ventilation alone. In the group treated by internal fixation, complications were 3 atelectases(20.0%), 1 pneumonia(6.7%), 2 operative wound problems(12.3%) and 1 barotrauma(6.7%). In the other group, 7 atelectases(31.8%), 4 pneumonitis(18.2%), 2 empyemas(9.1%) and 3 barotraumas(1).6%). The mortality rate was 13.3%(2/15) in the surgically treated patients, whereas it was 22.7%(5122) in the other group. The treatment of flail chest by internal fixation resulted in speedy recovery, decreased complications and mortalities, and better ultimatc cosmetic and functional results.

HBV : O-001 ; An observational, multicenter, cohort study evaluating the antiviral efficacy, safety, and tolerability in Korean patients with chronic hepatitis B (CHB) receiving pegylated Interferon-alpha 2a (Pegasys®): TRACES STUDY

( Young Eun Chon ),( Dong Joon Kim ),( Sang Gyune Kim ),( In Hee Kim ),( Si Hyun Bae ),( Seong Gyu Hwang ),( Jeong Heo ),( Jeong Won Jang ),( Byung Seok Lee ),( Hyung Joon Kim ),( Dae Won Jun ),( Gang 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Until today, there is currently no data available on the efficacy, safety, and tolerability of pegylated interferon alfa 2a (PEG-IFNa-2a) in Korean patients with chronic hepatitis B (CHB), who are known to have mostly HBV genotype C. Methods: We collected data from 18 institutes of 451 Korean patients with CHB who were treated with PEG-IFNa-2a as a first line therapy (370 patients with HBeAg positive and 81 with HBeAg negative). Treatment responses at the end of treatment (ET) and at 6 months post-treatment (PT6) were compared between patients treated for 24 weeks vs. 48 weeks, and adverse events were evaluated. Results: In HBeAg-positive patients, the patients who had received PEG-IFNa-2a for 48 weeks than 24 weeks achieved significantly higher virological response (HBV DNA < 2,000 IU/mL) (48 vs. 24 weeks at ET, 44.4% vs. 27.2%, p = 0.005; at PT6, 46.7% vs. 17.2%, p=0.001). Complete virological response (HBV DNA < 60 IU/mL) at ET was also achieved in more patients with a longer treatment (48 vs. 24 weeks 22.0% vs. 13.0%, p=0.005). HBeAg seroconversion rate at ET was 18.1% with 48 weeks treatment, which is significantly higher than 10.1% (p=0.012) with 24 weeks treatment. This finding was continued to PT6 (23.7% vs. 17.2%, p = 0.028). The rate of ALT normalization was increased from 61.4% at ET to 76.8% at PT6 on 48 weeks-treatment. HBsAg seroconversion was not common (48 vs. 24 weeks at ET, 0.4% vs. 0%; at PT6, 0.8% vs. 0%). In HBeAg-negative patients, virologic response at ET was higher than that in HBeAg-positive patients following 48 weeks treatment (serum HBV DNA< 60 and < 2,000 IU/mL; 60.8% and 87.8%, respectively). HBsAg seroconversion was 1.4% at ET and it was maintained at PT6. Adverse events were typical of those associated with PEG-IFNa-2a. Conclusion: In Korean CHB patients, PEG-IFNa-2a showed substantial treatment response and good tolerability. In patients with HBeAg-positive CHB, the longer treatment of PEG-IFNa-2a (48 weeks rather than 24 weeks) was more efficacious with similar safety profiles.

HBV : O-001 ; An observational, multicenter, cohort study evaluating the antiviral efficacy, safety, and tolerability in Korean patients with chronic hepatitis B (CHB) receiving pegylated Interferon-alpha 2a (Pegasys®): TRACES STUDY

( Young Eun Chon ),( Dong Joon Kim ),( Sang Gyune Kim ),( In Hee Kim ),( Si Hyun Bae ),( Seong Gyu Hwang ),( Jeong Heo ),( Jeong Won Jang ),( Byung Seok Lee ),( Hyung Joon Kim ),( Dae Won Jun ),( Gang 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Until today, there is currently no data available on the efficacy, safety, and tolerability of pegylated interferon alfa 2a (PEG-IFNa-2a) in Korean patients with chronic hepatitis B (CHB), who are known to have mostly HBV genotype C. Methods: We collected data from 18 institutes of 451 Korean patients with CHB who were treated with PEG-IFNa-2a as a first line therapy (370 patients with HBeAg positive and 81 with HBeAg negative). Treatment responses at the end of treatment (ET) and at 6 months post-treatment (PT6) were compared between patients treated for 24 weeks vs. 48 weeks, and adverse events were evaluated. Results: In HBeAg-positive patients, the patients who had received PEG-IFNa-2a for 48 weeks than 24 weeks achieved significantly higher virological response (HBV DNA < 2,000 IU/mL) (48 vs. 24 weeks at ET, 44.4% vs. 27.2%, p = 0.005; at PT6, 46.7% vs. 17.2%, p=0.001). Complete virological response (HBV DNA < 60 IU/mL) at ET was also achieved in more patients with a longer treatment (48 vs. 24 weeks 22.0% vs. 13.0%, p=0.005). HBeAg seroconversion rate at ET was 18.1% with 48 weeks treatment, which is significantly higher than 10.1% (p=0.012) with 24 weeks treatment. This finding was continued to PT6 (23.7% vs. 17.2%, p = 0.028). The rate of ALT normalization was increased from 61.4% at ET to 76.8% at PT6 on 48 weeks-treatment. HBsAg seroconversion was not common (48 vs. 24 weeks at ET, 0.4% vs. 0%; at PT6, 0.8% vs. 0%). In HBeAg-negative patients, virologic response at ET was higher than that in HBeAg-positive patients following 48 weeks treatment (serum HBV DNA< 60 and < 2,000 IU/mL; 60.8% and 87.8%, respectively). HBsAg seroconversion was 1.4% at ET and it was maintained at PT6. Adverse events were typical of those associated with PEG-IFNa-2a. Conclusion: In Korean CHB patients, PEG-IFNa-2a showed substantial treatment response and good tolerability. In patients with HBeAg-positive CHB, the longer treatment of PEG-IFNa-2a (48 weeks rather than 24 weeks) was more efficacious with similar safety profiles.

Identification of candidate genomic region associated with seed longevity by re-sequencing in rice

In-Seon Jeong,Jun-Hyeon Cho,Chang-Deok Han,Hyun-Ju Lee,Hyeon-So Ji,Gang-Seob Lee,Ung-Han Yoon,Jang-Ho Hahn,Tae-Ho Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

It is well known that Dharial (Bangladesh origin and weedy rice line) has longer seed longevity than indica and japonica rice varieties. To study the genetic basis of seed longevity of Dharial, we developed 240 BC3F7 backcross recombinant inbred lines derived from the crosses between Dharial (a donor parent) and two korea rice accessions (recurrent parents) including Ilmi and Gopum, respectively. Among these lines, we selected two introgression lines with longer seed longevity and named them Ilmi-NIL and Gopum-NIL. Also, we developed an EMS-induced mutant line from Dharial which has shortened seed longevity, and named it Dharial-EMS. We performed re-sequencing of four rice accessions that are Dharial, Dharial-EMS, Ilmi-NIL, and Gopum-NIL. A total of 706×106 raw reads were generated which provided sequence data over 46x rice genome coverage per each accession. We did genome-wide variation analysis comparing produced re-sequencing data and the re-sequencing data of Ilmi from NABIC database with the Nipponbare reference sequence. By graphical analysis of SNP distribution in rice genome of the five accessions, we could select candidate chromosomal segments introgressed from Dharial in Ilmi-NIL and Gopum-NIL. The introgressed chromosomal segments were in seven regions in Ilmi-NIL and eight regions in Gopum-NIL, and four common introgressed regions between Ilmi-NIL and Gopum-NIL were identified. 2,758 SNPs between Dharial and Dharial-EMS were found in the introgressed regions. Also, we detected 450 genes including at least one SNP among these SNPs. This result will facilitate identification of genes and development of molecular markers for improvement of seed longevity.

SNP-based analysis of genetic diversity in anther-derived rice by whole genome sequencing

Jeong, In-Seon,Yoon, Ung-Han,Lee, Gang-Seob,Ji, Hyeon-So,Lee, Hyun-Ju,Han, Chang-Deok,Hahn, Jang-Ho,An, Gynheung,Kim, Tae-Ho Springer New York 2013 Rice Vol.6 No.-

<P><B>Background</B></P><P>Anther culture has advantage to obtain a homozygous progeny by induced doubling of haploid chromosomes and to improve selection efficiency for invaluable agronomical traits. Therefore, anther culturing is widely utilized to breed new varieties and to induce genetic variations in several crops including rice. Genome sequencing technologies allow the detection of a massive number of DNA polymorphism such as SNPs and Indels between closely related cultivars. These DNA polymorphisms permit the rapid identification of genetic diversity among cultivars and genomic locations of heritable traits. To estimate sequence diversity derived from anther culturing, we performed whole-genome resequencing of five Korean rice accessions, including three anther culture lines (BLB, HY-04 and HY-08), their progenitor cultivar (Hwayeong), and an additional japonica cultivar (Dongjin).</P><P><B>Results</B></P><P>A total of 1,165 × 10<SUP>6</SUP> raw reads were generated with over 58× coverage that detected 1,154,063 DNA polymorphisms between the Korean rice accessions and <I>Nipponbare</I>. We observed that in Hwayeong and its progenies, 0.64 SNP was found per one kb of <I>Nipponbare</I> genome, while Dongjin, bred by a conventional breeding method, had a lower number of SNPs (0.45 SNP/kb). Among 1,154,063 DNA polymorphisms, 29,269 non-synonymous SNPs located on 30,013 genes and these genes were functionally classified based on gene ontology (GO). We also analyzed line-specific SNPs which were estimated 1 ~ 3% of the total SNPs. The frequency of non-synonymous SNPs in each accession ranged from 26 SNPs in Hwayeong to 214 SNPs in HY-04.</P><P><B>Conclusions</B></P><P>The genetic difference we detected between the progenies derived from anther culture and their mother cultivar is due to somaclonal variation during tissue culture process, such as karyotype change, chromosome rearrangement, gene amplification and deletion, transposable element, and DNA methylation. Detection of genome-wide DNA polymorphisms by high-throughput sequencer enabled to identify sequence diversity derived from anther culturing and genomic locations of heritable traits. Furthermore, it will provide an invaluable resource to identify molecular markers and genes associated with diverse traits of agronomical importance.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/1939-8433-6-6) contains supplementary material, which is available to authorized users.</P>

신증후군에 합병된 총장골동맥과 관상동맥의 혈전증

장윤경 ( Jang Yun Gyeong ),최대은 ( Choe Dae Eun ),양종오 ( Yang Jong O ),구영선 ( Gu Yeong Seon ),강민규 ( Kang Min Gyu ),황평주 ( Hwang Pyeong Ju ),성인환 ( Seong In Hwan ),이강욱 ( Lee Gang Ug ),신영태 ( Sin Yeong Tae ) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.6

Thrombosis is one of the important complications of nephrotic syndrome. Still, the mechanism of thrombosis of nephrotic syndrome is under debate, but the presence of hypercoagulable state in nephrotic syndrome is regarded as the principal contributing factor to that. Venous thrombosis in nephrotic syndrome has been increasingly reported, but arterial thrombosis is relatively rare and has been reported mainly in nephrotic children. Authors experienced a 35-year old male patient with frequently relapsing nephrotic syndrome of focal segmental glomerulosclerosis, who complained sudden onset of claudication, cold sensation, numbness and cyanosis of right lower extremity. A diagnosis of thrombosis in right common iliac artery and thromboses in septal and distal branch of left anterior descending coronary artery was made by arterial angiography. The patient fully recovered after emergent thrombectomy and anticoagulation therapy.