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( Yi Fan Hong ),( Hangeun Kim ),( Hye Rim Kim ),( Min Geun Gim ),( Dae Kyun Chung ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.12
It is known that lactic acid bacteria (LAB) have many beneficial health effects, including antioxidative activity and immune regulation. In this study, the immune regulatory effects of Lactobacillus sakei and Lactobacillus plantarum, which are found in different types of kimchi, were evaluated. L. sakei and its lipoteichoic acid (LTA) have greater immune stimulating potential in IL-12, IFN-γ, and TNF-α production as compared with L. plantarum in an in vitro condition. On the other hand, L. plantarum is assumed to repress the Th1 immune response in murine experiments. After being injected with LPS, L. plantarum-fed mice maintained a healthier state, and the level of TNF-α in their blood was lower than in other bacterial strainfed mice and in the LPS-only control mice. Additionally, IL-12 production was significantly decreased and the production of IL-4 was greatly increased in the splenocytes from L. plantarum-fed mice. Further experiments revealed that the pre-injection of purified LTA from L. plantarum (pLTA), L. sakei (sLTA), and S. aureus (aLTA) decreased TNF-α and IL-4 production in LPS-injected mice. Mouse IL-12, however, was significantly increased by aLTA pre-injection. In conclusion, the L. sakei and L. plantarum strains have immune regulation effects, but the effects differ in cytokine production and the regulatory effects of the Th1/Th2 immune response.
Effects of Food Components on the Antibacterial Activity of Chitosan against Escherichia coli
Yi Fan Hong,Eunpyo Moon,Yun Hee Park 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.6
The antibacterial activity of chitosan against Escherichia coli was investigated in the presence of NaCl, sucrose, and ethanol to assess the potential use of chitosan as a biopreservative in food products containing these components. The inhibitory activity of chitosan decreased slightly upon the addition of NaCl and sucrose, respectively to culture broth containing 100 ppm of chitosan (Mw 3,000), while the addition of ethanol enhanced the inhibitory activity of chitosan on growing cells. The addition of these components to non-growing cells prior to chitosan treatment demonstrated that NaCl protected the cells from the inhibitory activity of chitosan, while sucrose had no effect. Ethanol addition to non-growing cells increased cell death by chitosan treatment. Finally, binding of fluorescein isothiocyanate (FITC)-labeled chitosan to E. coli was measured in the presence of the food components. The FITC-labeled chitosan binding to cells decreased upon NaCl addition, was not affected by sucrose, and increased following treatment with ethanol.
( Yi-fan Hong ),( Yoon-doo Lee ),( Jae-yeon Park ),( Seongjae Kim ),( Youn-woo Lee ),( Boram Jeon ),( Deepa Jagdish ),( Hangeun Kim ),( Dae Kyun Chung ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Lactic acid bacteria (LAB) have beneficial effects on intestinal health and skin diseases. Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is known to induce the production of several cytokines such as TNF-α, IL-1β, and IL-8 and affect the intestinal microflora, anti-aging, sepsis, and cholesterol level. In this study, Weissella cibaria was isolated from Indian dairy products, and we examined its immune-enhancing effects. Live and heatkilled W. cibaria did not induce the secretion of immune-related cytokines, whereas LTA isolated from W. cibaria (cLTA) significantly increased the secretion of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. cLTA increased the phosphorylation of nuclear factor kappalight-chain-enhancer of activated B cells, p38 mitogen-activated protein kinases, and c-Jun Nterminal kinases in THP-1 cells. The secretion of TNF-α and IL-6 was also increased in the cLTA-treated mouse splenocytes. These results suggest that cLTA, but not W. cibaria whole cells, has immune-boosting potential and can be used to treat immunosuppression diseases.
Hong, Yi-Fan,Lee, Yoon-Doo,Park, Jae-Yeon,Kim, Seongjae,Lee, Youn-Woo,Jeon, Boram,Jagdish, Deepa,Kim, Hangeun,Chung, Dae Kyun The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
Lactic acid bacteria (LAB) have beneficial effects on intestinal health and skin diseases. Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is known to induce the production of several cytokines such as TNF-α, IL-1β, and IL-8 and affect the intestinal microflora, anti-aging, sepsis, and cholesterol level. In this study, Weissella cibaria was isolated from Indian dairy products, and we examined its immune-enhancing effects. Live and heat-killed W. cibaria did not induce the secretion of immune-related cytokines, whereas LTA isolated from W. cibaria (cLTA) significantly increased the secretion of TNF-α, IL-1β, and IL-6 in a dose-dependent manner. cLTA increased the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells, p38 mitogen-activated protein kinases, and c-Jun N-terminal kinases in THP-1 cells. The secretion of TNF-α and IL-6 was also increased in the cLTA-treated mouse splenocytes. These results suggest that cLTA, but not W. cibaria whole cells, has immune-boosting potential and can be used to treat immunosuppression diseases.
Immune Regulatory Effect of Newly Isolated Lactobacillus delbrueckii from Indian Traditional Yogurt
( Yi Fan Hong ),( Yoon Doo Lee ),( Jae Yeon Park ),( Boram Jeon ),( Deepa Jagdish ),( Soo Jin Jang ),( Dae Kyun Chung ),( Han Geun Kim ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8
Lactic acid bacteria (LAB) are microorganisms that are believed to provide health benefits. Here, we isolated LAB from Indian fermented foods, such as traditional Yogurt and Dosa. LAB from Yogurt most significantly induced TNF-α and IL-1β production, whereas LAB from Dosa induced mild cytokine production. After 16S rRNA gene sequencing and phylogenetic analysis, a Yogurt-borne lactic acid bacterium was identified and classified as Lactobacillus delbrueckii subsp. bulgaricus, and it was renamed L. delbrueckii K552 for the further studies. Our data suggest that the newly isolated L. delbrueckii can be used for the treatment of immune deficiency disorders.
키토산의 항균활성에 미치는 염화나트륨 및 유기산의 영향
홍의범 ( Yi Fan Hong ),김한근 ( Hangeun Kim ),방면호 ( Myun Ho Bang ),김현수 ( Hyun Su Kim ),김태락 ( Tae Rack Kim ),박윤희 ( Yun Hee Park ),정대균 ( Dae Kyun Chung ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.4
식품용 방부제로써의 가능성을 평가하기 위하여 염화나트륨, 아세트산, 젖산, 구연산이 포함된 배지를 사용하여 대장균 및 황색포도 상구균에 대한 키토산의 항균성을 조사하였다. 키토산의 억제활성은 100 ppm의 키토산(분자량 3,000)이 포함된 배양배지에 염화나트륨을 첨가시킴으로써 미세하게 감소하였다. 반면, 아세트산, 젖산, 구연산 등은 세균의 성장에 대한 키토산의 억제활성을 증가시켰다. 이러한 결과는 염화나트륨, 아세트산, 젖산, 구연산과 같은 식품 첨가 성분이 키토산의 항균활성에 심각한 영향을 미칠 수 있다는 것을 보여준다. The antibacterial activity of chitosan against Escherichia coli and Staphylococcus aureus was investigated in the presence of NaCl, acetic acid, lactic acid, and citric acid to assess its potential use as a food preservative. The inhibitory activity of chitosan decreased slightly upon adding NaCl to culture broth containing 100 ppm of chitosan (MW 3,000), while adding acetic acid, lactic acid, or citric acid enhanced the inhibitory activity of chitosan on growing cells. Our results indicate that food components, such as NaCl, acetic acid, lactic acid, and citric acid, can significantly affect the bactericidal activity of chitosan.
范振洪,李炳鎭 평택대학교 1999 論文集 Vol.13 No.-
本文??述了跨世紀韓國與中國山동省經貿合作的前景問題. (1) 跨世紀韓國與中國山東省經貿合作的有利條件 制約要所 (2) 跨世紀韓國與中國山東省經貿合作的重点 ; (3) 跨世紀韓國與中國山東省經貿合作的對策.
Fan Zeyang,Yi Hong,Xu Jian,Xie Kun 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3
Passivity-based design gains much popularity in grid-connected inverters (GCIs) since it enables system stability regardless of the uncertain grid impedance. This paper devotes to a systematic passivity-based design guidance for the LCL-fltered GCI with inverter current control and capacitor-current active damping. It is found that the passivity can be guaranteed with an optimal damping gain of the capacitor-current active damping, but it sufers from both the flter parameter drift and the lagging phase of the current regulator, especially at the one-sixth sampling frequency. To address this issue, a phase-lead compensation is introduced in series with the current regulator, which helps to enhance the full-band passivity and high robustness against the aforementioned challenge. Experiments are conducted to confrm the theoretical expectation.